MD00G1127700.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002435
Physical Location & Seq
Reverse (-)
27171624 .. 27172338
715 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1127700.v1.1.491

Sequence Viewer

Length: 294 bp
ATGGCTGCTTCCTTGCTCTCCAAAACTAAGCCCCTCCTCCACGCGATCCAACGGCGGGGTTACGCTGCAGCATCACATGGTTCGGTGGCAGCTGGATTCGGTAGGGGAGGGTCCAGGAATGCTATGGTGGAGGAGCGGGTTGCGAAAGCGATGAAGGAGGAGTCTGGACTCGGAGCTCCTTCATCTGCGTGGGCCCCGGATCCGAGAACTGGATACTATAGGCCCGCCAATCGTGCGGACGAGATCGACCCGGTTGGGCTTCGAGAGATGCTGTTGAACCACAAAGCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_3

Protein Analysis

98

Amino Acids

10.33

Weight (kDa)

10.08

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_3a PF03242 12 - 95 2e-24 Late embryogenesis abundant protein (LEA_3a subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 1 cut(s) 44
AciI CCGC 4 cut(s) 55, 136, 225, 236
AclWI GGATC 3 cut(s) 40, 194, 207
AfiI CCNNNNNNNGG 2 cut(s) 55, 209
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 1 cut(s) 113
AluBI AGCT 2 cut(s) 92, 176
AluI AGCT 2 cut(s) 92, 176
Alw21I GWGCWC 1 cut(s) 178
AlwI GGATC 3 cut(s) 40, 194, 207
AoxI GGCC 2 cut(s) 192, 221
ApaI GGGCCC 1 cut(s) 196
ApeKI GCWGC 4 cut(s) 5, 65, 68, 89
AspS9I GGNCC 4 cut(s) 111, 192, 193, 222
AsuC2I CCSGG 2 cut(s) 197, 251
AvaII GGWCC 1 cut(s) 111
BaeGI GKGCMC 1 cut(s) 196
BamHI GGATCC 1 cut(s) 199
BanII GRGCYC 2 cut(s) 178, 196
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 52, 80, 101
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 115
BciVI GTATCC 1 cut(s) 206
BcnI CCSGG 2 cut(s) 197, 251
BfmI CTRYAG 2 cut(s) 66, 217
BfuI GTATCC 1 cut(s) 206
BisI GCNGC 4 cut(s) 6, 66, 69, 90
BlsI GCNGC 4 cut(s) 7, 67, 70, 91
Bme1390I CCNGG 3 cut(s) 115, 197, 251
Bme18I GGWCC 1 cut(s) 111
BmgT120I GGNCC 4 cut(s) 111, 192, 193, 222
BmiI GGNNCC 4 cut(s) 112, 194, 195, 201
BmrFI CCNGG 3 cut(s) 115, 197, 251
BmsI GCATC 2 cut(s) 80, 258
BpuMI CCSGG 2 cut(s) 197, 251
BsaJI CCNNGG 1 cut(s) 195
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 209
Bse1I ACTGG 1 cut(s) 214
BseBI CCWGG 1 cut(s) 115
BseDI CCNNGG 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 55, 209
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 3 cut(s) 26, 146, 173
BseSI GKGCMC 1 cut(s) 196
BseXI GCAGC 3 cut(s) 52, 80, 101
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 2 cut(s) 194, 223
BsiHKAI GWGCWC 1 cut(s) 178
BsiSI CCGG 2 cut(s) 197, 251
BslI CCNNNNNNNGG 2 cut(s) 55, 209
BsmI GAATGC 1 cut(s) 124
BsnI GGCC 2 cut(s) 194, 223
Bsp120I GGGCCC 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 178, 196
Bsp143I GATC 3 cut(s) 45, 199, 243
BspACI CCGC 4 cut(s) 55, 136, 225, 236
BspANI GGCC 2 cut(s) 194, 223
BspFNI CGCG 1 cut(s) 44
BspLI GGNNCC 4 cut(s) 112, 194, 195, 201
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 3 cut(s) 40, 194, 207
BsrBI CCGCTC 1 cut(s) 136
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 3 cut(s) 45, 199, 243
Bst2UI CCWGG 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 1 cut(s) 27
BstFNI CGCG 1 cut(s) 44
BstKTI GATC 3 cut(s) 48, 202, 246
BstMBI GATC 3 cut(s) 45, 199, 243
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNI CCWGG 1 cut(s) 115
BstSCI CCNGG 3 cut(s) 113, 195, 249
BstSFI CTRYAG 2 cut(s) 66, 217
BstSLI GKGCMC 1 cut(s) 196
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 3 cut(s) 52, 80, 101
