Rh3BG231100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001922
Physical Location & Seq
Reverse (-)
21391426 .. 21391737
312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG231100.1

Sequence Viewer

Length: 312 bp
ATGGCTACTCCGGCCGGCTACCCATCCGCCCCACCCTATCCCTATCCATCCCCTCCACCAGAGGACCTCCCCCAGGGCACAAATCCAGCCAACAACCCCCAGACCACTGGGCCAACTAGGAGTACCTCCCTCGGGGAGAACATCTGCAACTTCGTGGCCGCACTCTTCGTCTTAATTATACTCACAGCCTTGATCAGCATCACCGTTTGGATGTTCTATTCGTTAACCATGGAGAGTCCAGAACTTCCCGAGTTCAAGATCGAATCAGCCACCGTCTCGGCGTCTCCCAACTCGATACGGAAGGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

10.99

Weight (kDa)

4.55

Isoelectric Point (pI)

73.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 27, 159
AcoI YGGCCR 2 cut(s) 12, 156
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 1 cut(s) 124
AfiI CCNNNNNNNGG 2 cut(s) 73, 132
AgsI TTSAA 1 cut(s) 256
AjnI CCWGG 1 cut(s) 72
Alw26I GTCTC 2 cut(s) 280, 288
Ama87I CYCGRG 2 cut(s) 131, 248
AoxI GGCC 3 cut(s) 12, 110, 156
AspS9I GGNCC 2 cut(s) 64, 110
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 2 cut(s) 131, 248
AvaII GGWCC 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 80
BccI CCATC 2 cut(s) 31, 55
BciT130I CCWGG 1 cut(s) 74
BclI TGATCA 1 cut(s) 192
BcoDI GTCTC 2 cut(s) 280, 288
BfaI CTAG 1 cut(s) 117
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 2 cut(s) 131, 248
BmgT120I GGNCC 2 cut(s) 64, 110
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 74
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 207
BmuI ACTGGG 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 4 cut(s) 72, 73, 130, 228
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 132
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 197
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 4 cut(s) 72, 73, 130, 228
BseGI GGATG 3 cut(s) 23, 47, 216
BseJI GATNNNNATC 1 cut(s) 197
BseLI CCNNNNNNNGG 2 cut(s) 73, 132
BseNI ACTGG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 80
BseX3I CGGCCG 1 cut(s) 12
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 3 cut(s) 14, 112, 158
BsiEI CGRYCG 1 cut(s) 15
BsiHKCI CYCGRG 2 cut(s) 131, 248
BsiSI CCGG 2 cut(s) 11, 15
BslI CCNNNNNNNGG 2 cut(s) 73, 132
BsmAI GTCTC 2 cut(s) 280, 288
BsmBI CGTCTC 2 cut(s) 280, 288
BsnI GGCC 3 cut(s) 14, 112, 158
BsoBI CYCGRG 2 cut(s) 131, 248
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 2 cut(s) 192, 258
Bsp19I CCATGG 1 cut(s) 228
BspACI CCGC 2 cut(s) 27, 159
BspANI GGCC 3 cut(s) 14, 112, 158
BspLI GGNNCC 1 cut(s) 306
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 4 cut(s) 72, 73, 130, 228
BssMI GATC 2 cut(s) 192, 258
BssNI GRCGYC 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 2 cut(s) 205, 274
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 16
BstDSI CCRYGG 1 cut(s) 228
BstENI CCTNNNNNAGG 1 cut(s) 71
BstF5I GGATG 3 cut(s) 23, 47, 216
BstKTI GATC 2 cut(s) 195, 261
BstMAI GTCTC 2 cut(s) 280, 288
BstMBI GATC 2 cut(s) 192, 258
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 80
BstXI CCANNNNNNTGG 1 cut(s) 107
BstZI CGGCCG 1 cut(s) 12
BsuRI GGCC 3 cut(s) 14, 112, 158
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 3 cut(s) 23, 47, 216
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 64, 110
CseI GACGC 1 cut(s) 270
Csp6I GTAC 1 cut(s) 123
CviAII CATG 1 cut(s) 229
