Rroxscaffold_2G00099360

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002435
Physical Location & Seq
Reverse (-)
21025170 .. 21025768
599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099360.1

Sequence Viewer

Length: 294 bp
ATGGCCAAGGTTCCCATGAACCCGTTTCTCCTTCTCAGCAAGAGATGGTATAAGGTAGCTGCAGAGAGTACTAGTACTGAAAGAGTGCAGCCGGCAGTGTCAACAATGAGGAGGATGGCAACAACAGAATCCTCCGACAGCAGCGGTCGATCCATCGCAGATGATCAGAAGAAGAAGAAGAAAGTGTTTTGGATGAGAGACCCAAAGACGGGAAACTGGATTCCAGAGAATCACTTTGAAGAGATTGATGCTGCAGAGCTCAGGGAGAAGCTCCTCAACAAGCACAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_3

Protein Analysis

97

Amino Acids

11.24

Weight (kDa)

9.79

Isoelectric Point (pI)

58.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_3a PF03242 12 - 95 3.2e-18 Late embryogenesis abundant protein (LEA_3a subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 144
AclWI GGATC 1 cut(s) 144
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 2 cut(s) 70, 76
AfiI CCNNNNNNNGG 2 cut(s) 208, 209
AgsI TTSAA 1 cut(s) 239
AhlI ACTAGT 1 cut(s) 71
AluBI AGCT 3 cut(s) 59, 259, 271
AluI AGCT 3 cut(s) 59, 259, 271
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 192
AlwI GGATC 1 cut(s) 144
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 4 cut(s) 59, 88, 141, 251
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 46, 100, 153, 238
BccI CCATC 3 cut(s) 39, 109, 161
BclI TGATCA 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 192
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 2 cut(s) 60, 252
BisI GCNGC 4 cut(s) 60, 89, 142, 252
BlsI GCNGC 4 cut(s) 61, 90, 143, 253
BmcAI AGTACT 2 cut(s) 70, 76
BmiI GGNNCC 1 cut(s) 12
BmsI GCATC 1 cut(s) 238
Bpu10I CCTNAGC 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 6
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 209
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 2 cut(s) 120, 198
BseLI CCNNNNNNNGG 2 cut(s) 208, 209
BseMII CTCAG 2 cut(s) 49, 274
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 2 cut(s) 124, 263
BseXI GCAGC 4 cut(s) 46, 100, 153, 238
BsgI GTGCAG 1 cut(s) 107
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 208, 209
BsmAI GTCTC 1 cut(s) 192
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 2 cut(s) 149, 163
BspACI CCGC 1 cut(s) 144
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 48, 273
BspLI GGNNCC 1 cut(s) 12
BspMAI CTGCAG 2 cut(s) 64, 256
BspPI GGATC 1 cut(s) 144
BspTNI GGTCTC 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 2 cut(s) 149, 163
BssT1I CCWWGG 1 cut(s) 6
Bst6I CTCTTC 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 35, 260
BstF5I GGATG 2 cut(s) 120, 198
BstKTI GATC 2 cut(s) 152, 166
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 2 cut(s) 149, 163
BstMCI CGRYCG 1 cut(s) 148
BstSFI CTRYAG 2 cut(s) 60, 252
BstV1I GCAGC 4 cut(s) 46, 100, 153, 238
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 2 cut(s) 120, 198
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 102
Cac8I GCNNGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 91
Csp6I GTAC 2 cut(s) 69, 75
CviAII CATG 1 cut(s) 16
CviJI RGCY 5 cut(s) 5, 59, 91, 259, 271
CviKI_1 RGCY 5 cut(s) 5, 59, 91, 259, 271
CviQI GTAC 2 cut(s) 69, 75
DdeI CTNAG 2 cut(s) 35, 260
DpnI GATC 2 cut(s) 151, 165
DpnII GATC 2 cut(s) 149, 163
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 1 cut(s) 19
FaiI YATR 2 cut(s) 17, 51
FatI CATG 1 cut(s) 15
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 4 cut(s) 60, 89, 142, 252
FokI GGATG 2 cut(s) 127, 205
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 4 cut(s) 60, 89, 142, 252
FspBI CTAG 1 cut(s) 72
GluI GCNGC 4 cut(s) 60, 89, 142, 252
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 92
Hin1II CATG 1 cut(s) 19
HincII GTYRAC 1 cut(s) 102
HindII GTYRAC 1 cut(s) 102
HinfI GANTC 3 cut(s) 128, 220, 229
HpaII CCGG 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 102
Hpy188I TCNGA 2 cut(s) 136, 168
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 1 cut(s) 102
HpyAV CCTTC 1 cut(s) 41
HpyCH4V TGCA 3 cut(s) 62, 88, 254
HpyF3I CTNAG 2 cut(s) 35, 260
Hsp92II CATG 1 cut(s) 19
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 2 cut(s) 149, 163
LmnI GCTCC 1 cut(s) 276
LpnPI CCDG 4 cut(s) 105, 202, 237, 247
Lsp1109I GCAGC 4 cut(s) 46, 100, 153, 238
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 72
MalI GATC 2 cut(s) 151, 165
MboI GATC 2 cut(s) 149, 163
MboII GAAGA 5 cut(s) 181, 184, 187, 190, 251
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 4 cut(s) 102, 105, 142, 284
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 91
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 292
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 144
MspI CCGG 1 cut(s) 92
NaeI GCCGGC 1 cut(s) 93
NdeII GATC 2 cut(s) 149, 163
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 1 cut(s) 19
NlaIV GGNNCC 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 128, 220, 229
PkrI GCNGC 4 cut(s) 61, 90, 143, 253
Psp124BI GAGCTC 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 12
PstI CTGCAG 2 cut(s) 64, 256
RsaI GTAC 2 cut(s) 70, 76
RsaNI GTAC 2 cut(s) 69, 75
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 1 cut(s) 292
SatI GCNGC 4 cut(s) 60, 89, 142, 252
Sau3AI GATC 2 cut(s) 149, 163
ScaI AGTACT 2 cut(s) 70, 76
SduI GDGCHC 1 cut(s) 261
SetI ASST 6 cut(s) 12, 57, 61, 261, 273, 291
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 2 cut(s) 60, 252
SpeI ACTAGT 1 cut(s) 71
SsiI CCGC 1 cut(s) 144
SspMI CTAG 1 cut(s) 72
SstI GAGCTC 1 cut(s) 261
StyI CCWWGG 1 cut(s) 6
TaqI TCGA 1 cut(s) 148
TatI WGTACW 2 cut(s) 68, 74
TfiI GAWTC 3 cut(s) 128, 220, 229
Tru1I TTAA 1 cut(s) 292
Tru9I TTAA 1 cut(s) 292
TscAI CASTG 1 cut(s) 102
TseI GCWGC 4 cut(s) 59, 88, 141, 251
TspDTI ATGAA 1 cut(s) 32
TspRI CASTG 1 cut(s) 102
XspI CTAG 1 cut(s) 72
ZrmI AGTACT 2 cut(s) 70, 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.