Rroxscaffold_6G00422620

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002605
Physical Location & Seq
Reverse (-)
43448980 .. 43451564
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422620.1

Sequence Viewer

Length: 645 bp
ATGAGTTCACTCGCTGCGAGATTTTCCAAGTTCATCAGAGTCTCTGTTCCTTCCTCGCAAACTTCCTCACTGGCAGTAGGTGGGCAAAGAAGCTCGAAGTTATGGTACTCGGGCGGCTCAATCGACAGTGACACTAATGACACAAAACAAGTTGGTGCCGAAGAAGATATTGATGATTTTCTTGGTGGCTTGAAAGATACAAATATGCAAGCCCAAGGTGTGGATCCCAAGAGGGGTTGGGGATTTCGCGGTGTGCACAAGGCAATTATTTGTGGAAGAGTTGGACAATCCCCAGTGCAGAAGATCTTGAGGAATGGCCGAACTGTAACCATCTTCACTGTTGGGACGGGGGGCATGTTTGACCAGAGATTACTGGGAAAGGACTTGCCAAGACCTGCTCAGTGGCACCGAATTGCTGTGCATAATGACGCACTTGGGGCTTATGCAGTTAAACAATTTGTTAAAAATTCATCAGTTTATGTTGAGGGTGACATCGAAACTAGAGTTTACAATGATAGCATCAATGGTGAAGTTAAGAATATTCCAGAGATCTGCATTCGTCATGATGGGAAGATCCGTCTTATAAAGAGTGGAGAAAGCATCAGCAGTATGTCCTTGGATGATCTGCGAGAAGACTTACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SSB

Protein Analysis

214

Amino Acids

23.41

Weight (kDa)

