FvH4_4g18470

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002435
Physical Location & Seq
Reverse (-)
22334659 .. 22336391
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18470.t1

Sequence Viewer

Length: 459 bp
ATGGCTTCCTTTCTCTCAAAGGCAATTCTCCTTCCCGTTGCGGCTTCCATTCACCGACGAGGTTACGCGGCGGCATCACATGGCTCAGTGGCAGCTGGCTTTGGAATGGGAGGCTCCAGGAGCACTATGGTGAGGAAGCTGGAAGAGAAGGTTGGGATGAAGGAAGAGTCTTCGTCTGCTTGGGCACCGGATCCTCGAACCGGGTACTACAGGCCGGCTAATAGGGCGGATGAGATCGACCCGGTGGAGCTCCGGCAAATGCTCTTGAACCAGAAAGGATCGATCTGTGTCTTAGGTCCGGTATTAAAGCTACTCAAAACCAATACGTACCTAACTTCGTCCTCAAAGTCTCAGACCTGCGCGCGGACTACCCAACGGTTAAGGAGAGCAGTAGTGGCCGAAGCACCACCAAAGTTTTCCAGTCTATGGGGGCCTATTCGCTGCCCTAACTCATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_3

Protein Analysis

153

Amino Acids

16.51

Weight (kDa)

