MD00G1129000.v1.1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001922
Physical Location & Seq
Reverse (-)
27426110 .. 27426820
711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1129000.v1.1.491

Sequence Viewer

Length: 711 bp
ATGACCGACCCTTCTAGGCCCGTCACCGGCTACCCAGCCACGCCCTACGCCCAGCCTAACGGCGGCCACCCCTACTCCAGCTACCCCACCTACACCCAACGTCAAGGTCCCTACTCCAACGCCTATGCCGCACGCGCCACCTTCATCCGCCGCTTCGTCGGCGCCATGATCGCCCTCTTCGTCATCGTCGGCTGCATCATGCTTGTGATTTGGCTCGTCCTCCGCCCCAAGGTCCCCGACTTCCGACTCGACTCTCTCTCACTCACCAACTTCAACGTCTCCTCCTCCTCCCAGTCCCTGAGCGGCACGTGGTCCGTCGGCTTCTCCGTCTACAACCCCAACAAGAAGCTCAGCATCCGATACGAGGAGGTCGTTTCTTCCATTTTCTACCGGAACGGCTTCATATCGGAGACTCGTGTTCAGCCGTTCGCTCAGGGGAGGAAGGATCGCAATTTCGTCAACGCCACTTTGTCGGCGGTGAATTCGTTCGTGGACGCCTCGGTGGTTCGCGCGATAGAGGTGGACAGGGGACGCGGGACCGTGAGCTTTAACGTGAAGGTTTTGGCGAGGGTCCAGTTTCGGAATGGCGGGTGGAGGCTGAGGCGGCGGCTGCTGAGGGTTTTGTGCCGTGGTGTGGCGGTTTCGGTCTCTTCCAATAGCGGGTCGGGTAAGCTGGCTGGTGGATCAAGAGGTTGCCAGGTTGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

237

Amino Acids

25.95

Weight (kDa)

