Rorug04G0300000

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000995
Physical Location & Seq
Forward (+)
47474890 .. 47477958
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0300000.1

Sequence Viewer

Length: 1272 bp
ATGGCGCGGAAGAAGATCAGAGAGTACGATTCGAAGAGACTGCTGAAGCAGCACTTGAAGCGAGTAGCCGGAATTGACCTCCAGATCAACTCGGCTCAGGTGACGGAGGCGACCGACTTCACCGAATTGGCCAACAACGAGACATGGCTTTCGTCGACCAGACTCGTCGTCAAGCCGGACATGCTTTTCGGGAAGCGCGGGAAGAGTGGCCTCGTCGCGCTCAATTTGGACATGGCTCAGGTCGCCGACTTCGTCAAGCAGCGTCTCGGTAAGGAGGTTGAGATGGGAGGGTGTAAGGCGCCTATAACGACGTTTATAGTGGAGCCGTTTATGCCTCATGATCAAGAGTTTTACCTCTCGATTGTATCCGAAAGGCTCGGGAGCACGATAAGCTTTTCGGAGTGTGGAGGTATTGAGATTGAAGAGAATTGGGACAAGGTCAAGACTATTTTTCTTCCATCCGAGAAGTCTCTCACGAATGAGGCTTGTGCTCCGCTGATTGCTACTCTTCCACTGGAGGTCAAGGAGAAAATTGGAGATTTCATAAAAGCCGTATTTGCTGTATTTCAGGATTTGGATTTTAGCTTCCTGGAAATGAACCCGTTTACCTTGGTTAATGGTGAACCTTTTCCATTAGATATGAGGGGGGAGCTAGATGACACTGCGGCTTTCAAGAACTTCAACAAATGGGGTAATGTGGAGTTTCCACTGCCTTTTGGCAGAGTTTTAAGTCCAACAGAAACATTTATTCATACTTTGGATGAAAAGACAAGTGCGTCCTTAAAATTCACAGTGCTGAATCCAAAAGGACGCATCTGGACCATGGTAGCTGGAGGTGGTGCAAGCGTTATTTATGCTGATACTGTTGGAGATTTGGGTTATGCATCAGAACTTGGAAACTATGCAGAGTATAGTGGAGCTCCAAATGAAGAAGAGGTGTTGCAATATGCAAGAGTAGTGATTGATTGTGCAACAGCAAATCCAGATGGTCGTAAAAGAGCTCTTCTGATTGGAGGTGGTATAGCTAATTTCACAGATGTTGCTGCTACATTTAATGGAATTATTAGAGCGCTGAGGGAGAAGGAATCCAAGCTCAAAGCGTCGAGAATGCATATTTATGTAAGAAGGGGTGGTCCAAACTATCAAACTGGTTTGGCAAAGATGCGTGCATTAGGAGATGAACTTGGTGTTCCTCTTGAGGTTTATGGGCCTGAGGCAACTATGACAGGAATTTGTAAGGAAGCAATTGATTGCATCATGAGTGAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

423

Amino Acids

46.65

Weight (kDa)

