Rh4BG364800

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000995
Physical Location & Seq
Reverse (-)
52235177 .. 52241783
6607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG364800.1

Sequence Viewer

Length: 1509 bp
ATGGACAAAAGTGTTACAGAATGCGACTTGCCAGAACTGACAGTTTGTTACAAAGAGAGTAGTTGCAATACTATCAAGGATATCTGCATAGATGAGGGAGTGCCTTCCCAAGAGAAGATCCGGTTTGAGACAGACGTGGATGAGAAGGCATTTTGCACTTATTTGTCTCCTGATAAGGATCAGAAACACCTACTGCTTGAACAACAAATGGACATTTTTATGACCATTCCAGATGATTTCAAGTCTTCTGCACAGAAATATGATGTAAAGAAGGATTTTGTCATTGCTTGTGATGCCAAGGATCTGGTGCAGAGAGAAGAAGCAATGTATGATGCATCAGAGAAAACTGCAATTGATGTGTCCAGAGACATGATTTCCCCTGAAAACTTGCGAACACAGAATTTGAACTCCAAGTCCTCTAGTAAGGCCAGCAATGAAGCTGTACAAGATACTGTTCAGATATCGAGTGACAAGGAAAGTGAAATAGCAGAAACTCACAGCAGTGCTTTGGTTTCAGCAAAGGAAGAATCTAAGGATATTGAGAATGGAGCGTTCTTGAAGCGAGATCCTGTTCCTTCAGCTGTAGAACTAAACTGCCATGTTGATAAGTTATCTGACATTAGTGAAGTGGAGAATGGAAGCATTACTGTTTGTTTGGACCGTTCAGCACCTATATCGATTAGTAGATTTCCAAACCTGGATCCTGTCCCTTCCACTGATGGATCAAACTGTCTTCTTGACGAGTTGTCCAACAATTTTAAAGCGGACAAAGGAGGAAGCAGTTCTTGTGGTTTGAACTGTTCAGACATTCTCGGGGATGTCTGTCCCCAAAATGGGTTCAGCAAACATATTGAAACTCAAAATATGTCTGAAGATGATAGTGATGACATATGTGATTCAAAAACTATTTCAAGCAAAGTTCAGCCTTGTTTAGCACCTGTGACTATTGCCAGAGGGGATTGTCCTGAAAATGGTGCCTGTCAACGTCCTGAAACTTCAAATACGTCCATGGTTGATGAGGACATTTCTGATTCAGAGATTGTTTCAAGCCAAGTTCAACATTCTTTAGTCCCTGCGACTACTGCCAGAGATGAATATCCTAAAGAGGGGAATTCTAATGCACAGGATGTTCCGATCCAAGTTCAACATGAGGTGGGAGAGACTAGTTTCTCCGCAGGAGTTCCTTTCTCGACCCTTATAAATTATAAGGAACCAATACCTTATTCAGGTACACACAATGCTTCGAGTGAAGTGCAACATGATCAGGGGGAATCAAGTTTCTCTGCAGTACGTAACTCATTGAGTCTTATAAATAGTTCGGGACCAATACCTTATTCTGATTCAGATGGATTCCTTCTTCAACGTGATCTGGGAGATTCGAGTTTTGCTGCAGTAGGTCATGTCTCGAGTCTCATAAATAGTTCGGGACCAATACCTTATTCTGGCAGTATCTCCCTTCGATCAGACAGCAGCGCAACCAGCACCCGCTCATTTGCCTTCCCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

502

Amino Acids

54.31

Weight (kDa)