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
BsuI GTATCC 1 cut(s) 206
BsuRI GGCC 2 cut(s) 194, 223
BtgZI GCGATG 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 225
Cfr13I GGNCC 4 cut(s) 111, 192, 193, 222
CviAII CATG 1 cut(s) 77
CviJI RGCY 7 cut(s) 5, 31, 92, 176, 194, 223, 259
CviKI_1 RGCY 7 cut(s) 5, 31, 92, 176, 194, 223, 259
DdeI CTNAG 1 cut(s) 27
DpnI GATC 3 cut(s) 47, 201, 245
DpnII GATC 3 cut(s) 45, 199, 243
Ecl136II GAGCTC 1 cut(s) 176
Eco24I GRGCYC 2 cut(s) 178, 196
Eco47I GGWCC 1 cut(s) 111
Eco53kI GAGCTC 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 113
EcoT38I GRGCYC 2 cut(s) 178, 196
FaeI CATG 1 cut(s) 80
FaiI YATR 3 cut(s) 78, 125, 219
FatI CATG 1 cut(s) 76
FauI CCCGC 3 cut(s) 48, 129, 232
Fnu4HI GCNGC 4 cut(s) 6, 66, 69, 90
FriOI GRGCYC 2 cut(s) 178, 196
Fsp4HI GCNGC 4 cut(s) 6, 66, 69, 90
GluI GCNGC 4 cut(s) 6, 66, 69, 90
HaeIII GGCC 2 cut(s) 194, 223
HapII CCGG 2 cut(s) 197, 251
Hin1II CATG 1 cut(s) 80
HinfI GANTC 3 cut(s) 96, 161, 168
HpaII CCGG 2 cut(s) 197, 251
Hpy188I TCNGA 2 cut(s) 173, 204
Hpy188III TCNNGA 2 cut(s) 165, 263
HpyAV CCTTC 2 cut(s) 148, 189
HpyCH4V TGCA 1 cut(s) 68
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 1 cut(s) 27
Hsp92II CATG 1 cut(s) 80
Kzo9I GATC 3 cut(s) 45, 199, 243
LmnI GCTCC 3 cut(s) 133, 173, 181
LpnPI CCDG 7 cut(s) 78, 100, 127, 150, 195, 210, 264
Lsp1109I GCAGC 3 cut(s) 52, 80, 101
LweI GCATC 2 cut(s) 80, 258
MaeIII GTNAC 1 cut(s) 59
MalI GATC 3 cut(s) 47, 201, 245
MbiI CCGCTC 1 cut(s) 136
MboI GATC 3 cut(s) 45, 199, 243
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 2 cut(s) 178, 196
MlyI GAGTC 2 cut(s) 162, 170
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 5 cut(s) 44, 47, 101, 124, 151
MslI CAYNNNNRTG 1 cut(s) 187
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 197, 251
MspR9I CCNGG 3 cut(s) 115, 197, 251
Mva1269I GAATGC 1 cut(s) 124
MvaI CCWGG 1 cut(s) 115
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 1 cut(s) 233
NciI CCSGG 2 cut(s) 197, 251
NdeII GATC 3 cut(s) 45, 199, 243
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 4 cut(s) 112, 194, 195, 201
PctI GAATGC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 96
PfoI TCCNGGA 1 cut(s) 113
PkrI GCNGC 4 cut(s) 7, 67, 70, 91
PleI GAGTC 2 cut(s) 162, 169
PpsI GAGTC 2 cut(s) 162, 169
Psp124BI GAGCTC 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 113
PspGI CCWGG 1 cut(s) 113
PspN4I GGNNCC 4 cut(s) 112, 194, 195, 201
PspOMI GGGCCC 1 cut(s) 192
PspPI GGNCC 4 cut(s) 111, 192, 193, 222
PstI CTGCAG 1 cut(s) 70
PsuI RGATCY 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 92
RseI CAYNNNNRTG 1 cut(s) 187
SacI GAGCTC 1 cut(s) 178
SatI GCNGC 4 cut(s) 6, 66, 69, 90
Sau3AI GATC 3 cut(s) 45, 199, 243
Sau96I GGNCC 4 cut(s) 111, 192, 193, 222
SchI GAGTC 2 cut(s) 162, 170
ScrFI CCNGG 3 cut(s) 115, 197, 251
SduI GDGCHC 2 cut(s) 178, 196
SetI ASST 2 cut(s) 94, 178
SfaNI GCATC 2 cut(s) 80, 258
SfcI CTRYAG 2 cut(s) 66, 217
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 187
SsiI CCGC 4 cut(s) 55, 136, 225, 236
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 3 cut(s) 113, 195, 249
TaqI TCGA 2 cut(s) 246, 262
TfiI GAWTC 1 cut(s) 96
TseI GCWGC 4 cut(s) 5, 65, 68, 89
TspDTI ATGAA 2 cut(s) 167, 171
VpaK11BI GGWCC 1 cut(s) 111
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.