FvH4_3g26690

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001922
Physical Location & Seq
Forward (+)
19795998 .. 19805359
9362 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g26690.t1

Sequence Viewer

Length: 732 bp
ATGGCAGACCCTTCCCGACCGGTCACCAGCTACCCAGCCCCTCCCTACGCCCAGCCCTACCCTCAGCCCGCCGCCGCCGCCAACGGCCACCCCAACGGCGCCTACCCAAACTATCCTGTCTACCAGCAAGCCTCGTACAACGCGTACACCGCACGCGCCGCCTTCGTCCGCCGCTTCGTCATCGCCATGGTCTTCCTCTTCGTCATCTTCGGCTCCCTCCTCCTCATAATCTGGCTCATCCTCCGCCCCAAGATCCCTGACTTCCGAGTCGACTCGTTTACAGTGACGAATCTCAACGTCTCCTCCTCCCGCCAAGCCCTCACCGGAACCTGGTCCGTCGGCTTCGACGTCTTCAACCCCAACAAGAAGATGACCATCGAGTACGACGGGATCGGCGCCTCCGTCTTCTACCGCTCTGGCTTCATCTCCGAGACAAGGGTCCCGCCGTTCCAGCAGGGCAAGCGCGTCCGGAGCGCAGTGAACGCGACTCTCTCGGCGGCGAACTCGTTCGTCGATGACAGGGTGGCGAGTGGGATCAACGCGGACAGGGCGGCGCGTGGGCTGGTTAGCTTCAATATCAAGCTGCTGGCCAGGGTTCAGTTCCGGAAAGGCGGGTGGAGGCTGAGGCGGCGGCTGCTGAGAGTGTTGTGCCTTGACGTGCCGGTTGCCATTTCGAATAATGGGACCGGGAAGATGACCGGCGGAGGTAGAGATTGCGGCGTTGCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

244

Amino Acids

26.64

Weight (kDa)

