MD04G1196700.v1.1

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002605
Physical Location & Seq
Reverse (-)
28495933 .. 28498512
2580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1196700.v1.1.491

Sequence Viewer

Length: 651 bp
ATGAGTTCAGTCGCTGCCAGATTCGTCAAGTTTATGAGGGTCTCCGTTCCTTCCTCGCCTACCTCCTCATTTGTTGGTGTGCAAAGAAGCTCAAAGTTGTGGTTCTCGAGCACCCCATCTGATTCTGACATTGGTAACGATGACACAAAGAACAATGTGGCAGAAGATGATTTTGATGACTTTATTGGTGCGAAGAAAGATGTGAATTTGCAACCCCAAGGTGTTGATCCCAGGAGGGGATGGGGATATCGTGGTGTGCACAAGGCAATTATCTGTGGAAAAGTTGGGCAAGCCCCTGTGCAAAAGATCTTGAGGAATGGTCGAACTGTAACCATCTTCACGGTTGGGACAGGAGGCATGTTCGACCAAAGGGTAATGGGAGCAAAGGACTTGCCAAGACCTGCTCAATGGCATCGAATTGCTGTGCATAATGAAGCACTTGGGGCTTATGCGGTTAAACAAGTGGTTAAGAATGCATCAATTTATGTCGAGGGTGACATTGAAACTAGAGTTTACAATGACAGCATCAATGGTGAAGTGAAACATATTCCAGAGATATGTATTCGTCACGATGGGAAGCTTCGTCTTATAAAGACTGGAGAAAGCATCAGCAGTATATCCTTGGATGATCTGCGAGAAGATTTGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SSB

Protein Analysis

217

Amino Acids

23.8

Weight (kDa)

