MD03G1153500.v1.1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001922
Physical Location & Seq
Reverse (-)
17395004 .. 17395714
711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1153500.v1.1.491

Sequence Viewer

Length: 711 bp
ATGACCGACCCTTCGCGGCCCGTCACCGGCTACCCAGCCCCGCCCTACGCCCAGCCCAATGGCGGCCAACCTTACTCCAGCTACCCCACCCACACCCAACCTCAAGGCCCCTACTCCAACGCCTACGCCGCACGCGCTACCTTCATCCGCCGCTTCGTCGGCGCCATGGTCGCCCTCTTCGTCATCGTCGGCTGCATCATGCTCGTGATCTGGCTCGTCCTCCGCCCCAAGCTCCCCGACTTCCGAGTCGACTCTCTCTCACTCACCAACTTCAACGTCTCCTCCTCCTCGCAGTCCTTGACCGGCACGTGGTCCGTCGGCTTCTCCGTCTACAACCCCAACAAGAAGCTCAGTATCCGATACGAGGAGGTCGAGTCCTCCATTTTTTACGGGAAGGGCCTCATATCGGACGCTCGTTCCCAGCCGTTCGCTCAGGGGACGAAGGATCGCAATTTCATCAACGCCACCTTCTCGGCGGCGAATTCGTTCGTGGACGGGCCGGTGGTGCGCGGAATCGAGACGGACAGGGAGCGCGGGACCGTGAGCTTCAACATCAAGGTTTGGGCTAGGGTTGAGTTTCGGAATGGCGGGTGGAGGCTGAGGCGGCGGCTGCTTAGGGTTTTCTGCCGTGACGTGGCGGTTTCGGTTTCTTCCAATAGCGGGTCGGGTAAGCTCGCAGGTGGATCAAGAGATTGTCAGGTGTATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

237

Amino Acids

26.07

Weight (kDa)

