RchiOBHm_Chr5g0048091

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001922
Physical Location & Seq
Reverse (-)
44682760 .. 44684194
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32591

Sequence Viewer

Length: 720 bp
ATGGCAGACCCTTCTCGGCCCGTCACCGGCTACCCAGCCCCGCCCTACGCCCAGCCCTACCCCGCCAACAACGGCCAGCCCAACGGCTCCTACCCCAACTACCCAGTCTACCAGCGTGGCCCCTACAACGCGTACACCGCACGCTCCGCCTTCGTCCGCCGCTTCGTCATCGCCATGGTCTTCCTCTTCGTCATCATCGGCTCCCTCCTCCTCATGATCTGGCTCATCCTCCGCCCCAAGATCCCCGACTTCCGAGTCGACTCGGTCACCGTCACCAACCTCAACGTCTCCTCCTCCCGCCAGTCCCTCACCGGCACCTGGTCCGTCGGCTACGACGTCTTCAACCCCAACAAGAAGATGACCATCGAGTACGACGAGATCGCCTCCTCCGTCTTCTACCGCTCCGGCTTCATTTCCGAGACCCGAATCCCGCCGTTCCGGCAGGGGAAGAGAGTCCGCAGCGCCGTGAACGCGACCTTCTCGGCGGCGAACTCGTTCGTCGACGACTGGGTGGCGAGAGGGATCAATGATGACAGGGCGGCGCGTGGGGCGGTGAGTTTCAATATCAAGCTGCTGGCTAGGGTTCAGTTCCGGAAGGGCGGGTGGAGGCTGAGGCGGCGGCTGCTAAGGGTGCTGTGCCGTGACGTGCCGGTTACGATTTCGAATAATGGGACCGGGACGATGACCGGCGGAGGTAGAGATTGCGGCGTTGCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

239

Amino Acids

26.63

Weight (kDa)

