FvH4_4g29290

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000995
Physical Location & Seq
Reverse (-)
29473935 .. 29479500
5566 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29290.t2

Sequence Viewer

Length: 1983 bp
ATGAAACTTGAGAGGGAACTGGTGTTTGGCCAATTAAGCCACAAGCCTGATTTTAAATCTTTTGATCACAACGAGATTAATCAGAATTCCCGGGGATTAGAATCAGCGAATTGGATTGGAAAGGAAAGTCAGAACAGAGTTTTGTTTGATTCGAAGGACAATGGGGAAGATGCTGGTCAAATTGATGCACTTGACTTGACTGCGACAAACTTTGATTGTTCAAAGAGTGTGGATGATTTGAAAACCTGTGATGAAGGTGAAATCAAAGATTTTGTGCCTGAAATATCTTTAGAGAAGCAATCAGATTATTATATGGACAAAAGTGTTACAGAATACAACTTGCCAGAACTGATAGTGTGTTACAAAGAGAGTAGTTGCAATACTATCAAGGACATCTGCATTGATGAGGGAGTGCCTTCCCAAGAGAAGATCTGGTTTGAGACTAACATGGATGAGAAAGCATTTTGCAATTATTTGTCCCCTGATGAGGTTCAGAAAAAGCAACCGCTTCAACAACAAATGGACATTGTTATGACCATTCCAGAAGATTTCAAGTCTTCTGCACAAAAATATGACGTAAAGGAGGATTTTGTCATTTCTAGTGATGCCAAGGATATGGTGCAGAGAGGGGAGGCGATGTATGATGCATCAGAGAAAACTGCAATTGATGTTTCCAGAGACATATTTTCTCCTGAAAACGAGTTGTCAACGCATGACTTGTGCACACAGAACTTGAACTCCAAGTCTTCTAGTAAGGCTAGCAGTGAAGCTGACCAAGATACTGTTCAGATGTCGACTGACAAGGAAAATGAAATAGCACAAACTCACTGTAGTCCTCTGGTTTCAGCAACGGGGGAATCACTCTTGAAGCTAGATCCTGTTCCTTCAGCTATAGAACTAAATTGCCATGTCGATGAGTTATCAGACAGTAGTAAAGTGGAGAATGGAAGCATTGCTGTTGGTGTGGACCGTTCGGCACCTGTATCGATTAGTAGATTCCCAAACCTGGATCCTGTCTCTTCCACTGAACTATCAAACTGTCTTATTGATGAGTTGTCCAACAATGTTAGAGCAGACAAAGGAGGAAGCAGTAATTGTGGTTTGAACTGTTCAGACATTACCGAGGATGTCTGTACAGAAAATGGGTTCAGCAAACATCTTGAAACTCAAAATATGGCTGAAGTTGATAGTGATGACATATGTGAATCAAAAATCATTTCAAGCAAAGTTCTGCCTTGTTTAGCACCTGTGACTACTGGCAGAGAGGATTGTCCTGAAAATGGTGCTTGTCAACGTCCTGAAACTTCAGATACATCTATGGTTGATGAGGACATTTCTGATTCAGAGATTGTTTCTAGCCAAGTACAGCATTCTTTAGTCGCTTCGACTATTGCCAAAGACGAATGTCCTAAAGATGGGGATCATAATGCACAGAATGTTCCGATCCAAGTTGAACGTGAGCTGGGAGAGTCTGGGGATCATAATGCACAGAATGTTCCGATCCAAGTTGAACGTGAGCTGGGAGAGTCTAGTTTCTCTGCAGGAATTCCTTTCGCGACTCTTATAAATTATAAGGAATCAATACCTTATTCAGGTACACACAATGCTTCGAGTGAAGTGCAACATGATCAAGGGGAATCAAGTTTTTCAGCAGTAGGTAACTCATCGAGTCTTATAAATAGTTCGGGACCAATACCTTATTCTGATTCAGAAAGATTCCTTGTTCAACGTGATCTGGGAGATTCGAGTTTTGCTGCAGTAGGTCATGTCTCAAGTCTTATAAATAGTTCTGGACCAATACCTTATTCTGGCAGTATCTCCCTTCGATCAGATAGCAGTGCAACCAGCACCCGCTCATTTGCCTTCCCTGTTATGCATCCGCATGAACCATTCAGCAGTCCAGTGAGAATGGCAAAAGCTGACCGGAGGCTTTTGCGTAAACATAGGGGTTGGAAGCGGGGTATTTTCTGCTGTAGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