4.39

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1199, 1206, 1310
AccB1I GGYRCC 1 cut(s) 974
AccBSI CCGCTC 1 cut(s) 1488
AciI CCGC 3 cut(s) 764, 1173, 1486
AclWI GGATC 8 cut(s) 112, 186, 309, 560, 695, 708, 730, 1129
AcsI RAATTY 2 cut(s) 400, 1111
AcuI CTGAAG 2 cut(s) 561, 891
AfaI GTAC 3 cut(s) 444, 1231, 1290
AfiI CCNNNNNNNGG 6 cut(s) 833, 834, 971, 1106, 1226, 1442
AhdI GACNNNNNGTC 1 cut(s) 745
AhlI ACTAGT 1 cut(s) 1163
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 1 cut(s) 696
AjuI GAANNNNNNNTTGG 6 cut(s) 1207, 1239, 1318, 1350, 1423, 1455
AleI CACNNNNGTG 1 cut(s) 501
AloI GAACNNNNNNTCC 2 cut(s) 398, 430
AluBI AGCT 2 cut(s) 440, 581
AluI AGCT 2 cut(s) 440, 581
Alw26I GTCTC 6 cut(s) 122, 171, 360, 1154, 1408, 1415
AlwI GGATC 8 cut(s) 112, 186, 309, 560, 695, 708, 730, 1129
Ama87I CYCGRG 2 cut(s) 812, 1405
AoxI GGCC 1 cut(s) 426
ApeKI GCWGC 2 cut(s) 1388, 1470
ApoI RAATTY 2 cut(s) 400, 1111
Asp700I GAANNNNTTC 1 cut(s) 781
AspLEI GCGC 1 cut(s) 1475
AspS9I GGNCC 3 cut(s) 658, 1322, 1427
AvaI CYCGRG 2 cut(s) 812, 1405
AvaII GGWCC 3 cut(s) 658, 1322, 1427
BamHI GGATCC 1 cut(s) 700
BanI GGYRCC 1 cut(s) 974
BbsI GAAGAC 2 cut(s) 237, 725
BbvI GCAGC 2 cut(s) 1375, 1482
BccI CCATC 2 cut(s) 713, 1340
BcgI CGANNNNNNTGC 4 cut(s) 657, 691, 1234, 1268
BciT130I CCWGG 1 cut(s) 698
BclI TGATCA 1 cut(s) 1261
BcoDI GTCTC 6 cut(s) 122, 171, 360, 1154, 1408, 1415
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 2 cut(s) 420, 1164
BfmI CTRYAG 3 cut(s) 582, 1284, 1389
BisI GCNGC 2 cut(s) 1389, 1471
BlsI GCNGC 2 cut(s) 1390, 1472
Bme1390I CCNGG 1 cut(s) 698
Bme18I GGWCC 3 cut(s) 658, 1322, 1427
BmeRI GACNNNNNGTC 1 cut(s) 745
BmeT110I CYCGRG 2 cut(s) 812, 1405
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 658, 1322, 1427
BmiI GGNNCC 5 cut(s) 702, 976, 1212, 1323, 1428
BmrFI CCNGG 1 cut(s) 698
BmsI GCATC 3 cut(s) 283, 322, 344
BpiI GAAGAC 2 cut(s) 237, 725
Bsa29I ATCGAT 1 cut(s) 677
BsaAI YACGTR 1 cut(s) 1292
BsaBI GATNNNNATC 2 cut(s) 177, 1095
BsaJI CCNNGG 2 cut(s) 297, 1008
BsaWI WCCGGW 1 cut(s) 120
Bsc4I CCNNNNNNNGG 6 cut(s) 833, 834, 971, 1106, 1226, 1442
Bse3DI GCAATG 3 cut(s) 282, 330, 439
Bse8I GATNNNNATC 2 cut(s) 177, 1095
BseBI CCWGG 1 cut(s) 698
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 2 cut(s) 297, 1008
BseGI GGATG 3 cut(s) 145, 823, 1132
BseJI GATNNNNATC 2 cut(s) 177, 1095
BseLI CCNNNNNNNGG 6 cut(s) 833, 834, 971, 1106, 1226, 1442
