Rh2CG446000

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002435
Physical Location & Seq
Forward (+)
60390130 .. 60403261
13132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG446000.1

Sequence Viewer

Length: 480 bp
ATGGAGTTAACGCTGAATGAAACATTTGAAAATAAATGGTCTAAGTTGGACTTATATAGTAAAAGCGGGCACCCACTAATAACAATGATTCGACAGCATCACAACTCAAAAAAGACCAAGGGGCACAATATCATTGAAGAAGTGAAGCCACCGACTCTAGACATTTCAATTAATGGCATTTTTTTTGTCATGGCCAAGGTTCCCATGAACCCGTTTCTCCTTCTCAGCAAGAGATGGTATAAGGTAGCTGCAGAGAGTACTAGTACTGAAAGAGTGCAGCCGGCAGTGTCAACAATGAGGAGGATGGCAACAACAGAATCCTCCGGCAGCAGCGGTCGAGCCGTCGCAGATGATCAGGAGAAGAAGAAAGTGTTTTGGATGAGAGACCCAAAGACGGGGAACTGGATTCCAGAGAATCACTTTGAAGAGATTGATGCTGCAGAGCTCAGGGAGAAGCTCCTCAACAAGCACAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_3

Protein Analysis

159

Amino Acids

18.32

Weight (kDa)

