RLG00000034495

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001922
Physical Location & Seq
Reverse (-)
43745502 .. 43749476
3975 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034495

Sequence Viewer

Length: 858 bp
ATGGCAGACCCTTCTCGGCCCGTCACCGGCTACCCAGCCCCGCCCTACGCCCAGCCCTACCCCGCCAACAACGGCCAGCCCAACGGCTCCTACCCCAACTACCCAGTCTACCAGCGCGGCCCCTACAACGCGTACACCGCACGCGCCGCCTTCGTCCGCCGCTTCGTCATCGCCATGGTCTTCCTCTTCGTCATCATCGGCTCCCTCCTCCTCATGGTCTGGCTCATCCTCCGCCCCAAGATCCCCGACTTCCGAGTCGACTCGTTCACCGTCACCAACCTCAACGTCTCCTCCTCCCGCCAGTCCCTCTCCGGCACCTGGTCCGTCGGCTTCGACGTCTTCAACCCCAACAAGAAGATGACCATCGAGTACGACGAGATCGGCTCCTCCGTTTTCTACCGCTCCGGCTTCATTTCCGAGACCCAAATCCCGCCGTTCCGGCAGGGGAAGAGAGTCCGGAGCGCCGTGAACGCGACTTTCTCGGCGGCGAACTCGTTCGTCGGCGACAGGGTGGCGAGTGGGATCGACGCGGACAGGGCGGCGCGTGGGGCAGTGAGTTTCAATATCAAGCTGCTGGCTAGGGTTCAGTTCCGGAAAGGCGGGTGGAGGCTGAGGCGGCGGCTGCTGAGGGTGTTGTGCCGTGACGTGCCGGTTACGGTTTCAAAAAATGGGACCGGGACGATGACCGGCGGAGAAAAGGAGAAAAATCAGAAGCAGACTATGGAACTAGACTTTGGAGTCTTGGATACTCATAAGCCAACGCTGACTGCTGCAAGTAGAACTGCTCTTTCAAAGCAATTACAGTGTCAAATATGCTTCTTTGTGTTTCCAATGTTAAGTTTCCATGACTTCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

286

Amino Acids

31.81

Weight (kDa)