5.29

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Citrate_synth_N PF24948 2 - 230 2e-73 ATP-citrate synthase ATP-grasp domain
ATP-grasp_2 PF08442 6 - 203 8.7e-18 ATP-grasp domain
Citrate_bind PF16114 241 - 417 1.8e-84 ATP citrate lyase citrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 775
AccB1I GGYRCC 1 cut(s) 298
AccI GTMKAC 1 cut(s) 155
AccII CGCG 3 cut(s) 7, 198, 218
AciI CCGC 4 cut(s) 7, 198, 494, 665
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 2 cut(s) 785, 1230
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 1 cut(s) 26
AfeI AGCGCT 1 cut(s) 1071
AgsI TTSAA 4 cut(s) 58, 422, 673, 682
AhdI GACNNNNNGTC 1 cut(s) 167
AjnI CCWGG 1 cut(s) 588
AluBI AGCT 8 cut(s) 393, 585, 652, 830, 920, 1001, 1025, 1093
AluI AGCT 8 cut(s) 393, 585, 652, 830, 920, 1001, 1025, 1093
Alw21I GWGCWC 4 cut(s) 386, 493, 922, 1003
Alw26I GTCTC 4 cut(s) 31, 134, 269, 474
Ama87I CYCGRG 1 cut(s) 377
Aor51HI AGCGCT 1 cut(s) 1071
AoxI GGCC 3 cut(s) 129, 208, 1208
ApeKI GCWGC 3 cut(s) 49, 259, 1043
ApoI RAATTY 2 cut(s) 785, 1230
ArsI GACNNNNNNTTYG 4 cut(s) 133, 165, 170, 202
Asp700I GAANNNNTTC 1 cut(s) 627
AspLEI GCGC 5 cut(s) 7, 198, 220, 301, 1072
AspS9I GGNCC 3 cut(s) 819, 1133, 1208
AsuHPI GGTGA 3 cut(s) 112, 112, 632
AsuII TTCGAA 1 cut(s) 32
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 2 cut(s) 819, 1133
AxyI CCTNAGG 1 cut(s) 1212
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 2 cut(s) 922, 1003
Bbv12I GWGCWC 4 cut(s) 386, 493, 922, 1003
BbvCI CCTCAGC 1 cut(s) 1073
BbvI GCAGC 3 cut(s) 61, 271, 1030
BccI CCATC 3 cut(s) 277, 466, 980
BceAI ACGGC 2 cut(s) 310, 536
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 1 cut(s) 590
BciVI GTATCC 1 cut(s) 376
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 4 cut(s) 31, 134, 269, 474
BfaI CTAG 1 cut(s) 653
BfoI RGCGCY 2 cut(s) 302, 1073
BfuI GTATCC 1 cut(s) 376
BisI GCNGC 4 cut(s) 50, 260, 666, 1044
BlsI GCNGC 4 cut(s) 51, 261, 667, 1045
Bme1390I CCNGG 1 cut(s) 590
Bme18I GGWCC 2 cut(s) 819, 1133
BmeRI GACNNNNNGTC 1 cut(s) 167
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 3 cut(s) 819, 1133, 1208
BmiI GGNNCC 2 cut(s) 300, 324
BmrFI CCNGG 1 cut(s) 590
BmsI GCATC 4 cut(s) 822, 893, 1152, 1263
BpmI CTGGAG 3 cut(s) 65, 536, 852
Bpu10I CCTNAGC 3 cut(s) 96, 237, 1073
Bpu14I TTCGAA 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 1217
BsaHI GRCGYC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 609, 822
Bse1I ACTGG 2 cut(s) 519, 1153
Bse21I CCTNAGG 1 cut(s) 1212
BseBI CCWGG 1 cut(s) 590
BseDI CCNNGG 2 cut(s) 609, 822
BseGI GGATG 2 cut(s) 458, 766
BseMII CTCAG 4 cut(s) 110, 251, 1064, 1203
BseNI ACTGG 2 cut(s) 519, 1153
BseXI GCAGC 3 cut(s) 61, 271, 1030
Bsh1236I CGCG 3 cut(s) 7, 198, 218
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 3 cut(s) 131, 210, 1210
BshNI GGYRCC 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 4 cut(s) 386, 493, 922, 1003
