Rw3G006390

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002605
Physical Location & Seq
Forward (+)
6049423 .. 6052082
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G006390.1

Sequence Viewer

Length: 672 bp
ATGAGTTCACTCGCTGCGAGATTTTCCAAGTTCATCAGAGTCTCTGTTCCTTCCTCGCAAACTTCCTCACTGGCAGTAGGCGGGCAAAGAAGCTCGAAGTTATGGTATTCGAGCGGCTCAATCGACAGTGACACTAATGACGCAAAACAACTTGGTGCCGAAGAAGATATTGACGATTTTCTTGGTGGCTTGAAAGATACAAATATGCAAGCCCAAGGTGTGGATCCCAAGAGGGGTTGGGGATTTCGCGGTGTGCACAAGGCAATTATTTGTGGAAGAGTTGGACAATCCCCAGTGCAGAAGATCTTGAGGAATGGCCGAACTGTAACCATCTTCACTGTTGGGACGGGGGGCATGTTTGACCAGAGATTACTGGGAAAGGACTTGCCAAGACCTGCTCAGTGGCACCGAATTGCTGTGCATAATGACGCACTTGGGGCTTATGCAGTTAAACAATTTGTTAAGAATTCATCAGTTTATGTTGAGGGTGACATCGAAACTAGAGTTTACAATGATAGCATCAATGGTGAAGTTAAGAATATTCCAGAGATCTGCATTCGTCACGATGGGAAGATCCGTCTTATAAAGAGTGGAGAAAGCATCAGCAGTATGTCCTTGGATGATCTGCTGAATACACTCTCTCTAAAGAATCGACTGGATGGTACCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SSB

Protein Analysis

223

Amino Acids

24.35

Weight (kDa)

