Rorug06G0459800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000995
Physical Location & Seq
Reverse (-)
58655328 .. 58655753
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0459800.1

Sequence Viewer

Length: 426 bp
ATGCCACGGTGGCGCAACCCGGACAAACCTTTCGAAGACCGGGTGGAGCGAGCCATCCACAAGCACATTCGCCTCCTGCACCGCTCTGGTTCCAGGTTCTTCGTTTTAGGACACACCAAAAATGTGTACACTGTCACATTGTCCACCGAGTCAACATGCTCGTGCCCTGACGTTGTCTCCCCCTGCAAGCACCTGCTGTTTGTGTATCTCCAAGTATTGGGTGTTTGTATCGACACCCGTTGTGTTAGGAGGAGTGCACTCCATCCGAGCGAGGTGAGTTGGCTGTTGGGGTTGTCCATGTCGCGCGCATCACTGGCTGAGGATAGTGTCCAACAGTGGTTTCATCAGCTCCATCCTCTTGGAAAGAAACAGCAGCAGCAGCAGCAGGAGAGGCCTTTCTCATCGAGGCCAAGCCTAAGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

16.3

Weight (kDa)

9.72

Isoelectric Point (pI)

70.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 43 - 71 3.7e-06 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 201
Acc36I ACCTGC 1 cut(s) 201
AccB7I CCANNNNNTGG 1 cut(s) 217
AccBSI CCGCTC 1 cut(s) 84
AccII CGCG 2 cut(s) 304, 306
AciI CCGC 1 cut(s) 82
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 217
AjnI CCWGG 1 cut(s) 92
AjuI GAANNNNNNNTTGG 2 cut(s) 324, 356
AluBI AGCT 1 cut(s) 349
AluI AGCT 1 cut(s) 349
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 1 cut(s) 181
Alw44I GTGCAC 1 cut(s) 255
AoxI GGCC 2 cut(s) 392, 407
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 4 cut(s) 373, 376, 379, 382
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AspLEI GCGC 3 cut(s) 15, 306, 308
AsuC2I CCSGG 2 cut(s) 20, 41
AsuHPI GGTGA 1 cut(s) 286
AsuII TTCGAA 1 cut(s) 33
BaeGI GKGCMC 2 cut(s) 167, 259
BauI CACGAG 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 259
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 4 cut(s) 385, 388, 391, 394
BccI CCATC 3 cut(s) 62, 270, 360
BciT130I CCWGG 1 cut(s) 94
BcnI CCSGG 2 cut(s) 20, 41
BcoDI GTCTC 1 cut(s) 181
BfuAI ACCTGC 1 cut(s) 201
BglI GCCNNNNNGGC 1 cut(s) 10
BisI GCNGC 4 cut(s) 374, 377, 380, 383
BlsI GCNGC 4 cut(s) 375, 378, 381, 384
Bme1390I CCNGG 3 cut(s) 20, 41, 94
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 3 cut(s) 20, 41, 94
BmsI GCATC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 42
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 33
BpuMI CCSGG 2 cut(s) 20, 41
BsaJI CCNNGG 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 3 cut(s) 54, 262, 352
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 1 cut(s) 318
BsePI GCGCGC 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 265
BseSI GKGCMC 2 cut(s) 167, 259
BseXI GCAGC 4 cut(s) 385, 388, 391, 394
BsgI GTGCAG 1 cut(s) 62
Bsh1236I CGCG 2 cut(s) 304, 306
BshFI GGCC 2 cut(s) 394, 409
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 2 cut(s) 20, 40
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 181
BsnI GGCC 2 cut(s) 394, 409
Bsp119I TTCGAA 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 167, 259
Bsp1407I TGTACA 1 cut(s) 126
BspACI CCGC 1 cut(s) 82
BspANI GGCC 2 cut(s) 394, 409
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 2 cut(s) 304, 306
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 201
BspT104I TTCGAA 1 cut(s) 33
BsrBI CCGCTC 1 cut(s) 84
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 1 cut(s) 5
BssHII GCGCGC 1 cut(s) 304
BssSI CACGAG 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 3 cut(s) 9, 133, 336
BstAUI TGTACA 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 51, 188, 306
BstDEI CTNAG 2 cut(s) 318, 416
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 3 cut(s) 54, 262, 352
BstFNI CGCG 2 cut(s) 304, 306
BstHHI GCGC 3 cut(s) 15, 306, 308
BstMAI GTCTC 1 cut(s) 181
BstMWI GCNNNNNNNGC 5 cut(s) 10, 314, 379, 382, 391
BstNI CCWGG 1 cut(s) 94