10.55

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
AatII GACGTC 1 cut(s) 351
AccB1I GGYRCC 2 cut(s) 98, 395
AccBSI CCGCTC 1 cut(s) 414
AccI GTMKAC 2 cut(s) 120, 270
AccII CGCG 6 cut(s) 143, 156, 465, 485, 542, 556
AccIII TCCGGA 2 cut(s) 468, 603
AclWI GGATC 3 cut(s) 247, 398, 542
AcoI YGGCCR 2 cut(s) 85, 588
AcyI GRCGYC 3 cut(s) 99, 348, 396
AfaI GTAC 3 cut(s) 137, 146, 383
AfiI CCNNNNNNNGG 2 cut(s) 330, 435
AflIII ACRYGT 1 cut(s) 141
AgeI ACCGGT 1 cut(s) 19
AgsI TTSAA 2 cut(s) 355, 574
AjiI CACGTC 1 cut(s) 658
AjnI CCWGG 2 cut(s) 329, 590
AluBI AGCT 3 cut(s) 30, 570, 583
AluI AGCT 3 cut(s) 30, 570, 583
Alw26I GTCTC 2 cut(s) 304, 425
AlwI GGATC 3 cut(s) 247, 398, 542
Aor13HI TCCGGA 2 cut(s) 468, 603
AoxI GGCC 2 cut(s) 85, 588
ApeKI GCWGC 2 cut(s) 583, 634
AsiGI ACCGGT 1 cut(s) 19
Asp700I GAANNNNTTC 1 cut(s) 506
AspLEI GCGC 6 cut(s) 101, 158, 398, 465, 476, 556
AspS9I GGNCC 3 cut(s) 333, 439, 684
AsuC2I CCSGG 1 cut(s) 688
AsuHPI GGTGA 2 cut(s) 16, 313
AsuII TTCGAA 1 cut(s) 674
AvaII GGWCC 3 cut(s) 333, 439, 684
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 2 cut(s) 98, 395
BbsI GAAGAC 3 cut(s) 184, 343, 397
BbvCI CCTCAGC 2 cut(s) 63, 623
BbvI GCAGC 2 cut(s) 570, 621
BccI CCATC 1 cut(s) 383
BceAI ACGGC 3 cut(s) 100, 112, 430
BciT130I CCWGG 2 cut(s) 331, 592
BcnI CCSGG 1 cut(s) 688
BcoDI GTCTC 2 cut(s) 304, 425
BfoI RGCGCY 2 cut(s) 102, 399
Bme1390I CCNGG 3 cut(s) 331, 592, 688
Bme18I GGWCC 3 cut(s) 333, 439, 684
BmgBI CACGTC 1 cut(s) 658
BmgT120I GGNCC 3 cut(s) 333, 439, 684
BmiI GGNNCC 7 cut(s) 100, 214, 328, 397, 440, 441, 685
BmrFI CCNGG 3 cut(s) 331, 592, 688
BoxI GACNNNNGTC 1 cut(s) 437
BpiI GAAGAC 3 cut(s) 184, 343, 397
Bpu10I CCTNAGC 2 cut(s) 63, 623
Bpu14I TTCGAA 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 688
BsaBI GATNNNNATC 1 cut(s) 374
BsaHI GRCGYC 3 cut(s) 99, 348, 396
BsaJI CCNNGG 2 cut(s) 186, 591
BsaWI WCCGGW 4 cut(s) 19, 323, 468, 603
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 330, 435
Bse118I RCCGGY 3 cut(s) 19, 661, 698
Bse8I GATNNNNATC 1 cut(s) 374
BseAI TCCGGA 2 cut(s) 468, 603
BseBI CCWGG 2 cut(s) 331, 592
BseDI CCNNGG 2 cut(s) 186, 591
BseGI GGATG 1 cut(s) 237
BseJI GATNNNNATC 1 cut(s) 374
BseLI CCNNNNNNNGG 2 cut(s) 330, 435
BseMII CTCAG 3 cut(s) 77, 614, 629
BseRI GAGGAG 4 cut(s) 209, 212, 292, 295
BseXI GCAGC 2 cut(s) 570, 621
BseYI CCCAGC 2 cut(s) 34, 51
Bsh1236I CGCG 6 cut(s) 143, 156, 465, 485, 542, 556
Bsh1285I CGRYCG 1 cut(s) 20
BshFI GGCC 2 cut(s) 87, 590
BshNI GGYRCC 2 cut(s) 98, 395
BshTI ACCGGT 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 20
BsiSI CCGG 7 cut(s) 20, 324, 469, 604, 662, 687, 699
BslFI GGGAC 2 cut(s) 425, 697
BslI CCNNNNNNNGG 2 cut(s) 330, 435
BsmAI GTCTC 2 cut(s) 304, 425
BsmBI CGTCTC 1 cut(s) 304
BsmFI GGGAC 2 cut(s) 425, 697
BsnI GGCC 2 cut(s) 87, 590
Bsp119I TTCGAA 1 cut(s) 674
Bsp13I TCCGGA 2 cut(s) 468, 603
Bsp143I GATC 3 cut(s) 252, 390, 534
Bsp19I CCATGG 1 cut(s) 186
BspANI GGCC 2 cut(s) 87, 590
BspCNI CTCAG 3 cut(s) 76, 615, 630
BspEI TCCGGA 2 cut(s) 468, 603
BspFNI CGCG 6 cut(s) 143, 156, 465, 485, 542, 556
BspLI GGNNCC 7 cut(s) 100, 214, 328, 397, 440, 441, 685
BspPI GGATC 3 cut(s) 247, 398, 542
BspT104I TTCGAA 1 cut(s) 674