8.85

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 86 - 189 7.3e-19 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
Acc36I ACCTGC 1 cut(s) 409
AciI CCGC 1 cut(s) 452
AclWI GGATC 1 cut(s) 221
AcsI RAATTY 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 1 cut(s) 503
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 2 cut(s) 90, 580
AluI AGCT 2 cut(s) 90, 580
Alw21I GWGCWC 2 cut(s) 113, 261
Alw26I GTCTC 1 cut(s) 46
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 1 cut(s) 221
AlwNI CAGNNNCTG 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 106
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 1 cut(s) 205
AsuHPI GGTGA 2 cut(s) 506, 545
AvaI CYCGRG 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 261
Bbv12I GWGCWC 2 cut(s) 113, 261
BccI CCATC 4 cut(s) 124, 234, 341, 566
BciT130I CCWGG 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 409
BglII AGATCT 1 cut(s) 306
BisI GCNGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 106
BmrFI CCNGG 1 cut(s) 232
BmsI GCATC 4 cut(s) 421, 485, 534, 615
BpmI CTGGAG 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 331
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 3 cut(s) 217, 230, 621
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 601
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 3 cut(s) 217, 230, 621
BseGI GGATG 2 cut(s) 245, 631
BseLI CCNNNNNNNGG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 1 cut(s) 55
BseSI GKGCMC 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 113, 261
BsiHKCI CYCGRG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 478
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 113, 261
Bsp143I GATC 3 cut(s) 226, 306, 628
BspACI CCGC 1 cut(s) 452
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 46
BsrI ACTGG 1 cut(s) 601
BssECI CCNNGG 3 cut(s) 217, 230, 621
BssMI GATC 3 cut(s) 226, 306, 628
BssT1I CCWWGG 2 cut(s) 217, 621
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 328, 343
BstC8I GCNNGC 1 cut(s) 291
BstF5I GGATG 2 cut(s) 245, 631
BstKTI GATC 3 cut(s) 229, 309, 631
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 3 cut(s) 226, 306, 628
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 230
BstSLI GKGCMC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BtsCI GGATG 2 cut(s) 245, 631
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 291
CaiI CAGNNNCTG 1 cut(s) 14
CviAII CATG 1 cut(s) 358
CviJI RGCY 4 cut(s) 90, 293, 446, 580
CviKI_1 RGCY 4 cut(s) 90, 293, 446, 580
DpnI GATC 3 cut(s) 228, 308, 630
DpnII GATC 3 cut(s) 226, 306, 628
Eco130I CCWWGG 2 cut(s) 217, 621
Eco31I GGTCTC 1 cut(s) 46
Eco32I GATATC 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 230
EcoRV GATATC 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 217, 621
EcoT22I ATGCAT 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 217, 621
FaeI CATG 1 cut(s) 361
FaiI YATR 9 cut(s) 35, 359, 429, 450, 486, 546, 559, 590, 617
FalI AAGNNNNNCTT 1 cut(s) 630
FaqI GGGAC 1 cut(s) 361
FatI CATG 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 15
FokI GGATG 2 cut(s) 252, 638
Fsp4HI GCNGC 1 cut(s) 15
FspBI CTAG 1 cut(s) 507
GluI GCNGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 618
Hin1II CATG 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 2 cut(s) 21, 122
HphI GGTGA 2 cut(s) 506, 545
Hpy166II GTNNAC 2 cut(s) 259, 514
Hpy188I TCNGA 2 cut(s) 121, 127
Hpy188III TCNNGA 4 cut(s) 106, 310, 551, 569
Hpy8I GTNNAC 2 cut(s) 259, 514
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 2 cut(s) 328, 343
HpyCH4V TGCA 6 cut(s) 82, 211, 259, 301, 427, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
Hsp92II CATG 1 cut(s) 361
Kzo9I GATC 3 cut(s) 226, 306, 628
LmnI GCTCC 1 cut(s) 380
LpnPI CCDG 8 cut(s) 31, 217, 244, 309, 336, 414, 564, 582
LweI GCATC 4 cut(s) 421, 485, 534, 615
MaeI CTAG 1 cut(s) 507
MaeIII GTNAC 4 cut(s) 134, 328, 494, 566
MalI GATC 3 cut(s) 228, 308, 630
MboI GATC 3 cut(s) 226, 306, 628
MboII GAAGA 4 cut(s) 176, 205, 328, 650
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 2 cut(s) 113, 261
MluCI AATT 4 cut(s) 205, 267, 417, 480
MnlI CCTC 8 cut(s) 30, 64, 73, 76, 228, 306, 347, 484
Mph1103I ATGCAT 1 cut(s) 478
MseI TTAA 2 cut(s) 456, 468
MspR9I CCNGG 1 cut(s) 232
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 1 cut(s) 232
MwoI GCNNNNNNNGC 1 cut(s) 443
NdeII GATC 3 cut(s) 226, 306, 628
NlaIII CATG 1 cut(s) 361
NmuCI GTSAC 2 cut(s) 494, 566
NsiI ATGCAT 1 cut(s) 478
NspI RCATGY 1 cut(s) 361
PaeR7I CTCGAG 1 cut(s) 106
PctI GAATGC 1 cut(s) 478
PfeI GAWTC 2 cut(s) 21, 122
PkrI GCNGC 1 cut(s) 16
PsiI TTATAA 1 cut(s) 590
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 306
SaqAI TTAA 2 cut(s) 456, 468
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 3 cut(s) 226, 306, 628
ScrFI CCNGG 1 cut(s) 232
SduI GDGCHC 2 cut(s) 113, 261
SetI ASST 5 cut(s) 65, 92, 223, 403, 582
SfaNI GCATC 4 cut(s) 421, 485, 534, 615
Sfr274I CTCGAG 1 cut(s) 106
SlaI CTCGAG 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 106, 310
SmoI CTYRAG 2 cut(s) 106, 310
Sse9I AATT 4 cut(s) 205, 267, 417, 480
SsiI CCGC 1 cut(s) 452
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 1 cut(s) 230
StyI CCWWGG 2 cut(s) 217, 621
TaaI ACNGT 2 cut(s) 328, 343
TaqI TCGA 5 cut(s) 107, 322, 363, 415, 489
TasI AATT 4 cut(s) 205, 267, 417, 480
TfiI GAWTC 2 cut(s) 21, 122
Tru1I TTAA 2 cut(s) 456, 468
Tru9I TTAA 2 cut(s) 456, 468
TseFI GTSAC 2 cut(s) 494, 566
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 2 cut(s) 494, 566
TspDTI ATGAA 1 cut(s) 447
TspGWI ACGGA 1 cut(s) 34
VneI GTGCAC 1 cut(s) 257
XapI RAATTY 1 cut(s) 205
XceI RCATGY 1 cut(s) 361
XhoI CTCGAG 1 cut(s) 106
XspI CTAG 1 cut(s) 507
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.