MD12G1038800.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001922
Physical Location & Seq
Reverse (-)
4201139 .. 4201918
780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1038800.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGCTGCTACTTCATCCGCATCGCAAGACAAGACTGTCACCGGTTATCCAACTCCGCCCGGCCAATACGCCGCCGGATACCCGGGCGCCACAGCCTATCCCTATGTGGCTGCACCGCCGCACCTCAATCCCAATACTTACCAGCCCACGGGCCCGGCACCTCTCTACTACAATCCCCAGCCCAATGGGCCCTTCAGGAGCAAACCCACCATCCTCTGCCGCATCATGATCGCCGCCGTCGCCGTCTTCACCATCATGTCCATCATCTTCTTCATCTCCTGGCTCGCCCTCCGCCCTCAACTCCCCAAGTTCCAGGTCGAATCGGCCACCGTCTCCCCGCTCAACGCGACAGGCTCCGAGCTCACCGCTACTTGGGACTTCACCCTCCTCGCCGCCAACCCCAACCACAAGCTCACCATCTTCTACGACAGACTCGAGGCCTCGCTTATATACAAGGATGAAGTTCTGCTCACCGCGGCGGCGCTGCCTCCCTTCGTCTTAGCCAAGAGGAACCAGACGCGCGTCAATATCAATCTCGCGACGGTGAGGGGGTACGTCGACGACGAGGTGGTGAGGGAAATCGCCGAAGGGAAGGATCGGGGGTCGGTGAGGTTCGGAGTGGGGGTGGCGGCGTTGGTCCGGTTTAAGTCAGGGGTGTTTCAAACGAGACGTCGGTTGTTGCGGGTCTTCTGCGATGGGGTCGAATTCACCGGGTTTGCCCGGAATAATGGGACCGGGACTTTGACGGGTCAGGCAAGTCCGTGCCAAGTTGATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

260

Amino Acids

28.15

Weight (kDa)

9.4

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 129 - 198 2.7e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 35
AatII GACGTC 1 cut(s) 673
AccB1I GGYRCC 2 cut(s) 86, 157
AccBSI CCGCTC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 558
AccII CGCG 5 cut(s) 347, 476, 520, 522, 539
AclWI GGATC 1 cut(s) 603
AcoI YGGCCR 2 cut(s) 61, 324
AcsI RAATTY 1 cut(s) 704
AcuI CTGAAG 1 cut(s) 178
AcyI GRCGYC 2 cut(s) 87, 670
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 2 cut(s) 148, 372
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 1 cut(s) 662
AjnI CCWGG 2 cut(s) 278, 312
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AluBI AGCT 2 cut(s) 361, 412
AluI AGCT 2 cut(s) 361, 412
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 337, 661
AlwI GGATC 1 cut(s) 603
Ama87I CYCGRG 2 cut(s) 82, 434
AoxI GGCC 5 cut(s) 61, 151, 188, 324, 438
ApaI GGGCCC 2 cut(s) 155, 192
ApeKI GCWGC 3 cut(s) 5, 110, 484
ApoI RAATTY 1 cut(s) 704
ArsI GACNNNNNNTTYG 4 cut(s) 655, 687, 724, 756
AsiGI ACCGGT 1 cut(s) 41
AspLEI GCGC 3 cut(s) 89, 484, 522
AspS9I GGNCC 6 cut(s) 151, 152, 188, 189, 637, 732
AsuC2I CCSGG 7 cut(s) 60, 83, 84, 155, 712, 721, 736
AvaI CYCGRG 2 cut(s) 82, 434
AvaII GGWCC 2 cut(s) 637, 732
BaeGI GKGCMC 2 cut(s) 155, 192
BanI GGYRCC 2 cut(s) 86, 157
BanII GRGCYC 3 cut(s) 155, 192, 363
BbsI GAAGAC 2 cut(s) 238, 679
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 97, 471
BccI CCATC 5 cut(s) 218, 260, 269, 425, 689
BceAI ACGGC 2 cut(s) 221, 227
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 2 cut(s) 280, 314
BciVI GTATCC 1 cut(s) 71
BcnI CCSGG 7 cut(s) 60, 83, 84, 155, 712, 721, 736
BcoDI GTCTC 2 cut(s) 337, 661
BfoI RGCGCY 2 cut(s) 90, 485
