RchiOBHm_Chr5g0047591

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001922
Physical Location & Seq
Reverse (-)
44061072 .. 44062221
1150 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32547

Sequence Viewer

Length: 567 bp
ATGATTTGGAAAAGGTTGCTTCATCATCGCCATGGTCTTCCTCTTCGTCATCATCGGCTCCTTCCTCCTCATGATCTGGCTCATCCTCCGCCCCAAGATCCCCGACTTCCGAGTCGACTCCTTCACCGTCACCAACCTCAACGTCTCCTCCTCCCGCCAGTCCCTCACCGGCACCTGGTCCGTAGGCTTCGACGTCTTCAACCCCAACAAGAAGATGACCATCGAGTACGACGAGATCGGCTCCTCCGTCTTCTACCGCTCCGGCTTCATTTCCGAGACCCAAATCCCGCCGTTCCGGGAGTCCGCAGCGCCGTGAACGCGACCTTCTCCGCGGCGAACTCGTTCGTCGGCGACAGGGTGGCGAGTGGGATCGACGCGGACAGGGCGGCGCGTGGGGCGGTGAGTTTCAATATCAAGCTGCTGGCGAGGGTTCAGTTCCGGAAAGGCGGGTGGAGGCTGAGGCGGCGGCTGCTGAGGGTGCTGTGCCGTGACGTGCCGGTTACGATTTCGAATAATGGGACCGGGATGATGACCGGCGGAGGTAGAGAATGCGGCGTTGCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

188

Amino Acids

21.57

Weight (kDa)

12.0

Isoelectric Point (pI)

