MD06G1205800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000995
Physical Location & Seq
Forward (+)
34053797 .. 34057811
4015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1205800.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGATGGCCATTCAACCATGGTCTTCCAGCCTGTTTACAATTCCTTCAAGTTCAAAAATTCCGAAGCCATGAAGGGATTGAAGGTGGAAAAAAGTACTAGTTCAAACGATGATAATCTGTTCATTTCTGATTTGCAAAGTCAAGACAGTGAGGTGGTTCTGAATCCACATCCAGATGATAGTCAGAATATGCGCGCTATAAAGGAAGCCGTGACAGCTGGTGATTGCGCAGCTTGGAAGGATTCTGCTAGAAAGGCTGCAATGGACTATGACTTGCCAGAGTTGGTTGCATTCCTTCAAGGGAGCAGCAACTACCAGTTGTTCAAGGACATCTGCATTGACAAGGAAGTTCGATGCAAAGATAAGTGTTCCGTAGAAAACTGTGAATTCTATCATAGTAGCACTGCTTGCATGCTTGATTCTGATCCGGACAGCGAGAGCGAGTCAATTAGAGAATCTCTGGACTCGGAGTCATCCACTTCAACGGATGAGAATGACTGCAAAAAGGATGATCTATCAAATGATCATTCAATCAAAAAGATCATATCCGAAAAAGTGTTTTTGGTTGGACAGGGTCCAAGTAACGCAGTCTTGGCCGCAAACTCAATGGGATTATCTATAACTATGGGTGACAACAGACGGACAAGCAATGCTTCTGTAAAAGGTGACCTAGAAAATACAAGCGGAAGCGCAGAGTTTCATGCCCCAGAAATGGAAAACATGTTGAGGCATGATTCGTCCGAAGATGGGATGTCAGATGGCCTAGCAGAATCGAGTCAACGACACCAGCATTGCAGCAGAGGATCCAGTTCCTCTGCATACGAACTTCCTTCGGGCTCGTTTTCGTACTCAGGAAGAATACCAGGTTTTGGCAGCATCTCTTTTCGGTCGAGCAGCAGCACAACTAGCTCTCGGTCTTTCTCTTTTCCCATATTATCCTCGGAATGGAATGGCAGCCCGGCGAGAATGGGGAGCGCTGACCATAGACAATTACACCGGCACCGGCACTGGGGGATGCGATTTCTCTGCTGCAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

347

Amino Acids

38.11

Weight (kDa)

5.24

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 229
AccB1I GGYRCC 1 cut(s) 999
AccB7I CCANNNNNTGG 1 cut(s) 869
AccII CGCG 1 cut(s) 195
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 2 cut(s) 597, 684
AclWI GGATC 3 cut(s) 419, 798, 811
AcoI YGGCCR 2 cut(s) 7, 594
AcsI RAATTY 3 cut(s) 58, 386, 1034
AfaI GTAC 2 cut(s) 97, 848
AfeI AGCGCT 1 cut(s) 976
AfiI CCNNNNNNNGG 5 cut(s) 712, 747, 869, 945, 1009
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 9 cut(s) 15, 49, 55, 82, 105, 299, 325, 483, 531
AhdI GACNNNNNGTC 1 cut(s) 469
AhlI ACTAGT 1 cut(s) 98
AjnI CCWGG 1 cut(s) 862
AluBI AGCT 3 cut(s) 218, 233, 909
AluI AGCT 3 cut(s) 218, 233, 909
AlwI GGATC 3 cut(s) 419, 798, 811
Aor13HI TCCGGA 1 cut(s) 427
Aor51HI AGCGCT 1 cut(s) 976
AoxI GGCC 3 cut(s) 7, 594, 760
ApeKI GCWGC 9 cut(s) 230, 257, 306, 795, 873, 894, 897, 954, 1029
ApoI RAATTY 3 cut(s) 58, 386, 1034
AspLEI GCGC 5 cut(s) 195, 197, 230, 692, 977
AspS9I GGNCC 1 cut(s) 575
AsuC2I CCSGG 1 cut(s) 959
AsuHPI GGTGA 3 cut(s) 233, 641, 677
AvaII GGWCC 1 cut(s) 575
BalI TGGCCA 1 cut(s) 9
BamHI GGATCC 1 cut(s) 803
BanI GGYRCC 1 cut(s) 999
BanII GRGCYC 1 cut(s) 839
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 9 cut(s) 242, 244, 318, 807, 885, 906, 909, 966, 1016
BccI CCATC 2 cut(s) 740, 752
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 1 cut(s) 1008
BciT130I CCWGG 1 cut(s) 864
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 1 cut(s) 959