10.22

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 254, 737
AatII GACGTC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 402
AccI GTMKAC 2 cut(s) 108, 258
AccII CGCG 6 cut(s) 117, 131, 144, 473, 530, 544
AccIII TCCGGA 2 cut(s) 456, 591
AclWI GGATC 2 cut(s) 235, 530
AcoI YGGCCR 1 cut(s) 73
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 2 cut(s) 134, 371
AfiI CCNNNNNNNGG 3 cut(s) 26, 214, 318
AflIII ACRYGT 1 cut(s) 129
AgsI TTSAA 4 cut(s) 343, 562, 663, 792
AjiI CACGTC 1 cut(s) 646
AjnI CCWGG 1 cut(s) 317
AjuI GAANNNNNNNTTGG 2 cut(s) 717, 749
AluBI AGCT 1 cut(s) 571
AluI AGCT 1 cut(s) 571
Alw26I GTCTC 2 cut(s) 292, 413
AlwI GGATC 2 cut(s) 235, 530
Aor13HI TCCGGA 2 cut(s) 456, 591
AoxI GGCC 3 cut(s) 17, 73, 118
ApeKI GCWGC 3 cut(s) 571, 622, 770
Asp700I GAANNNNTTC 1 cut(s) 494
AspLEI GCGC 4 cut(s) 117, 146, 464, 544
AspS9I GGNCC 4 cut(s) 18, 119, 321, 672
AsuC2I CCSGG 1 cut(s) 676
AsuHPI GGTGA 3 cut(s) 16, 259, 265
AvaII GGWCC 2 cut(s) 321, 672
BanI GGYRCC 1 cut(s) 314
BbsI GAAGAC 2 cut(s) 172, 331
BbvCI CCTCAGC 2 cut(s) 611, 626
BbvI GCAGC 3 cut(s) 558, 609, 757
BccI CCATC 1 cut(s) 371
BceAI ACGGC 5 cut(s) 88, 100, 418, 449, 624
BciT130I CCWGG 1 cut(s) 319
BciVI GTATCC 1 cut(s) 739
BcnI CCSGG 1 cut(s) 676
BcoDI GTCTC 2 cut(s) 292, 413
BfaI CTAG 2 cut(s) 579, 728
BfoI RGCGCY 1 cut(s) 465
BfuI GTATCC 1 cut(s) 739
BglI GCCNNNNNGGC 1 cut(s) 439
Bme1390I CCNGG 2 cut(s) 319, 676
Bme18I GGWCC 2 cut(s) 321, 672
BmgBI CACGTC 1 cut(s) 646
BmgT120I GGNCC 4 cut(s) 18, 119, 321, 672
BmiI GGNNCC 6 cut(s) 88, 121, 202, 316, 385, 673
BmrFI CCNGG 2 cut(s) 319, 676
BmrI ACTGGG 1 cut(s) 98
BmuI ACTGGG 1 cut(s) 98
BpiI GAAGAC 2 cut(s) 172, 331
BplI GAGNNNNNCTC 2 cut(s) 368, 400
Bpu10I CCTNAGC 2 cut(s) 611, 626
BpuMI CCSGG 1 cut(s) 676
BsaBI GATNNNNATC 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 174
BsaWI WCCGGW 2 cut(s) 456, 591
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 214, 318
Bse118I RCCGGY 3 cut(s) 26, 649, 686
Bse1I ACTGG 2 cut(s) 104, 301
Bse8I GATNNNNATC 1 cut(s) 362
BseAI TCCGGA 2 cut(s) 456, 591
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 1 cut(s) 225
BseJI GATNNNNATC 1 cut(s) 362
BseLI CCNNNNNNNGG 3 cut(s) 26, 214, 318
BseMII CTCAG 2 cut(s) 602, 617
BseNI ACTGG 2 cut(s) 104, 301
BseRI GAGGAG 5 cut(s) 197, 200, 280, 283, 376
BseXI GCAGC 3 cut(s) 558, 609, 757
BseYI CCCAGC 2 cut(s) 34, 51
Bsh1236I CGCG 6 cut(s) 117, 131, 144, 473, 530, 544
BshFI GGCC 3 cut(s) 19, 75, 120
BshNI GGYRCC 1 cut(s) 314
BsiSI CCGG 9 cut(s) 27, 312, 405, 439, 457, 592, 650, 675, 687
BslFI GGGAC 3 cut(s) 289, 685, 691
BslI CCNNNNNNNGG 3 cut(s) 26, 214, 318
BsmAI GTCTC 2 cut(s) 292, 413
BsmBI CGTCTC 1 cut(s) 292
BsmFI GGGAC 3 cut(s) 289, 685, 691
BsnI GGCC 3 cut(s) 19, 75, 120
Bso31I GGTCTC 1 cut(s) 413
Bsp13I TCCGGA 2 cut(s) 456, 591
Bsp143I GATC 3 cut(s) 240, 378, 522
Bsp19I CCATGG 1 cut(s) 174
BspANI GGCC 3 cut(s) 19, 75, 120
BspCNI CTCAG 2 cut(s) 603, 618
BspEI TCCGGA 2 cut(s) 456, 591
BspFNI CGCG 6 cut(s) 117, 131, 144, 473, 530, 544
BspLI GGNNCC 6 cut(s) 88, 121, 202, 316, 385, 673
BspPI GGATC 2 cut(s) 235, 530
BspT107I GGYRCC 1 cut(s) 314