BfuI GTATCC 1 cut(s) 71
BglI GCCNNNNNGGC 1 cut(s) 187
Bme1390I CCNGG 9 cut(s) 60, 83, 84, 155, 280, 314, 712, 721, 736
Bme18I GGWCC 2 cut(s) 637, 732
BmeT110I CYCGRG 2 cut(s) 82, 434
BmgT120I GGNCC 6 cut(s) 151, 152, 188, 189, 637, 732
BmiI GGNNCC 7 cut(s) 88, 153, 159, 190, 355, 512, 733
BmrFI CCNGG 9 cut(s) 60, 83, 84, 155, 280, 314, 712, 721, 736
BmsI GCATC 2 cut(s) 29, 231
BoxI GACNNNNGTC 1 cut(s) 521
BpiI GAAGAC 2 cut(s) 238, 679
BpuMI CCSGG 7 cut(s) 60, 83, 84, 155, 712, 721, 736
BsaHI GRCGYC 2 cut(s) 87, 670
BsaJI CCNNGG 3 cut(s) 82, 147, 474
BsaWI WCCGGW 2 cut(s) 41, 639
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 372
Bse118I RCCGGY 1 cut(s) 41
BseBI CCWGG 2 cut(s) 280, 314
BseDI CCNNGG 3 cut(s) 82, 147, 474
BseGI GGATG 3 cut(s) 14, 210, 463
BseLI CCNNNNNNNGG 2 cut(s) 148, 372
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 2 cut(s) 155, 192
BseXI GCAGC 2 cut(s) 97, 471
BseYI CCCAGC 1 cut(s) 177
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 5 cut(s) 347, 476, 520, 522, 539
BshFI GGCC 5 cut(s) 63, 153, 190, 326, 440
BshNI GGYRCC 2 cut(s) 86, 157
BshTI ACCGGT 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 2 cut(s) 82, 434
BsiSI CCGG 9 cut(s) 42, 60, 75, 83, 155, 640, 711, 721, 735
BslFI GGGAC 3 cut(s) 389, 745, 751
BslI CCNNNNNNNGG 2 cut(s) 148, 372
BsmAI GTCTC 2 cut(s) 337, 661
BsmBI CGTCTC 2 cut(s) 337, 661
BsmFI GGGAC 3 cut(s) 389, 745, 751
BsnI GGCC 5 cut(s) 63, 153, 190, 326, 440
BsoBI CYCGRG 2 cut(s) 82, 434
Bsp120I GGGCCC 2 cut(s) 151, 188
Bsp1286I GDGCHC 3 cut(s) 155, 192, 363
Bsp143I GATC 2 cut(s) 228, 595
Bsp68I TCGCGA 1 cut(s) 539
BspANI GGCC 5 cut(s) 63, 153, 190, 326, 440
BspFNI CGCG 5 cut(s) 347, 476, 520, 522, 539
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 7 cut(s) 88, 153, 159, 190, 355, 512, 733
BspPI GGATC 1 cut(s) 603
BspT107I GGYRCC 2 cut(s) 86, 157
BsrBI CCGCTC 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 41
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 82, 147, 474
BssMI GATC 2 cut(s) 228, 595
BssNI GRCGYC 2 cut(s) 87, 670
Bst2UI CCWGG 2 cut(s) 280, 314
Bst4CI ACNGT 3 cut(s) 37, 331, 544
BstACI GRCGYC 2 cut(s) 87, 670
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 1 cut(s) 499
BstDSI CCRYGG 2 cut(s) 147, 474
BstF5I GGATG 3 cut(s) 14, 210, 463
BstFNI CGCG 5 cut(s) 347, 476, 520, 522, 539
BstH2I RGCGCY 2 cut(s) 90, 485
BstHHI GCGC 3 cut(s) 89, 484, 522
BstKTI GATC 2 cut(s) 231, 598
BstMAI GTCTC 2 cut(s) 337, 661
BstMBI GATC 2 cut(s) 228, 595
BstMWI GCNNNNNNNGC 2 cut(s) 187, 239
BstNI CCWGG 2 cut(s) 280, 314
BstPAI GACNNNNGTC 1 cut(s) 521
BstSCI CCNGG 9 cut(s) 58, 81, 82, 153, 278, 312, 710, 719, 734
BstSLI GKGCMC 2 cut(s) 155, 192
BstUI CGCG 5 cut(s) 347, 476, 520, 522, 539
BstV1I GCAGC 2 cut(s) 97, 471
BstV2I GAAGAC 2 cut(s) 238, 679
BstXI CCANNNNNNTGG 1 cut(s) 185