10.05

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 108 - 209 1e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 668
AasI GACNNNNNNGTC 1 cut(s) 245
Acc36I ACCTGC 1 cut(s) 668
AccB1I GGYRCC 1 cut(s) 161
AccI GTMKAC 2 cut(s) 249, 330
AccII CGCG 4 cut(s) 16, 135, 510, 534
AclWI GGATC 2 cut(s) 453, 691
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 1 cut(s) 481
AcvI CACGTG 1 cut(s) 309
AcyI GRCGYC 1 cut(s) 162
AfiI CCNNNNNNNGG 7 cut(s) 26, 62, 309, 364, 406, 634, 660
AgsI TTSAA 2 cut(s) 274, 550
AjiI CACGTC 1 cut(s) 634
AluBI AGCT 5 cut(s) 81, 232, 349, 546, 673
AluI AGCT 5 cut(s) 81, 232, 349, 546, 673
Alw26I GTCTC 2 cut(s) 283, 512
AlwI GGATC 2 cut(s) 453, 691
AoxI GGCC 5 cut(s) 17, 64, 106, 397, 497
ApeKI GCWGC 2 cut(s) 192, 610
ApoI RAATTY 1 cut(s) 481
ArsI GACNNNNNNTTYG 1 cut(s) 27
Asp700I GAANNNNTTC 1 cut(s) 485
AspLEI GCGC 4 cut(s) 137, 164, 510, 534
AspS9I GGNCC 6 cut(s) 18, 107, 312, 397, 497, 537
AsuHPI GGTGA 2 cut(s) 16, 256
AvaII GGWCC 2 cut(s) 312, 537
BanI GGYRCC 1 cut(s) 161
BauI CACGAG 1 cut(s) 203
BbrPI CACGTG 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 599
BbvI GCAGC 2 cut(s) 179, 597
BceAI ACGGC 2 cut(s) 409, 612
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciVI GTATCC 1 cut(s) 365
BcoDI GTCTC 2 cut(s) 283, 512
BfaI CTAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 668
BfuI GTATCC 1 cut(s) 365
BisI GCNGC 9 cut(s) 17, 64, 129, 151, 193, 477, 605, 608, 611
BlsI GCNGC 9 cut(s) 18, 65, 130, 152, 194, 478, 606, 609, 612
Bme18I GGWCC 2 cut(s) 312, 537
BmgBI CACGTC 1 cut(s) 634
BmgT120I GGNCC 6 cut(s) 18, 107, 312, 397, 497, 537
BmiI GGNNCC 3 cut(s) 109, 163, 538
BmsI GCATC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 61
Bpu10I CCTNAGC 3 cut(s) 432, 599, 614
BpuEI CTTGAG 1 cut(s) 87
BsaAI YACGTR 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 359, 389
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 62, 309, 364, 406, 634, 660
Bse118I RCCGGY 3 cut(s) 26, 302, 499
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 7 cut(s) 26, 62, 309, 364, 406, 634, 660
BseMII CTCAG 3 cut(s) 364, 446, 590
BseRI GAGGAG 4 cut(s) 271, 274, 277, 380
BseXI GCAGC 2 cut(s) 179, 597
BseYI CCCAGC 3 cut(s) 34, 51, 420
Bsh1236I CGCG 4 cut(s) 16, 135, 510, 534
BshFI GGCC 5 cut(s) 19, 66, 108, 399, 499
BshNI GGYRCC 1 cut(s) 161
BsiSI CCGG 3 cut(s) 27, 303, 500
BslFI GGGAC 2 cut(s) 451, 550
BslI CCNNNNNNNGG 7 cut(s) 26, 62, 309, 364, 406, 634, 660
BsmAI GTCTC 2 cut(s) 283, 512
BsmBI CGTCTC 2 cut(s) 283, 512
BsmFI GGGAC 2 cut(s) 451, 550
BsnI GGCC 5 cut(s) 19, 66, 108, 399, 499
Bsp143I GATC 3 cut(s) 207, 445, 683
Bsp19I CCATGG 1 cut(s) 165
BspANI GGCC 5 cut(s) 19, 66, 108, 399, 499
BspCNI CTCAG 3 cut(s) 363, 445, 591
BspFNI CGCG 4 cut(s) 16, 135, 510, 534
BspLI GGNNCC 3 cut(s) 109, 163, 538
BspMI ACCTGC 1 cut(s) 668
BspPI GGATC 2 cut(s) 453, 691
BspT107I GGYRCC 1 cut(s) 161
BsrFI RCCGGY 3 cut(s) 26, 302, 499
BssAI RCCGGY 3 cut(s) 26, 302, 499
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 3 cut(s) 207, 445, 683
BssNI GRCGYC 1 cut(s) 162
BssSI CACGAG 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 541
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 162
BstBAI YACGTR 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 133, 675
BstDEI CTNAG 4 cut(s) 350, 432, 599, 614
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 1 cut(s) 144
BstFNI CGCG 4 cut(s) 16, 135, 510, 534
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 137, 164, 510, 534
BstKTI GATC 3 cut(s) 210, 448, 686
BstMAI GTCTC 2 cut(s) 283, 512
BstMBI GATC 3 cut(s) 207, 445, 683
BstMWI GCNNNNNNNGC 7 cut(s) 128, 134, 159, 170, 505, 604, 610
BstUI CGCG 4 cut(s) 16, 135, 510, 534
BstV1I GCAGC 2 cut(s) 179, 597
BstXI CCANNNNNNTGG 1 cut(s) 59
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 5 cut(s) 19, 66, 108, 399, 499
BtgI CCRYGG 1 cut(s) 165
BtrI CACGTC 1 cut(s) 634
BtsCI GGATG 1 cut(s) 144
BveI ACCTGC 1 cut(s) 668
Cac8I GCNNGC 2 cut(s) 133, 675
CfoI GCGC 4 cut(s) 137, 164, 510, 534
Cfr10I RCCGGY 3 cut(s) 26, 302, 499
Cfr13I GGNCC 6 cut(s) 18, 107, 312, 397, 497, 537
CseI GACGC 1 cut(s) 419
CviAII CATG 2 cut(s) 166, 199
DdeI CTNAG 4 cut(s) 350, 432, 599, 614
DinI GGCGCC 1 cut(s) 163
DpnI GATC 3 cut(s) 209, 447, 685
DpnII GATC 3 cut(s) 207, 445, 683
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