BspTNI GGTCTC 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 402
BsrFI RCCGGY 3 cut(s) 26, 649, 686
BsrI ACTGG 2 cut(s) 104, 301
BssAI RCCGGY 3 cut(s) 26, 649, 686
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 3 cut(s) 240, 378, 522
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 3 cut(s) 271, 658, 804
Bst6I CTCTTC 2 cut(s) 191, 443
BstACI GRCGYC 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 77, 142, 576
BstDEI CTNAG 2 cut(s) 611, 626
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 225
BstFNI CGCG 6 cut(s) 117, 131, 144, 473, 530, 544
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 4 cut(s) 117, 146, 464, 544
BstKTI GATC 3 cut(s) 243, 381, 525
BstMAI GTCTC 2 cut(s) 292, 413
BstMBI GATC 3 cut(s) 240, 378, 522
BstMWI GCNNNNNNNGC 8 cut(s) 137, 146, 439, 470, 536, 548, 616, 622
BstNI CCWGG 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 317, 674
BstUI CGCG 6 cut(s) 117, 131, 144, 473, 530, 544
BstV1I GCAGC 3 cut(s) 558, 609, 757
BstV2I GAAGAC 2 cut(s) 172, 331
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
BsuI GTATCC 1 cut(s) 739
BsuRI GGCC 3 cut(s) 19, 75, 120
BtgI CCRYGG 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 154
BtrI CACGTC 1 cut(s) 646
BtsCI GGATG 1 cut(s) 225
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 2 cut(s) 558, 809
Cac8I GCNNGC 3 cut(s) 77, 142, 576
CfoI GCGC 4 cut(s) 117, 146, 464, 544
Cfr10I RCCGGY 3 cut(s) 26, 649, 686
Cfr13I GGNCC 4 cut(s) 18, 119, 321, 672
CseI GACGC 1 cut(s) 536
CsiI ACCWGGT 1 cut(s) 317
Csp6I GTAC 2 cut(s) 133, 370
CviAII CATG 3 cut(s) 175, 214, 845
CviQI GTAC 2 cut(s) 133, 370
DdeI CTNAG 2 cut(s) 611, 626
DpnI GATC 3 cut(s) 242, 380, 524
DpnII GATC 3 cut(s) 240, 378, 522
DrdI GACNNNNNNGTC 2 cut(s) 254, 737
DseDI GACNNNNNNGTC 2 cut(s) 254, 737
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 2 cut(s) 191, 443
EarI CTCTTC 2 cut(s) 191, 443
EciI GGCGGA 3 cut(s) 146, 221, 705
Eco130I CCWWGG 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 413
Eco47I GGWCC 2 cut(s) 321, 672
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 3 cut(s) 178, 217, 848
FaiI YATR 6 cut(s) 176, 215, 722, 753, 814, 846
FaqI GGGAC 3 cut(s) 289, 685, 691
FatI CATG 3 cut(s) 174, 213, 844
FauI CCCGC 5 cut(s) 48, 70, 305, 438, 593
FblI GTMKAC 2 cut(s) 108, 258
FokI GGATG 1 cut(s) 212
FspBI CTAG 2 cut(s) 579, 728
GlaI GCGC 4 cut(s) 116, 145, 463, 543
GsaI CCCAGC 2 cut(s) 38, 55
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 3 cut(s) 19, 75, 120
HapII CCGG 9 cut(s) 27, 312, 405, 439, 457, 592, 650, 675, 687
HgaI GACGC 1 cut(s) 536
HhaI GCGC 4 cut(s) 117, 146, 464, 544
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 3 cut(s) 178, 217, 848
Hin6I GCGC 4 cut(s) 115, 144, 462, 542
HinP1I GCGC 4 cut(s) 115, 144, 462, 542
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 4 cut(s) 255, 260, 453, 738
HpaII CCGG 9 cut(s) 27, 312, 405, 439, 457, 592, 650, 675, 687
HphI GGTGA 3 cut(s) 16, 259, 265
Hpy166II GTNNAC 5 cut(s) 109, 135, 259, 267, 469
Hpy188I TCNGA 3 cut(s) 254, 418, 711
Hpy188III TCNNGA 2 cut(s) 457, 592
Hpy8I GTNNAC 5 cut(s) 109, 135, 259, 267, 469