BseMI GCAATG 3 cut(s) 282, 330, 439
BseXI GCAGC 2 cut(s) 1375, 1482
BsgI GTGCAG 2 cut(s) 234, 329
BshFI GGCC 1 cut(s) 428
BshNI GGYRCC 1 cut(s) 974
BshVI ATCGAT 1 cut(s) 677
BsiHKCI CYCGRG 2 cut(s) 812, 1405
BsiSI CCGG 1 cut(s) 121
BslFI GGGAC 5 cut(s) 692, 810, 1055, 1335, 1440
BslI CCNNNNNNNGG 6 cut(s) 833, 834, 971, 1106, 1226, 1442
BsmAI GTCTC 6 cut(s) 122, 171, 360, 1154, 1408, 1415
BsmFI GGGAC 5 cut(s) 692, 810, 1055, 1335, 1440
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 1 cut(s) 428
BsoBI CYCGRG 2 cut(s) 812, 1405
Bsp1407I TGTACA 1 cut(s) 442
Bsp19I CCATGG 1 cut(s) 1008
BspACI CCGC 3 cut(s) 764, 1173, 1486
BspANI GGCC 1 cut(s) 428
BspDI ATCGAT 1 cut(s) 677
BspLI GGNNCC 5 cut(s) 702, 976, 1212, 1323, 1428
BspMAI CTGCAG 2 cut(s) 1288, 1393
BspPI GGATC 8 cut(s) 112, 186, 309, 560, 695, 708, 730, 1129
BspT107I GGYRCC 1 cut(s) 974
BsrBI CCGCTC 1 cut(s) 1488
BsrDI GCAATG 3 cut(s) 282, 330, 439
BsrGI TGTACA 1 cut(s) 442
BssECI CCNNGG 2 cut(s) 297, 1008
BssT1I CCWWGG 2 cut(s) 297, 1008
Bst2UI CCWGG 1 cut(s) 698
Bst4CI ACNGT 6 cut(s) 43, 454, 649, 662, 731, 800
BstAUI TGTACA 1 cut(s) 442
BstBAI YACGTR 1 cut(s) 1292
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 1 cut(s) 531
BstDSI CCRYGG 1 cut(s) 1008
BstENI CCTNNNNNAGG 1 cut(s) 1224
BstF5I GGATG 3 cut(s) 145, 823, 1132
BstHHI GCGC 1 cut(s) 1475
BstMAI GTCTC 6 cut(s) 122, 171, 360, 1154, 1408, 1415
BstMWI GCNNNNNNNGC 3 cut(s) 293, 1082, 1479
BstNI CCWGG 1 cut(s) 698
BstSCI CCNGG 1 cut(s) 696
BstSFI CTRYAG 3 cut(s) 582, 1284, 1389
BstSNI TACGTA 1 cut(s) 1292
BstV1I GCAGC 2 cut(s) 1375, 1482
BstV2I GAAGAC 2 cut(s) 237, 725
BstX2I RGATCY 4 cut(s) 117, 301, 565, 700
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 4 cut(s) 117, 301, 565, 700
Bsu15I ATCGAT 1 cut(s) 677
BsuRI GGCC 1 cut(s) 428
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 1008
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 3 cut(s) 145, 823, 1132
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 508, 714
Cac8I GCNNGC 1 cut(s) 430
CfoI GCGC 1 cut(s) 1475
Cfr13I GGNCC 3 cut(s) 658, 1322, 1427
ClaI ATCGAT 1 cut(s) 677
Csp6I GTAC 3 cut(s) 443, 1230, 1289
CviAII CATG 6 cut(s) 370, 599, 1009, 1148, 1259, 1400
CviJI RGCY 5 cut(s) 428, 440, 581, 925, 1050
CviKI_1 RGCY 5 cut(s) 428, 440, 581, 925, 1050
CviQI GTAC 3 cut(s) 443, 1230, 1289
DdeI CTNAG 1 cut(s) 531
DraI TTTAAA 1 cut(s) 760
DriI GACNNNNNGTC 1 cut(s) 745
Eam1105I GACNNNNNGTC 1 cut(s) 745