10.54

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
AatII GACGTC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 402
AccI GTMKAC 3 cut(s) 108, 258, 501
AccII CGCG 3 cut(s) 131, 473, 544
AccIII TCCGGA 1 cut(s) 591
AclWI GGATC 2 cut(s) 235, 530
AcoI YGGCCR 1 cut(s) 73
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 2 cut(s) 134, 371
AfiI CCNNNNNNNGG 2 cut(s) 26, 318
AflIII ACRYGT 1 cut(s) 129
AgsI TTSAA 2 cut(s) 343, 562
AjiI CACGTC 1 cut(s) 646
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 1 cut(s) 571
AluI AGCT 1 cut(s) 571
Alw26I GTCTC 2 cut(s) 292, 413
AlwI GGATC 2 cut(s) 235, 530
Aor13HI TCCGGA 1 cut(s) 591
AoxI GGCC 3 cut(s) 17, 73, 118
ApeKI GCWGC 3 cut(s) 459, 571, 622
Asp700I GAANNNNTTC 1 cut(s) 494
AspLEI GCGC 2 cut(s) 464, 544
AspS9I GGNCC 4 cut(s) 18, 119, 321, 672
AsuC2I CCSGG 1 cut(s) 676
AsuHPI GGTGA 5 cut(s) 16, 259, 265, 301, 565
AsuII TTCGAA 1 cut(s) 662
AvaII GGWCC 2 cut(s) 321, 672
BanI GGYRCC 1 cut(s) 314
BbsI GAAGAC 3 cut(s) 172, 331, 385
BbvCI CCTCAGC 1 cut(s) 611
BbvI GCAGC 3 cut(s) 471, 558, 609
BccI CCATC 1 cut(s) 371
BceAI ACGGC 5 cut(s) 88, 100, 418, 449, 624
BciT130I CCWGG 1 cut(s) 319
BcnI CCSGG 1 cut(s) 676
BcoDI GTCTC 2 cut(s) 292, 413
BfaI CTAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 465
BglI GCCNNNNNGGC 1 cut(s) 439
BisI GCNGC 9 cut(s) 160, 460, 486, 540, 572, 617, 620, 623, 706
BlsI GCNGC 9 cut(s) 161, 461, 487, 541, 573, 618, 621, 624, 707
Bme1390I CCNGG 2 cut(s) 319, 676
Bme18I GGWCC 2 cut(s) 321, 672
BmgBI CACGTC 1 cut(s) 646
BmgT120I GGNCC 4 cut(s) 18, 119, 321, 672
BmiI GGNNCC 5 cut(s) 88, 121, 202, 316, 673
BmrFI CCNGG 2 cut(s) 319, 676
BmrI ACTGGG 2 cut(s) 98, 517
BmuI ACTGGG 2 cut(s) 98, 517
BpiI GAAGAC 3 cut(s) 172, 331, 385
BplI GAGNNNNNCTC 2 cut(s) 368, 400
Bpu10I CCTNAGC 2 cut(s) 611, 626
Bpu14I TTCGAA 1 cut(s) 662
BpuMI CCSGG 1 cut(s) 676
BsaBI GATNNNNATC 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 174
BsaWI WCCGGW 1 cut(s) 591
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 318
Bse118I RCCGGY 4 cut(s) 26, 311, 649, 686
Bse1I ACTGG 3 cut(s) 104, 301, 512
Bse8I GATNNNNATC 1 cut(s) 362
BseAI TCCGGA 1 cut(s) 591
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 1 cut(s) 225
BseJI GATNNNNATC 1 cut(s) 362
BseLI CCNNNNNNNGG 2 cut(s) 26, 318
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 3 cut(s) 104, 301, 512
BseRI GAGGAG 5 cut(s) 197, 200, 280, 283, 376
BseXI GCAGC 3 cut(s) 471, 558, 609
BseYI CCCAGC 2 cut(s) 34, 51
Bsh1236I CGCG 3 cut(s) 131, 473, 544
BshFI GGCC 3 cut(s) 19, 75, 120
BshNI GGYRCC 1 cut(s) 314
BsiSI CCGG 8 cut(s) 27, 312, 405, 439, 592, 650, 675, 687
BslFI GGGAC 3 cut(s) 289, 685, 691
BslI CCNNNNNNNGG 2 cut(s) 26, 318
BsmAI GTCTC 2 cut(s) 292, 413
BsmBI CGTCTC 1 cut(s) 292
BsmFI GGGAC 3 cut(s) 289, 685, 691
BsnI GGCC 3 cut(s) 19, 75, 120
Bso31I GGTCTC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 662
Bsp13I TCCGGA 1 cut(s) 591
Bsp143I GATC 4 cut(s) 216, 240, 378, 522
Bsp19I CCATGG 1 cut(s) 174
BspANI GGCC 3 cut(s) 19, 75, 120
BspCNI CTCAG 1 cut(s) 603
BspEI TCCGGA 1 cut(s) 591
BspFNI CGCG 3 cut(s) 131, 473, 544
BspHI TCATGA 1 cut(s) 213
BspLI GGNNCC 5 cut(s) 88, 121, 202, 316, 673
BspPI GGATC 2 cut(s) 235, 530