Hpy99I CGWCG 5 cut(s) 329, 338, 377, 503, 530
HpyAV CCTTC 2 cut(s) 21, 160
HpyCH4III ACNGT 3 cut(s) 271, 658, 804
HpyCH4IV ACGT 3 cut(s) 285, 336, 645
HpyCH4V TGCA 1 cut(s) 773
HpyF10VI GCNNNNNNNGC 8 cut(s) 137, 146, 439, 470, 536, 548, 616, 622
HpyF3I CTNAG 2 cut(s) 611, 626
HpySE526I ACGT 3 cut(s) 285, 336, 645
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 3 cut(s) 178, 217, 848
HspAI GCGC 4 cut(s) 115, 144, 462, 542
Kpn2I TCCGGA 2 cut(s) 456, 591
Kzo9I GATC 3 cut(s) 240, 378, 522
LmnI GCTCC 5 cut(s) 92, 206, 389, 407, 459
Lsp1109I GCAGC 3 cut(s) 558, 609, 757
MabI ACCWGGT 1 cut(s) 317
MaeI CTAG 2 cut(s) 579, 728
MaeII ACGT 3 cut(s) 285, 336, 645
MaeIII GTNAC 4 cut(s) 22, 271, 641, 652
MalI GATC 3 cut(s) 242, 380, 524
MbiI CCGCTC 1 cut(s) 402
MboI GATC 3 cut(s) 240, 378, 522
MboII GAAGA 5 cut(s) 172, 178, 331, 367, 460
MflI RGATCY 1 cut(s) 240
MluCI AATT 1 cut(s) 797
MluI ACGCGT 1 cut(s) 129
MlyI GAGTC 4 cut(s) 254, 264, 462, 747
MroI TCCGGA 2 cut(s) 456, 591
MroXI GAANNNNTTC 1 cut(s) 494
MseI TTAA 1 cut(s) 836
MslI CAYNNNNRTG 1 cut(s) 173
MspI CCGG 9 cut(s) 27, 312, 405, 439, 457, 592, 650, 675, 687
MspR9I CCNGG 2 cut(s) 319, 676
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 6 cut(s) 117, 131, 144, 473, 530, 544
MwoI GCNNNNNNNGC 8 cut(s) 137, 146, 439, 470, 536, 548, 616, 622
NciI CCSGG 1 cut(s) 676
NcoI CCATGG 1 cut(s) 174
NdeII GATC 3 cut(s) 240, 378, 522
NlaIII CATG 3 cut(s) 178, 217, 848
NlaIV GGNNCC 6 cut(s) 88, 121, 202, 316, 385, 673
NmeAIII GCCGAG 1 cut(s) 461
NmuCI GTSAC 3 cut(s) 22, 271, 641
PcsI WCGNNNNNNNCGW 3 cut(s) 333, 372, 387
PdmI GAANNNNTTC 1 cut(s) 494
PleI GAGTC 4 cut(s) 254, 263, 461, 746
PpsI GAGTC 4 cut(s) 254, 263, 461, 746
Psp6I CCWGG 1 cut(s) 317
PspFI CCCAGC 2 cut(s) 34, 51
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 6 cut(s) 88, 121, 202, 316, 385, 673
PspPI GGNCC 4 cut(s) 18, 119, 321, 672
PsuI RGATCY 1 cut(s) 240
RsaI GTAC 2 cut(s) 134, 371
RsaNI GTAC 2 cut(s) 133, 370
RseI CAYNNNNRTG 1 cut(s) 173
SalI GTCGAC 1 cut(s) 257
SaqAI TTAA 1 cut(s) 836
Sau3AI GATC 3 cut(s) 240, 378, 522
Sau96I GGNCC 4 cut(s) 18, 119, 321, 672
SchI GAGTC 4 cut(s) 254, 264, 462, 747
ScrFI CCNGG 2 cut(s) 319, 676
SetI ASST 6 cut(s) 282, 288, 320, 339, 573, 648
SexAI ACCWGGT 1 cut(s) 317
SinI GGWCC 2 cut(s) 321, 672
SmiMI CAYNNNNRTG 1 cut(s) 173
Sse9I AATT 1 cut(s) 797
SspMI CTAG 2 cut(s) 579, 728
StyD4I CCNGG 2 cut(s) 317, 674
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 3 cut(s) 271, 658, 804
TaiI ACGT 3 cut(s) 288, 339, 648
TaqI TCGA 4 cut(s) 258, 333, 366, 525
TasI AATT 1 cut(s) 797
TauI GCSGC 7 cut(s) 120, 149, 162, 488, 542, 619, 622
Tru1I TTAA 1 cut(s) 836
Tru9I TTAA 1 cut(s) 836
TscAI CASTG 2 cut(s) 558, 809
TseFI GTSAC 3 cut(s) 22, 271, 641
TseI GCWGC 3 cut(s) 571, 622, 770
Tsp45I GTSAC 3 cut(s) 22, 271, 641
TspDTI ATGAA 1 cut(s) 400
TspGWI ACGGA 2 cut(s) 313, 379
TspRI CASTG 2 cut(s) 558, 809
VpaK11BI GGWCC 2 cut(s) 321, 672
XmiI GTMKAC 2 cut(s) 108, 258
XmnI GAANNNNTTC 1 cut(s) 494
XspI CTAG 2 cut(s) 579, 728
ZraI GACGTC 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.