MD14G1216700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000995
Physical Location & Seq
Forward (+)
30000344 .. 30002694
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1216700.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGATGGCCATTCAACCGCGGTCTTCCTGCCTGTTTACAAGTCCTTCAAGTTCAAAAGTTCCGAAGCCACGAAGGGATTGAAGGTGGAGAAAAGTACTAGTTCGAACGATGATAATCTGTTCATTTCCGATTTGCTTAGTCAAGACAGTGAGGCGGTTCTAAAACCACATCCAGATGACAGTCAGAATATGCACGCTACAAGGGACGCCGTGACAGCAAATGATTGCGCGGCTTGGACGAATTCTACTAGAACGGCTGCTGTGGACTATGACTTGCCAGAGTTGGTTGCATTCCTTCAAGGGAGCAGCAACTACCAGTTCTTTAAGGACATTGGCATTGACAAGGAAGTTCGATGCGAGGACAAGTGTTTCGTACAGCACTGTGAATCCTATCATAGTAGCTTTTCTTGCATACTTGATCCTGATCTGTACAGCGAGGGCGAGTCAATTAGAGAATCTCTGGACTCGGAGTCAACCACTTCAACCGATGAGAATGACGGCAAAAAGGATGATCTTTCAAATGATCATTCAATCAAGAAGATCATATCCAAAAAAGTGATTTTGGTTAGACAGGGTACAAGTAACGCGGCCTTGGCCGCAAACTCAATGGGACGATCTATAACTAAGGCAATTGGCGACAACAGCCGGACGAGCAATGCCTCTTTAAAAGGTGACCTAGAAAATACAAGCGGAGCAAAGTTTCTTGCCCCAGAAACGGAAAACTTGTTGAGGCATGATTCGTTGAAAGATAGGATGTCAGATAGCCTAGCAGAATCGTGTCAACGACACCTGCATTGCCGCGGAGAATCCAGTTCCTCAGCATCCGAACTTCCTTCGGGCTCATTTTCGTACTTGGGAGGAATACCGGGTTTTGGCAGCATCTCTCATCGGTCGAGCAGCAGCACAACTAGCTCCGAGTCTTTCGCTTTTCCCATATTATCCTCGGAATGGAATGGCAGCCCGGCGAGAATGGGGAGCGCTGACGAGCGACAATTGCACCAGCACCGGCACTGGGTGATGCGATTTCTCTGCTGTAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

348

Amino Acids

38.06

Weight (kDa)

5.54

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 798
Acc36I ACCTGC 1 cut(s) 798
AccII CGCG 4 cut(s) 20, 230, 587, 801
AciI CCGC 9 cut(s) 18, 20, 155, 230, 587, 597, 690, 799, 801
AclWI GGATC 1 cut(s) 413
AcoI YGGCCR 2 cut(s) 7, 594
AcsI RAATTY 2 cut(s) 241, 1037
AcyI GRCGYC 1 cut(s) 207
AdeI CACNNNGTG 1 cut(s) 1015
AfaI GTAC 5 cut(s) 97, 375, 431, 577, 851
AfeI AGCGCT 1 cut(s) 979
AfiI CCNNNNNNNGG 4 cut(s) 715, 872, 948, 1012
AgsI TTSAA 9 cut(s) 15, 49, 55, 82, 299, 483, 519, 531, 745
AhdI GACNNNNNGTC 1 cut(s) 469
AhlI ACTAGT 1 cut(s) 98
AluBI AGCT 2 cut(s) 402, 912
AluI AGCT 2 cut(s) 402, 912
AlwI GGATC 1 cut(s) 413
Aor51HI AGCGCT 1 cut(s) 979
AoxI GGCC 3 cut(s) 7, 588, 594
ApeKI GCWGC 6 cut(s) 257, 306, 876, 897, 900, 957
ApoI RAATTY 2 cut(s) 241, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AspLEI GCGC 2 cut(s) 230, 980
AsuC2I CCSGG 2 cut(s) 867, 962
AsuHPI GGTGA 2 cut(s) 683, 1027
AsuII TTCGAA 1 cut(s) 104
BalI TGGCCA 1 cut(s) 9
BanII GRGCYC 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 16
BbvCI CCTCAGC 1 cut(s) 817
BbvI GCAGC 6 cut(s) 244, 318, 888, 909, 912, 969
BceAI ACGGC 3 cut(s) 194, 270, 514
BcgI CGANNNNNNTGC 1 cut(s) 1011
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 2 cut(s) 867, 962
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 5 cut(s) 99, 249, 677, 767, 909