BspT104I TTCGAA 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 314
BspTNI GGTCTC 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 402
BsrFI RCCGGY 4 cut(s) 26, 311, 649, 686
BsrI ACTGG 3 cut(s) 104, 301, 512
BssAI RCCGGY 4 cut(s) 26, 311, 649, 686
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 4 cut(s) 216, 240, 378, 522
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 2 cut(s) 191, 443
BstACI GRCGYC 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 662
BstC8I GCNNGC 3 cut(s) 77, 142, 576
BstDEI CTNAG 2 cut(s) 611, 626
BstDSI CCRYGG 1 cut(s) 174
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 1 cut(s) 225
BstFNI CGCG 3 cut(s) 131, 473, 544
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 2 cut(s) 464, 544
BstKTI GATC 4 cut(s) 219, 243, 381, 525
BstMAI GTCTC 2 cut(s) 292, 413
BstMBI GATC 4 cut(s) 216, 240, 378, 522
BstMWI GCNNNNNNNGC 8 cut(s) 137, 146, 439, 470, 548, 616, 622, 631
BstNI CCWGG 1 cut(s) 319
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 317, 674
BstUI CGCG 3 cut(s) 131, 473, 544
BstV1I GCAGC 3 cut(s) 471, 558, 609
BstV2I GAAGAC 3 cut(s) 172, 331, 385
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
BsuRI GGCC 3 cut(s) 19, 75, 120
BtgI CCRYGG 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 154
BtrI CACGTC 1 cut(s) 646
BtsCI GGATG 1 cut(s) 225
Cac8I GCNNGC 3 cut(s) 77, 142, 576
CciI TCATGA 1 cut(s) 213
CfoI GCGC 2 cut(s) 464, 544
Cfr10I RCCGGY 4 cut(s) 26, 311, 649, 686
Cfr13I GGNCC 4 cut(s) 18, 119, 321, 672
CsiI ACCWGGT 1 cut(s) 317
Csp6I GTAC 2 cut(s) 133, 370
CviAII CATG 2 cut(s) 175, 214
CviQI GTAC 2 cut(s) 133, 370
DdeI CTNAG 2 cut(s) 611, 626
DpnI GATC 4 cut(s) 218, 242, 380, 524
DpnII GATC 4 cut(s) 216, 240, 378, 522
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 2 cut(s) 191, 443
EarI CTCTTC 2 cut(s) 191, 443
EciI GGCGGA 4 cut(s) 136, 146, 221, 705
Eco130I CCWWGG 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 413
Eco47I GGWCC 2 cut(s) 321, 672
Eco91I GGTNACC 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 2 cut(s) 178, 217
FaiI YATR 2 cut(s) 176, 215
FaqI GGGAC 3 cut(s) 289, 685, 691
FatI CATG 2 cut(s) 174, 213
FauI CCCGC 5 cut(s) 48, 70, 305, 438, 593
FblI GTMKAC 3 cut(s) 108, 258, 501
Fnu4HI GCNGC 9 cut(s) 160, 460, 486, 540, 572, 617, 620, 623, 706
FokI GGATG 1 cut(s) 212
Fsp4HI GCNGC 9 cut(s) 160, 460, 486, 540, 572, 617, 620, 623, 706
FspBI CTAG 1 cut(s) 579
GlaI GCGC 2 cut(s) 463, 543
GluI GCNGC 9 cut(s) 160, 460, 486, 540, 572, 617, 620, 623, 706
GsaI CCCAGC 2 cut(s) 38, 55
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 3 cut(s) 19, 75, 120
HapII CCGG 8 cut(s) 27, 312, 405, 439, 592, 650, 675, 687
HhaI GCGC 2 cut(s) 464, 544
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 2 cut(s) 178, 217
Hin6I GCGC 2 cut(s) 462, 542
HinP1I GCGC 2 cut(s) 462, 542
HincII GTYRAC 2 cut(s) 259, 502
HindII GTYRAC 2 cut(s) 259, 502
HinfI GANTC 4 cut(s) 255, 260, 426, 453
HpaII CCGG 8 cut(s) 27, 312, 405, 439, 592, 650, 675, 687
HphI GGTGA 5 cut(s) 16, 259, 265, 301, 565
Hpy166II GTNNAC 5 cut(s) 109, 135, 259, 469, 502
Hpy188I TCNGA 3 cut(s) 254, 418, 719
Hpy188III TCNNGA 2 cut(s) 214, 592
Hpy8I GTNNAC 5 cut(s) 109, 135, 259, 469, 502
Hpy99I CGWCG 5 cut(s) 329, 338, 377, 503, 506