10.91

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 108 - 203 8.2e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 303
AccI GTMKAC 1 cut(s) 330
AccII CGCG 4 cut(s) 135, 510, 512, 534
AclWI GGATC 2 cut(s) 453, 691
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 1 cut(s) 481
AcvI CACGTG 1 cut(s) 309
AcyI GRCGYC 2 cut(s) 162, 495
AfiI CCNNNNNNNGG 6 cut(s) 26, 62, 229, 364, 634, 660
AgsI TTSAA 1 cut(s) 274
AjnI CCWGG 1 cut(s) 696
AluBI AGCT 4 cut(s) 81, 349, 546, 673
AluI AGCT 4 cut(s) 81, 349, 546, 673
Alw26I GTCTC 3 cut(s) 283, 404, 652
AlwI GGATC 2 cut(s) 453, 691
AlwNI CAGNNNCTG 1 cut(s) 298
AoxI GGCC 2 cut(s) 17, 64
ApeKI GCWGC 2 cut(s) 192, 610
ApoI RAATTY 1 cut(s) 481
Asp700I GAANNNNTTC 2 cut(s) 398, 485
AspLEI GCGC 3 cut(s) 137, 164, 512
AspS9I GGNCC 6 cut(s) 18, 107, 232, 312, 537, 571
AsuHPI GGTGA 3 cut(s) 16, 256, 490
AvaII GGWCC 5 cut(s) 107, 232, 312, 537, 571
BanI GGYRCC 1 cut(s) 161
BauI CACGAG 1 cut(s) 414
BbrPI CACGTG 1 cut(s) 309
BbvCI CCTCAGC 2 cut(s) 599, 614
BbvI GCAGC 2 cut(s) 179, 597
BceAI ACGGC 4 cut(s) 76, 409, 412, 612
BciT130I CCWGG 1 cut(s) 698
BcoDI GTCTC 3 cut(s) 283, 404, 652
BfaI CTAG 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 8 cut(s) 64, 129, 151, 193, 304, 605, 608, 611
BlpI GCTNAGC 1 cut(s) 350
BlsI GCNGC 8 cut(s) 65, 130, 152, 194, 305, 606, 609, 612
Bme1390I CCNGG 1 cut(s) 698
Bme18I GGWCC 5 cut(s) 107, 232, 312, 537, 571
BmgT120I GGNCC 6 cut(s) 18, 107, 232, 312, 537, 571
BmiI GGNNCC 5 cut(s) 109, 163, 234, 538, 572
BmrFI CCNGG 1 cut(s) 698
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 204, 363
BmuI ACTGGG 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 61
Bpu10I CCTNAGC 4 cut(s) 299, 432, 599, 614
Bpu1102I GCTNAGC 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 309
BsaHI GRCGYC 2 cut(s) 162, 495
BsaI GGTCTC 1 cut(s) 652
BsaJI CCNNGG 3 cut(s) 228, 498, 628
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 266, 296
Bsc4I CCNNNNNNNGG 6 cut(s) 26, 62, 229, 364, 634, 660
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 2 cut(s) 292, 574
BseBI CCWGG 1 cut(s) 698
BseDI CCNNGG 3 cut(s) 228, 498, 628
BseGI GGATG 2 cut(s) 144, 354
BseLI CCNNNNNNNGG 6 cut(s) 26, 62, 229, 364, 634, 660
BseMII CTCAG 5 cut(s) 290, 364, 446, 590, 605
BseNI ACTGG 2 cut(s) 292, 574
BseRI GAGGAG 4 cut(s) 271, 274, 277, 380
BseXI GCAGC 2 cut(s) 179, 597
BseYI CCCAGC 2 cut(s) 34, 51
Bsh1236I CGCG 4 cut(s) 135, 510, 512, 534
BshFI GGCC 2 cut(s) 19, 66
BshNI GGYRCC 1 cut(s) 161
BsiSI CCGG 2 cut(s) 27, 391
BslFI GGGAC 5 cut(s) 93, 218, 280, 543, 550
BslI CCNNNNNNNGG 6 cut(s) 26, 62, 229, 364, 634, 660
BsmAI GTCTC 3 cut(s) 283, 404, 652
BsmBI CGTCTC 1 cut(s) 283
BsmFI GGGAC 5 cut(s) 93, 218, 280, 543, 550
BsnI GGCC 2 cut(s) 19, 66
Bso31I GGTCTC 1 cut(s) 652
Bsp143I GATC 3 cut(s) 168, 445, 683
Bsp1720I GCTNAGC 1 cut(s) 350
BspANI GGCC 2 cut(s) 19, 66
BspCNI CTCAG 5 cut(s) 291, 363, 445, 591, 606
BspFNI CGCG 4 cut(s) 135, 510, 512, 534
BspLI GGNNCC 5 cut(s) 109, 163, 234, 538, 572
BspPI GGATC 2 cut(s) 453, 691
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 652