BfoI RGCGCY 1 cut(s) 981
BfuAI ACCTGC 1 cut(s) 798
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 2 cut(s) 867, 962
BmeRI GACNNNNNGTC 1 cut(s) 469
BmrFI CCNGG 2 cut(s) 867, 962
BmrI ACTGGG 1 cut(s) 1021
BmsI GCATC 4 cut(s) 344, 830, 888, 1008
BmuI ACTGGG 1 cut(s) 1021
BpiI GAAGAC 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 817
Bpu14I TTCGAA 1 cut(s) 104
BpuMI CCSGG 2 cut(s) 867, 962
BsaBI GATNNNNATC 2 cut(s) 114, 423
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 4 cut(s) 18, 591, 799, 942
Bsc4I CCNNNNNNNGG 4 cut(s) 715, 872, 948, 1012
Bse118I RCCGGY 1 cut(s) 1005
Bse1I ACTGG 3 cut(s) 316, 810, 1016
Bse3DI GCAATG 2 cut(s) 661, 793
Bse8I GATNNNNATC 2 cut(s) 114, 423
BseDI CCNNGG 4 cut(s) 18, 591, 799, 942
BseGI GGATG 5 cut(s) 10, 169, 514, 759, 821
BseJI GATNNNNATC 2 cut(s) 114, 423
BseLI CCNNNNNNNGG 4 cut(s) 715, 872, 948, 1012
BseMI GCAATG 2 cut(s) 661, 793
BseMII CTCAG 1 cut(s) 831
BseNI ACTGG 3 cut(s) 316, 810, 1016
BseXI GCAGC 6 cut(s) 244, 318, 888, 909, 912, 969
Bsh1236I CGCG 4 cut(s) 20, 230, 587, 801
Bsh1285I CGRYCG 1 cut(s) 893
BshFI GGCC 3 cut(s) 9, 590, 596
BsiEI CGRYCG 1 cut(s) 893
BsiSI CCGG 4 cut(s) 646, 866, 962, 1006
BslFI GGGAC 2 cut(s) 218, 624
BslI CCNNNNNNNGG 4 cut(s) 715, 872, 948, 1012
BsmFI GGGAC 2 cut(s) 218, 624
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 3 cut(s) 9, 590, 596
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 842
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 6 cut(s) 418, 424, 511, 523, 540, 614
BspACI CCGC 9 cut(s) 18, 20, 155, 230, 587, 597, 690, 799, 801
BspANI GGCC 3 cut(s) 9, 590, 596
BspCNI CTCAG 1 cut(s) 830
BspFNI CGCG 4 cut(s) 20, 230, 587, 801
BspMI ACCTGC 1 cut(s) 798
BspPI GGATC 1 cut(s) 413
BspT104I TTCGAA 1 cut(s) 104
BsrDI GCAATG 2 cut(s) 661, 793
BsrFI RCCGGY 1 cut(s) 1005
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 3 cut(s) 316, 810, 1016
BssAI RCCGGY 1 cut(s) 1005
BssECI CCNNGG 4 cut(s) 18, 591, 799, 942
BssMI GATC 6 cut(s) 418, 424, 511, 523, 540, 614
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 591
Bst4CI ACNGT 3 cut(s) 149, 182, 383
BstACI GRCGYC 1 cut(s) 207
BstAUI TGTACA 1 cut(s) 429
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 3 cut(s) 137, 624, 817
BstDSI CCRYGG 2 cut(s) 18, 799
BstEII GGTNACC 1 cut(s) 671
BstF5I GGATG 5 cut(s) 10, 169, 514, 759, 821
BstFNI CGCG 4 cut(s) 20, 230, 587, 801
BstH2I RGCGCY 1 cut(s) 981
BstHHI GCGC 2 cut(s) 230, 980
BstKTI GATC 6 cut(s) 421, 427, 514, 526, 543, 617
BstMBI GATC 6 cut(s) 418, 424, 511, 523, 540, 614
BstMCI CGRYCG 1 cut(s) 893
BstMWI GCNNNNNNNGC 8 cut(s) 215, 408, 593, 596, 642, 651, 909, 994
BstPI GGTNACC 1 cut(s) 671