661

Amino Acids

72.37

Weight (kDa)

4.51

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1565, 1572, 1676, 1781
AccB1I GGYRCC 1 cut(s) 976
AccBSI CCGCTC 1 cut(s) 1854
AccI GTMKAC 1 cut(s) 794
AccII CGCG 1 cut(s) 1556
AciI CCGC 4 cut(s) 506, 1852, 1880, 1957
AclWI GGATC 7 cut(s) 869, 1004, 1017, 1428, 1438, 1485, 1495
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 2 cut(s) 85, 1545
AcuI CTGAAG 3 cut(s) 870, 1200, 1290
AfaI GTAC 3 cut(s) 1135, 1365, 1597
AfiI CCNNNNNNNGG 6 cut(s) 487, 1006, 1280, 1415, 1592, 1808
AjnI CCWGG 1 cut(s) 1005
AjuI GAANNNNNNNTTGG 8 cut(s) 9, 41, 768, 800, 1684, 1716, 1789, 1821
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
AluBI AGCT 6 cut(s) 770, 871, 890, 1462, 1519, 1919
AluI AGCT 6 cut(s) 770, 871, 890, 1462, 1519, 1919
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 4 cut(s) 434, 672, 1021, 1774
Alw44I GTGCAC 1 cut(s) 721
AlwI GGATC 7 cut(s) 869, 1004, 1017, 1428, 1438, 1485, 1495
Ama87I CYCGRG 1 cut(s) 90
AoxI GGCC 1 cut(s) 28
ApaLI GTGCAC 1 cut(s) 721
ApeKI GCWGC 1 cut(s) 1754
ApoI RAATTY 2 cut(s) 85, 1545
AseI ATTAAT 1 cut(s) 78
AspS9I GGNCC 3 cut(s) 967, 1688, 1793
AsuC2I CCSGG 2 cut(s) 91, 92
AsuHPI GGTGA 1 cut(s) 269
AsuII TTCGAA 1 cut(s) 152
AsuNHI GCTAGC 1 cut(s) 758
AvaI CYCGRG 1 cut(s) 90
AvaII GGWCC 3 cut(s) 967, 1688, 1793
BaeGI GKGCMC 1 cut(s) 725
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 1009
BanI GGYRCC 1 cut(s) 976
BbsI GAAGAC 2 cut(s) 549, 738
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 1 cut(s) 1741
BccI CCATC 1 cut(s) 1409
BcgI CGANNNNNNTGC 4 cut(s) 966, 1000, 1600, 1634
BciT130I CCWGG 1 cut(s) 1007
BclI TGATCA 2 cut(s) 64, 1627
BcnI CCSGG 2 cut(s) 91, 92
BcoDI GTCTC 4 cut(s) 434, 672, 1021, 1774
BfaI CTAG 6 cut(s) 600, 750, 759, 872, 1356, 1530
BfmI CTRYAG 5 cut(s) 829, 891, 1539, 1755, 1972
BglII AGATCT 1 cut(s) 429
BisI GCNGC 1 cut(s) 1755
BlsI GCNGC 1 cut(s) 1756
Bme1390I CCNGG 3 cut(s) 91, 92, 1007
Bme18I GGWCC 3 cut(s) 967, 1688, 1793
BmeT110I CYCGRG 1 cut(s) 90
BmgT120I GGNCC 3 cut(s) 967, 1688, 1793
BmiI GGNNCC 3 cut(s) 978, 1011, 1689
BmrFI CCNGG 3 cut(s) 91, 92, 1007
BmsI GCATC 6 cut(s) 160, 175, 595, 634, 656, 1885
BmtI GCTAGC 1 cut(s) 762
BoxI GACNNNNGTC 1 cut(s) 1404
BpiI GAAGAC 2 cut(s) 549, 738
Bpu14I TTCGAA 1 cut(s) 152
BpuEI CTTGAG 2 cut(s) 29, 1756
BpuMI CCSGG 2 cut(s) 91, 92
Bsa29I ATCGAT 1 cut(s) 986
BsaBI GATNNNNATC 2 cut(s) 100, 1419
BsaJI CCNNGG 4 cut(s) 90, 91, 609, 1122
BsaWI WCCGGW 1 cut(s) 1923
BsaXI ACNNNNNCTCC 4 cut(s) 623, 653, 722, 752
Bsc4I CCNNNNNNNGG 6 cut(s) 487, 1006, 1280, 1415, 1592, 1808
Bse1I ACTGG 3 cut(s) 24, 1261, 1901