BsrBI CCGCTC 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 26
BsrI ACTGG 2 cut(s) 292, 574
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 3 cut(s) 228, 498, 628
BssMI GATC 3 cut(s) 168, 445, 683
BssNI GRCGYC 2 cut(s) 162, 495
BssSI CACGAG 1 cut(s) 414
BssT1I CCWWGG 1 cut(s) 228
Bst2BI CACGAG 1 cut(s) 414
Bst2UI CCWGG 1 cut(s) 698
Bst4CI ACNGT 1 cut(s) 541
Bst6I CTCTTC 2 cut(s) 182, 655
BstACI GRCGYC 2 cut(s) 162, 495
BstBAI YACGTR 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 133, 675
BstDEI CTNAG 5 cut(s) 299, 350, 432, 599, 614
BstDSI CCRYGG 1 cut(s) 628
BstF5I GGATG 2 cut(s) 144, 354
BstFNI CGCG 4 cut(s) 135, 510, 512, 534
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 3 cut(s) 137, 164, 512
BstKTI GATC 3 cut(s) 171, 448, 686
BstMAI GTCTC 3 cut(s) 283, 404, 652
BstMBI GATC 3 cut(s) 168, 445, 683
BstMWI GCNNNNNNNGC 6 cut(s) 128, 134, 159, 170, 604, 610
BstNI CCWGG 1 cut(s) 698
BstSCI CCNGG 1 cut(s) 696
BstUI CGCG 4 cut(s) 135, 510, 512, 534
BstV1I GCAGC 2 cut(s) 179, 597
BsuRI GGCC 2 cut(s) 19, 66
BtgI CCRYGG 1 cut(s) 628
BtsCI GGATG 2 cut(s) 144, 354
Cac8I GCNNGC 2 cut(s) 133, 675
CaiI CAGNNNCTG 1 cut(s) 298
CfoI GCGC 3 cut(s) 137, 164, 512
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 6 cut(s) 18, 107, 232, 312, 537, 571
CseI GACGC 2 cut(s) 503, 540
CviAII CATG 2 cut(s) 166, 199
DdeI CTNAG 5 cut(s) 299, 350, 432, 599, 614
DinI GGCGCC 1 cut(s) 163
DpnI GATC 3 cut(s) 170, 447, 685
DpnII GATC 3 cut(s) 168, 445, 683
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 182, 655
EarI CTCTTC 2 cut(s) 182, 655
EciI GGCGGA 2 cut(s) 137, 212
Eco130I CCWWGG 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 652
Eco47I GGWCC 5 cut(s) 107, 232, 312, 537, 571
Eco72I CACGTG 1 cut(s) 309
EcoO109I RGGNCCY 2 cut(s) 107, 232
EcoRI GAATTC 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 696
EcoT14I CCWWGG 1 cut(s) 228
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 2 cut(s) 169, 202
FaiI YATR 4 cut(s) 126, 167, 200, 404
FaqI GGGAC 5 cut(s) 93, 218, 280, 543, 550
FatI CATG 2 cut(s) 165, 198
FauI CCCGC 3 cut(s) 527, 581, 653
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 8 cut(s) 64, 129, 151, 193, 304, 605, 608, 611
FokI GGATG 2 cut(s) 131, 341
Fsp4HI GCNGC 8 cut(s) 64, 129, 151, 193, 304, 605, 608, 611
FspBI CTAG 1 cut(s) 15
GlaI GCGC 3 cut(s) 136, 163, 511
GluI GCNGC 8 cut(s) 64, 129, 151, 193, 304, 605, 608, 611
GsaI CCCAGC 2 cut(s) 38, 55
GsuI CTGGAG 1 cut(s) 61
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 2 cut(s) 19, 66
HapII CCGG 2 cut(s) 27, 391
HgaI GACGC 2 cut(s) 503, 540
HhaI GCGC 3 cut(s) 137, 164, 512
Hin1I GRCGYC 2 cut(s) 162, 495
Hin1II CATG 2 cut(s) 169, 202
Hin6I GCGC 3 cut(s) 135, 162, 510
HinP1I GCGC 3 cut(s) 135, 162, 510
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 3 cut(s) 246, 251, 412
HpaII CCGG 2 cut(s) 27, 391
HphI GGTGA 3 cut(s) 16, 256, 490
Hpy166II GTNNAC 4 cut(s) 331, 460, 493, 523
Hpy188I TCNGA 4 cut(s) 245, 359, 409, 582
Hpy188III TCNNGA 1 cut(s) 687
Hpy8I GTNNAC 4 cut(s) 331, 460, 493, 523
Hpy99I CGWCG 3 cut(s) 161, 191, 320