10.13

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_3a PF03242 2 - 92 1.6e-27 Late embryogenesis abundant protein (LEA_3a subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 365
AccB1I GGYRCC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 426
AccII CGCG 3 cut(s) 68, 362, 364
AciI CCGC 5 cut(s) 41, 68, 71, 227, 364
AclWI GGATC 3 cut(s) 185, 198, 286
AcoI YGGCCR 1 cut(s) 396
AfaI GTAC 2 cut(s) 206, 329
AfiI CCNNNNNNNGG 3 cut(s) 200, 363, 426
AgsI TTSAA 1 cut(s) 268
AjnI CCWGG 1 cut(s) 116
AjuI GAANNNNNNNTTGG 2 cut(s) 135, 167
AleI CACNNNNGTG 1 cut(s) 128
AluBI AGCT 4 cut(s) 95, 139, 250, 310
AluI AGCT 4 cut(s) 95, 139, 250, 310
Alw21I GWGCWC 2 cut(s) 125, 252
Alw26I GTCTC 1 cut(s) 354
AlwI GGATC 3 cut(s) 185, 198, 286
AoxI GGCC 3 cut(s) 212, 396, 431
ApeKI GCWGC 2 cut(s) 92, 441
AspLEI GCGC 2 cut(s) 362, 364
AspS9I GGNCC 2 cut(s) 296, 431
AsuC2I CCSGG 2 cut(s) 202, 242
AsuHPI GGTGA 2 cut(s) 44, 142
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 187
BamHI GGATCC 1 cut(s) 190
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 2 cut(s) 125, 252
BbvI GCAGC 2 cut(s) 104, 428
BciT130I CCWGG 1 cut(s) 118
BcnI CCSGG 2 cut(s) 202, 242
BcoDI GTCTC 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 365
BisI GCNGC 5 cut(s) 42, 69, 72, 93, 442
BlsI GCNGC 5 cut(s) 43, 70, 73, 94, 443
Bme1390I CCNGG 3 cut(s) 118, 202, 242
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 296, 431
BmiI GGNNCC 4 cut(s) 115, 186, 192, 432
BmrFI CCNGG 3 cut(s) 118, 202, 242
BmsI GCATC 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 100
BpuMI CCSGG 2 cut(s) 202, 242
Bsa29I ATCGAT 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 327
BsaWI WCCGGW 2 cut(s) 187, 298
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 363, 426
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 1 cut(s) 420
BseBI CCWGG 1 cut(s) 118
BseCI ATCGAT 1 cut(s) 281
BseGI GGATG 2 cut(s) 162, 235
BseLI CCNNNNNNNGG 3 cut(s) 200, 363, 426
BseMII CTCAG 2 cut(s) 99, 365
BseNI ACTGG 1 cut(s) 420
BsePI GCGCGC 1 cut(s) 360
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 2 cut(s) 104, 428
Bsh1236I CGCG 3 cut(s) 68, 362, 364
BshFI GGCC 3 cut(s) 214, 398, 433
BshNI GGYRCC 1 cut(s) 184
BshVI ATCGAT 1 cut(s) 281
BsiHKAI GWGCWC 2 cut(s) 125, 252
BsiSI CCGG 6 cut(s) 188, 201, 215, 242, 253, 299
BslI CCNNNNNNNGG 3 cut(s) 200, 363, 426
BsmAI GTCTC 1 cut(s) 354
BsnI GGCC 3 cut(s) 214, 398, 433
Bsp1286I GDGCHC 3 cut(s) 125, 187, 252
Bsp143I GATC 4 cut(s) 190, 234, 278, 282
BspACI CCGC 5 cut(s) 41, 68, 71, 227, 364
BspANI GGCC 3 cut(s) 214, 398, 433
BspCNI CTCAG 2 cut(s) 98, 364
BspDI ATCGAT 1 cut(s) 281
BspFNI CGCG 3 cut(s) 68, 362, 364
BspLI GGNNCC 4 cut(s) 115, 186, 192, 432
BspMI ACCTGC 1 cut(s) 365
BspPI GGATC 3 cut(s) 185, 198, 286
BspT107I GGYRCC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 1 cut(s) 420
BssAI RCCGGY 1 cut(s) 214
BssHII GCGCGC 1 cut(s) 360
BssMI GATC 4 cut(s) 190, 234, 278, 282
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 378
Bst6I CTCTTC 2 cut(s) 138, 159
BstBAI YACGTR 1 cut(s) 327
BstC8I GCNNGC 3 cut(s) 97, 216, 362
BstDEI CTNAG 3 cut(s) 85, 292, 351
BstF5I GGATG 2 cut(s) 162, 235
BstFNI CGCG 3 cut(s) 68, 362, 364
BstHHI GCGC 2 cut(s) 362, 364
BstKTI GATC 4 cut(s) 193, 237, 281, 285
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 4 cut(s) 190, 234, 278, 282
BstMWI GCNNNNNNNGC 3 cut(s) 120, 224, 395
BstNI CCWGG 1 cut(s) 118
BstSCI CCNGG 3 cut(s) 116, 200, 240
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 187
BstSNI TACGTA 1 cut(s) 327
BstUI CGCG 3 cut(s) 68, 362, 364
BstV1I GCAGC 2 cut(s) 104, 428
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 281
BsuRI GGCC 3 cut(s) 214, 398, 433
BsuTUI ATCGAT 1 cut(s) 281
BtsCI GGATG 2 cut(s) 162, 235
BtsIMutI CAGTG 1 cut(s) 93
BveI ACCTGC 1 cut(s) 365
Cac8I GCNNGC 3 cut(s) 97, 216, 362
CfoI GCGC 2 cut(s) 362, 364
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 296, 431
ClaI ATCGAT 1 cut(s) 281
Csp6I GTAC 2 cut(s) 205, 328
CviAII CATG 1 cut(s) 80