Bse3DI GCAATG 1 cut(s) 951
Bse8I GATNNNNATC 2 cut(s) 100, 1419
BseBI CCWGG 1 cut(s) 1007
BseCI ATCGAT 1 cut(s) 986
BseDI CCNNGG 4 cut(s) 90, 91, 609, 1122
BseGI GGATG 4 cut(s) 238, 457, 1132, 1876
BseJI GATNNNNATC 2 cut(s) 100, 1419
BseLI CCNNNNNNNGG 6 cut(s) 487, 1006, 1280, 1415, 1592, 1808
BseMI GCAATG 1 cut(s) 951
BseNI ACTGG 3 cut(s) 24, 1261, 1901
BseSI GKGCMC 1 cut(s) 725
BseXI GCAGC 1 cut(s) 1741
BseYI CCCAGC 2 cut(s) 1462, 1519
BsgI GTGCAG 2 cut(s) 546, 641
Bsh1236I CGCG 1 cut(s) 1556
BshFI GGCC 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 976
BshVI ATCGAT 1 cut(s) 986
BsiHKAI GWGCWC 1 cut(s) 725
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 2 cut(s) 91, 1924
BslFI GGGAC 2 cut(s) 463, 1701
BslI CCNNNNNNNGG 6 cut(s) 487, 1006, 1280, 1415, 1592, 1808
BsmAI GTCTC 4 cut(s) 434, 672, 1021, 1774
BsmFI GGGAC 2 cut(s) 463, 1701
BsmI GAATGC 1 cut(s) 1369
BsnI GGCC 1 cut(s) 30
BsoBI CYCGRG 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 725
Bsp1407I TGTACA 1 cut(s) 1133
Bsp68I TCGCGA 1 cut(s) 1556
BspACI CCGC 4 cut(s) 506, 1852, 1880, 1957
BspANI GGCC 1 cut(s) 30
BspDI ATCGAT 1 cut(s) 986
BspFNI CGCG 1 cut(s) 1556
BspLI GGNNCC 3 cut(s) 978, 1011, 1689
BspMAI CTGCAG 2 cut(s) 1543, 1759
BspOI GCTAGC 1 cut(s) 762
BspPI GGATC 7 cut(s) 869, 1004, 1017, 1428, 1438, 1485, 1495
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 976
BsrBI CCGCTC 1 cut(s) 1854
BsrDI GCAATG 1 cut(s) 951
BsrGI TGTACA 1 cut(s) 1133
BsrI ACTGG 3 cut(s) 24, 1261, 1901
BssECI CCNNGG 4 cut(s) 90, 91, 609, 1122
BssT1I CCWWGG 1 cut(s) 609
Bst2UI CCWGG 1 cut(s) 1007
Bst4CI ACNGT 6 cut(s) 784, 830, 929, 971, 1040, 1109
Bst6I CTCTTC 1 cut(s) 1024
BstAUI TGTACA 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 760
BstENI CCTNNNNNAGG 1 cut(s) 1590
BstF5I GGATG 4 cut(s) 238, 457, 1132, 1876
BstFNI CGCG 1 cut(s) 1556
BstMAI GTCTC 4 cut(s) 434, 672, 1021, 1774
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstNI CCWGG 1 cut(s) 1007
BstPAI GACNNNNGTC 1 cut(s) 1404
BstSCI CCNGG 3 cut(s) 89, 90, 1005
BstSFI CTRYAG 5 cut(s) 829, 891, 1539, 1755, 1972
BstSLI GKGCMC 1 cut(s) 725
BstUI CGCG 1 cut(s) 1556
BstV1I GCAGC 1 cut(s) 1741
BstV2I GAAGAC 2 cut(s) 549, 738
BstX2I RGATCY 3 cut(s) 429, 874, 1009
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 3 cut(s) 429, 874, 1009
Bsu15I ATCGAT 1 cut(s) 986
BsuRI GGCC 1 cut(s) 30
BsuTUI ATCGAT 1 cut(s) 986
BtgZI GCGATG 1 cut(s) 650
BtsCI GGATG 4 cut(s) 238, 457, 1132, 1876
BtsI GCAGTG 2 cut(s) 769, 1843
BtsIMutI CAGTG 5 cut(s) 769, 826, 1023, 1843, 1908
BtuMI TCGCGA 1 cut(s) 1556
Cac8I GCNNGC 1 cut(s) 760