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 2 cut(s) 69, 176
BslFI GGGAC 1 cut(s) 446
BsmAI GTCTC 4 cut(s) 31, 134, 269, 474
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 446
BsmI GAATGC 1 cut(s) 1113
BsnI GGCC 3 cut(s) 131, 210, 1210
BsoBI CYCGRG 1 cut(s) 377
Bsp119I TTCGAA 1 cut(s) 32
Bsp1286I GDGCHC 4 cut(s) 386, 493, 922, 1003
Bsp143I GATC 3 cut(s) 15, 84, 340
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 4 cut(s) 7, 198, 494, 665
BspANI GGCC 3 cut(s) 131, 210, 1210
BspCNI CTCAG 4 cut(s) 109, 250, 1065, 1204
BspFNI CGCG 3 cut(s) 7, 198, 218
BspHI TCATGA 2 cut(s) 337, 1257
BspLI GGNNCC 2 cut(s) 300, 324
BspQI GCTCTTC 1 cut(s) 1008
BspT104I TTCGAA 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 298
BsrI ACTGG 2 cut(s) 519, 1153
BssECI CCNNGG 2 cut(s) 609, 822
BssMI GATC 3 cut(s) 15, 84, 340
BssNI GRCGYC 1 cut(s) 299
BssT1I CCWWGG 2 cut(s) 609, 822
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 2 cut(s) 793, 865
Bst6I CTCTTC 6 cut(s) 29, 197, 417, 513, 927, 1008
BstACI GRCGYC 1 cut(s) 299
BstBI TTCGAA 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 844, 1167
BstDEI CTNAG 4 cut(s) 96, 237, 1073, 1212
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 2 cut(s) 458, 766
BstFNI CGCG 3 cut(s) 7, 198, 218
BstH2I RGCGCY 2 cut(s) 302, 1073
BstHHI GCGC 5 cut(s) 7, 198, 220, 301, 1072
BstKTI GATC 3 cut(s) 18, 87, 343
BstMAI GTCTC 4 cut(s) 31, 134, 269, 474
BstMBI GATC 3 cut(s) 15, 84, 340
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 7 cut(s) 49, 58, 181, 242, 331, 390, 557
BstNI CCWGG 1 cut(s) 590
BstNSI RCATGY 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 588
BstUI CGCG 3 cut(s) 7, 198, 218
BstV1I GCAGC 3 cut(s) 61, 271, 1030
Bsu36I CCTNAGG 1 cut(s) 1212
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 3 cut(s) 131, 210, 1210
BtgI CCRYGG 1 cut(s) 822
BtsCI GGATG 2 cut(s) 458, 766
BtsI GCAGTG 2 cut(s) 660, 707
BtsIMutI CAGTG 4 cut(s) 512, 660, 707, 798
Cac8I GCNNGC 2 cut(s) 844, 1167
CciI TCATGA 2 cut(s) 337, 1257
CfoI GCGC 5 cut(s) 7, 198, 220, 301, 1072
Cfr13I GGNCC 3 cut(s) 819, 1133, 1208
CseI GACGC 4 cut(s) 251, 765, 819, 1089
Csp6I GTAC 1 cut(s) 25
CviAII CATG 6 cut(s) 144, 181, 232, 338, 823, 1258
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 4 cut(s) 96, 237, 1073, 1212
DinI GGCGCC 1 cut(s) 300
DpnI GATC 3 cut(s) 17, 86, 342
DpnII GATC 3 cut(s) 15, 84, 340
DrdI GACNNNNNNGTC 1 cut(s) 775
DriI GACNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 775
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 6 cut(s) 29, 197, 417, 513, 927, 1008
Eam1105I GACNNNNNGTC 1 cut(s) 167
EarI CTCTTC 6 cut(s) 29, 197, 417, 513, 927, 1008
Ecl136II GAGCTC 2 cut(s) 920, 1001
Eco130I CCWWGG 2 cut(s) 609, 822
Eco24I GRGCYC 2 cut(s) 922, 1003
Eco47I GGWCC 2 cut(s) 819, 1133
Eco47III AGCGCT 1 cut(s) 1071