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 5 cut(s) 99, 249, 671, 764, 906
BfoI RGCGCY 1 cut(s) 978
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 2 cut(s) 864, 959
Bme18I GGWCC 1 cut(s) 575
BmeRI GACNNNNNGTC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 3 cut(s) 576, 805, 1001
BmrFI CCNGG 2 cut(s) 864, 959
BmrI ACTGGG 1 cut(s) 1018
BmsI GCATC 3 cut(s) 344, 885, 1005
BmuI ACTGGG 1 cut(s) 1018
BpiI GAAGAC 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 959
BsaBI GATNNNNATC 2 cut(s) 114, 423
BsaJI CCNNGG 2 cut(s) 18, 939
BsaWI WCCGGW 1 cut(s) 427
Bsc4I CCNNNNNNNGG 5 cut(s) 712, 747, 869, 945, 1009
Bse118I RCCGGY 2 cut(s) 996, 1002
Bse1I ACTGG 3 cut(s) 316, 807, 1013
Bse3DI GCAATG 3 cut(s) 267, 655, 790
Bse8I GATNNNNATC 2 cut(s) 114, 423
BseAI TCCGGA 1 cut(s) 427
BseBI CCWGG 1 cut(s) 864
BseDI CCNNGG 2 cut(s) 18, 939
BseGI GGATG 7 cut(s) 10, 169, 473, 493, 514, 756, 1020
BseJI GATNNNNATC 2 cut(s) 114, 423
BseLI CCNNNNNNNGG 5 cut(s) 712, 747, 869, 945, 1009
BseMI GCAATG 3 cut(s) 267, 655, 790
BseMII CTCAG 1 cut(s) 864
BseNI ACTGG 3 cut(s) 316, 807, 1013
BsePI GCGCGC 1 cut(s) 193
BseXI GCAGC 9 cut(s) 242, 244, 318, 807, 885, 906, 909, 966, 1016
Bsh1236I CGCG 1 cut(s) 195
Bsh1285I CGRYCG 1 cut(s) 890
BshFI GGCC 3 cut(s) 9, 596, 762
BshNI GGYRCC 1 cut(s) 999
BsiEI CGRYCG 1 cut(s) 890
BsiSI CCGG 4 cut(s) 428, 959, 997, 1003
BslI CCNNNNNNNGG 5 cut(s) 712, 747, 869, 945, 1009
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 3 cut(s) 9, 596, 762
Bsp1286I GDGCHC 1 cut(s) 839
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 5 cut(s) 424, 511, 523, 540, 803
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 2 cut(s) 597, 684
BspANI GGCC 3 cut(s) 9, 596, 762
BspCNI CTCAG 1 cut(s) 863
BspEI TCCGGA 1 cut(s) 427
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 3 cut(s) 576, 805, 1001
BspPI GGATC 3 cut(s) 419, 798, 811
BspT107I GGYRCC 1 cut(s) 999
BsrDI GCAATG 3 cut(s) 267, 655, 790
BsrFI RCCGGY 2 cut(s) 996, 1002
BsrI ACTGG 3 cut(s) 316, 807, 1013
BssAI RCCGGY 2 cut(s) 996, 1002
BssECI CCNNGG 2 cut(s) 18, 939
BssHII GCGCGC 1 cut(s) 193
BssMI GATC 5 cut(s) 424, 511, 523, 540, 803
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 864
Bst4CI ACNGT 2 cut(s) 149, 383
BstAPI GCANNNNNTGC 1 cut(s) 408
BstC8I GCNNGC 3 cut(s) 195, 409, 413
BstDEI CTNAG 1 cut(s) 850
BstDSI CCRYGG 1 cut(s) 18
BstEII GGTNACC 1 cut(s) 665
BstF5I GGATG 7 cut(s) 10, 169, 473, 493, 514, 756, 1020
BstFNI CGCG 1 cut(s) 195
BstH2I RGCGCY 1 cut(s) 978
BstHHI GCGC 5 cut(s) 195, 197, 230, 692, 977
BstKTI GATC 5 cut(s) 427, 514, 526, 543, 806
BstMBI GATC 5 cut(s) 424, 511, 523, 540, 803
BstMCI CGRYCG 1 cut(s) 890
BstMWI GCNNNNNNNGC 5 cut(s) 215, 254, 408, 593, 906
BstNI CCWGG 1 cut(s) 864
BstNSI RCATGY 2 cut(s) 415, 724
BstPI GGTNACC 1 cut(s) 665
BstSCI CCNGG 2 cut(s) 862, 957