BspT107I GGYRCC 2 cut(s) 98, 395
BsrBI CCGCTC 1 cut(s) 414
BsrFI RCCGGY 3 cut(s) 19, 661, 698
BssAI RCCGGY 3 cut(s) 19, 661, 698
BssECI CCNNGG 2 cut(s) 186, 591
BssMI GATC 3 cut(s) 252, 390, 534
BssNI GRCGYC 3 cut(s) 99, 348, 396
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 331, 592
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 3 cut(s) 99, 348, 396
BstBI TTCGAA 1 cut(s) 674
BstC8I GCNNGC 5 cut(s) 69, 129, 154, 461, 588
BstDEI CTNAG 3 cut(s) 63, 623, 638
BstDSI CCRYGG 1 cut(s) 186
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 1 cut(s) 237
BstFNI CGCG 6 cut(s) 143, 156, 465, 485, 542, 556
BstH2I RGCGCY 2 cut(s) 102, 399
BstHHI GCGC 6 cut(s) 101, 158, 398, 465, 476, 556
BstKTI GATC 3 cut(s) 255, 393, 537
BstMAI GTCTC 2 cut(s) 304, 425
BstMBI GATC 3 cut(s) 252, 390, 534
BstMCI CGRYCG 1 cut(s) 20
BstNI CCWGG 2 cut(s) 331, 592
BstPAI GACNNNNGTC 1 cut(s) 437
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 3 cut(s) 329, 590, 686
BstUI CGCG 6 cut(s) 143, 156, 465, 485, 542, 556
BstV1I GCAGC 2 cut(s) 570, 621
BstV2I GAAGAC 3 cut(s) 184, 343, 397
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BsuRI GGCC 2 cut(s) 87, 590
BtgI CCRYGG 1 cut(s) 186
BtgZI GCGATG 1 cut(s) 166
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 288, 483
Cac8I GCNNGC 5 cut(s) 69, 129, 154, 461, 588
CfoI GCGC 6 cut(s) 101, 158, 398, 465, 476, 556
Cfr10I RCCGGY 3 cut(s) 19, 661, 698
Cfr13I GGNCC 3 cut(s) 333, 439, 684
CseI GACGC 1 cut(s) 454
CsiI ACCWGGT 1 cut(s) 329
Csp6I GTAC 3 cut(s) 136, 145, 382
CspAI ACCGGT 1 cut(s) 19
CviAII CATG 1 cut(s) 187
CviQI GTAC 3 cut(s) 136, 145, 382
DdeI CTNAG 3 cut(s) 63, 623, 638
DinI GGCGCC 2 cut(s) 100, 397
DpnI GATC 3 cut(s) 254, 392, 536
DpnII GATC 3 cut(s) 252, 390, 534
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 2 cut(s) 85, 588
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
EciI GGCGGA 3 cut(s) 158, 233, 717
Eco130I CCWWGG 1 cut(s) 186
Eco47I GGWCC 3 cut(s) 333, 439, 684
Eco91I GGTNACC 1 cut(s) 22
EcoO109I RGGNCCY 1 cut(s) 439
EcoO65I GGTNACC 1 cut(s) 22
EcoRII CCWGG 2 cut(s) 329, 590
EcoT14I CCWWGG 1 cut(s) 186
EgeI GGCGCC 2 cut(s) 100, 397
EheI GGCGCC 2 cut(s) 100, 397
ErhI CCWWGG 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 1 cut(s) 190
FaiI YATR 2 cut(s) 188, 227
FaqI GGGAC 2 cut(s) 425, 697
FatI CATG 1 cut(s) 186
FauI CCCGC 4 cut(s) 76, 317, 450, 605
FblI GTMKAC 2 cut(s) 120, 270
FokI GGATG 1 cut(s) 224
GlaI GCGC 6 cut(s) 100, 157, 397, 464, 475, 555
GsaI CCCAGC 2 cut(s) 38, 55
HaeII RGCGCY 2 cut(s) 102, 399
HaeIII GGCC 2 cut(s) 87, 590
HapII CCGG 7 cut(s) 20, 324, 469, 604, 662, 687, 699
HgaI GACGC 1 cut(s) 454
HhaI GCGC 6 cut(s) 101, 158, 398, 465, 476, 556
Hin1I GRCGYC 3 cut(s) 99, 348, 396
Hin1II CATG 1 cut(s) 190
Hin6I GCGC 6 cut(s) 99, 156, 396, 463, 474, 554
HinP1I GCGC 6 cut(s) 99, 156, 396, 463, 474, 554
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 4 cut(s) 267, 272, 289, 487
HpaII CCGG 7 cut(s) 20, 324, 469, 604, 662, 687, 699
HphI GGTGA 2 cut(s) 16, 313
Hpy166II GTNNAC 5 cut(s) 121, 147, 271, 279, 481
Hpy188I TCNGA 2 cut(s) 266, 430
Hpy188III TCNNGA 3 cut(s) 15, 469, 604
Hpy8I GTNNAC 5 cut(s) 121, 147, 271, 279, 481
Hpy99I CGWCG 4 cut(s) 341, 350, 389, 515