Eco53kI GAGCTC 2 cut(s) 920, 1001
Eco57I CTGAAG 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 1212
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 2 cut(s) 920, 1001
EcoRII CCWGG 1 cut(s) 588
EcoT14I CCWWGG 2 cut(s) 609, 822
EcoT22I ATGCAT 2 cut(s) 886, 1113
EcoT38I GRGCYC 2 cut(s) 922, 1003
EgeI GGCGCC 1 cut(s) 300
EheI GGCGCC 1 cut(s) 300
ErhI CCWWGG 2 cut(s) 609, 822
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 6 cut(s) 147, 184, 235, 341, 826, 1261
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 1 cut(s) 446
FatI CATG 6 cut(s) 143, 180, 231, 337, 822, 1257
FauI CCCGC 1 cut(s) 191
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 4 cut(s) 50, 260, 666, 1044
FokI GGATG 2 cut(s) 445, 773
FriOI GRGCYC 2 cut(s) 922, 1003
Fsp4HI GCNGC 4 cut(s) 50, 260, 666, 1044
FspBI CTAG 1 cut(s) 653
GlaI GCGC 5 cut(s) 6, 197, 219, 300, 1071
GluI GCNGC 4 cut(s) 50, 260, 666, 1044
GsuI CTGGAG 3 cut(s) 65, 536, 852
HaeII RGCGCY 2 cut(s) 302, 1073
HaeIII GGCC 3 cut(s) 131, 210, 1210
HapII CCGG 2 cut(s) 69, 176
HgaI GACGC 4 cut(s) 251, 765, 819, 1089
HhaI GCGC 5 cut(s) 7, 198, 220, 301, 1072
Hin1I GRCGYC 1 cut(s) 299
Hin1II CATG 6 cut(s) 147, 184, 235, 341, 826, 1261
Hin6I GCGC 5 cut(s) 5, 196, 218, 299, 1070
HinP1I GCGC 5 cut(s) 5, 196, 218, 299, 1070
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HindIII AAGCTT 1 cut(s) 391
HinfI GANTC 5 cut(s) 29, 162, 799, 1085, 1265
HpaII CCGG 2 cut(s) 69, 176
HphI GGTGA 3 cut(s) 112, 112, 632
Hpy166II GTNNAC 3 cut(s) 156, 606, 623
Hpy188I TCNGA 6 cut(s) 20, 370, 400, 463, 889, 1008
Hpy8I GTNNAC 3 cut(s) 156, 606, 623
Hpy99I CGWCG 5 cut(s) 157, 170, 218, 313, 1105
HpyAV CCTTC 2 cut(s) 1075, 1119
HpyCH4III ACNGT 2 cut(s) 793, 865
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 9 cut(s) 842, 884, 905, 943, 950, 971, 1111, 1169, 1254
HpyF10VI GCNNNNNNNGC 7 cut(s) 49, 58, 181, 242, 331, 390, 557
HpyF3I CTNAG 4 cut(s) 96, 237, 1073, 1212
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 1 cut(s) 299
Hsp92II CATG 6 cut(s) 147, 184, 235, 341, 826, 1261
HspAI GCGC 5 cut(s) 5, 196, 218, 299, 1070
KasI GGCGCC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 3 cut(s) 15, 84, 340
LguI GCTCTTC 1 cut(s) 1008
LmnI GCTCC 6 cut(s) 322, 381, 496, 649, 917, 925
Lsp1109I GCAGC 3 cut(s) 61, 271, 1030
LweI GCATC 4 cut(s) 822, 893, 1152, 1263
MaeI CTAG 1 cut(s) 653
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 100
MalI GATC 3 cut(s) 17, 86, 342
MboI GATC 3 cut(s) 15, 84, 340
MfeI CAATTG 1 cut(s) 1245
MhlI GDGCHC 4 cut(s) 386, 493, 922, 1003
MlsI TGGCCA 1 cut(s) 131
MluNI TGGCCA 1 cut(s) 131
Mly113I GGCGCC 1 cut(s) 299
MlyI GAGTC 1 cut(s) 156
MmeI TCCRAC 2 cut(s) 758, 847
Mox20I TGGCCA 1 cut(s) 131
Mph1103I ATGCAT 2 cut(s) 886, 1113
MroXI GAANNNNTTC 1 cut(s) 627
MscI TGGCCA 1 cut(s) 131
MseI TTAA 4 cut(s) 615, 728, 782, 1053
