FvH4_4g00740

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002605
Physical Location & Seq
Forward (+)
652580 .. 654205
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00740.t1

Sequence Viewer

Length: 717 bp
ATGGCTAACATGAGCTGTATTACAGCGGTGGGCGGGCAGAGAAGCTCAAAATTGGGGTACTCTAGCAGCTCAACCGACAGCAACACTGATGACACAAAGAAGGTTGGCGCTGAAGAAGATATTGATGATTTTCTTGGTGGTCTGAAAGATGTGGACCTGGCAGCCCAAGGTGTGGATCCCAAGAGGGGTTGGGGATTTCGCGGTGTGCACAAGGGGTACCAGAAACTTGTTCTAGATATTACTGGACACAACCTCTTGGTTCCATTGGGATGCAATTATTTGTGGAAGAGTTGGGCAATCCCCCGTGCAGAAGATCTTGAGGAATGGTCGAACTGTAACCATCTTCACTGTTGGGACCGGGGCAAGTTTGACCAGAGATTACTGCGAAAGGACTTGCCAAAACCAGCTCAGTGGCACCGCATTGCTGTACATAATGACAGACTTGGGGCTTATGCAGTTAAACAATTTGTTAAGAATTCATCAGTTTATGTTGAGGGTGACATCGAGACTAGAGTTTACAACGACAGCATCACTGGTGAAGTTAATAATATTCCGGAGATCTGCATTCGTCACGATGGGAAGATCCGTCTTATAAAGAGTGGAGAAAGCATCAGCAGTATATCCTTGGATGACCTGCGGGAACCTCAGCCCCGGAACGGGAAAGCATCTCAAGCCACTTCTGTAGGAAACATGGATATGTTTCCAAGTTGGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SSB

Protein Analysis

239

Amino Acids

26.57

Weight (kDa)