EciI GGCGGA 2 cut(s) 137, 212
Eco130I CCWWGG 1 cut(s) 165
Eco47I GGWCC 2 cut(s) 312, 537
Eco72I CACGTG 1 cut(s) 309
EcoO109I RGGNCCY 2 cut(s) 107, 397
EcoRI GAATTC 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 165
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 2 cut(s) 283, 512
FaeI CATG 2 cut(s) 169, 202
FaiI YATR 4 cut(s) 167, 200, 404, 705
FaqI GGGAC 2 cut(s) 451, 550
FatI CATG 2 cut(s) 165, 198
FauI CCCGC 4 cut(s) 48, 527, 581, 653
FblI GTMKAC 2 cut(s) 249, 330
Fnu4HI GCNGC 9 cut(s) 17, 64, 129, 151, 193, 477, 605, 608, 611
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 9 cut(s) 17, 64, 129, 151, 193, 477, 605, 608, 611
FspBI CTAG 1 cut(s) 567
GlaI GCGC 4 cut(s) 136, 163, 509, 533
GluI GCNGC 9 cut(s) 17, 64, 129, 151, 193, 477, 605, 608, 611
GsaI CCCAGC 3 cut(s) 38, 55, 424
GsuI CTGGAG 1 cut(s) 61
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 5 cut(s) 19, 66, 108, 399, 499
HapII CCGG 3 cut(s) 27, 303, 500
HgaI GACGC 1 cut(s) 419
HhaI GCGC 4 cut(s) 137, 164, 510, 534
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 2 cut(s) 169, 202
Hin6I GCGC 4 cut(s) 135, 162, 508, 532
HinP1I GCGC 4 cut(s) 135, 162, 508, 532
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 4 cut(s) 246, 251, 374, 513
HpaII CCGG 3 cut(s) 27, 303, 500
HphI GGTGA 2 cut(s) 16, 256
Hpy166II GTNNAC 3 cut(s) 250, 331, 493
Hpy188I TCNGA 4 cut(s) 245, 359, 409, 582
Hpy188III TCNNGA 3 cut(s) 205, 517, 687
Hpy8I GTNNAC 3 cut(s) 250, 331, 493
Hpy99I CGWCG 3 cut(s) 161, 191, 320
HpyAV CCTTC 5 cut(s) 21, 151, 388, 436, 478
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4IV ACGT 3 cut(s) 276, 308, 633
HpyCH4V TGCA 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 134, 159, 170, 505, 604, 610
HpyF3I CTNAG 4 cut(s) 350, 432, 599, 614
HpySE526I ACGT 3 cut(s) 276, 308, 633
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 2 cut(s) 169, 202
HspAI GCGC 4 cut(s) 135, 162, 508, 532
KasI GGCGCC 1 cut(s) 161
Kzo9I GATC 3 cut(s) 207, 445, 683
LmnI GCTCC 2 cut(s) 237, 529
Lsp1109I GCAGC 2 cut(s) 179, 597
LweI GCATC 1 cut(s) 204
MaeI CTAG 1 cut(s) 567
MaeII ACGT 3 cut(s) 276, 308, 633
MaeIII GTNAC 2 cut(s) 22, 629
MalI GATC 3 cut(s) 209, 447, 685
MboI GATC 3 cut(s) 207, 445, 683
MboII GAAGA 2 cut(s) 169, 642
MluCI AATT 2 cut(s) 451, 481
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 3 cut(s) 245, 255, 383
MmeI TCCRAC 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 485
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 3 cut(s) 27, 303, 500
MvnI CGCG 4 cut(s) 16, 135, 510, 534
MwoI GCNNNNNNNGC 7 cut(s) 128, 134, 159, 170, 505, 604, 610
NarI GGCGCC 1 cut(s) 162
NcoI CCATGG 1 cut(s) 165
NdeII GATC 3 cut(s) 207, 445, 683
NlaIII CATG 2 cut(s) 169, 202
NlaIV GGNNCC 3 cut(s) 109, 163, 538
NmeAIII GCCGAG 1 cut(s) 452
NmuCI GTSAC 2 cut(s) 22, 629
PaqCI CACCTGC 1 cut(s) 668
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 324, 369
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 1 cut(s) 513
PkrI GCNGC 9 cut(s) 18, 65, 130, 152, 194, 478, 606, 609, 612
PleI GAGTC 3 cut(s) 245, 254, 382
PluTI GGCGCC 1 cut(s) 165
PmaCI CACGTG 1 cut(s) 309
PmlI CACGTG 1 cut(s) 309
PpsI GAGTC 3 cut(s) 245, 254, 382
Ppu21I YACGTR 1 cut(s) 309
PspCI CACGTG 1 cut(s) 309
PspFI CCCAGC 3 cut(s) 34, 51, 420
PspN4I GGNNCC 3 cut(s) 109, 163, 538
PspPI GGNCC 6 cut(s) 18, 107, 312, 397, 497, 537
RseI CAYNNNNRTG 1 cut(s) 203
SalI GTCGAC 1 cut(s) 248
SatI GCNGC 9 cut(s) 17, 64, 129, 151, 193, 477, 605, 608, 611
Sau3AI GATC 3 cut(s) 207, 445, 683
Sau96I GGNCC 6 cut(s) 18, 107, 312, 397, 497, 537
SchI GAGTC 3 cut(s) 245, 255, 383
SfaNI GCATC 1 cut(s) 204
SfoI GGCGCC 1 cut(s) 163
SinI GGWCC 2 cut(s) 312, 537
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 2 cut(s) 451, 481
SspDI GGCGCC 1 cut(s) 161
SspMI CTAG 1 cut(s) 567
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 541
TaiI ACGT 3 cut(s) 279, 311, 636
TaqI TCGA 3 cut(s) 249, 372, 516
TaqII GACCGA 1 cut(s) 20
TasI AATT 2 cut(s) 451, 481
TauI GCSGC 7 cut(s) 19, 66, 131, 153, 479, 607, 610
TfiI GAWTC 1 cut(s) 513
TseFI GTSAC 2 cut(s) 22, 629
TseI GCWGC 2 cut(s) 192, 610
Tsp45I GTSAC 2 cut(s) 22, 629
TspDTI ATGAA 2 cut(s) 133, 445
TspGWI ACGGA 3 cut(s) 304, 316, 536
VpaK11BI GGWCC 2 cut(s) 312, 537
XapI RAATTY 1 cut(s) 481
XmiI GTMKAC 2 cut(s) 249, 330
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.