65.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 111
AatII GACGTC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 171
AccBSI CCGCTC 1 cut(s) 259
AccI GTMKAC 1 cut(s) 115
AccII CGCG 4 cut(s) 320, 332, 377, 391
AccIII TCCGGA 1 cut(s) 438
AclWI GGATC 2 cut(s) 92, 377
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 175
AgsI TTSAA 2 cut(s) 200, 409
AjiI CACGTC 1 cut(s) 493
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 1 cut(s) 418
AluI AGCT 1 cut(s) 418
Alw26I GTCTC 2 cut(s) 149, 270
AlwI GGATC 2 cut(s) 92, 377
Aor13HI TCCGGA 1 cut(s) 438
ApeKI GCWGC 3 cut(s) 306, 418, 469
Asp700I GAANNNNTTC 1 cut(s) 341
AspLEI GCGC 2 cut(s) 311, 391
AspS9I GGNCC 2 cut(s) 178, 519
AsuC2I CCSGG 2 cut(s) 297, 523
AsuHPI GGTGA 4 cut(s) 116, 122, 158, 412
AsuII TTCGAA 1 cut(s) 509
AvaII GGWCC 2 cut(s) 178, 519
BanI GGYRCC 1 cut(s) 171
BbsI GAAGAC 3 cut(s) 29, 188, 242
BbvCI CCTCAGC 2 cut(s) 458, 473
BbvI GCAGC 3 cut(s) 318, 405, 456
BccI CCATC 1 cut(s) 228
BceAI ACGGC 3 cut(s) 275, 296, 471
BciT130I CCWGG 1 cut(s) 176
BcnI CCSGG 2 cut(s) 297, 523
BcoDI GTCTC 2 cut(s) 149, 270
BfoI RGCGCY 1 cut(s) 312
BisI GCNGC 8 cut(s) 307, 333, 387, 419, 464, 467, 470, 553
BlsI GCNGC 8 cut(s) 308, 334, 388, 420, 465, 468, 471, 554
Bme1390I CCNGG 3 cut(s) 176, 297, 523
Bme18I GGWCC 2 cut(s) 178, 519
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 2 cut(s) 178, 519
BmiI GGNNCC 4 cut(s) 59, 173, 242, 520
BmrFI CCNGG 3 cut(s) 176, 297, 523
BpiI GAAGAC 3 cut(s) 29, 188, 242
BplI GAGNNNNNCTC 2 cut(s) 225, 257
Bpu10I CCTNAGC 2 cut(s) 458, 473
Bpu14I TTCGAA 1 cut(s) 509
BpuMI CCSGG 2 cut(s) 297, 523
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 2 cut(s) 31, 330
BsaWI WCCGGW 1 cut(s) 438
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse118I RCCGGY 3 cut(s) 168, 496, 533
Bse1I ACTGG 1 cut(s) 158
Bse8I GATNNNNATC 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 438
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 2 cut(s) 31, 330
BseGI GGATG 2 cut(s) 82, 531
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 449, 464
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 4 cut(s) 57, 137, 140, 233
BseXI GCAGC 3 cut(s) 318, 405, 456
Bsh1236I CGCG 4 cut(s) 320, 332, 377, 391
BshNI GGYRCC 1 cut(s) 171
BsiSI CCGG 7 cut(s) 169, 262, 296, 439, 497, 522, 534
BslFI GGGAC 2 cut(s) 146, 532
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 2 cut(s) 149, 270
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 2 cut(s) 146, 532
BsmI GAATGC 1 cut(s) 554
Bso31I GGTCTC 1 cut(s) 270
Bsp119I TTCGAA 1 cut(s) 509
Bsp13I TCCGGA 1 cut(s) 438
Bsp143I GATC 4 cut(s) 73, 97, 235, 369
Bsp19I CCATGG 1 cut(s) 31
BspCNI CTCAG 2 cut(s) 450, 465
BspEI TCCGGA 1 cut(s) 438
BspFNI CGCG 4 cut(s) 320, 332, 377, 391
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 4 cut(s) 59, 173, 242, 520
BspPI GGATC 2 cut(s) 92, 377
BspT104I TTCGAA 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 171
BspTNI GGTCTC 1 cut(s) 270
BsrBI CCGCTC 1 cut(s) 259
BsrFI RCCGGY 3 cut(s) 168, 496, 533
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 3 cut(s) 168, 496, 533
BssECI CCNNGG 2 cut(s) 31, 330
BssMI GATC 4 cut(s) 73, 97, 235, 369
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 1 cut(s) 128
Bst6I CTCTTC 1 cut(s) 48
BstACI GRCGYC 1 cut(s) 193
BstBI TTCGAA 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 2 cut(s) 458, 473
BstDSI CCRYGG 2 cut(s) 31, 330
BstF5I GGATG 2 cut(s) 82, 531
BstFNI CGCG 4 cut(s) 320, 332, 377, 391
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 311, 391
BstKTI GATC 4 cut(s) 76, 100, 238, 372
BstMAI GTCTC 2 cut(s) 149, 270
BstMBI GATC 4 cut(s) 73, 97, 235, 369
BstMWI GCNNNNNNNGC 6 cut(s) 317, 383, 395, 463, 469, 478
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 3 cut(s) 174, 295, 521
BstUI CGCG 4 cut(s) 320, 332, 377, 391
BstV1I GCAGC 3 cut(s) 318, 405, 456
BstV2I GAAGAC 3 cut(s) 29, 188, 242
BstX2I RGATCY 1 cut(s) 97
BstYI RGATCY 1 cut(s) 97
BtgI CCRYGG 2 cut(s) 31, 330
BtgZI GCGATG 1 cut(s) 11
BtrI CACGTC 1 cut(s) 493
BtsCI GGATG 2 cut(s) 82, 531
Cac8I GCNNGC 1 cut(s) 423
CciI TCATGA 1 cut(s) 70
CfoI GCGC 2 cut(s) 311, 391
Cfr10I RCCGGY 3 cut(s) 168, 496, 533
Cfr13I GGNCC 2 cut(s) 178, 519
Cfr42I CCGCGG 1 cut(s) 333
CseI GACGC 1 cut(s) 383
CsiI ACCWGGT 1 cut(s) 174
Csp6I GTAC 1 cut(s) 227
CviAII CATG 2 cut(s) 32, 71
CviJI RGCY 8 cut(s) 58, 80, 187, 241, 265, 418, 457, 469