Cfr13I GGNCC 3 cut(s) 967, 1688, 1793
Cfr9I CCCGGG 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 986
Csp6I GTAC 3 cut(s) 1134, 1364, 1596
CspCI CAANNNNNGTGG 2 cut(s) 210, 245
CviAII CATG 6 cut(s) 448, 713, 908, 1625, 1766, 1883
CviQI GTAC 3 cut(s) 1134, 1364, 1596
DraI TTTAAA 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 1024
EarI CTCTTC 1 cut(s) 1024
Eco130I CCWWGG 1 cut(s) 609
Eco47I GGWCC 3 cut(s) 967, 1688, 1793
Eco57I CTGAAG 3 cut(s) 870, 1200, 1290
Eco88I CYCGRG 1 cut(s) 90
EcoNI CCTNNNNNAGG 1 cut(s) 1590
EcoRI GAATTC 2 cut(s) 85, 1545
EcoRII CCWGG 1 cut(s) 1005
EcoT14I CCWWGG 1 cut(s) 609
EcoT22I ATGCAT 2 cut(s) 649, 1878
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 6 cut(s) 451, 716, 911, 1628, 1769, 1886
FaqI GGGAC 2 cut(s) 463, 1701
FatI CATG 6 cut(s) 447, 712, 907, 1624, 1765, 1882
FauI CCCGC 2 cut(s) 1859, 1950
FauNDI CATATG 1 cut(s) 1199
FbaI TGATCA 2 cut(s) 64, 1627
FblI GTMKAC 1 cut(s) 794
Fnu4HI GCNGC 1 cut(s) 1755
FokI GGATG 4 cut(s) 245, 464, 1139, 1863
Fsp4HI GCNGC 1 cut(s) 1755
FspBI CTAG 6 cut(s) 600, 750, 759, 872, 1356, 1530
GluI GCNGC 1 cut(s) 1755
GsaI CCCAGC 2 cut(s) 1466, 1523
HaeIII GGCC 1 cut(s) 30
HapII CCGG 2 cut(s) 91, 1924
Hin1II CATG 6 cut(s) 451, 716, 911, 1628, 1769, 1886
HincII GTYRAC 3 cut(s) 708, 795, 1292
HindII GTYRAC 3 cut(s) 708, 795, 1292
HpaII CCGG 2 cut(s) 91, 1924
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 7 cut(s) 708, 723, 795, 967, 1292, 1598, 1940
Hpy8I GTNNAC 7 cut(s) 708, 723, 795, 967, 1292, 1598, 1940
HpyAV CCTTC 6 cut(s) 148, 248, 426, 894, 1832, 1873
HpyCH4III ACNGT 6 cut(s) 784, 830, 929, 971, 1040, 1109
HpyCH4IV ACGT 5 cut(s) 576, 1294, 1456, 1513, 1729
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpySE526I ACGT 5 cut(s) 576, 1294, 1456, 1513, 1729
Hsp92II CATG 6 cut(s) 451, 716, 911, 1628, 1769, 1886
Ksp22I TGATCA 2 cut(s) 64, 1627
Lsp1109I GCAGC 1 cut(s) 1741
LweI GCATC 6 cut(s) 160, 175, 595, 634, 656, 1885
MaeI CTAG 6 cut(s) 600, 750, 759, 872, 1356, 1530
MaeII ACGT 5 cut(s) 576, 1294, 1456, 1513, 1729
MaeIII GTNAC 4 cut(s) 325, 359, 1249, 1658
MbiI CCGCTC 1 cut(s) 1854
MboII GAAGA 6 cut(s) 179, 439, 549, 557, 738, 1011
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 3 cut(s) 429, 874, 1009
MhlI GDGCHC 1 cut(s) 725
MlsI TGGCCA 1 cut(s) 30
MluNI TGGCCA 1 cut(s) 30
MlyI GAGTC 4 cut(s) 1478, 1535, 1552, 1678
MmeI TCCRAC 2 cut(s) 1083, 1931
Mox20I TGGCCA 1 cut(s) 30
Mph1103I ATGCAT 2 cut(s) 649, 1878
MscI TGGCCA 1 cut(s) 30
MseI TTAA 3 cut(s) 35, 54, 78
MslI CAYNNNNRTG 3 cut(s) 530, 912, 1881
Msp20I TGGCCA 1 cut(s) 30
MspI CCGG 2 cut(s) 91, 1924