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 9 cut(s) 242, 244, 318, 807, 885, 906, 909, 966, 1016
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
BsuRI GGCC 3 cut(s) 9, 596, 762
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 7 cut(s) 10, 169, 473, 493, 514, 756, 1020
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 3 cut(s) 154, 402, 1006
Cac8I GCNNGC 3 cut(s) 195, 409, 413
CfoI GCGC 5 cut(s) 195, 197, 230, 692, 977
Cfr10I RCCGGY 2 cut(s) 996, 1002
Cfr13I GGNCC 1 cut(s) 575
CsiI ACCWGGT 1 cut(s) 862
Csp6I GTAC 2 cut(s) 96, 847
CviAII CATG 6 cut(s) 19, 70, 412, 701, 721, 731
CviQI GTAC 2 cut(s) 96, 847
DdeI CTNAG 1 cut(s) 850
DpnI GATC 5 cut(s) 426, 513, 525, 542, 805
DpnII GATC 5 cut(s) 424, 511, 523, 540, 803
DriI GACNNNNNGTC 1 cut(s) 469
EaeI YGGCCR 2 cut(s) 7, 594
Eam1105I GACNNNNNGTC 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 839
Eco47I GGWCC 1 cut(s) 575
Eco47III AGCGCT 1 cut(s) 976
Eco91I GGTNACC 1 cut(s) 665
EcoO65I GGTNACC 1 cut(s) 665
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 862
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 839
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 6 cut(s) 22, 73, 415, 704, 724, 734
FalI AAGNNNNNCTT 2 cut(s) 637, 669
FatI CATG 6 cut(s) 18, 69, 411, 700, 720, 730
FbaI TGATCA 1 cut(s) 523
FokI GGATG 7 cut(s) 17, 156, 460, 500, 521, 763, 1027
FriOI GRGCYC 1 cut(s) 839
FspBI CTAG 5 cut(s) 99, 249, 671, 764, 906
FspI TGCGCA 1 cut(s) 229
GlaI GCGC 5 cut(s) 194, 196, 229, 691, 976
HaeII RGCGCY 1 cut(s) 978
HaeIII GGCC 3 cut(s) 9, 596, 762
HapII CCGG 4 cut(s) 428, 959, 997, 1003
HhaI GCGC 5 cut(s) 195, 197, 230, 692, 977
Hin1II CATG 6 cut(s) 22, 73, 415, 704, 724, 734
Hin6I GCGC 5 cut(s) 193, 195, 228, 690, 975
HinP1I GCGC 5 cut(s) 193, 195, 228, 690, 975
HincII GTYRAC 1 cut(s) 779
HindII GTYRAC 1 cut(s) 779
HpaII CCGG 4 cut(s) 428, 959, 997, 1003
HphI GGTGA 3 cut(s) 233, 641, 677
Hpy166II GTNNAC 2 cut(s) 37, 779
Hpy188III TCNNGA 5 cut(s) 143, 173, 428, 461, 852
Hpy8I GTNNAC 2 cut(s) 37, 779
HpyAV CCTTC 6 cut(s) 55, 67, 76, 232, 305, 840
HpyCH4III ACNGT 2 cut(s) 149, 383
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 254, 408, 593, 906
HpyF3I CTNAG 1 cut(s) 850
Hsp92II CATG 6 cut(s) 22, 73, 415, 704, 724, 734
HspAI GCGC 5 cut(s) 193, 195, 228, 690, 975
Kpn2I TCCGGA 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 5 cut(s) 424, 511, 523, 540, 803
LmnI GCTCC 2 cut(s) 303, 972
Lsp1109I GCAGC 9 cut(s) 242, 244, 318, 807, 885, 906, 909, 966, 1016
LweI GCATC 3 cut(s) 344, 885, 1005
MabI ACCWGGT 1 cut(s) 862
MaeI CTAG 5 cut(s) 99, 249, 671, 764, 906
MaeIII GTNAC 4 cut(s) 211, 581, 629, 665
MalI GATC 5 cut(s) 426, 513, 525, 542, 805
MboI GATC 5 cut(s) 424, 511, 523, 540, 803
MboII GAAGA 3 cut(s) 16, 755, 867
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 1 cut(s) 839
MlsI TGGCCA 1 cut(s) 9
MluCI AATT 6 cut(s) 40, 58, 386, 447, 989, 1034