BstSCI CCNGG 2 cut(s) 865, 960
BstUI CGCG 4 cut(s) 20, 230, 587, 801
BstV1I GCAGC 6 cut(s) 244, 318, 888, 909, 912, 969
BstV2I GAAGAC 1 cut(s) 16
BsuRI GGCC 3 cut(s) 9, 590, 596
BtgI CCRYGG 2 cut(s) 18, 799
BtsCI GGATG 5 cut(s) 10, 169, 514, 759, 821
BtsIMutI CAGTG 3 cut(s) 154, 379, 1009
BveI ACCTGC 1 cut(s) 798
Cac8I GCNNGC 1 cut(s) 195
CfoI GCGC 2 cut(s) 230, 980
Cfr10I RCCGGY 1 cut(s) 1005
Cfr42I CCGCGG 2 cut(s) 21, 802
CseI GACGC 1 cut(s) 215
Csp6I GTAC 5 cut(s) 96, 374, 430, 576, 850
CviAII CATG 1 cut(s) 734
CviQI GTAC 5 cut(s) 96, 374, 430, 576, 850
DdeI CTNAG 3 cut(s) 137, 624, 817
DpnI GATC 6 cut(s) 420, 426, 513, 525, 542, 616
DpnII GATC 6 cut(s) 418, 424, 511, 523, 540, 614
DraI TTTAAA 1 cut(s) 666
DraIII CACNNNGTG 1 cut(s) 1015
DriI GACNNNNNGTC 1 cut(s) 469
EaeI YGGCCR 2 cut(s) 7, 594
Eam1105I GACNNNNNGTC 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 842
Eco47III AGCGCT 1 cut(s) 979
Eco91I GGTNACC 1 cut(s) 671
EcoO65I GGTNACC 1 cut(s) 671
EcoRI GAATTC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 591
EcoT38I GRGCYC 1 cut(s) 842
ErhI CCWWGG 1 cut(s) 591
FaeI CATG 1 cut(s) 737
FaiI YATR 8 cut(s) 191, 270, 396, 413, 545, 620, 735, 935
FaqI GGGAC 2 cut(s) 218, 624
FatI CATG 1 cut(s) 733
FbaI TGATCA 1 cut(s) 523
FokI GGATG 5 cut(s) 17, 156, 521, 766, 808
FriOI GRGCYC 1 cut(s) 842
FspBI CTAG 5 cut(s) 99, 249, 677, 767, 909
GlaI GCGC 2 cut(s) 229, 979
HaeII RGCGCY 1 cut(s) 981
HaeIII GGCC 3 cut(s) 9, 590, 596
HapII CCGG 4 cut(s) 646, 866, 962, 1006
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 230, 980
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 1 cut(s) 737
Hin6I GCGC 2 cut(s) 228, 978
HinP1I GCGC 2 cut(s) 228, 978
HincII GTYRAC 2 cut(s) 474, 782
HindII GTYRAC 2 cut(s) 474, 782
HinfI GANTC 9 cut(s) 386, 443, 455, 464, 470, 737, 773, 806, 917
HpaII CCGG 4 cut(s) 646, 866, 962, 1006
HphI GGTGA 2 cut(s) 683, 1027
Hpy166II GTNNAC 4 cut(s) 37, 265, 474, 782
Hpy188I TCNGA 9 cut(s) 64, 130, 186, 469, 760, 826, 916, 946, 1043
Hpy188III TCNNGA 5 cut(s) 143, 173, 422, 461, 535
Hpy8I GTNNAC 4 cut(s) 37, 265, 474, 782
HpyAV CCTTC 5 cut(s) 55, 67, 76, 305, 843
HpyCH4III ACNGT 3 cut(s) 149, 182, 383
HpyCH4V TGCA 5 cut(s) 193, 290, 411, 793, 997
HpyF10VI GCNNNNNNNGC 8 cut(s) 215, 408, 593, 596, 642, 651, 909, 994
HpyF3I CTNAG 3 cut(s) 137, 624, 817
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 1 cut(s) 737
HspAI GCGC 2 cut(s) 228, 978
Ksp22I TGATCA 1 cut(s) 523
KspI CCGCGG 2 cut(s) 21, 802
Kzo9I GATC 6 cut(s) 418, 424, 511, 523, 540, 614
LmnI GCTCC 4 cut(s) 303, 692, 917, 975
Lsp1109I GCAGC 6 cut(s) 244, 318, 888, 909, 912, 969
LweI GCATC 4 cut(s) 344, 830, 888, 1008
MaeI CTAG 5 cut(s) 99, 249, 677, 767, 909