BsuI GTATCC 1 cut(s) 71
BsuRI GGCC 5 cut(s) 63, 153, 190, 326, 440
BtgI CCRYGG 2 cut(s) 147, 474
BtgZI GCGATG 2 cut(s) 6, 708
BtsCI GGATG 3 cut(s) 14, 210, 463
BtuMI TCGCGA 1 cut(s) 539
Cac8I GCNNGC 1 cut(s) 285
CciI TCATGA 1 cut(s) 225
CfoI GCGC 3 cut(s) 89, 484, 522
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 6 cut(s) 151, 152, 188, 189, 637, 732
Cfr42I CCGCGG 1 cut(s) 477
Cfr9I CCCGGG 1 cut(s) 82
CseI GACGC 2 cut(s) 511, 526
Csp6I GTAC 1 cut(s) 553
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 2 cut(s) 226, 256
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 1 cut(s) 499
DinI GGCGCC 1 cut(s) 88
DpnI GATC 2 cut(s) 230, 597
DpnII GATC 2 cut(s) 228, 595
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 2 cut(s) 61, 324
EciI GGCGGA 2 cut(s) 45, 281
Ecl136II GAGCTC 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 3 cut(s) 155, 192, 363
Eco47I GGWCC 2 cut(s) 637, 732
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 178
Eco88I CYCGRG 2 cut(s) 82, 434
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 189
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 2 cut(s) 278, 312
EcoT38I GRGCYC 3 cut(s) 155, 192, 363
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
Esp3I CGTCTC 2 cut(s) 337, 661
FaeI CATG 2 cut(s) 229, 259
FaiI YATR 5 cut(s) 105, 227, 257, 449, 451
FaqI GGGAC 3 cut(s) 389, 745, 751
FatI CATG 2 cut(s) 225, 255
FauI CCCGC 2 cut(s) 345, 675
FblI GTMKAC 1 cut(s) 558
FokI GGATG 2 cut(s) 197, 470
FriOI GRGCYC 3 cut(s) 155, 192, 363
GlaI GCGC 3 cut(s) 88, 483, 521
GsaI CCCAGC 1 cut(s) 181
HaeII RGCGCY 2 cut(s) 90, 485
HaeIII GGCC 5 cut(s) 63, 153, 190, 326, 440
HapII CCGG 9 cut(s) 42, 60, 75, 83, 155, 640, 711, 721, 735
HgaI GACGC 2 cut(s) 511, 526
HhaI GCGC 3 cut(s) 89, 484, 522
Hin1I GRCGYC 2 cut(s) 87, 670
Hin1II CATG 2 cut(s) 229, 259
Hin6I GCGC 3 cut(s) 87, 482, 520
HinP1I GCGC 3 cut(s) 87, 482, 520
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 2 cut(s) 320, 432
HpaII CCGG 9 cut(s) 42, 60, 75, 83, 155, 640, 711, 721, 735
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 2 cut(s) 358, 617
Hpy188III TCNNGA 3 cut(s) 196, 226, 538
Hpy8I GTNNAC 1 cut(s) 559
Hpy99I CGWCG 6 cut(s) 242, 544, 560, 563, 566, 675
HpyAV CCTTC 4 cut(s) 202, 502, 581, 586
HpyCH4III ACNGT 3 cut(s) 37, 331, 544
HpyCH4IV ACGT 2 cut(s) 555, 670
HpyCH4V TGCA 1 cut(s) 113
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 239
HpyF3I CTNAG 1 cut(s) 499
HpySE526I ACGT 2 cut(s) 555, 670
Hsp92I GRCGYC 2 cut(s) 87, 670
Hsp92II CATG 2 cut(s) 229, 259
HspAI GCGC 3 cut(s) 87, 482, 520
KasI GGCGCC 1 cut(s) 86
KspI CCGCGG 1 cut(s) 477
Kzo9I GATC 2 cut(s) 228, 595
LmnI GCTCC 2 cut(s) 198, 359
Lsp1109I GCAGC 2 cut(s) 97, 471
LweI GCATC 2 cut(s) 29, 231
MaeII ACGT 2 cut(s) 555, 670
MaeIII GTNAC 1 cut(s) 37
MalI GATC 2 cut(s) 230, 597