MluNI TGGCCA 1 cut(s) 9
MlyI GAGTC 4 cut(s) 452, 458, 479, 784
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 5 cut(s) 145, 720, 794, 823, 949
Mox20I TGGCCA 1 cut(s) 9
MroI TCCGGA 1 cut(s) 427
MscI TGGCCA 1 cut(s) 9
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 4 cut(s) 428, 959, 997, 1003
MspR9I CCNGG 2 cut(s) 864, 959
Mva1269I GAATGC 1 cut(s) 290
MvaI CCWGG 1 cut(s) 864
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 5 cut(s) 215, 254, 408, 593, 906
NciI CCSGG 1 cut(s) 959
NcoI CCATGG 1 cut(s) 18
NdeII GATC 5 cut(s) 424, 511, 523, 540, 803
NlaIII CATG 6 cut(s) 22, 73, 415, 704, 724, 734
NlaIV GGNNCC 3 cut(s) 576, 805, 1001
NmuCI GTSAC 3 cut(s) 211, 629, 665
NsbI TGCGCA 1 cut(s) 229
NspI RCATGY 2 cut(s) 415, 724
PaeI GCATGC 1 cut(s) 415
PauI GCGCGC 1 cut(s) 193
PciI ACATGT 1 cut(s) 720
PctI GAATGC 1 cut(s) 290
PfeI GAWTC 6 cut(s) 163, 242, 419, 455, 734, 770
PflFI GACNNNGTC 1 cut(s) 573
PflMI CCANNNNNTGG 1 cut(s) 869
PleI GAGTC 4 cut(s) 451, 458, 478, 783
PpsI GAGTC 4 cut(s) 451, 458, 478, 783
PscI ACATGT 1 cut(s) 720
Psp6I CCWGG 1 cut(s) 862
PspEI GGTNACC 1 cut(s) 665
PspGI CCWGG 1 cut(s) 862
PspN4I GGNNCC 3 cut(s) 576, 805, 1001
PspPI GGNCC 1 cut(s) 575
PsuI RGATCY 1 cut(s) 803
PsyI GACNNNGTC 1 cut(s) 573
PteI GCGCGC 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 2 cut(s) 97, 848
RsaNI GTAC 2 cut(s) 96, 847
RseI CAYNNNNRTG 1 cut(s) 174
Sau3AI GATC 5 cut(s) 424, 511, 523, 540, 803
Sau96I GGNCC 1 cut(s) 575
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 4 cut(s) 452, 458, 479, 784
ScrFI CCNGG 2 cut(s) 864, 959
SduI GDGCHC 1 cut(s) 839
SetI ASST 8 cut(s) 87, 156, 220, 235, 667, 672, 868, 911
SexAI ACCWGGT 1 cut(s) 862
SfaNI GCATC 3 cut(s) 344, 885, 1005
SinI GGWCC 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 174
SpeI ACTAGT 1 cut(s) 98
SphI GCATGC 1 cut(s) 415
Sse9I AATT 6 cut(s) 40, 58, 386, 447, 989, 1034
SsiI CCGC 2 cut(s) 597, 684
SspMI CTAG 5 cut(s) 99, 249, 671, 764, 906
StyD4I CCNGG 2 cut(s) 862, 957
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 149, 383
TaqI TCGA 3 cut(s) 352, 773, 890
TaqII GACCGA 2 cut(s) 876, 903
TasI AATT 6 cut(s) 40, 58, 386, 447, 989, 1034
TatI WGTACW 1 cut(s) 95
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 6 cut(s) 163, 242, 419, 455, 734, 770
TscAI CASTG 3 cut(s) 154, 409, 1013
TseFI GTSAC 3 cut(s) 211, 629, 665
TseI GCWGC 9 cut(s) 230, 257, 306, 795, 873, 894, 897, 954, 1029
Tsp45I GTSAC 3 cut(s) 211, 629, 665
TspDTI ATGAA 3 cut(s) 86, 112, 689
TspGWI ACGGA 3 cut(s) 361, 500, 655
TspRI CASTG 3 cut(s) 154, 409, 1013
Tth111I GACNNNGTC 1 cut(s) 573
Van91I CCANNNNNTGG 1 cut(s) 869
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 3 cut(s) 58, 386, 1034
XceI RCATGY 2 cut(s) 415, 724
XspI CTAG 5 cut(s) 99, 249, 671, 764, 906
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.