MaeIII GTNAC 3 cut(s) 211, 581, 671
MalI GATC 6 cut(s) 420, 426, 513, 525, 542, 616
MboI GATC 6 cut(s) 418, 424, 511, 523, 540, 614
MboII GAAGA 2 cut(s) 16, 550
MfeI CAATTG 2 cut(s) 630, 992
MhlI GDGCHC 1 cut(s) 842
MlsI TGGCCA 1 cut(s) 9
MluCI AATT 5 cut(s) 241, 447, 630, 992, 1037
MluNI TGGCCA 1 cut(s) 9
MlyI GAGTC 4 cut(s) 452, 458, 479, 926
MnlI CCTC 8 cut(s) 145, 352, 430, 670, 723, 826, 851, 952
Mox20I TGGCCA 1 cut(s) 9
MscI TGGCCA 1 cut(s) 9
MseI TTAA 2 cut(s) 324, 665
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 9
MspA1I CMGCKG 2 cut(s) 20, 801
MspI CCGG 4 cut(s) 646, 866, 962, 1006
MspR9I CCNGG 2 cut(s) 867, 962
MunI CAATTG 2 cut(s) 630, 992
Mva1269I GAATGC 1 cut(s) 290
MvnI CGCG 4 cut(s) 20, 230, 587, 801
MwoI GCNNNNNNNGC 8 cut(s) 215, 408, 593, 596, 642, 651, 909, 994
NciI CCSGG 2 cut(s) 867, 962
NdeII GATC 6 cut(s) 418, 424, 511, 523, 540, 614
NlaIII CATG 1 cut(s) 737
NmuCI GTSAC 2 cut(s) 211, 671
NspV TTCGAA 1 cut(s) 104
PaqCI CACCTGC 1 cut(s) 798
PctI GAATGC 1 cut(s) 290
PfeI GAWTC 5 cut(s) 386, 455, 737, 773, 806
PleI GAGTC 4 cut(s) 451, 458, 478, 925
PpsI GAGTC 4 cut(s) 451, 458, 478, 925
PspEI GGTNACC 1 cut(s) 671
RsaI GTAC 5 cut(s) 97, 375, 431, 577, 851
RsaNI GTAC 5 cut(s) 96, 374, 430, 576, 850
RseI CAYNNNNRTG 1 cut(s) 174
SacII CCGCGG 2 cut(s) 21, 802
SaqAI TTAA 2 cut(s) 324, 665
Sau3AI GATC 6 cut(s) 418, 424, 511, 523, 540, 614
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 4 cut(s) 452, 458, 479, 926
ScrFI CCNGG 2 cut(s) 867, 962
SduI GDGCHC 1 cut(s) 842
SetI ASST 6 cut(s) 87, 404, 673, 678, 792, 914
SfaNI GCATC 4 cut(s) 344, 830, 888, 1008
Sfr303I CCGCGG 2 cut(s) 21, 802
SfuI TTCGAA 1 cut(s) 104
SgrBI CCGCGG 2 cut(s) 21, 802
SmiMI CAYNNNNRTG 1 cut(s) 174
SpeI ACTAGT 1 cut(s) 98
Sse9I AATT 5 cut(s) 241, 447, 630, 992, 1037
SsiI CCGC 9 cut(s) 18, 20, 155, 230, 587, 597, 690, 799, 801
SspMI CTAG 5 cut(s) 99, 249, 677, 767, 909
StyD4I CCNGG 2 cut(s) 865, 960
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 3 cut(s) 149, 182, 383
TaqI TCGA 3 cut(s) 104, 352, 893
TaqII GACCGA 1 cut(s) 879
TasI AATT 5 cut(s) 241, 447, 630, 992, 1037
TatI WGTACW 2 cut(s) 95, 429
TauI GCSGC 4 cut(s) 233, 590, 599, 801
TfiI GAWTC 5 cut(s) 386, 455, 737, 773, 806
Tru1I TTAA 2 cut(s) 324, 665
Tru9I TTAA 2 cut(s) 324, 665
TscAI CASTG 3 cut(s) 154, 386, 1016
TseFI GTSAC 2 cut(s) 211, 671
TseI GCWGC 6 cut(s) 257, 306, 876, 897, 900, 957
Tsp45I GTSAC 2 cut(s) 211, 671
TspDTI ATGAA 1 cut(s) 112
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 3 cut(s) 154, 386, 1016
XapI RAATTY 2 cut(s) 241, 1037
XspI CTAG 5 cut(s) 99, 249, 677, 767, 909
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.