6.38

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 133 - 190 7e-08 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 593
Acc36I ACCTGC 1 cut(s) 642
Acc65I GGTACC 1 cut(s) 216
AccB1I GGYRCC 2 cut(s) 216, 414
AccB7I CCANNNNNTGG 1 cut(s) 172
AccII CGCG 1 cut(s) 201
AccIII TCCGGA 1 cut(s) 553
AciI CCGC 5 cut(s) 26, 33, 201, 418, 637
AclWI GGATC 3 cut(s) 170, 183, 577
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 3 cut(s) 59, 218, 429
AfiI CCNNNNNNNGG 4 cut(s) 172, 185, 656, 657
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 4 cut(s) 15, 45, 69, 407
AluI AGCT 4 cut(s) 15, 45, 69, 407
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 500
Alw44I GTGCAC 1 cut(s) 206
AlwI GGATC 3 cut(s) 170, 183, 577
Aor13HI TCCGGA 1 cut(s) 553
ApaLI GTGCAC 1 cut(s) 206
ApeKI GCWGC 2 cut(s) 66, 161
ApoI RAATTY 1 cut(s) 475
Asp718I GGTACC 1 cut(s) 216
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 2 cut(s) 154, 355
AsuC2I CCSGG 2 cut(s) 359, 652
AsuHPI GGTGA 2 cut(s) 509, 548
AvaII GGWCC 2 cut(s) 154, 355
BaeGI GKGCMC 1 cut(s) 210
BamHI GGATCC 1 cut(s) 175
BanI GGYRCC 2 cut(s) 216, 414
Bbv12I GWGCWC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 645
BbvI GCAGC 2 cut(s) 78, 173
BccI CCATC 2 cut(s) 348, 569
BciT130I CCWGG 1 cut(s) 158
BcnI CCSGG 2 cut(s) 359, 652
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 3 cut(s) 63, 233, 510
BfmI CTRYAG 1 cut(s) 681
BfoI RGCGCY 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 642
BglII AGATCT 2 cut(s) 313, 558
BisI GCNGC 2 cut(s) 67, 162
BlsI GCNGC 2 cut(s) 68, 163
Bme1390I CCNGG 3 cut(s) 158, 359, 652
Bme18I GGWCC 2 cut(s) 154, 355
BmgT120I GGNCC 2 cut(s) 154, 355
BmiI GGNNCC 6 cut(s) 177, 218, 261, 356, 416, 642
BmrFI CCNGG 3 cut(s) 158, 359, 652
BmsI GCATC 4 cut(s) 260, 537, 618, 674
Bpu10I CCTNAGC 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 338, 654
BpuMI CCSGG 2 cut(s) 359, 652
BsaJI CCNNGG 4 cut(s) 166, 358, 624, 650
BsaWI WCCGGW 1 cut(s) 553
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 185, 656, 657
Bse1I ACTGG 2 cut(s) 247, 538
Bse3DI GCAATG 1 cut(s) 420
BseAI TCCGGA 1 cut(s) 553
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 4 cut(s) 166, 358, 624, 650
BseGI GGATG 2 cut(s) 275, 634
BseLI CCNNNNNNNGG 4 cut(s) 172, 185, 656, 657
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 422, 659
BseNI ACTGG 2 cut(s) 247, 538
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 2 cut(s) 78, 173
BsgI GTGCAG 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 201
BshNI GGYRCC 2 cut(s) 216, 414
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 3 cut(s) 358, 554, 652
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 4 cut(s) 172, 185, 656, 657
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 368
BsmI GAATGC 1 cut(s) 564
Bsp1286I GDGCHC 1 cut(s) 210
Bsp13I TCCGGA 1 cut(s) 553
Bsp1407I TGTACA 1 cut(s) 427
Bsp143I GATC 4 cut(s) 175, 313, 558, 582
BspACI CCGC 5 cut(s) 26, 33, 201, 418, 637
BspCNI CTCAG 2 cut(s) 421, 658
BspEI TCCGGA 1 cut(s) 553
BspFNI CGCG 1 cut(s) 201
BspLI GGNNCC 6 cut(s) 177, 218, 261, 356, 416, 642
BspMI ACCTGC 1 cut(s) 642
BspPI GGATC 3 cut(s) 170, 183, 577
BspT107I GGYRCC 2 cut(s) 216, 414
BsrDI GCAATG 1 cut(s) 420
BsrGI TGTACA 1 cut(s) 427
BsrI ACTGG 2 cut(s) 247, 538
BssECI CCNNGG 4 cut(s) 166, 358, 624, 650
BssMI GATC 4 cut(s) 175, 313, 558, 582
BssT1I CCWWGG 2 cut(s) 166, 624
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 2 cut(s) 335, 350
Bst6I CTCTTC 1 cut(s) 281
BstAUI TGTACA 1 cut(s) 427
BstC8I GCNNGC 1 cut(s) 35
BstDEI CTNAG 2 cut(s) 408, 645
BstF5I GGATG 2 cut(s) 275, 634
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 4 cut(s) 178, 316, 561, 585
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 4 cut(s) 175, 313, 558, 582
BstMWI GCNNNNNNNGC 1 cut(s) 671
BstNI CCWGG 1 cut(s) 158
BstSCI CCNGG 3 cut(s) 156, 357, 650
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 1 cut(s) 210
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 2 cut(s) 78, 173
BstX2I RGATCY 4 cut(s) 175, 313, 558, 582
BstXI CCANNNNNNTGG 1 cut(s) 411
BstYI RGATCY 4 cut(s) 175, 313, 558, 582
BtsCI GGATG 2 cut(s) 275, 634
BtsIMutI CAGTG 4 cut(s) 84, 346, 416, 531
BveI ACCTGC 1 cut(s) 642
Cac8I GCNNGC 1 cut(s) 35