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 3 cut(s) 18, 39, 92
BstSLI GKGCMC 2 cut(s) 167, 259
BstUI CGCG 2 cut(s) 304, 306
BstV1I GCAGC 4 cut(s) 385, 388, 391, 394
BstV2I GAAGAC 1 cut(s) 42
BstXI CCANNNNNNTGG 1 cut(s) 359
BsuRI GGCC 2 cut(s) 394, 409
BtgI CCRYGG 1 cut(s) 5
BtsCI GGATG 3 cut(s) 54, 262, 352
BtsIMutI CAGTG 3 cut(s) 129, 311, 341
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 3 cut(s) 51, 188, 306
CfoI GCGC 3 cut(s) 15, 306, 308
Csp6I GTAC 1 cut(s) 127
CviAII CATG 2 cut(s) 156, 298
CviJI RGCY 7 cut(s) 53, 283, 317, 349, 394, 409, 414
CviKI_1 RGCY 7 cut(s) 53, 283, 317, 349, 394, 409, 414
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 2 cut(s) 318, 416
Eco147I AGGCCT 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 92
FaeI CATG 2 cut(s) 159, 301
FaiI YATR 2 cut(s) 157, 299
FatI CATG 2 cut(s) 155, 297
Fnu4HI GCNGC 4 cut(s) 374, 377, 380, 383
FokI GGATG 3 cut(s) 41, 249, 339
Fsp4HI GCNGC 4 cut(s) 374, 377, 380, 383
GlaI GCGC 3 cut(s) 14, 305, 307
GluI GCNGC 4 cut(s) 374, 377, 380, 383
HaeIII GGCC 2 cut(s) 394, 409
HapII CCGG 2 cut(s) 20, 40
HhaI GCGC 3 cut(s) 15, 306, 308
Hin1II CATG 2 cut(s) 159, 301
Hin6I GCGC 3 cut(s) 13, 304, 306
HinP1I GCGC 3 cut(s) 13, 304, 306
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HinfI GANTC 1 cut(s) 149
HpaII CCGG 2 cut(s) 20, 40
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 5 cut(s) 127, 129, 144, 153, 257
Hpy188I TCNGA 1 cut(s) 267
Hpy8I GTNNAC 5 cut(s) 127, 129, 144, 153, 257
HpyCH4III ACNGT 3 cut(s) 9, 133, 336
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 79, 186, 257
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 314, 379, 382, 391
HpyF3I CTNAG 2 cut(s) 318, 416
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 2 cut(s) 159, 301
HspAI GCGC 3 cut(s) 13, 304, 306
LmnI GCTCC 2 cut(s) 46, 354
Lsp1109I GCAGC 4 cut(s) 385, 388, 391, 394
LweI GCATC 1 cut(s) 317
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 133
MbiI CCGCTC 1 cut(s) 84
MboII GAAGA 2 cut(s) 47, 91
MhlI GDGCHC 2 cut(s) 167, 259
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 7 cut(s) 83, 243, 265, 313, 366, 384, 399
MslI CAYNNNNRTG 1 cut(s) 160
MspI CCGG 2 cut(s) 20, 40
MspR9I CCNGG 3 cut(s) 20, 41, 94
MvaI CCWGG 1 cut(s) 94
MvnI CGCG 2 cut(s) 304, 306
MwoI GCNNNNNNNGC 5 cut(s) 10, 314, 379, 382, 391
NciI CCSGG 2 cut(s) 20, 41
NlaIII CATG 2 cut(s) 159, 301
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 133
NspI RCATGY 1 cut(s) 159
NspV TTCGAA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 201
PauI GCGCGC 1 cut(s) 304
PceI AGGCCT 1 cut(s) 394
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 4 cut(s) 375, 378, 381, 384
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 173
PteI GCGCGC 1 cut(s) 304
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 160
SatI GCNGC 4 cut(s) 374, 377, 380, 383
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 3 cut(s) 20, 41, 94
SduI GDGCHC 2 cut(s) 167, 259
SetI ASST 6 cut(s) 31, 98, 174, 195, 276, 351
SfaNI GCATC 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 160
SseBI AGGCCT 1 cut(s) 394
SsiI CCGC 1 cut(s) 82
StuI AGGCCT 1 cut(s) 394
StyD4I CCNGG 3 cut(s) 18, 39, 92
TaaI ACNGT 3 cut(s) 9, 133, 336
TaiI ACGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 33, 231, 404
TatI WGTACW 1 cut(s) 126
TscAI CASTG 3 cut(s) 136, 318, 341
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 4 cut(s) 373, 376, 379, 382
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 332
TspRI CASTG 3 cut(s) 136, 318, 341
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 1 cut(s) 217
VneI GTGCAC 1 cut(s) 255
XceI RCATGY 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.