8.87

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 85 - 187 1.1e-18 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
Acc36I ACCTGC 1 cut(s) 403
AccB1I GGYRCC 2 cut(s) 155, 405
AccB7I CCANNNNNTGG 1 cut(s) 220
AccII CGCG 1 cut(s) 249
AciI CCGC 2 cut(s) 114, 249
AclWI GGATC 3 cut(s) 218, 231, 568
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 1 cut(s) 466
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 220, 233
AgsI TTSAA 1 cut(s) 193
AluBI AGCT 1 cut(s) 93
AluI AGCT 1 cut(s) 93
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 46
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 3 cut(s) 218, 231, 568
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 1 cut(s) 316
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 1 cut(s) 466
AsuHPI GGTGA 2 cut(s) 500, 539
AvaI CYCGRG 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 258
BamHI GGATCC 1 cut(s) 223
BanI GGYRCC 2 cut(s) 155, 405
BarI GAAGNNNNNNTAC 2 cut(s) 89, 121
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 258
BccI CCATC 2 cut(s) 338, 560
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 501
BfuAI ACCTGC 1 cut(s) 403
BglII AGATCT 2 cut(s) 303, 549
BisI GCNGC 2 cut(s) 15, 115
BlsI GCNGC 2 cut(s) 16, 116
BmeT110I CYCGRG 1 cut(s) 109
BmiI GGNNCC 3 cut(s) 157, 225, 407
BmrI ACTGGG 2 cut(s) 287, 383
BmsI GCATC 2 cut(s) 528, 609
BmuI ACTGGG 2 cut(s) 287, 383
BpiI GAAGAC 1 cut(s) 639
BpuEI CTTGAG 1 cut(s) 328
BsaJI CCNNGG 2 cut(s) 214, 615
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 233
Bse1I ACTGG 3 cut(s) 75, 293, 378
BseDI CCNNGG 2 cut(s) 214, 615
BseGI GGATG 1 cut(s) 625
BseLI CCNNNNNNNGG 2 cut(s) 220, 233
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 3 cut(s) 75, 293, 378
BseSI GKGCMC 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 2 cut(s) 155, 405
BsiHKAI GWGCWC 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 109
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 220, 233
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 358
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 1 cut(s) 318
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 5 cut(s) 223, 303, 549, 573, 622
BspACI CCGC 2 cut(s) 114, 249
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 3 cut(s) 157, 225, 407
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 3 cut(s) 218, 231, 568
BspT107I GGYRCC 2 cut(s) 155, 405
BsrI ACTGG 3 cut(s) 75, 293, 378
BssECI CCNNGG 2 cut(s) 214, 615
BssMI GATC 5 cut(s) 223, 303, 549, 573, 622
BssT1I CCWWGG 2 cut(s) 214, 615
Bst4CI ACNGT 3 cut(s) 128, 325, 340
Bst6I CTCTTC 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 2 cut(s) 399, 642
BstF5I GGATG 1 cut(s) 625
BstFNI CGCG 1 cut(s) 249
BstKTI GATC 5 cut(s) 226, 306, 552, 576, 625
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 5 cut(s) 223, 303, 549, 573, 622
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNSI RCATGY 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 1 cut(s) 249
BstV2I GAAGAC 1 cut(s) 639
BstX2I RGATCY 4 cut(s) 223, 303, 549, 573
BstYI RGATCY 4 cut(s) 223, 303, 549, 573
BsuRI GGCC 1 cut(s) 318
BtsCI GGATG 1 cut(s) 625
BtsIMutI CAGTG 5 cut(s) 68, 133, 300, 336, 407
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 210
CciI TCATGA 1 cut(s) 562
CseI GACGC 1 cut(s) 437
Csp6I GTAC 1 cut(s) 106
CviAII CATG 2 cut(s) 355, 563
CviJI RGCY 6 cut(s) 93, 117, 189, 212, 318, 440
CviKI_1 RGCY 6 cut(s) 93, 117, 189, 212, 318, 440
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 2 cut(s) 399, 642
DpnI GATC 5 cut(s) 225, 305, 551, 575, 624
DpnII GATC 5 cut(s) 223, 303, 549, 573, 622
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 214, 615
Eco88I CYCGRG 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 214, 615
ErhI CCWWGG 2 cut(s) 214, 615
FaeI CATG 2 cut(s) 358, 566
FaiI YATR 9 cut(s) 103, 206, 356, 423, 444, 480, 564, 584, 611
FaqI GGGAC 1 cut(s) 358
FatI CATG 2 cut(s) 354, 562
Fnu4HI GCNGC 2 cut(s) 15, 115
FokI GGATG 1 cut(s) 632
Fsp4HI GCNGC 2 cut(s) 15, 115
FspBI CTAG 1 cut(s) 501
GluI GCNGC 2 cut(s) 15, 115
HaeIII GGCC 1 cut(s) 318
HgaI GACGC 1 cut(s) 437
Hin1II CATG 2 cut(s) 358, 566
HinfI GANTC 1 cut(s) 39
HphI GGTGA 2 cut(s) 500, 539
Hpy166II GTNNAC 3 cut(s) 8, 256, 508
Hpy188I TCNGA 1 cut(s) 38
Hpy188III TCNNGA 3 cut(s) 307, 545, 563
Hpy8I GTNNAC 3 cut(s) 8, 256, 508
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 3 cut(s) 128, 325, 340
HpyCH4V TGCA 6 cut(s) 208, 256, 298, 421, 446, 555
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 2 cut(s) 399, 642
Hsp92II CATG 2 cut(s) 358, 566
Kzo9I GATC 5 cut(s) 223, 303, 549, 573, 622
LpnPI CCDG 6 cut(s) 56, 306, 359, 377, 408, 558
LweI GCATC 2 cut(s) 528, 609
MaeI CTAG 1 cut(s) 501
MaeIII GTNAC 3 cut(s) 128, 325, 488
MalI GATC 5 cut(s) 225, 305, 551, 575, 624
MboI GATC 5 cut(s) 223, 303, 549, 573, 622
MboII GAAGA 7 cut(s) 173, 176, 288, 313, 325, 583, 644
MflI RGATCY 4 cut(s) 223, 303, 549, 573
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 4 cut(s) 264, 411, 455, 466
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 5 cut(s) 64, 76, 225, 303, 478
MseI TTAA 3 cut(s) 450, 462, 534
Mva1269I GAATGC 1 cut(s) 555
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 437
NdeII GATC 5 cut(s) 223, 303, 549, 573, 622
NlaIII CATG 2 cut(s) 358, 566
NlaIV GGNNCC 3 cut(s) 157, 225, 407
NmuCI GTSAC 2 cut(s) 128, 488
NspI RCATGY 1 cut(s) 358
PagI TCATGA 1 cut(s) 562
PctI GAATGC 1 cut(s) 555
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 2 cut(s) 16, 116
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PsiI TTATAA 1 cut(s) 584
PspN4I GGNNCC 3 cut(s) 157, 225, 407
PsuI RGATCY 4 cut(s) 223, 303, 549, 573
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SaqAI TTAA 3 cut(s) 450, 462, 534
SatI GCNGC 2 cut(s) 15, 115
Sau3AI GATC 5 cut(s) 223, 303, 549, 573, 622
SchI GAGTC 1 cut(s) 48
SduI GDGCHC 1 cut(s) 258
SetI ASST 5 cut(s) 82, 95, 220, 397, 643
SfaNI GCATC 2 cut(s) 528, 609
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 4 cut(s) 264, 411, 455, 466
SsiI CCGC 2 cut(s) 114, 249
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 501
StyI CCWWGG 2 cut(s) 214, 615
TaaI ACNGT 3 cut(s) 128, 325, 340
TaqI TCGA 3 cut(s) 95, 123, 495
TasI AATT 4 cut(s) 264, 411, 455, 466
TauI GCSGC 1 cut(s) 117
Tru1I TTAA 3 cut(s) 450, 462, 534
Tru9I TTAA 3 cut(s) 450, 462, 534
TscAI CASTG 5 cut(s) 75, 133, 300, 343, 407
TseFI GTSAC 2 cut(s) 128, 488
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 2 cut(s) 128, 488
TspDTI ATGAA 2 cut(s) 22, 459
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 5 cut(s) 75, 133, 300, 343, 407
Van91I CCANNNNNTGG 1 cut(s) 220
VneI GTGCAC 1 cut(s) 254
XapI RAATTY 1 cut(s) 466
XceI RCATGY 1 cut(s) 358
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.