Eco105I TACGTA 1 cut(s) 1292
Eco130I CCWWGG 2 cut(s) 297, 1008
Eco32I GATATC 2 cut(s) 82, 462
Eco47I GGWCC 3 cut(s) 658, 1322, 1427
Eco57I CTGAAG 2 cut(s) 561, 891
Eco88I CYCGRG 2 cut(s) 812, 1405
EcoNI CCTNNNNNAGG 1 cut(s) 1224
EcoRI GAATTC 1 cut(s) 1111
EcoRII CCWGG 1 cut(s) 696
EcoRV GATATC 2 cut(s) 82, 462
EcoT14I CCWWGG 2 cut(s) 297, 1008
EcoT22I ATGCAT 1 cut(s) 337
ErhI CCWWGG 2 cut(s) 297, 1008
FaeI CATG 6 cut(s) 373, 602, 1012, 1151, 1262, 1403
FalI AAGNNNNNCTT 2 cut(s) 769, 801
FaqI GGGAC 5 cut(s) 692, 810, 1055, 1335, 1440
FatI CATG 6 cut(s) 369, 598, 1008, 1147, 1258, 1399
FauI CCCGC 1 cut(s) 1493
FauNDI CATATG 1 cut(s) 890
FbaI TGATCA 1 cut(s) 1261
Fnu4HI GCNGC 2 cut(s) 1389, 1471
FokI GGATG 3 cut(s) 152, 830, 1139
Fsp4HI GCNGC 2 cut(s) 1389, 1471
FspBI CTAG 2 cut(s) 420, 1164
GlaI GCGC 1 cut(s) 1474
GluI GCNGC 2 cut(s) 1389, 1471
HaeIII GGCC 1 cut(s) 428
HapII CCGG 1 cut(s) 121
HhaI GCGC 1 cut(s) 1475
Hin1II CATG 6 cut(s) 373, 602, 1012, 1151, 1262, 1403
Hin6I GCGC 1 cut(s) 1473
HinP1I GCGC 1 cut(s) 1473
HincII GTYRAC 1 cut(s) 983
HindII GTYRAC 1 cut(s) 983
HinfI GANTC 9 cut(s) 527, 896, 1031, 1271, 1303, 1340, 1350, 1376, 1408
HpaII CCGG 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 983, 1232
Hpy8I GTNNAC 2 cut(s) 983, 1232
HpyAV CCTTC 8 cut(s) 114, 139, 265, 585, 720, 1364, 1466, 1507
HpyCH4III ACNGT 6 cut(s) 43, 454, 649, 662, 731, 800
HpyCH4IV ACGT 5 cut(s) 135, 985, 1004, 1291, 1363
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 1082, 1479
HpyF3I CTNAG 1 cut(s) 531
HpySE526I ACGT 5 cut(s) 135, 985, 1004, 1291, 1363
Hsp92II CATG 6 cut(s) 373, 602, 1012, 1151, 1262, 1403
HspAI GCGC 1 cut(s) 1473
Ksp22I TGATCA 1 cut(s) 1261
LmnI GCTCC 1 cut(s) 548
Lsp1109I GCAGC 2 cut(s) 1375, 1482
LweI GCATC 3 cut(s) 283, 322, 344
MaeI CTAG 2 cut(s) 420, 1164
MaeII ACGT 5 cut(s) 135, 985, 1004, 1291, 1363
MaeIII GTNAC 5 cut(s) 13, 47, 467, 940, 1292
MbiI CCGCTC 1 cut(s) 1488
MboII GAAGA 7 cut(s) 127, 237, 329, 536, 725, 884, 1349
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 4 cut(s) 117, 301, 565, 700
MluCI AATT 5 cut(s) 351, 400, 754, 1111, 1201
MlyI GAGTC 2 cut(s) 1312, 1417
MmeI TCCRAC 1 cut(s) 774
MnlI CCTC 7 cut(s) 88, 427, 767, 947, 1012, 1099, 1144
Mph1103I ATGCAT 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 781
MseI TTAA 1 cut(s) 759
MslI CAYNNNNRTG 2 cut(s) 218, 501
MspA1I CMGCKG 1 cut(s) 581
MspI CCGG 1 cut(s) 121
MspR9I CCNGG 1 cut(s) 698
