Rh3BG076400

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002605
Physical Location & Seq
Forward (+)
5994211 .. 5996679
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG076400.1

Sequence Viewer

Length: 624 bp
ATGAGTTCACTCGCTGCGAGATTCTCCAAGTTCATCAGAGTCTCTGTTCCTTCCTCGCAAAATTCCTCACTGGCAGTAGGCGGGCAAAGAAGCTCGAAGTTATGGTACTCGAGCGGCTCAATCGACAGGGACACTAATGACACAAAACAAGTTGGTGCCGAAGAAGATATTGACGATTTTCTTGGTGGCTTGAAAGATACAAATATGCAAGCCCAAGGTGTGGATCCCAAGAGGGGTTGGGGATTTCGCGGTGTGCACAAGGCAATTATTTGTGGAACAGTTGGACAATCCCCAGTGCAGAAGATCTTGAGGAATGGCCGGACTGTAACCATATTCACTGTTGGGACGGGGGGAATGTTTGACCAGAGATTACTGGGAAAGGACTTGCCAAGACCTGCTCAGTGGCACCGAATTGCTGTGCATAATGACGCACTTGGGGCTTATGCAGTTAAACAATTTGTTAAAAATTCATCAGTTTATGTTGAGGGTGACATCGAAACTAGAGTTTACAATGATAGCATCAATGGTGAAGTTAAGAATATTCCAGAGATCTGCATTCGTCATGATGGTATAGAAACCACAACTACAGTTTTTTTTTCCCCTCATTTAGTGTTTCTCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SSB

Protein Analysis

207

Amino Acids

22.8

Weight (kDa)