9.25

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 85 - 187 1.2e-18 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
Acc36I ACCTGC 1 cut(s) 403
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 3 cut(s) 155, 405, 662
AccB7I CCANNNNNTGG 1 cut(s) 220
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 249
AciI CCGC 3 cut(s) 81, 114, 249
AclWI GGATC 3 cut(s) 218, 231, 568
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 1 cut(s) 466
AfaI GTAC 1 cut(s) 664
AfiI CCNNNNNNNGG 2 cut(s) 220, 233
AgsI TTSAA 1 cut(s) 193
AluBI AGCT 1 cut(s) 93
AluI AGCT 1 cut(s) 93
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 46
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 3 cut(s) 218, 231, 568
AoxI GGCC 1 cut(s) 316
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 1 cut(s) 466
Asp718I GGTACC 1 cut(s) 662
AsuHPI GGTGA 2 cut(s) 500, 539
BaeGI GKGCMC 1 cut(s) 258
BamHI GGATCC 1 cut(s) 223
BanI GGYRCC 3 cut(s) 155, 405, 662
Bbv12I GWGCWC 1 cut(s) 258
BccI CCATC 3 cut(s) 338, 560, 653
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 501
BfuAI ACCTGC 1 cut(s) 403
BglII AGATCT 2 cut(s) 303, 549
BisI GCNGC 2 cut(s) 15, 115
BlsI GCNGC 2 cut(s) 16, 116
BmiI GGNNCC 4 cut(s) 157, 225, 407, 664
BmrI ACTGGG 2 cut(s) 287, 383
BmsI GCATC 2 cut(s) 528, 609
BmuI ACTGGG 2 cut(s) 287, 383
BpuEI CTTGAG 1 cut(s) 328
BsaJI CCNNGG 2 cut(s) 214, 615
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 233
Bse1I ACTGG 4 cut(s) 75, 293, 378, 660
BseDI CCNNGG 2 cut(s) 214, 615
BseGI GGATG 2 cut(s) 625, 664
BseLI CCNNNNNNNGG 2 cut(s) 220, 233
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 4 cut(s) 75, 293, 378, 660
BseSI GKGCMC 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 3 cut(s) 155, 405, 662
BsiHKAI GWGCWC 1 cut(s) 258
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 220, 233
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 358
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 5 cut(s) 223, 303, 549, 573, 622
BspACI CCGC 3 cut(s) 81, 114, 249
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 249
BspLI GGNNCC 4 cut(s) 157, 225, 407, 664
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 3 cut(s) 218, 231, 568
BspT107I GGYRCC 3 cut(s) 155, 405, 662
BsrBI CCGCTC 1 cut(s) 114
BsrI ACTGG 4 cut(s) 75, 293, 378, 660
BssECI CCNNGG 2 cut(s) 214, 615
BssMI GATC 5 cut(s) 223, 303, 549, 573, 622
BssT1I CCWWGG 2 cut(s) 214, 615
Bst4CI ACNGT 3 cut(s) 128, 325, 340
Bst6I CTCTTC 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 83, 210
BstDEI CTNAG 1 cut(s) 399
BstF5I GGATG 2 cut(s) 625, 664
BstFNI CGCG 1 cut(s) 249
BstKTI GATC 5 cut(s) 226, 306, 552, 576, 625
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 5 cut(s) 223, 303, 549, 573, 622
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNSI RCATGY 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 1 cut(s) 249
BstX2I RGATCY 4 cut(s) 223, 303, 549, 573
BstYI RGATCY 4 cut(s) 223, 303, 549, 573
BsuRI GGCC 1 cut(s) 318
BtsCI GGATG 2 cut(s) 625, 664
BtsIMutI CAGTG 5 cut(s) 68, 133, 300, 336, 407
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 2 cut(s) 83, 210
CseI GACGC 2 cut(s) 149, 437
Csp6I GTAC 1 cut(s) 663
CviAII CATG 1 cut(s) 355
CviJI RGCY 6 cut(s) 93, 117, 189, 212, 318, 440
CviKI_1 RGCY 6 cut(s) 93, 117, 189, 212, 318, 440
CviQI GTAC 1 cut(s) 663
DdeI CTNAG 1 cut(s) 399
DpnI GATC 5 cut(s) 225, 305, 551, 575, 624
DpnII GATC 5 cut(s) 223, 303, 549, 573, 622
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 214, 615
EcoRI GAATTC 1 cut(s) 466
EcoT14I CCWWGG 2 cut(s) 214, 615
ErhI CCWWGG 2 cut(s) 214, 615
FaeI CATG 1 cut(s) 358
FaiI YATR 8 cut(s) 103, 206, 356, 423, 444, 480, 584, 611
FaqI GGGAC 1 cut(s) 358
FatI CATG 1 cut(s) 354
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 2 cut(s) 15, 115
FokI GGATG 1 cut(s) 632
Fsp4HI GCNGC 2 cut(s) 15, 115
FspBI CTAG 1 cut(s) 501
GluI GCNGC 2 cut(s) 15, 115
HaeIII GGCC 1 cut(s) 318
HgaI GACGC 2 cut(s) 149, 437
Hin1II CATG 1 cut(s) 358
HinfI GANTC 2 cut(s) 39, 649
HphI GGTGA 2 cut(s) 500, 539
Hpy166II GTNNAC 3 cut(s) 8, 256, 508
Hpy188I TCNGA 1 cut(s) 38
Hpy188III TCNNGA 3 cut(s) 307, 545, 563
Hpy8I GTNNAC 3 cut(s) 8, 256, 508
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 3 cut(s) 128, 325, 340
HpyCH4V TGCA 6 cut(s) 208, 256, 298, 421, 446, 555
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 1 cut(s) 399
Hsp92II CATG 1 cut(s) 358
KpnI GGTACC 1 cut(s) 666
Kzo9I GATC 5 cut(s) 223, 303, 549, 573, 622
LpnPI CCDG 7 cut(s) 56, 306, 359, 377, 408, 558, 641
LweI GCATC 2 cut(s) 528, 609
MaeI CTAG 1 cut(s) 501
MaeIII GTNAC 4 cut(s) 128, 325, 488, 560
MalI GATC 5 cut(s) 225, 305, 551, 575, 624
MbiI CCGCTC 1 cut(s) 114
MboI GATC 5 cut(s) 223, 303, 549, 573, 622
MboII GAAGA 6 cut(s) 173, 176, 288, 313, 325, 583
MflI RGATCY 4 cut(s) 223, 303, 549, 573
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 4 cut(s) 264, 411, 455, 466
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 5 cut(s) 64, 76, 225, 303, 478
MseI TTAA 3 cut(s) 450, 462, 534
Mva1269I GAATGC 1 cut(s) 555
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 437
NdeII GATC 5 cut(s) 223, 303, 549, 573, 622
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 4 cut(s) 157, 225, 407, 664
NmuCI GTSAC 3 cut(s) 128, 488, 560
NspI RCATGY 1 cut(s) 358
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 1 cut(s) 649
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 2 cut(s) 16, 116
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PsiI TTATAA 1 cut(s) 584
PspN4I GGNNCC 4 cut(s) 157, 225, 407, 664
PsuI RGATCY 4 cut(s) 223, 303, 549, 573
RsaI GTAC 1 cut(s) 664
RsaNI GTAC 1 cut(s) 663
SaqAI TTAA 3 cut(s) 450, 462, 534
SatI GCNGC 2 cut(s) 15, 115
Sau3AI GATC 5 cut(s) 223, 303, 549, 573, 622
SchI GAGTC 1 cut(s) 48
SduI GDGCHC 1 cut(s) 258
SetI ASST 3 cut(s) 95, 220, 397
SfaNI GCATC 2 cut(s) 528, 609
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 4 cut(s) 264, 411, 455, 466
SsiI CCGC 3 cut(s) 81, 114, 249
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 501
StyI CCWWGG 2 cut(s) 214, 615
TaaI ACNGT 3 cut(s) 128, 325, 340
TaqI TCGA 5 cut(s) 95, 110, 123, 495, 652
TasI AATT 4 cut(s) 264, 411, 455, 466
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 1 cut(s) 649
Tru1I TTAA 3 cut(s) 450, 462, 534
Tru9I TTAA 3 cut(s) 450, 462, 534
TscAI CASTG 5 cut(s) 75, 133, 300, 343, 407
TseFI GTSAC 3 cut(s) 128, 488, 560
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 3 cut(s) 128, 488, 560
TspDTI ATGAA 2 cut(s) 22, 459
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 5 cut(s) 75, 133, 300, 343, 407
Van91I CCANNNNNTGG 1 cut(s) 220
VneI GTGCAC 1 cut(s) 254
XapI RAATTY 1 cut(s) 466
XceI RCATGY 1 cut(s) 358
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.