CviJI RGCY 9 cut(s) 5, 14, 18, 89, 112, 158, 188, 269, 305
CviKI_1 RGCY 9 cut(s) 5, 14, 18, 89, 112, 158, 188, 269, 305
CviQI GTAC 1 cut(s) 123
DpnI GATC 2 cut(s) 194, 260
DpnII GATC 2 cut(s) 192, 258
EaeI YGGCCR 2 cut(s) 12, 156
EagI CGGCCG 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EciI GGCGGA 1 cut(s) 16
EclXI CGGCCG 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 64
Eco52I CGGCCG 1 cut(s) 12
Eco88I CYCGRG 2 cut(s) 131, 248
EcoNI CCTNNNNNAGG 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 228
Esp3I CGTCTC 2 cut(s) 280, 288
FaeI CATG 1 cut(s) 232
FaiI YATR 2 cut(s) 179, 230
FatI CATG 1 cut(s) 228
FbaI TGATCA 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 3 cut(s) 10, 34, 223
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 117
GluI GCNGC 1 cut(s) 159
HaeIII GGCC 3 cut(s) 14, 112, 158
HapII CCGG 2 cut(s) 11, 15
HgaI GACGC 1 cut(s) 270
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 1 cut(s) 232
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 2 cut(s) 235, 263
HpaI GTTAAC 1 cut(s) 225
HpaII CCGG 2 cut(s) 11, 15
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 225
Hpy188III TCNNGA 3 cut(s) 239, 248, 256
Hpy8I GTNNAC 1 cut(s) 225
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 2 cut(s) 205, 274
HpyCH4V TGCA 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 1 cut(s) 232
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 192
KspAI GTTAAC 1 cut(s) 225
Kzo9I GATC 2 cut(s) 192, 258
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 9 cut(s) 24, 28, 59, 72, 86, 93, 99, 113, 252
LweI GCATC 1 cut(s) 207
MaeI CTAG 1 cut(s) 117
MalI GATC 2 cut(s) 194, 260
MboI GATC 2 cut(s) 192, 258
MboII GAAGA 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 1 cut(s) 174
MlyI GAGTC 1 cut(s) 244
MnlI CCTC 5 cut(s) 55, 63, 77, 136, 140
MroNI GCCGGC 1 cut(s) 14
MseI TTAA 2 cut(s) 173, 224
MspI CCGG 2 cut(s) 11, 15
MspR9I CCNGG 1 cut(s) 74
MvaI CCWGG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 16
NcoI CCATGG 1 cut(s) 228
NdeII GATC 2 cut(s) 192, 258
NgoMIV GCCGGC 1 cut(s) 14
NlaIII CATG 1 cut(s) 232
NlaIV GGNNCC 1 cut(s) 306
NmeAIII GCCGAG 1 cut(s) 257
PasI CCCWGGG 1 cut(s) 73
PdiI GCCGGC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 243
PpsI GAGTC 1 cut(s) 243
PpuMI RGGWCCY 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 72
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 2 cut(s) 64, 110
PspPPI RGGWCCY 1 cut(s) 64
RsaI GTAC 1 cut(s) 124
RsaNI GTAC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 173, 224
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 2 cut(s) 192, 258
Sau96I GGNCC 2 cut(s) 64, 110
SchI GAGTC 1 cut(s) 244
ScrFI CCNGG 1 cut(s) 74
SduI GDGCHC 1 cut(s) 80
SetI ASST 2 cut(s) 69, 128
SfaNI GCATC 1 cut(s) 207
SinI GGWCC 1 cut(s) 64
Sse9I AATT 1 cut(s) 174
SsiI CCGC 2 cut(s) 27, 159
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 72
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 2 cut(s) 205, 274
TaqI TCGA 2 cut(s) 261, 293
TasI AATT 1 cut(s) 174
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 2 cut(s) 173, 224
Tru9I TTAA 2 cut(s) 173, 224
TscAI CASTG 1 cut(s) 112
TspRI CASTG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 64
XagI CCTNNNNNAGG 1 cut(s) 71
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.