9.42

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_3a PF03242 75 - 157 8.3e-18 Late embryogenesis abundant protein (LEA_3a subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 69
AciI CCGC 2 cut(s) 66, 333
AcoI YGGCCR 1 cut(s) 192
AfaI GTAC 2 cut(s) 259, 265
AfiI CCNNNNNNNGG 2 cut(s) 394, 395
AgsI TTSAA 4 cut(s) 29, 137, 168, 425
AhlI ACTAGT 1 cut(s) 260
AluBI AGCT 3 cut(s) 248, 445, 457
AluI AGCT 3 cut(s) 248, 445, 457
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 1 cut(s) 378
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 5 cut(s) 248, 277, 327, 330, 437
AseI ATTAAT 1 cut(s) 171
BaeGI GKGCMC 2 cut(s) 72, 126
BalI TGGCCA 1 cut(s) 194
BanI GGYRCC 1 cut(s) 69
BanII GRGCYC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 5 cut(s) 235, 289, 339, 342, 424
BccI CCATC 2 cut(s) 228, 298
BceAI ACGGC 1 cut(s) 326
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 378
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 2 cut(s) 158, 261
BfmI CTRYAG 2 cut(s) 249, 438
BisI GCNGC 5 cut(s) 249, 278, 328, 331, 438
BlsI GCNGC 5 cut(s) 250, 279, 329, 332, 439
BmcAI AGTACT 2 cut(s) 259, 265
BmiI GGNNCC 2 cut(s) 71, 201
BmsI GCATC 2 cut(s) 106, 424
Bpu10I CCTNAGC 1 cut(s) 446
BsaI GGTCTC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 117, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 395
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 407
BseDI CCNNGG 2 cut(s) 117, 195
BseGI GGATG 2 cut(s) 309, 384
BseLI CCNNNNNNNGG 2 cut(s) 394, 395
BseMII CTCAG 2 cut(s) 238, 460
BseNI ACTGG 1 cut(s) 407
BseRI GAGGAG 2 cut(s) 313, 449
BseSI GKGCMC 2 cut(s) 72, 126
BseXI GCAGC 5 cut(s) 235, 289, 339, 342, 424
BsgI GTGCAG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 69
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 447
BsiSI CCGG 2 cut(s) 281, 324
BslI CCNNNNNNNGG 2 cut(s) 394, 395
BsmAI GTCTC 1 cut(s) 378
BsnI GGCC 1 cut(s) 194
Bso31I GGTCTC 1 cut(s) 378
Bsp1286I GDGCHC 3 cut(s) 72, 126, 447
Bsp143I GATC 1 cut(s) 352
BspACI CCGC 2 cut(s) 66, 333
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 2 cut(s) 237, 459
BspLI GGNNCC 2 cut(s) 71, 201
BspMAI CTGCAG 2 cut(s) 253, 442
BspT107I GGYRCC 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 378
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 117, 195
BssMI GATC 1 cut(s) 352
BssT1I CCWWGG 2 cut(s) 117, 195
Bst6I CTCTTC 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 68, 282
BstDEI CTNAG 3 cut(s) 42, 224, 446
BstF5I GGATG 2 cut(s) 309, 384
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 1 cut(s) 352
BstMCI CGRYCG 1 cut(s) 337
BstSFI CTRYAG 2 cut(s) 249, 438
BstSLI GKGCMC 2 cut(s) 72, 126
BstV1I GCAGC 5 cut(s) 235, 289, 339, 342, 424
BsuRI GGCC 1 cut(s) 194
BtsCI GGATG 2 cut(s) 309, 384
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 291
Cac8I GCNNGC 2 cut(s) 68, 282
Cfr10I RCCGGY 1 cut(s) 280
Csp6I GTAC 2 cut(s) 258, 264
CviAII CATG 2 cut(s) 190, 205
CviJI RGCY 7 cut(s) 148, 194, 248, 280, 341, 445, 457
CviKI_1 RGCY 7 cut(s) 148, 194, 248, 280, 341, 445, 457
CviQI GTAC 2 cut(s) 258, 264
DdeI CTNAG 3 cut(s) 42, 224, 446
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 192
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Ecl136II GAGCTC 1 cut(s) 445
Eco130I CCWWGG 2 cut(s) 117, 195
Eco24I GRGCYC 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 445
EcoICRI GAGCTC 1 cut(s) 445
EcoT14I CCWWGG 2 cut(s) 117, 195
EcoT38I GRGCYC 1 cut(s) 447
ErhI CCWWGG 2 cut(s) 117, 195
FaeI CATG 2 cut(s) 193, 208
FaiI YATR 5 cut(s) 55, 57, 191, 206, 240
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FatI CATG 2 cut(s) 189, 204
FauI CCCGC 1 cut(s) 59
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 5 cut(s) 249, 278, 328, 331, 438
FokI GGATG 2 cut(s) 316, 391
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 5 cut(s) 249, 278, 328, 331, 438
FspBI CTAG 2 cut(s) 158, 261
GluI GCNGC 5 cut(s) 249, 278, 328, 331, 438
HaeIII GGCC 1 cut(s) 194
HapII CCGG 2 cut(s) 281, 324
Hin1II CATG 2 cut(s) 193, 208
HincII GTYRAC 2 cut(s) 9, 291
HindII GTYRAC 2 cut(s) 9, 291
HinfI GANTC 5 cut(s) 88, 154, 317, 406, 415
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 2 cut(s) 281, 324
Hpy166II GTNNAC 2 cut(s) 9, 291
Hpy188III TCNNGA 3 cut(s) 158, 356, 410
Hpy8I GTNNAC 2 cut(s) 9, 291
Hpy99I CGWCG 1 cut(s) 347
HpyAV CCTTC 1 cut(s) 230
HpyCH4V TGCA 3 cut(s) 251, 277, 440
HpyF3I CTNAG 3 cut(s) 42, 224, 446
Hsp92II CATG 2 cut(s) 193, 208
KroI GCCGGC 1 cut(s) 280
KroNI GCCGGC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 352
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 462
LpnPI CCDG 6 cut(s) 294, 337, 341, 388, 423, 433
Lsp1109I GCAGC 5 cut(s) 235, 289, 339, 342, 424
LweI GCATC 2 cut(s) 106, 424
MaeI CTAG 2 cut(s) 158, 261
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 4 cut(s) 149, 373, 376, 437
MhlI GDGCHC 3 cut(s) 72, 126, 447
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 1 cut(s) 168
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 148
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 4 cut(s) 291, 294, 331, 470
Mox20I TGGCCA 1 cut(s) 194
MroNI GCCGGC 1 cut(s) 280
MscI TGGCCA 1 cut(s) 194
MseI TTAA 3 cut(s) 8, 171, 478
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 333
MspI CCGG 2 cut(s) 281, 324
NaeI GCCGGC 1 cut(s) 282
NdeII GATC 1 cut(s) 352
NgoMIV GCCGGC 1 cut(s) 280
NlaIII CATG 2 cut(s) 193, 208
NlaIV GGNNCC 2 cut(s) 71, 201
PdiI GCCGGC 1 cut(s) 282
PfeI GAWTC 4 cut(s) 88, 317, 406, 415
PkrI GCNGC 5 cut(s) 250, 279, 329, 332, 439
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PshBI ATTAAT 1 cut(s) 171
Psp124BI GAGCTC 1 cut(s) 447
PspN4I GGNNCC 2 cut(s) 71, 201
PstI CTGCAG 2 cut(s) 253, 442
RsaI GTAC 2 cut(s) 259, 265
RsaNI GTAC 2 cut(s) 258, 264
SacI GAGCTC 1 cut(s) 447
SaqAI TTAA 3 cut(s) 8, 171, 478
SatI GCNGC 5 cut(s) 249, 278, 328, 331, 438
Sau3AI GATC 1 cut(s) 352
ScaI AGTACT 2 cut(s) 259, 265
SchI GAGTC 1 cut(s) 148
SduI GDGCHC 3 cut(s) 72, 126, 447
SetI ASST 6 cut(s) 201, 246, 250, 447, 459, 477
SfaNI GCATC 2 cut(s) 106, 424
SfcI CTRYAG 2 cut(s) 249, 438
SpeI ACTAGT 1 cut(s) 260
Sse9I AATT 1 cut(s) 168
SsiI CCGC 2 cut(s) 66, 333
SspMI CTAG 2 cut(s) 158, 261
SstI GAGCTC 1 cut(s) 447
StyI CCWWGG 2 cut(s) 117, 195
TaqI TCGA 2 cut(s) 91, 337
TasI AATT 1 cut(s) 168
TatI WGTACW 2 cut(s) 257, 263
TfiI GAWTC 4 cut(s) 88, 317, 406, 415
Tru1I TTAA 3 cut(s) 8, 171, 478
Tru9I TTAA 3 cut(s) 8, 171, 478
TscAI CASTG 1 cut(s) 291
TseI GCWGC 5 cut(s) 248, 277, 327, 330, 437
TspDTI ATGAA 2 cut(s) 33, 221
TspRI CASTG 1 cut(s) 291
VspI ATTAAT 1 cut(s) 171
XbaI TCTAGA 1 cut(s) 157
XspI CTAG 2 cut(s) 158, 261
ZrmI AGTACT 2 cut(s) 259, 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.