CviKI_1 RGCY 8 cut(s) 58, 80, 187, 241, 265, 418, 457, 469
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 2 cut(s) 458, 473
DpnI GATC 4 cut(s) 75, 99, 237, 371
DpnII GATC 4 cut(s) 73, 97, 235, 369
DrdI GACNNNNNNGTC 1 cut(s) 111
DseDI GACNNNNNNGTC 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
EciI GGCGGA 2 cut(s) 78, 552
Eco130I CCWWGG 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 178, 519
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 2 cut(s) 35, 74
FaiI YATR 2 cut(s) 33, 72
FaqI GGGAC 2 cut(s) 146, 532
FatI CATG 2 cut(s) 31, 70
FauI CCCGC 3 cut(s) 162, 295, 440
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 8 cut(s) 307, 333, 387, 419, 464, 467, 470, 553
FokI GGATG 2 cut(s) 69, 538
Fsp4HI GCNGC 8 cut(s) 307, 333, 387, 419, 464, 467, 470, 553
GlaI GCGC 2 cut(s) 310, 390
GluI GCNGC 8 cut(s) 307, 333, 387, 419, 464, 467, 470, 553
HaeII RGCGCY 1 cut(s) 312
HapII CCGG 7 cut(s) 169, 262, 296, 439, 497, 522, 534
HgaI GACGC 1 cut(s) 383
HhaI GCGC 2 cut(s) 311, 391
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 2 cut(s) 35, 74
Hin6I GCGC 2 cut(s) 309, 389
HinP1I GCGC 2 cut(s) 309, 389
HincII GTYRAC 1 cut(s) 116
HindII GTYRAC 1 cut(s) 116
HinfI GANTC 3 cut(s) 112, 117, 300
HpaII CCGG 7 cut(s) 169, 262, 296, 439, 497, 522, 534
HphI GGTGA 4 cut(s) 116, 122, 158, 412
Hpy166II GTNNAC 2 cut(s) 116, 316
Hpy188I TCNGA 3 cut(s) 111, 275, 566
Hpy188III TCNNGA 2 cut(s) 71, 439
Hpy8I GTNNAC 2 cut(s) 116, 316
Hpy99I CGWCG 4 cut(s) 195, 234, 350, 377
HpyAV CCTTC 3 cut(s) 71, 131, 334
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 3 cut(s) 142, 193, 492
HpyF10VI GCNNNNNNNGC 6 cut(s) 317, 383, 395, 463, 469, 478
HpyF3I CTNAG 2 cut(s) 458, 473
HpySE526I ACGT 3 cut(s) 142, 193, 492
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 2 cut(s) 35, 74
HspAI GCGC 2 cut(s) 309, 389
Kpn2I TCCGGA 1 cut(s) 438
KspI CCGCGG 1 cut(s) 333
Kzo9I GATC 4 cut(s) 73, 97, 235, 369
LmnI GCTCC 3 cut(s) 63, 246, 264
Lsp1109I GCAGC 3 cut(s) 318, 405, 456
MabI ACCWGGT 1 cut(s) 174
MaeII ACGT 3 cut(s) 142, 193, 492
MaeIII GTNAC 3 cut(s) 128, 488, 499
MalI GATC 4 cut(s) 75, 99, 237, 371
MbiI CCGCTC 1 cut(s) 259
MboI GATC 4 cut(s) 73, 97, 235, 369
MboII GAAGA 5 cut(s) 29, 35, 188, 224, 242
MflI RGATCY 1 cut(s) 97
MlyI GAGTC 3 cut(s) 111, 121, 309
MroI TCCGGA 1 cut(s) 438
MroXI GAANNNNTTC 1 cut(s) 341
MslI CAYNNNNRTG 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 7 cut(s) 169, 262, 296, 439, 497, 522, 534
MspR9I CCNGG 3 cut(s) 176, 297, 523
Mva1269I GAATGC 1 cut(s) 554
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 4 cut(s) 320, 332, 377, 391
MwoI GCNNNNNNNGC 6 cut(s) 317, 383, 395, 463, 469, 478
NciI CCSGG 2 cut(s) 297, 523
NcoI CCATGG 1 cut(s) 31
NdeII GATC 4 cut(s) 73, 97, 235, 369
NlaIII CATG 2 cut(s) 35, 74
NlaIV GGNNCC 4 cut(s) 59, 173, 242, 520
NmuCI GTSAC 2 cut(s) 128, 488
NspV TTCGAA 1 cut(s) 509
PagI TCATGA 1 cut(s) 70
PcsI WCGNNNNNNNCGW 2 cut(s) 229, 244
PctI GAATGC 1 cut(s) 554
PdmI GAANNNNTTC 1 cut(s) 341
PfoI TCCNGGA 1 cut(s) 295
PkrI GCNGC 8 cut(s) 308, 334, 388, 420, 465, 468, 471, 554
PleI GAGTC 3 cut(s) 111, 120, 308
PpsI GAGTC 3 cut(s) 111, 120, 308
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 4 cut(s) 59, 173, 242, 520
PspPI GGNCC 2 cut(s) 178, 519
PsuI RGATCY 1 cut(s) 97
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
RseI CAYNNNNRTG 1 cut(s) 30
SacII CCGCGG 1 cut(s) 333
SalI GTCGAC 1 cut(s) 114
SatI GCNGC 8 cut(s) 307, 333, 387, 419, 464, 467, 470, 553
Sau3AI GATC 4 cut(s) 73, 97, 235, 369
Sau96I GGNCC 2 cut(s) 178, 519
SchI GAGTC 3 cut(s) 111, 121, 309
ScrFI CCNGG 3 cut(s) 176, 297, 523
SetI ASST 9 cut(s) 17, 139, 145, 177, 196, 326, 420, 495, 544
SexAI ACCWGGT 1 cut(s) 174
Sfr303I CCGCGG 1 cut(s) 333
SfuI TTCGAA 1 cut(s) 509
SgrBI CCGCGG 1 cut(s) 333
SinI GGWCC 2 cut(s) 178, 519
SmiMI CAYNNNNRTG 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 174, 295, 521
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 3 cut(s) 145, 196, 495
TaqI TCGA 5 cut(s) 115, 190, 223, 372, 509
TauI GCSGC 5 cut(s) 335, 389, 466, 469, 555
TseFI GTSAC 2 cut(s) 128, 488
TseI GCWGC 3 cut(s) 306, 418, 469
Tsp45I GTSAC 2 cut(s) 128, 488
TspDTI ATGAA 2 cut(s) 11, 257
TspGWI ACGGA 2 cut(s) 170, 236
VpaK11BI GGWCC 2 cut(s) 178, 519
XmiI GTMKAC 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 341
ZraI GACGTC 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.