MD12G1209600.v1.1

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002605
Physical Location & Seq
Reverse (-)
28857441 .. 28860007
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1209600.v1.1.491

Sequence Viewer

Length: 651 bp
ATGAGTTCAGTCGCTGCGCGATTCGCCAAGTTTATGCGAGTCTCCGTTCCTTCCTCGCCCACCTCCTCACTCGTGGGTGTGCAAAGAAGCTCAAAGTTGTGGTGCTCAAGCATCCCATCTGACTCTGACATTGGCAATGATGACACAAAGAACAATGGGGCGGAAGATGGTTTCGACGACTTTATTGGTGCGAAGAAAGATGTGAATTTGCAACCCCAAGGAGTTGATCCCAGGAGGGGCTGGGGATTTCGTGGTGTGCACAAGGCAATTATCTGTGGAAAAGTTGGGCAAGCCCCTGTGCAAAAGATCTTGAGGAATGGTCGAACTGTAACCATCTTCACGGTTGGGACGGGAGGCATGTTTGACCAAAGGGTAATGGGAGCAAAGGACTTGCCAAGACCTGCTCAATGGCATCGAATTGCTGTGCATAATGAAGCACTTGGGGCTTATGCGGTTAAACAATTGGTTAAGAATGCATCAATCTATGTCGAGGGTGACATTGAAACTAGAGTTTACAATGACAGCATCAATGGCGAAGTGAAACATATTCCAGAGATATGCATTCGTCACGATGGGAAGCTTCGTCTTATAAAGACTGGAGAAAGCGTCAGCAGTATATCCTTGGATGATCTGCGAGAAGATTTGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SSB

Protein Analysis

217

Amino Acids

23.59

Weight (kDa)