HpyAV CCTTC 2 cut(s) 21, 172
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 3 cut(s) 297, 348, 657
HpyF3I CTNAG 3 cut(s) 63, 623, 638
HpySE526I ACGT 3 cut(s) 297, 348, 657
Hsp92I GRCGYC 3 cut(s) 99, 348, 396
Hsp92II CATG 1 cut(s) 190
HspAI GCGC 6 cut(s) 99, 156, 396, 463, 474, 554
KasI GGCGCC 2 cut(s) 98, 395
KflI GGGWCCC 1 cut(s) 439
Kpn2I TCCGGA 2 cut(s) 468, 603
Kzo9I GATC 3 cut(s) 252, 390, 534
LmnI GCTCC 2 cut(s) 218, 471
Lsp1109I GCAGC 2 cut(s) 570, 621
MabI ACCWGGT 1 cut(s) 329
MaeII ACGT 3 cut(s) 297, 348, 657
MaeIII GTNAC 2 cut(s) 22, 283
MalI GATC 3 cut(s) 254, 392, 536
MbiI CCGCTC 1 cut(s) 414
MboI GATC 3 cut(s) 252, 390, 534
MboII GAAGA 7 cut(s) 184, 190, 199, 343, 379, 397, 703
MflI RGATCY 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 590
MluI ACGCGT 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 590
Mly113I GGCGCC 2 cut(s) 99, 396
MlyI GAGTC 3 cut(s) 266, 276, 481
Mox20I TGGCCA 1 cut(s) 590
MroI TCCGGA 2 cut(s) 468, 603
MroXI GAANNNNTTC 1 cut(s) 506
MscI TGGCCA 1 cut(s) 590
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 590
MspI CCGG 7 cut(s) 20, 324, 469, 604, 662, 687, 699
MspR9I CCNGG 3 cut(s) 331, 592, 688
MvaI CCWGG 2 cut(s) 331, 592
MvnI CGCG 6 cut(s) 143, 156, 465, 485, 542, 556
NarI GGCGCC 2 cut(s) 99, 396
NciI CCSGG 1 cut(s) 688
NcoI CCATGG 1 cut(s) 186
NdeII GATC 3 cut(s) 252, 390, 534
NlaIII CATG 1 cut(s) 190
NlaIV GGNNCC 7 cut(s) 100, 214, 328, 397, 440, 441, 685
NmeAIII GCCGAG 1 cut(s) 473
NmuCI GTSAC 2 cut(s) 22, 283
NspV TTCGAA 1 cut(s) 674
PcsI WCGNNNNNNNCGW 3 cut(s) 140, 345, 399
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 289
PinAI ACCGGT 1 cut(s) 19
PleI GAGTC 3 cut(s) 266, 275, 481
PluTI GGCGCC 2 cut(s) 102, 399
PpsI GAGTC 3 cut(s) 266, 275, 481
PpuMI RGGWCCY 1 cut(s) 439
PshAI GACNNNNGTC 1 cut(s) 437
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 2 cut(s) 329, 590
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 2 cut(s) 34, 51
PspGI CCWGG 2 cut(s) 329, 590
PspN4I GGNNCC 7 cut(s) 100, 214, 328, 397, 440, 441, 685
PspPI GGNCC 3 cut(s) 333, 439, 684
PspPPI RGGWCCY 1 cut(s) 439
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 3 cut(s) 137, 146, 383
RsaNI GTAC 3 cut(s) 136, 145, 382
RseI CAYNNNNRTG 1 cut(s) 185
SalI GTCGAC 1 cut(s) 269
Sau3AI GATC 3 cut(s) 252, 390, 534
Sau96I GGNCC 3 cut(s) 333, 439, 684
SchI GAGTC 3 cut(s) 266, 276, 481
ScrFI CCNGG 3 cut(s) 331, 592, 688
SetI ASST 8 cut(s) 32, 300, 332, 351, 572, 585, 660, 709
SexAI ACCWGGT 1 cut(s) 329
SfoI GGCGCC 2 cut(s) 100, 397
SfuI TTCGAA 1 cut(s) 674
SinI GGWCC 3 cut(s) 333, 439, 684
SmiMI CAYNNNNRTG 1 cut(s) 185
SspDI GGCGCC 2 cut(s) 98, 395
StyD4I CCNGG 3 cut(s) 329, 590, 686
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 3 cut(s) 300, 351, 660
TaqI TCGA 5 cut(s) 270, 345, 378, 513, 674
TfiI GAWTC 1 cut(s) 289
TscAI CASTG 2 cut(s) 288, 483
TseFI GTSAC 2 cut(s) 22, 283
TseI GCWGC 2 cut(s) 583, 634
Tsp45I GTSAC 2 cut(s) 22, 283
TspDTI ATGAA 1 cut(s) 412
TspGWI ACGGA 2 cut(s) 325, 391
TspRI CASTG 2 cut(s) 288, 483
VpaK11BI GGWCC 3 cut(s) 333, 439, 684
XmiI GTMKAC 2 cut(s) 120, 270
XmnI GAANNNNTTC 1 cut(s) 506
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.