MspR9I CCNGG 3 cut(s) 91, 92, 1007
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 1369
MvaI CCWGG 1 cut(s) 1007
MvnI CGCG 1 cut(s) 1556
MwoI GCNNNNNNNGC 1 cut(s) 36
NciI CCSGG 2 cut(s) 91, 92
NdeI CATATG 1 cut(s) 1199
NheI GCTAGC 1 cut(s) 758
NlaIII CATG 6 cut(s) 451, 716, 911, 1628, 1769, 1886
NlaIV GGNNCC 3 cut(s) 978, 1011, 1689
NmuCI GTSAC 1 cut(s) 1249
NruI TCGCGA 1 cut(s) 1556
NsiI ATGCAT 2 cut(s) 649, 1878
NspV TTCGAA 1 cut(s) 152
PctI GAATGC 1 cut(s) 1369
PkrI GCNGC 1 cut(s) 1756
PleI GAGTC 4 cut(s) 1477, 1534, 1552, 1677
PpsI GAGTC 4 cut(s) 1477, 1534, 1552, 1677
PshAI GACNNNNGTC 1 cut(s) 1404
PshBI ATTAAT 1 cut(s) 78
PsiI TTATAA 4 cut(s) 1565, 1572, 1676, 1781
Psp6I CCWGG 1 cut(s) 1005
PspFI CCCAGC 2 cut(s) 1462, 1519
PspGI CCWGG 1 cut(s) 1005
PspN4I GGNNCC 3 cut(s) 978, 1011, 1689
PspPI GGNCC 3 cut(s) 967, 1688, 1793
PstI CTGCAG 2 cut(s) 1543, 1759
PsuI RGATCY 3 cut(s) 429, 874, 1009
RruI TCGCGA 1 cut(s) 1556
RsaI GTAC 3 cut(s) 1135, 1365, 1597
RsaNI GTAC 3 cut(s) 1134, 1364, 1596
RseI CAYNNNNRTG 3 cut(s) 530, 912, 1881
SalI GTCGAC 1 cut(s) 793
SaqAI TTAA 3 cut(s) 35, 54, 78
SatI GCNGC 1 cut(s) 1755
Sau96I GGNCC 3 cut(s) 967, 1688, 1793
SchI GAGTC 4 cut(s) 1478, 1535, 1552, 1678
ScrFI CCNGG 3 cut(s) 91, 92, 1007
SduI GDGCHC 1 cut(s) 725
SfaNI GCATC 6 cut(s) 160, 175, 595, 634, 656, 1885
SfcI CTRYAG 5 cut(s) 829, 891, 1539, 1755, 1972
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 3 cut(s) 967, 1688, 1793
SmaI CCCGGG 1 cut(s) 92
SmiMI CAYNNNNRTG 3 cut(s) 530, 912, 1881
SmlI CTYRAG 2 cut(s) 8, 1771
SmoI CTYRAG 2 cut(s) 8, 1771
SsiI CCGC 4 cut(s) 506, 1852, 1880, 1957
SspMI CTAG 6 cut(s) 600, 750, 759, 872, 1356, 1530
StyD4I CCNGG 3 cut(s) 89, 90, 1005
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 6 cut(s) 784, 830, 929, 971, 1040, 1109
TaiI ACGT 5 cut(s) 579, 1297, 1459, 1516, 1732
TaqI TCGA 9 cut(s) 152, 794, 912, 986, 1385, 1610, 1667, 1745, 1825
TatI WGTACW 2 cut(s) 1133, 1363
Tru1I TTAA 3 cut(s) 35, 54, 78
Tru9I TTAA 3 cut(s) 35, 54, 78
TscAI CASTG 5 cut(s) 769, 833, 1030, 1843, 1908
TseFI GTSAC 1 cut(s) 1249
TseI GCWGC 1 cut(s) 1754
Tsp45I GTSAC 1 cut(s) 1249
TspDTI ATGAA 4 cut(s) 17, 267, 825, 1899
TspMI CCCGGG 1 cut(s) 90
TspRI CASTG 5 cut(s) 769, 833, 1030, 1843, 1908
VneI GTGCAC 1 cut(s) 721
VpaK11BI GGWCC 3 cut(s) 967, 1688, 1793
VspI ATTAAT 1 cut(s) 78
XagI CCTNNNNNAGG 1 cut(s) 1590
XapI RAATTY 2 cut(s) 85, 1545
XmaI CCCGGG 1 cut(s) 90
XmiI GTMKAC 1 cut(s) 794
XspI CTAG 6 cut(s) 600, 750, 759, 872, 1356, 1530
Zsp2I ATGCAT 2 cut(s) 649, 1878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.