MslI CAYNNNNRTG 1 cut(s) 1116
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 496
MspI CCGG 2 cut(s) 69, 176
MspR9I CCNGG 1 cut(s) 590
MunI CAATTG 1 cut(s) 1245
Mva1269I GAATGC 1 cut(s) 1113
MvaI CCWGG 1 cut(s) 590
MvnI CGCG 3 cut(s) 7, 198, 218
MwoI GCNNNNNNNGC 7 cut(s) 49, 58, 181, 242, 331, 390, 557
NarI GGCGCC 1 cut(s) 299
NcoI CCATGG 1 cut(s) 822
NdeII GATC 3 cut(s) 15, 84, 340
NlaIII CATG 6 cut(s) 147, 184, 235, 341, 826, 1261
NlaIV GGNNCC 2 cut(s) 300, 324
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 1 cut(s) 100
NsiI ATGCAT 2 cut(s) 886, 1113
NspI RCATGY 1 cut(s) 184
NspV TTCGAA 1 cut(s) 32
PagI TCATGA 2 cut(s) 337, 1257
PciSI GCTCTTC 1 cut(s) 1008
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PctI GAATGC 1 cut(s) 1113
PdmI GAANNNNTTC 1 cut(s) 627
PfeI GAWTC 4 cut(s) 29, 799, 1085, 1265
PflFI GACNNNGTC 2 cut(s) 251, 437
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 4 cut(s) 51, 261, 667, 1045
PleI GAGTC 1 cut(s) 156
PluTI GGCGCC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 156
Psp124BI GAGCTC 2 cut(s) 922, 1003
Psp6I CCWGG 1 cut(s) 588
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 2 cut(s) 300, 324
PspPI GGNCC 3 cut(s) 819, 1133, 1208
PsyI GACNNNGTC 2 cut(s) 251, 437
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 1116
SacI GAGCTC 2 cut(s) 922, 1003
SalI GTCGAC 1 cut(s) 154
SapI GCTCTTC 1 cut(s) 1008
SaqAI TTAA 4 cut(s) 615, 728, 782, 1053
SatI GCNGC 4 cut(s) 50, 260, 666, 1044
Sau3AI GATC 3 cut(s) 15, 84, 340
Sau96I GGNCC 3 cut(s) 819, 1133, 1208
SchI GAGTC 1 cut(s) 156
ScrFI CCNGG 1 cut(s) 590
SduI GDGCHC 4 cut(s) 386, 493, 922, 1003
SfaNI GCATC 4 cut(s) 822, 893, 1152, 1263
SfoI GGCGCC 1 cut(s) 300
SfuI TTCGAA 1 cut(s) 32
SinI GGWCC 2 cut(s) 819, 1133
SmiMI CAYNNNNRTG 1 cut(s) 1116
SmlI CTYRAG 1 cut(s) 1196
SmoI CTYRAG 1 cut(s) 1196
SsiI CCGC 4 cut(s) 7, 198, 494, 665
SspDI GGCGCC 1 cut(s) 298
SspMI CTAG 1 cut(s) 653
SstI GAGCTC 2 cut(s) 922, 1003
StyD4I CCNGG 1 cut(s) 588
StyI CCWWGG 2 cut(s) 609, 822
TaaI ACNGT 2 cut(s) 793, 865
TaiI ACGT 1 cut(s) 314
TaqI TCGA 4 cut(s) 32, 155, 359, 1103
TaqII GACCGA 1 cut(s) 128
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 4 cut(s) 29, 799, 1085, 1265
Tru1I TTAA 4 cut(s) 615, 728, 782, 1053
Tru9I TTAA 4 cut(s) 615, 728, 782, 1053
TscAI CASTG 4 cut(s) 519, 667, 714, 798
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 3 cut(s) 49, 259, 1043
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 6 cut(s) 532, 611, 740, 777, 942, 1194
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 4 cut(s) 519, 667, 714, 798
Tth111I GACNNNGTC 2 cut(s) 251, 437
VpaK11BI GGWCC 2 cut(s) 819, 1133
XapI RAATTY 2 cut(s) 785, 1230
XceI RCATGY 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 627
XspI CTAG 1 cut(s) 653
Zsp2I ATGCAT 2 cut(s) 886, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.