Rh3AG074700

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002605
Physical Location & Seq
Forward (+)
5994211 .. 5996679
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG074700.1

Sequence Viewer

Length: 510 bp
ATGCAAGCCCAAGGTGTGGATCCCAAGAGGGGTTGGGGATTTCGCGGTGTGCACAAGGCAATTATTTGTGGAACAGTTGGACAATCCCCAGTGCAGAAGATCTTGAGGAATGGCCGGACTGTAACCATATTCACTGTTGGGACGGGGGGAATGTTTGACCAGAGATTACTGGGAAAGGACTTGCCAAGACCTGCTCAGTGGCACCGAATTGCTGTGCATAATGACGCACTTGGGGCTTATGCAGTTAAACAATTTGTTAAAAATTCATCAGTTTATGTTGAGGGTGACATCGAAACTAGAGTTTACAATGATAGCATCAATGGTGAAGTTAAGAATATTCCAGAGATCTGCATTCGTCATGATGGGAAGATCCGTCTTATAAAGAGTGGAGAAAGCATCAGCAGTATGTCCTTGGATGATCTGCGTAAGTCCCTTTTACTTCTGCTCTTTCTGCTTTCTCCCACATGTGAGACTTCAATGTGTTTGAGTTCTACCATTTCACTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SSB

Protein Analysis

169

Amino Acids

18.65

Weight (kDa)