MbiI CCGCTC 1 cut(s) 340
MboI GATC 2 cut(s) 228, 595
MboII GAAGA 5 cut(s) 238, 259, 262, 412, 679
MhlI GDGCHC 3 cut(s) 155, 192, 363
MluCI AATT 1 cut(s) 704
Mly113I GGCGCC 1 cut(s) 87
MlyI GAGTC 1 cut(s) 426
MmeI TCCRAC 1 cut(s) 74
MseI TTAA 1 cut(s) 645
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 9 cut(s) 42, 60, 75, 83, 155, 640, 711, 721, 735
MspR9I CCNGG 9 cut(s) 60, 83, 84, 155, 280, 314, 712, 721, 736
MvaI CCWGG 2 cut(s) 280, 314
MvnI CGCG 5 cut(s) 347, 476, 520, 522, 539
MwoI GCNNNNNNNGC 2 cut(s) 187, 239
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 7 cut(s) 60, 83, 84, 155, 712, 721, 736
NdeII GATC 2 cut(s) 228, 595
NlaIII CATG 2 cut(s) 229, 259
NlaIV GGNNCC 7 cut(s) 88, 153, 159, 190, 355, 512, 733
NmuCI GTSAC 1 cut(s) 37
NruI TCGCGA 1 cut(s) 539
PaeR7I CTCGAG 1 cut(s) 434
PagI TCATGA 1 cut(s) 225
PceI AGGCCT 1 cut(s) 440
PcsI WCGNNNNNNNCGW 2 cut(s) 432, 561
PfeI GAWTC 1 cut(s) 320
PinAI ACCGGT 1 cut(s) 41
PleI GAGTC 1 cut(s) 426
PluTI GGCGCC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 426
PshAI GACNNNNGTC 1 cut(s) 521
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 278, 312
PspFI CCCAGC 1 cut(s) 177
PspGI CCWGG 2 cut(s) 278, 312
PspN4I GGNNCC 7 cut(s) 88, 153, 159, 190, 355, 512, 733
PspOMI GGGCCC 2 cut(s) 151, 188
PspPI GGNCC 6 cut(s) 151, 152, 188, 189, 637, 732
PspXI VCTCGAGB 1 cut(s) 434
RruI TCGCGA 1 cut(s) 539
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 363
SacII CCGCGG 1 cut(s) 477
SalI GTCGAC 1 cut(s) 557
SaqAI TTAA 1 cut(s) 645
Sau3AI GATC 2 cut(s) 228, 595
Sau96I GGNCC 6 cut(s) 151, 152, 188, 189, 637, 732
SchI GAGTC 1 cut(s) 426
ScrFI CCNGG 9 cut(s) 60, 83, 84, 155, 280, 314, 712, 721, 736
SduI GDGCHC 3 cut(s) 155, 192, 363
SetI ASST 9 cut(s) 126, 163, 318, 363, 414, 558, 570, 614, 673
SfaNI GCATC 2 cut(s) 29, 231
SfoI GGCGCC 1 cut(s) 88
Sfr274I CTCGAG 1 cut(s) 434
Sfr303I CCGCGG 1 cut(s) 477
SgrBI CCGCGG 1 cut(s) 477
SgrDI CGTCGACG 1 cut(s) 557
SinI GGWCC 2 cut(s) 637, 732
SlaI CTCGAG 1 cut(s) 434
SmaI CCCGGG 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 1 cut(s) 704
SseBI AGGCCT 1 cut(s) 440
SspDI GGCGCC 1 cut(s) 86
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 9 cut(s) 58, 81, 82, 153, 278, 312, 710, 719, 734
TaaI ACNGT 3 cut(s) 37, 331, 544
TaiI ACGT 2 cut(s) 558, 673
TaqI TCGA 4 cut(s) 318, 435, 558, 702
TasI AATT 1 cut(s) 704
TauI GCSGC 8 cut(s) 74, 121, 222, 236, 395, 479, 482, 632
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 5, 110, 484
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 262, 474
TspGWI ACGGA 1 cut(s) 750
TspMI CCCGGG 1 cut(s) 82
VpaK11BI GGWCC 2 cut(s) 637, 732
XapI RAATTY 1 cut(s) 704
XhoI CTCGAG 1 cut(s) 434
XmaI CCCGGG 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 558
ZraI GACGTC 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.