HpyAV CCTTC 4 cut(s) 21, 160, 487, 589
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 3 cut(s) 285, 336, 645
HpyF10VI GCNNNNNNNGC 8 cut(s) 137, 146, 439, 470, 548, 616, 622, 631
HpyF3I CTNAG 2 cut(s) 611, 626
HpySE526I ACGT 3 cut(s) 285, 336, 645
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 2 cut(s) 178, 217
HspAI GCGC 2 cut(s) 462, 542
Kpn2I TCCGGA 1 cut(s) 591
Kzo9I GATC 4 cut(s) 216, 240, 378, 522
LmnI GCTCC 4 cut(s) 92, 149, 206, 407
Lsp1109I GCAGC 3 cut(s) 471, 558, 609
MabI ACCWGGT 1 cut(s) 317
MaeI CTAG 1 cut(s) 579
MaeII ACGT 3 cut(s) 285, 336, 645
MaeIII GTNAC 5 cut(s) 22, 265, 271, 641, 652
MalI GATC 4 cut(s) 218, 242, 380, 524
MbiI CCGCTC 1 cut(s) 402
MboI GATC 4 cut(s) 216, 240, 378, 522
MboII GAAGA 6 cut(s) 172, 178, 331, 367, 385, 460
MflI RGATCY 1 cut(s) 240
MluI ACGCGT 1 cut(s) 129
MlyI GAGTC 3 cut(s) 254, 264, 462
MroI TCCGGA 1 cut(s) 591
MroXI GAANNNNTTC 1 cut(s) 494
MslI CAYNNNNRTG 1 cut(s) 173
MspI CCGG 8 cut(s) 27, 312, 405, 439, 592, 650, 675, 687
MspR9I CCNGG 2 cut(s) 319, 676
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 3 cut(s) 131, 473, 544
MwoI GCNNNNNNNGC 8 cut(s) 137, 146, 439, 470, 548, 616, 622, 631
NciI CCSGG 1 cut(s) 676
NcoI CCATGG 1 cut(s) 174
NdeII GATC 4 cut(s) 216, 240, 378, 522
NlaIII CATG 2 cut(s) 178, 217
NlaIV GGNNCC 5 cut(s) 88, 121, 202, 316, 673
NmeAIII GCCGAG 1 cut(s) 461
NmuCI GTSAC 4 cut(s) 22, 265, 271, 641
NspV TTCGAA 1 cut(s) 662
PagI TCATGA 1 cut(s) 213
PcsI WCGNNNNNNNCGW 3 cut(s) 333, 372, 387
PdmI GAANNNNTTC 1 cut(s) 494
PfeI GAWTC 1 cut(s) 426
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 9 cut(s) 161, 461, 487, 541, 573, 618, 621, 624, 707
PleI GAGTC 3 cut(s) 254, 263, 461
PpsI GAGTC 3 cut(s) 254, 263, 461
Psp6I CCWGG 1 cut(s) 317
PspEI GGTNACC 1 cut(s) 265
PspFI CCCAGC 2 cut(s) 34, 51
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 5 cut(s) 88, 121, 202, 316, 673
PspPI GGNCC 4 cut(s) 18, 119, 321, 672
PsuI RGATCY 1 cut(s) 240
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 2 cut(s) 134, 371
RsaNI GTAC 2 cut(s) 133, 370
RseI CAYNNNNRTG 1 cut(s) 173
SalI GTCGAC 2 cut(s) 257, 500
SatI GCNGC 9 cut(s) 160, 460, 486, 540, 572, 617, 620, 623, 706
Sau3AI GATC 4 cut(s) 216, 240, 378, 522
Sau96I GGNCC 4 cut(s) 18, 119, 321, 672
SchI GAGTC 3 cut(s) 254, 264, 462
ScrFI CCNGG 2 cut(s) 319, 676
SetI ASST 8 cut(s) 282, 288, 320, 339, 479, 573, 648, 697
SexAI ACCWGGT 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 662
SgrDI CGTCGACG 1 cut(s) 500
SinI GGWCC 2 cut(s) 321, 672
SmiMI CAYNNNNRTG 1 cut(s) 173
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 2 cut(s) 317, 674
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 3 cut(s) 288, 339, 648
TaqI TCGA 4 cut(s) 258, 366, 501, 662
TaqII GACCGA 1 cut(s) 253
TauI GCSGC 6 cut(s) 162, 488, 542, 619, 622, 708
TfiI GAWTC 1 cut(s) 426
TseFI GTSAC 4 cut(s) 22, 265, 271, 641
TseI GCWGC 3 cut(s) 459, 571, 622
Tsp45I GTSAC 4 cut(s) 22, 265, 271, 641
TspDTI ATGAA 1 cut(s) 400
TspGWI ACGGA 2 cut(s) 313, 379
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 321, 672
XmiI GTMKAC 3 cut(s) 108, 258, 501
XmnI GAANNNNTTC 1 cut(s) 494
XspI CTAG 1 cut(s) 579
ZraI GACGTC 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.