9.32

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 17 - 119 1.9e-18 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
Acc36I ACCTGC 1 cut(s) 199
AccB1I GGYRCC 1 cut(s) 201
AccB7I CCANNNNNTGG 1 cut(s) 16
AccII CGCG 1 cut(s) 45
AciI CCGC 1 cut(s) 45
AclWI GGATC 3 cut(s) 14, 27, 364
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 1 cut(s) 262
AfiI CCNNNNNNNGG 2 cut(s) 16, 29
AflIII ACRYGT 1 cut(s) 464
AgsI TTSAA 1 cut(s) 477
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 1 cut(s) 464
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 3 cut(s) 14, 27, 364
AoxI GGCC 1 cut(s) 112
ApaLI GTGCAC 1 cut(s) 50
ApoI RAATTY 1 cut(s) 262
AsuHPI GGTGA 2 cut(s) 296, 335
BaeGI GKGCMC 1 cut(s) 54
BamHI GGATCC 1 cut(s) 19
BanI GGYRCC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 54
BccI CCATC 1 cut(s) 356
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 297
BfuAI ACCTGC 1 cut(s) 199
BglII AGATCT 2 cut(s) 99, 345
BmiI GGNNCC 2 cut(s) 21, 203
BmrI ACTGGG 2 cut(s) 83, 179
BmsI GCATC 2 cut(s) 324, 405
BmuI ACTGGG 2 cut(s) 83, 179
BpuEI CTTGAG 1 cut(s) 124
BsaJI CCNNGG 2 cut(s) 10, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 29
Bse1I ACTGG 2 cut(s) 89, 174
BseDI CCNNGG 2 cut(s) 10, 411
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 2 cut(s) 16, 29
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 2 cut(s) 89, 174
BseSI GKGCMC 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 113
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 114
BshNI GGYRCC 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 1 cut(s) 115
BslFI GGGAC 2 cut(s) 154, 415
BslI CCNNNNNNNGG 2 cut(s) 16, 29
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 2 cut(s) 154, 415
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 5 cut(s) 19, 99, 345, 369, 418
BspACI CCGC 1 cut(s) 45
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 2 cut(s) 21, 203
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 3 cut(s) 14, 27, 364
BspT107I GGYRCC 1 cut(s) 201
BsrI ACTGG 2 cut(s) 89, 174
BssECI CCNNGG 2 cut(s) 10, 411
BssMI GATC 5 cut(s) 19, 99, 345, 369, 418
BssT1I CCWWGG 2 cut(s) 10, 411
Bst4CI ACNGT 3 cut(s) 76, 121, 136
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 421
BstFNI CGCG 1 cut(s) 45
BstKTI GATC 5 cut(s) 22, 102, 348, 372, 421
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 5 cut(s) 19, 99, 345, 369, 418
BstMWI GCNNNNNNNGC 2 cut(s) 233, 451
BstNSI RCATGY 1 cut(s) 468
BstSLI GKGCMC 1 cut(s) 54
BstUI CGCG 1 cut(s) 45
BstX2I RGATCY 4 cut(s) 19, 99, 345, 369
BstYI RGATCY 4 cut(s) 19, 99, 345, 369
BsuRI GGCC 1 cut(s) 114
BtsCI GGATG 1 cut(s) 421
BtsIMutI CAGTG 3 cut(s) 96, 132, 203
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 6
CciI TCATGA 1 cut(s) 358
CseI GACGC 1 cut(s) 233
CviAII CATG 2 cut(s) 359, 465
CviJI RGCY 3 cut(s) 8, 114, 236
CviKI_1 RGCY 3 cut(s) 8, 114, 236
DdeI CTNAG 1 cut(s) 195
DpnI GATC 5 cut(s) 21, 101, 347, 371, 420
DpnII GATC 5 cut(s) 19, 99, 345, 369, 418
EaeI YGGCCR 1 cut(s) 112
Eco130I CCWWGG 2 cut(s) 10, 411
EcoT14I CCWWGG 2 cut(s) 10, 411
ErhI CCWWGG 2 cut(s) 10, 411
FaeI CATG 2 cut(s) 362, 468
FaiI YATR 8 cut(s) 128, 219, 240, 276, 360, 380, 407, 466
FaqI GGGAC 2 cut(s) 154, 415
FatI CATG 2 cut(s) 358, 464
FokI GGATG 1 cut(s) 428
FspBI CTAG 1 cut(s) 297
HaeIII GGCC 1 cut(s) 114
HapII CCGG 1 cut(s) 115
HgaI GACGC 1 cut(s) 233
Hin1II CATG 2 cut(s) 362, 468
HpaII CCGG 1 cut(s) 115
HphI GGTGA 2 cut(s) 296, 335
Hpy166II GTNNAC 2 cut(s) 52, 304
Hpy188I TCNGA 1 cut(s) 509
Hpy188III TCNNGA 3 cut(s) 103, 341, 359
Hpy8I GTNNAC 2 cut(s) 52, 304
HpyCH4III ACNGT 3 cut(s) 76, 121, 136
HpyCH4V TGCA 6 cut(s) 4, 52, 94, 217, 242, 351
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 451
HpyF3I CTNAG 1 cut(s) 195
Hsp92II CATG 2 cut(s) 362, 468
Kzo9I GATC 5 cut(s) 19, 99, 345, 369, 418
LpnPI CCDG 6 cut(s) 102, 128, 155, 173, 204, 354
LweI GCATC 2 cut(s) 324, 405
MaeI CTAG 1 cut(s) 297
MaeIII GTNAC 2 cut(s) 121, 284
MalI GATC 5 cut(s) 21, 101, 347, 371, 420
MboI GATC 5 cut(s) 19, 99, 345, 369, 418
MboII GAAGA 2 cut(s) 109, 379
MflI RGATCY 4 cut(s) 19, 99, 345, 369
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 4 cut(s) 60, 207, 251, 262
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 3 cut(s) 21, 99, 274
MseI TTAA 3 cut(s) 246, 258, 330
MspI CCGG 1 cut(s) 115
Mva1269I GAATGC 1 cut(s) 351
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 233, 451
NdeII GATC 5 cut(s) 19, 99, 345, 369, 418
NlaIII CATG 2 cut(s) 362, 468
NlaIV GGNNCC 2 cut(s) 21, 203
NmuCI GTSAC 1 cut(s) 284
NspI RCATGY 1 cut(s) 468
PagI TCATGA 1 cut(s) 358
PciI ACATGT 1 cut(s) 464
PctI GAATGC 1 cut(s) 351
PflMI CCANNNNNTGG 1 cut(s) 16
PscI ACATGT 1 cut(s) 464
PsiI TTATAA 1 cut(s) 380
PspN4I GGNNCC 2 cut(s) 21, 203
PsuI RGATCY 4 cut(s) 19, 99, 345, 369
SaqAI TTAA 3 cut(s) 246, 258, 330
Sau3AI GATC 5 cut(s) 19, 99, 345, 369, 418
SduI GDGCHC 1 cut(s) 54
SetI ASST 2 cut(s) 16, 193
SfaNI GCATC 2 cut(s) 324, 405
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
Sse9I AATT 4 cut(s) 60, 207, 251, 262
SsiI CCGC 1 cut(s) 45
SspI AATATT 1 cut(s) 337
SspMI CTAG 1 cut(s) 297
StyI CCWWGG 2 cut(s) 10, 411
TaaI ACNGT 3 cut(s) 76, 121, 136
TaqI TCGA 1 cut(s) 291
TasI AATT 4 cut(s) 60, 207, 251, 262
Tru1I TTAA 3 cut(s) 246, 258, 330
Tru9I TTAA 3 cut(s) 246, 258, 330
TscAI CASTG 3 cut(s) 96, 139, 203
TseFI GTSAC 1 cut(s) 284
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 1 cut(s) 255
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 3 cut(s) 96, 139, 203
Van91I CCANNNNNTGG 1 cut(s) 16
VneI GTGCAC 1 cut(s) 50
XapI RAATTY 1 cut(s) 262
XceI RCATGY 1 cut(s) 468
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.