CviQI GTAC 2 cut(s) 205, 328
DdeI CTNAG 3 cut(s) 85, 292, 351
DpnI GATC 4 cut(s) 192, 236, 280, 284
DpnII GATC 4 cut(s) 190, 234, 278, 282
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 2 cut(s) 138, 159
EarI CTCTTC 2 cut(s) 138, 159
EciI GGCGGA 1 cut(s) 242
Ecl136II GAGCTC 1 cut(s) 250
Eco105I TACGTA 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 116
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 1 cut(s) 83
FaiI YATR 4 cut(s) 81, 128, 427, 454
FatI CATG 1 cut(s) 79
Fnu4HI GCNGC 5 cut(s) 42, 69, 72, 93, 442
FokI GGATG 2 cut(s) 169, 242
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 5 cut(s) 42, 69, 72, 93, 442
GlaI GCGC 2 cut(s) 361, 363
GluI GCNGC 5 cut(s) 42, 69, 72, 93, 442
GsuI CTGGAG 1 cut(s) 100
HaeIII GGCC 3 cut(s) 214, 398, 433
HapII CCGG 6 cut(s) 188, 201, 215, 242, 253, 299
HhaI GCGC 2 cut(s) 362, 364
Hin1II CATG 1 cut(s) 83
Hin6I GCGC 2 cut(s) 360, 362
HinP1I GCGC 2 cut(s) 360, 362
HinfI GANTC 1 cut(s) 167
HpaII CCGG 6 cut(s) 188, 201, 215, 242, 253, 299
HphI GGTGA 2 cut(s) 44, 142
Hpy188I TCNGA 1 cut(s) 354
Hpy188III TCNNGA 1 cut(s) 265
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 3 cut(s) 41, 142, 154
HpyCH4III ACNGT 1 cut(s) 378
HpyCH4IV ACGT 1 cut(s) 326
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 224, 395
HpyF3I CTNAG 3 cut(s) 85, 292, 351
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 1 cut(s) 83
HspAI GCGC 2 cut(s) 360, 362
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 4 cut(s) 190, 234, 278, 282
LmnI GCTCC 4 cut(s) 119, 120, 247, 255
Lsp1109I GCAGC 2 cut(s) 104, 428
LweI GCATC 1 cut(s) 83
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 1 cut(s) 62
MalI GATC 4 cut(s) 192, 236, 280, 284
MboI GATC 4 cut(s) 190, 234, 278, 282
MboII GAAGA 3 cut(s) 155, 162, 176
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 3 cut(s) 125, 187, 252
MluCI AATT 1 cut(s) 24
MlyI GAGTC 1 cut(s) 176
MnlI CCTC 5 cut(s) 53, 104, 126, 204, 352
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 2 cut(s) 305, 380
MslI CAYNNNNRTG 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 6 cut(s) 188, 201, 215, 242, 253, 299
MspR9I CCNGG 3 cut(s) 118, 202, 242
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 3 cut(s) 68, 362, 364
MwoI GCNNNNNNNGC 3 cut(s) 120, 224, 395
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 2 cut(s) 202, 242
NdeII GATC 4 cut(s) 190, 234, 278, 282
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 1 cut(s) 83
NlaIV GGNNCC 4 cut(s) 115, 186, 192, 432
OliI CACNNNNGTG 1 cut(s) 128
PauI GCGCGC 1 cut(s) 360
PdiI GCCGGC 1 cut(s) 216
PflMI CCANNNNNTGG 1 cut(s) 426
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 5 cut(s) 43, 70, 73, 94, 443
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 327
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 4 cut(s) 115, 186, 192, 432
PspPI GGNCC 2 cut(s) 296, 431
PsuI RGATCY 1 cut(s) 190
PteI GCGCGC 1 cut(s) 360
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 2 cut(s) 206, 329
RsaNI GTAC 2 cut(s) 205, 328
RseI CAYNNNNRTG 1 cut(s) 128
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 305, 380
SatI GCNGC 5 cut(s) 42, 69, 72, 93, 442
Sau3AI GATC 4 cut(s) 190, 234, 278, 282
Sau96I GGNCC 2 cut(s) 296, 431
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 3 cut(s) 118, 202, 242
SduI GDGCHC 3 cut(s) 125, 187, 252
SfaNI GCATC 1 cut(s) 83
SfcI CTRYAG 1 cut(s) 208
SinI GGWCC 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 128
SnaBI TACGTA 1 cut(s) 327
Sse9I AATT 1 cut(s) 24
SsiI CCGC 5 cut(s) 41, 68, 71, 227, 364
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 116, 200, 240
TaaI ACNGT 1 cut(s) 378
TaiI ACGT 1 cut(s) 329
TaqI TCGA 3 cut(s) 196, 237, 281
TasI AATT 1 cut(s) 24
TauI GCSGC 3 cut(s) 44, 71, 74
Tru1I TTAA 2 cut(s) 305, 380
Tru9I TTAA 2 cut(s) 305, 380
TscAI CASTG 1 cut(s) 93
TseI GCWGC 2 cut(s) 92, 441
TspDTI ATGAA 1 cut(s) 173
TspRI CASTG 1 cut(s) 93
Van91I CCANNNNNTGG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 296
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.