8.91

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 85 - 187 4.2e-18 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 403
AccB1I GGYRCC 2 cut(s) 155, 405
AccB7I CCANNNNNTGG 1 cut(s) 220
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 249
AciI CCGC 3 cut(s) 81, 114, 249
AclWI GGATC 2 cut(s) 218, 231
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 2 cut(s) 61, 466
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 220, 233
AgsI TTSAA 1 cut(s) 193
AluBI AGCT 1 cut(s) 93
AluI AGCT 1 cut(s) 93
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 46
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 2 cut(s) 218, 231
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 1 cut(s) 316
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 2 cut(s) 61, 466
AsuHPI GGTGA 2 cut(s) 500, 539
AvaI CYCGRG 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 258
BamHI GGATCC 1 cut(s) 223
BanI GGYRCC 2 cut(s) 155, 405
BarI GAAGNNNNNNTAC 2 cut(s) 89, 121
Bbv12I GWGCWC 1 cut(s) 258
BccI CCATC 1 cut(s) 560
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 403
BglII AGATCT 2 cut(s) 303, 549
BisI GCNGC 2 cut(s) 15, 115
BlsI GCNGC 2 cut(s) 16, 116
BmeT110I CYCGRG 1 cut(s) 109
BmiI GGNNCC 3 cut(s) 157, 225, 407
BmrI ACTGGG 2 cut(s) 287, 383
BmsI GCATC 1 cut(s) 528
BmuI ACTGGG 2 cut(s) 287, 383
BpuEI CTTGAG 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 214
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 233
Bse1I ACTGG 3 cut(s) 75, 293, 378
BseDI CCNNGG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 220, 233
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 3 cut(s) 75, 293, 378
BseSI GKGCMC 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 2 cut(s) 155, 405
BsiHKAI GWGCWC 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 319
BslFI GGGAC 2 cut(s) 143, 358
BslI CCNNNNNNNGG 2 cut(s) 220, 233
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 2 cut(s) 143, 358
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 1 cut(s) 318
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 3 cut(s) 223, 303, 549
BspACI CCGC 3 cut(s) 81, 114, 249
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 3 cut(s) 157, 225, 407
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 2 cut(s) 218, 231
BspT107I GGYRCC 2 cut(s) 155, 405
BsrBI CCGCTC 1 cut(s) 114
BsrI ACTGG 3 cut(s) 75, 293, 378
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 3 cut(s) 223, 303, 549
BssT1I CCWWGG 1 cut(s) 214
Bst4CI ACNGT 4 cut(s) 280, 325, 340, 589
BstC8I GCNNGC 2 cut(s) 83, 210
BstDEI CTNAG 1 cut(s) 399
BstFNI CGCG 1 cut(s) 249
BstKTI GATC 3 cut(s) 226, 306, 552
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 3 cut(s) 223, 303, 549
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstSFI CTRYAG 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 1 cut(s) 249
BstX2I RGATCY 3 cut(s) 223, 303, 549
BstYI RGATCY 3 cut(s) 223, 303, 549
BsuRI GGCC 1 cut(s) 318
BtsIMutI CAGTG 4 cut(s) 68, 300, 336, 407
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 2 cut(s) 83, 210
CciI TCATGA 1 cut(s) 562
CseI GACGC 1 cut(s) 437
Csp6I GTAC 1 cut(s) 106
CviAII CATG 1 cut(s) 563
CviJI RGCY 6 cut(s) 93, 117, 189, 212, 318, 440
CviKI_1 RGCY 6 cut(s) 93, 117, 189, 212, 318, 440
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 399
DpnI GATC 3 cut(s) 225, 305, 551
DpnII GATC 3 cut(s) 223, 303, 549
EaeI YGGCCR 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 214
FaeI CATG 1 cut(s) 566
FaiI YATR 8 cut(s) 103, 206, 332, 423, 444, 480, 564, 572
FaqI GGGAC 2 cut(s) 143, 358
FatI CATG 1 cut(s) 562
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 2 cut(s) 15, 115
Fsp4HI GCNGC 2 cut(s) 15, 115
FspBI CTAG 1 cut(s) 501
GluI GCNGC 2 cut(s) 15, 115
HaeIII GGCC 1 cut(s) 318
HapII CCGG 1 cut(s) 319
HgaI GACGC 1 cut(s) 437
Hin1II CATG 1 cut(s) 566
HinfI GANTC 2 cut(s) 21, 39
HpaII CCGG 1 cut(s) 319
HphI GGTGA 2 cut(s) 500, 539
Hpy166II GTNNAC 3 cut(s) 8, 256, 508
Hpy188I TCNGA 1 cut(s) 38
Hpy188III TCNNGA 3 cut(s) 307, 545, 563
Hpy8I GTNNAC 3 cut(s) 8, 256, 508
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 4 cut(s) 280, 325, 340, 589
HpyCH4V TGCA 6 cut(s) 208, 256, 298, 421, 446, 555
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 1 cut(s) 399
Hsp92II CATG 1 cut(s) 566
Kzo9I GATC 3 cut(s) 223, 303, 549
LpnPI CCDG 9 cut(s) 56, 112, 306, 332, 359, 377, 408, 558, 604
LweI GCATC 1 cut(s) 528
MaeI CTAG 1 cut(s) 501
MaeIII GTNAC 2 cut(s) 325, 488
MalI GATC 3 cut(s) 225, 305, 551
MbiI CCGCTC 1 cut(s) 114
MboI GATC 3 cut(s) 223, 303, 549
MboII GAAGA 3 cut(s) 173, 176, 313
MflI RGATCY 3 cut(s) 223, 303, 549
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 5 cut(s) 61, 264, 411, 455, 466
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 6 cut(s) 64, 76, 225, 303, 478, 612
MseI TTAA 3 cut(s) 450, 462, 534
MspI CCGG 1 cut(s) 319
Mva1269I GAATGC 1 cut(s) 555
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 437
NdeII GATC 3 cut(s) 223, 303, 549
NlaIII CATG 1 cut(s) 566
NlaIV GGNNCC 3 cut(s) 157, 225, 407
NmuCI GTSAC 1 cut(s) 488
PaeR7I CTCGAG 1 cut(s) 109
PagI TCATGA 1 cut(s) 562
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 1 cut(s) 21
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 2 cut(s) 16, 116
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PspN4I GGNNCC 3 cut(s) 157, 225, 407
PspXI VCTCGAGB 1 cut(s) 109
PsuI RGATCY 3 cut(s) 223, 303, 549
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SaqAI TTAA 3 cut(s) 450, 462, 534
SatI GCNGC 2 cut(s) 15, 115
Sau3AI GATC 3 cut(s) 223, 303, 549
SchI GAGTC 1 cut(s) 48
SduI GDGCHC 1 cut(s) 258
SetI ASST 3 cut(s) 95, 220, 397
SfaNI GCATC 1 cut(s) 528
SfcI CTRYAG 1 cut(s) 585
Sfr274I CTCGAG 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 109
SmlI CTYRAG 2 cut(s) 109, 307
SmoI CTYRAG 2 cut(s) 109, 307
Sse9I AATT 5 cut(s) 61, 264, 411, 455, 466
SsiI CCGC 3 cut(s) 81, 114, 249
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 501
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 4 cut(s) 280, 325, 340, 589
TaqI TCGA 4 cut(s) 95, 110, 123, 495
TasI AATT 5 cut(s) 61, 264, 411, 455, 466
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 3 cut(s) 450, 462, 534
Tru9I TTAA 3 cut(s) 450, 462, 534
TscAI CASTG 4 cut(s) 75, 300, 343, 407
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 22, 459
TspRI CASTG 4 cut(s) 75, 300, 343, 407
Van91I CCANNNNNTGG 1 cut(s) 220
VneI GTGCAC 1 cut(s) 254
XapI RAATTY 2 cut(s) 61, 466
XhoI CTCGAG 1 cut(s) 109
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.