8.99

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 86 - 189 5.1e-19 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
Acc36I ACCTGC 1 cut(s) 409
AccII CGCG 1 cut(s) 19
AciI CCGC 2 cut(s) 161, 452
AclWI GGATC 1 cut(s) 221
AcsI RAATTY 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 1 cut(s) 503
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 2 cut(s) 90, 580
AluI AGCT 2 cut(s) 90, 580
Alw21I GWGCWC 2 cut(s) 107, 261
Alw26I GTCTC 1 cut(s) 46
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 1 cut(s) 221
AlwNI CAGNNNCTG 1 cut(s) 14
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 1 cut(s) 205
AspLEI GCGC 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 261
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 2 cut(s) 107, 261
BccI CCATC 4 cut(s) 124, 161, 341, 566
BciT130I CCWGG 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 409
BglII AGATCT 1 cut(s) 306
BisI GCNGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 232
BmsI GCATC 4 cut(s) 120, 421, 485, 534
BpmI CTGGAG 1 cut(s) 618
BpuEI CTTGAG 2 cut(s) 91, 331
BsaJI CCNNGG 3 cut(s) 217, 230, 621
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 3 cut(s) 217, 230, 621
BseGI GGATG 2 cut(s) 111, 631
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMI GCAATG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 1 cut(s) 55
BseSI GKGCMC 1 cut(s) 261
BseYI CCCAGC 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 107, 261
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 361
BsmI GAATGC 2 cut(s) 478, 561
Bsp1286I GDGCHC 2 cut(s) 107, 261
Bsp143I GATC 3 cut(s) 226, 306, 628
BspACI CCGC 2 cut(s) 161, 452
BspFNI CGCG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 142
BsrI ACTGG 1 cut(s) 601
BssECI CCNNGG 3 cut(s) 217, 230, 621
BssMI GATC 3 cut(s) 226, 306, 628
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 2 cut(s) 217, 621
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 328, 343
BstC8I GCNNGC 1 cut(s) 291
BstF5I GGATG 2 cut(s) 111, 631
BstFNI CGCG 1 cut(s) 19
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 3 cut(s) 229, 309, 631
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 3 cut(s) 226, 306, 628
BstMWI GCNNNNNNNGC 3 cut(s) 23, 443, 531
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 230
BstSLI GKGCMC 1 cut(s) 261
BstUI CGCG 1 cut(s) 19
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BtsCI GGATG 2 cut(s) 111, 631
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 291
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 1 cut(s) 19
CseI GACGC 1 cut(s) 595
CviAII CATG 1 cut(s) 358
CviJI RGCY 5 cut(s) 90, 240, 293, 446, 580
CviKI_1 RGCY 5 cut(s) 90, 240, 293, 446, 580
DpnI GATC 3 cut(s) 228, 308, 630
DpnII GATC 3 cut(s) 226, 306, 628
EciI GGCGGA 1 cut(s) 176
Eco130I CCWWGG 2 cut(s) 217, 621
EcoRII CCWGG 1 cut(s) 230
EcoT14I CCWWGG 2 cut(s) 217, 621
EcoT22I ATGCAT 2 cut(s) 478, 563
ErhI CCWWGG 2 cut(s) 217, 621
FaeI CATG 1 cut(s) 361
FaiI YATR 9 cut(s) 35, 359, 429, 450, 486, 546, 559, 590, 617
FalI AAGNNNNNCTT 1 cut(s) 630
FaqI GGGAC 1 cut(s) 361
FatI CATG 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 15
FokI GGATG 2 cut(s) 98, 638
Fsp4HI GCNGC 1 cut(s) 15
FspBI CTAG 1 cut(s) 507
GlaI GCGC 1 cut(s) 18
GluI GCNGC 1 cut(s) 15
GsaI CCCAGC 1 cut(s) 244
GsuI CTGGAG 1 cut(s) 618
HgaI GACGC 1 cut(s) 595
HhaI GCGC 1 cut(s) 19
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 3 cut(s) 21, 39, 122
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 2 cut(s) 259, 514
Hpy188I TCNGA 2 cut(s) 121, 127
Hpy188III TCNNGA 3 cut(s) 310, 551, 569
Hpy8I GTNNAC 2 cut(s) 259, 514
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 2 cut(s) 328, 343
HpyCH4V TGCA 7 cut(s) 82, 211, 259, 301, 427, 476, 561
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 443, 531
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 3 cut(s) 226, 306, 628
LmnI GCTCC 1 cut(s) 380
LpnPI CCDG 7 cut(s) 217, 226, 244, 309, 414, 564, 582
LweI GCATC 4 cut(s) 120, 421, 485, 534
MaeI CTAG 1 cut(s) 507
MaeIII GTNAC 3 cut(s) 328, 494, 566
MalI GATC 3 cut(s) 228, 308, 630
MboI GATC 3 cut(s) 226, 306, 628
MboII GAAGA 4 cut(s) 176, 205, 328, 650
MfeI CAATTG 1 cut(s) 461
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 2 cut(s) 107, 261
MluCI AATT 4 cut(s) 205, 267, 417, 461
MlyI GAGTC 2 cut(s) 48, 116
MnlI CCTC 7 cut(s) 64, 73, 76, 228, 306, 347, 484
Mph1103I ATGCAT 2 cut(s) 478, 563
MseI TTAA 2 cut(s) 456, 468
MspR9I CCNGG 1 cut(s) 232
MunI CAATTG 1 cut(s) 461
Mva1269I GAATGC 2 cut(s) 478, 561
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 3 cut(s) 23, 443, 531
NdeII GATC 3 cut(s) 226, 306, 628
NlaIII CATG 1 cut(s) 361
NmuCI GTSAC 2 cut(s) 494, 566
NsiI ATGCAT 2 cut(s) 478, 563
NspI RCATGY 1 cut(s) 361
PctI GAATGC 2 cut(s) 478, 561
PfeI GAWTC 1 cut(s) 21
PkrI GCNGC 1 cut(s) 16
PleI GAGTC 2 cut(s) 47, 116
PpsI GAGTC 2 cut(s) 47, 116
PsiI TTATAA 1 cut(s) 590
Psp6I CCWGG 1 cut(s) 230
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 306
SaqAI TTAA 2 cut(s) 456, 468
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 3 cut(s) 226, 306, 628
SchI GAGTC 2 cut(s) 48, 116
ScrFI CCNGG 1 cut(s) 232
SduI GDGCHC 2 cut(s) 107, 261
SetI ASST 4 cut(s) 65, 92, 403, 582
SfaNI GCATC 4 cut(s) 120, 421, 485, 534
SmlI CTYRAG 2 cut(s) 106, 310
SmoI CTYRAG 2 cut(s) 106, 310
Sse9I AATT 4 cut(s) 205, 267, 417, 461
SsiI CCGC 2 cut(s) 161, 452
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 1 cut(s) 230
StyI CCWWGG 2 cut(s) 217, 621
TaaI ACNGT 2 cut(s) 328, 343
TaqI TCGA 4 cut(s) 174, 322, 415, 489
TasI AATT 4 cut(s) 205, 267, 417, 461
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 2 cut(s) 456, 468
Tru9I TTAA 2 cut(s) 456, 468
TseFI GTSAC 2 cut(s) 494, 566
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 2 cut(s) 494, 566
TspDTI ATGAA 1 cut(s) 447
TspGWI ACGGA 1 cut(s) 34
VneI GTGCAC 1 cut(s) 257
XapI RAATTY 1 cut(s) 205
XceI RCATGY 1 cut(s) 361
XspI CTAG 1 cut(s) 507
Zsp2I ATGCAT 2 cut(s) 478, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.