HpyAV CCTTC 4 cut(s) 21, 151, 436, 550
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4IV ACGT 4 cut(s) 100, 276, 308, 552
HpyCH4V TGCA 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 134, 159, 170, 604, 610
HpyF3I CTNAG 5 cut(s) 299, 350, 432, 599, 614
HpySE526I ACGT 4 cut(s) 100, 276, 308, 552
Hsp92I GRCGYC 2 cut(s) 162, 495
Hsp92II CATG 2 cut(s) 169, 202
HspAI GCGC 3 cut(s) 135, 162, 510
KasI GGCGCC 1 cut(s) 161
Kzo9I GATC 3 cut(s) 168, 445, 683
Lsp1109I GCAGC 2 cut(s) 179, 597
LweI GCATC 2 cut(s) 204, 363
MaeI CTAG 1 cut(s) 15
MaeII ACGT 4 cut(s) 100, 276, 308, 552
MaeIII GTNAC 1 cut(s) 22
MalI GATC 3 cut(s) 170, 447, 685
MbiI CCGCTC 1 cut(s) 303
MboI GATC 3 cut(s) 168, 445, 683
MboII GAAGA 3 cut(s) 169, 369, 642
MluCI AATT 2 cut(s) 451, 481
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 3 cut(s) 240, 245, 406
MmeI TCCRAC 2 cut(s) 141, 268
MroXI GAANNNNTTC 2 cut(s) 398, 485
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 2 cut(s) 27, 391
MspR9I CCNGG 1 cut(s) 698
MvaI CCWGG 1 cut(s) 698
MvnI CGCG 4 cut(s) 135, 510, 512, 534
MwoI GCNNNNNNNGC 6 cut(s) 128, 134, 159, 170, 604, 610
NarI GGCGCC 1 cut(s) 162
NdeII GATC 3 cut(s) 168, 445, 683
NlaIII CATG 2 cut(s) 169, 202
NlaIV GGNNCC 5 cut(s) 109, 163, 234, 538, 572
NmuCI GTSAC 1 cut(s) 22
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 324, 369
PdmI GAANNNNTTC 2 cut(s) 398, 485
PkrI GCNGC 8 cut(s) 65, 130, 152, 194, 305, 606, 609, 612
PleI GAGTC 3 cut(s) 240, 245, 406
PluTI GGCGCC 1 cut(s) 165
PmaCI CACGTG 1 cut(s) 309
PmlI CACGTG 1 cut(s) 309
PpsI GAGTC 3 cut(s) 240, 245, 406
Ppu21I YACGTR 1 cut(s) 309
PpuMI RGGWCCY 2 cut(s) 107, 232
Psp5II RGGWCCY 2 cut(s) 107, 232
Psp6I CCWGG 1 cut(s) 696
PspCI CACGTG 1 cut(s) 309
PspFI CCCAGC 2 cut(s) 34, 51
PspGI CCWGG 1 cut(s) 696
PspN4I GGNNCC 5 cut(s) 109, 163, 234, 538, 572
PspPI GGNCC 6 cut(s) 18, 107, 232, 312, 537, 571
PspPPI RGGWCCY 2 cut(s) 107, 232
PstNI CAGNNNCTG 1 cut(s) 298
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 8 cut(s) 64, 129, 151, 193, 304, 605, 608, 611
Sau3AI GATC 3 cut(s) 168, 445, 683
Sau96I GGNCC 6 cut(s) 18, 107, 232, 312, 537, 571
SchI GAGTC 3 cut(s) 240, 245, 406
ScrFI CCNGG 1 cut(s) 698
SfaNI GCATC 2 cut(s) 204, 363
SfoI GGCGCC 1 cut(s) 163
SinI GGWCC 5 cut(s) 107, 232, 312, 537, 571
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 2 cut(s) 451, 481
SspDI GGCGCC 1 cut(s) 161
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 696
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 541
TaiI ACGT 4 cut(s) 103, 279, 311, 555
TaqI TCGA 1 cut(s) 249
TaqII GACCGA 2 cut(s) 20, 634
TasI AATT 2 cut(s) 451, 481
TauI GCSGC 6 cut(s) 66, 131, 153, 306, 607, 610
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 2 cut(s) 192, 610
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 2 cut(s) 133, 391
TspGWI ACGGA 2 cut(s) 304, 316
VpaK11BI GGWCC 5 cut(s) 107, 232, 312, 537, 571
XapI RAATTY 1 cut(s) 481
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XmiI GTMKAC 1 cut(s) 330
XmnI GAANNNNTTC 2 cut(s) 398, 485
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.