CfoI GCGC 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 154, 355
Csp6I GTAC 3 cut(s) 58, 217, 428
CviAII CATG 2 cut(s) 10, 691
CviJI RGCY 9 cut(s) 5, 15, 45, 69, 164, 407, 449, 649, 674
CviKI_1 RGCY 9 cut(s) 5, 15, 45, 69, 164, 407, 449, 649, 674
CviQI GTAC 3 cut(s) 58, 217, 428
DdeI CTNAG 2 cut(s) 408, 645
DpnI GATC 4 cut(s) 177, 315, 560, 584
DpnII GATC 4 cut(s) 175, 313, 558, 582
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 166, 624
Eco47I GGWCC 2 cut(s) 154, 355
Eco57I CTGAAG 1 cut(s) 132
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 156
EcoT14I CCWWGG 2 cut(s) 166, 624
ErhI CCWWGG 2 cut(s) 166, 624
FaeI CATG 2 cut(s) 13, 694
FaiI YATR 8 cut(s) 11, 432, 453, 489, 593, 620, 692, 698
FaqI GGGAC 1 cut(s) 368
FatI CATG 2 cut(s) 9, 690
FauI CCCGC 2 cut(s) 26, 630
Fnu4HI GCNGC 2 cut(s) 67, 162
FokI GGATG 2 cut(s) 282, 641
Fsp4HI GCNGC 2 cut(s) 67, 162
FspBI CTAG 3 cut(s) 63, 233, 510
GlaI GCGC 1 cut(s) 109
GluI GCNGC 2 cut(s) 67, 162
HaeII RGCGCY 1 cut(s) 111
HapII CCGG 3 cut(s) 358, 554, 652
HhaI GCGC 1 cut(s) 110
Hin1II CATG 2 cut(s) 13, 694
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HpaII CCGG 3 cut(s) 358, 554, 652
HphI GGTGA 2 cut(s) 509, 548
Hpy166II GTNNAC 3 cut(s) 154, 208, 517
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 5 cut(s) 233, 317, 505, 554, 572
Hpy8I GTNNAC 3 cut(s) 154, 208, 517
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 335, 350
HpyCH4V TGCA 5 cut(s) 208, 273, 308, 455, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 671
HpyF3I CTNAG 2 cut(s) 408, 645
Hsp92II CATG 2 cut(s) 13, 694
HspAI GCGC 1 cut(s) 108
Kpn2I TCCGGA 1 cut(s) 553
KpnI GGTACC 1 cut(s) 220
Kzo9I GATC 4 cut(s) 175, 313, 558, 582
Lsp1109I GCAGC 2 cut(s) 78, 173
LweI GCATC 4 cut(s) 260, 537, 618, 674
MaeI CTAG 3 cut(s) 63, 233, 510
MaeIII GTNAC 3 cut(s) 335, 497, 569
MalI GATC 4 cut(s) 177, 315, 560, 584
MboI GATC 4 cut(s) 175, 313, 558, 582
MboII GAAGA 6 cut(s) 125, 128, 298, 323, 335, 592
MflI RGATCY 4 cut(s) 175, 313, 558, 582
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 4 cut(s) 50, 274, 464, 475
MnlI CCTC 5 cut(s) 177, 263, 313, 487, 654
MroI TCCGGA 1 cut(s) 553
MseI TTAA 3 cut(s) 459, 471, 543
MslI CAYNNNNRTG 2 cut(s) 268, 695
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 3 cut(s) 358, 554, 652
MspR9I CCNGG 3 cut(s) 158, 359, 652
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 1 cut(s) 158
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 1 cut(s) 671
NciI CCSGG 2 cut(s) 359, 652
NdeII GATC 4 cut(s) 175, 313, 558, 582
NlaIII CATG 2 cut(s) 13, 694
NlaIV GGNNCC 6 cut(s) 177, 218, 261, 356, 416, 642
NmuCI GTSAC 2 cut(s) 497, 569
PctI GAATGC 1 cut(s) 564
PflMI CCANNNNNTGG 1 cut(s) 172
PkrI GCNGC 2 cut(s) 68, 163
PsiI TTATAA 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 6 cut(s) 177, 218, 261, 356, 416, 642
PspPI GGNCC 2 cut(s) 154, 355
PsuI RGATCY 4 cut(s) 175, 313, 558, 582
RsaI GTAC 3 cut(s) 59, 218, 429
RsaNI GTAC 3 cut(s) 58, 217, 428
RseI CAYNNNNRTG 2 cut(s) 268, 695
SaqAI TTAA 3 cut(s) 459, 471, 543
SatI GCNGC 2 cut(s) 67, 162
Sau3AI GATC 4 cut(s) 175, 313, 558, 582
Sau96I GGNCC 2 cut(s) 154, 355
ScrFI CCNGG 3 cut(s) 158, 359, 652
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 4 cut(s) 260, 537, 618, 674
SfcI CTRYAG 1 cut(s) 681
SinI GGWCC 2 cut(s) 154, 355
SmiMI CAYNNNNRTG 2 cut(s) 268, 695
SmlI CTYRAG 2 cut(s) 317, 669
SmoI CTYRAG 2 cut(s) 317, 669
Sse9I AATT 4 cut(s) 50, 274, 464, 475
SsiI CCGC 5 cut(s) 26, 33, 201, 418, 637
SspI AATATT 1 cut(s) 550
SspMI CTAG 3 cut(s) 63, 233, 510
StyD4I CCNGG 3 cut(s) 156, 357, 650
StyI CCWWGG 2 cut(s) 166, 624
TaaI ACNGT 2 cut(s) 335, 350
TaqI TCGA 2 cut(s) 329, 504
TasI AATT 4 cut(s) 50, 274, 464, 475
TatI WGTACW 1 cut(s) 427
Tru1I TTAA 3 cut(s) 459, 471, 543
Tru9I TTAA 3 cut(s) 459, 471, 543
TscAI CASTG 4 cut(s) 91, 353, 416, 538
TseFI GTSAC 2 cut(s) 497, 569
TseI GCWGC 2 cut(s) 66, 161
Tsp45I GTSAC 2 cut(s) 497, 569
TspDTI ATGAA 1 cut(s) 468
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 4 cut(s) 91, 353, 416, 538
Van91I CCANNNNNTGG 1 cut(s) 172
VneI GTGCAC 1 cut(s) 206
VpaK11BI GGWCC 2 cut(s) 154, 355
XapI RAATTY 1 cut(s) 475
XbaI TCTAGA 1 cut(s) 232
XspI CTAG 3 cut(s) 63, 233, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.