MunI CAATTG 1 cut(s) 351
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 1 cut(s) 698
MwoI GCNNNNNNNGC 3 cut(s) 293, 1082, 1479
NcoI CCATGG 1 cut(s) 1008
NdeI CATATG 1 cut(s) 890
NlaIII CATG 6 cut(s) 373, 602, 1012, 1151, 1262, 1403
NlaIV GGNNCC 5 cut(s) 702, 976, 1212, 1323, 1428
NmuCI GTSAC 2 cut(s) 467, 940
NsiI ATGCAT 1 cut(s) 337
OliI CACNNNNGTG 1 cut(s) 501
PaeR7I CTCGAG 1 cut(s) 1405
PctI GAATGC 1 cut(s) 26
PdmI GAANNNNTTC 1 cut(s) 781
PfeI GAWTC 7 cut(s) 527, 896, 1031, 1271, 1340, 1350, 1376
PkrI GCNGC 2 cut(s) 1390, 1472
PleI GAGTC 2 cut(s) 1311, 1416
PpsI GAGTC 2 cut(s) 1311, 1416
Ppu21I YACGTR 1 cut(s) 1292
PsiI TTATAA 3 cut(s) 1199, 1206, 1310
Psp6I CCWGG 1 cut(s) 696
PspGI CCWGG 1 cut(s) 696
PspN4I GGNNCC 5 cut(s) 702, 976, 1212, 1323, 1428
PspPI GGNCC 3 cut(s) 658, 1322, 1427
PstI CTGCAG 2 cut(s) 1288, 1393
PsuI RGATCY 4 cut(s) 117, 301, 565, 700
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 3 cut(s) 444, 1231, 1290
RsaNI GTAC 3 cut(s) 443, 1230, 1289
RseI CAYNNNNRTG 2 cut(s) 218, 501
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 2 cut(s) 1389, 1471
Sau96I GGNCC 3 cut(s) 658, 1322, 1427
SchI GAGTC 2 cut(s) 1312, 1417
ScrFI CCNGG 1 cut(s) 698
SfaNI GCATC 3 cut(s) 283, 322, 344
SfcI CTRYAG 3 cut(s) 582, 1284, 1389
Sfr274I CTCGAG 1 cut(s) 1405
SinI GGWCC 3 cut(s) 658, 1322, 1427
SlaI CTCGAG 1 cut(s) 1405
SmiMI CAYNNNNRTG 2 cut(s) 218, 501
SmlI CTYRAG 1 cut(s) 1405
SmoI CTYRAG 1 cut(s) 1405
SnaBI TACGTA 1 cut(s) 1292
SpeI ACTAGT 1 cut(s) 1163
Sse9I AATT 5 cut(s) 351, 400, 754, 1111, 1201
SsiI CCGC 3 cut(s) 764, 1173, 1486
SspMI CTAG 2 cut(s) 420, 1164
StyD4I CCNGG 1 cut(s) 696
StyI CCWWGG 2 cut(s) 297, 1008
TaaI ACNGT 6 cut(s) 43, 454, 649, 662, 731, 800
TaiI ACGT 5 cut(s) 138, 988, 1007, 1294, 1366
TaqI TCGA 7 cut(s) 464, 677, 1190, 1244, 1379, 1406, 1459
TasI AATT 5 cut(s) 351, 400, 754, 1111, 1201
TatI WGTACW 1 cut(s) 442
TfiI GAWTC 7 cut(s) 527, 896, 1031, 1271, 1340, 1350, 1376
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 2 cut(s) 508, 721
TseFI GTSAC 2 cut(s) 467, 940
TseI GCWGC 2 cut(s) 1388, 1470
Tsp45I GTSAC 2 cut(s) 467, 940
TspDTI ATGAA 2 cut(s) 450, 1107
TspRI CASTG 2 cut(s) 508, 721
VpaK11BI GGWCC 3 cut(s) 658, 1322, 1427
XagI CCTNNNNNAGG 1 cut(s) 1224
XapI RAATTY 2 cut(s) 400, 1111
XhoI CTCGAG 1 cut(s) 1405
XmnI GAANNNNTTC 1 cut(s) 781
XspI CTAG 2 cut(s) 420, 1164
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.