Rorug06G0459900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000995
Physical Location & Seq
Reverse (-)
58656376 .. 58657905
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0459900.1

Sequence Viewer

Length: 666 bp
ATGCCGCGGCCTAGCCGCTACAAGCCCTTCAATGACCGCTTGGAGCGAGCCCTCAACCAGCACATTCGCCTCCTGCACCGCTCCGGTGCCACCTTCTTCATCCTCGGCGACACGGGCAATGTGTACACTGCGACCATCACCACTTACCCTAAATGCAGCTGCCCTGACCCTGTAACACCATGCAAGCATTTGCTGTTTGTGTATCTCCAAGTGTTGGGTCTTTCAGCCGATGGTCTCAGGGACGGCACGTTCTCGCAGTGGGAGGTGCAGTGCATGCTGGGGCGGGACACGCTGCCTGAATCGCTGGCCGGGGAAAGCGTGCGGCAGTGGTTTCATCAGCTGTATGATTTTCAGGGCAGGCAGAGGCAGCAGGGGTCTTCTTCGTCGTCGAGGCCAAGAGTGGTGATTGAAGAGGGTACTTGCTGCCCTGTTTGTTTGGATGAGATGGGGAAGCAAGACAAAGTGGTGGCTTGTGGGACGTGTAGAAACCCGATACATGAGGAATGCTTCTTGAAGTGGAAGAGGAGCGCGAGGAAGAAGCCAGCTCATTGTGTGATGTGCAGGGCGAGATGGGGGTCGATCCAGCAGGAGCAGGAGAAGTACTTGAACTTGGCGGCTTACGCTAGTACGAGCGAGGAGGATGAGGACGAAGACGAATATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

25.31

Weight (kDa)

6.6

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 141 - 188 4.1e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 81
AccII CGCG 2 cut(s) 7, 530
AciI CCGC 8 cut(s) 5, 7, 16, 37, 79, 283, 322, 614
AclWI GGATC 1 cut(s) 574
AcoI YGGCCR 1 cut(s) 306
AfaI GTAC 4 cut(s) 125, 418, 602, 628
AfiI CCNNNNNNNGG 1 cut(s) 214
AflIII ACRYGT 1 cut(s) 479
AgsI TTSAA 4 cut(s) 31, 410, 514, 607
AjiI CACGTC 1 cut(s) 480
AloI GAACNNNNNNTCC 2 cut(s) 233, 265
AluBI AGCT 3 cut(s) 159, 340, 545
AluI AGCT 3 cut(s) 159, 340, 545
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 574
AoxI GGCC 3 cut(s) 8, 306, 392
ApeKI GCWGC 5 cut(s) 156, 159, 292, 367, 423
AspLEI GCGC 1 cut(s) 530
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 2 cut(s) 130, 415
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 369, 657
BbvI GCAGC 5 cut(s) 146, 168, 279, 379, 410
BccI CCATC 4 cut(s) 143, 224, 439, 564
BceAI ACGGC 1 cut(s) 259
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 2 cut(s) 12, 624
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 1 cut(s) 310
BmgBI CACGTC 1 cut(s) 480
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 310
BpiI GAAGAC 2 cut(s) 369, 657
BpuMI CCSGG 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 3 cut(s) 5, 103, 309
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 124
BseDI CCNNGG 3 cut(s) 5, 103, 309
BseGI GGATG 3 cut(s) 99, 445, 646
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 538, 650
BseXI GCAGC 5 cut(s) 146, 168, 279, 379, 410
BseYI CCCAGC 1 cut(s) 277
BsgI GTGCAG 3 cut(s) 59, 287, 580
Bsh1236I CGCG 2 cut(s) 7, 530
BshFI GGCC 3 cut(s) 10, 308, 394
BshNI GGYRCC 1 cut(s) 86
BsiSI CCGG 2 cut(s) 84, 309
BslFI GGGAC 3 cut(s) 254, 299, 490
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 3 cut(s) 254, 299, 490
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 3 cut(s) 10, 308, 394
Bso31I GGTCTC 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 52
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 1 cut(s) 579
BspACI CCGC 8 cut(s) 5, 7, 16, 37, 79, 283, 322, 614
BspANI GGCC 3 cut(s) 10, 308, 394
BspCNI CTCAG 1 cut(s) 249
BspFNI CGCG 2 cut(s) 7, 530
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 1 cut(s) 574
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 123
BssECI CCNNGG 3 cut(s) 5, 103, 309
BssMI GATC 1 cut(s) 579
Bst6I CTCTTC 2 cut(s) 405, 515
BstAPI GCANNNNNTGC 1 cut(s) 274
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 7 cut(s) 48, 185, 275, 306, 320, 359, 543
BstDEI CTNAG 1 cut(s) 236
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 3 cut(s) 99, 445, 646
BstFNI CGCG 2 cut(s) 7, 530
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 1 cut(s) 582
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 1 cut(s) 579
BstMWI GCNNNNNNNGC 6 cut(s) 114, 274, 289, 301, 367, 620
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 308
BstUI CGCG 2 cut(s) 7, 530
BstV1I GCAGC 5 cut(s) 146, 168, 279, 379, 410
BstV2I GAAGAC 2 cut(s) 369, 657
BsuRI GGCC 3 cut(s) 10, 308, 394
BtgI CCRYGG 1 cut(s) 5
BtrI CACGTC 1 cut(s) 480
BtsCI GGATG 3 cut(s) 99, 445, 646
BtsI GCAGTG 4 cut(s) 126, 263, 275, 332
BtsIMutI CAGTG 4 cut(s) 126, 263, 275, 332
Cac8I GCNNGC 7 cut(s) 48, 185, 275, 306, 320, 359, 543
CfoI GCGC 1 cut(s) 530
Cfr42I CCGCGG 1 cut(s) 8
Csp6I GTAC 4 cut(s) 124, 417, 601, 627
CviAII CATG 3 cut(s) 180, 274, 497
CviQI GTAC 4 cut(s) 124, 417, 601, 627
DdeI CTNAG 1 cut(s) 236
DpnI GATC 1 cut(s) 581
DpnII GATC 1 cut(s) 579
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 2 cut(s) 405, 515
EarI CTCTTC 2 cut(s) 405, 515
Eco24I GRGCYC 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 52
FaeI CATG 3 cut(s) 183, 277, 500
FaiI YATR 5 cut(s) 181, 275, 345, 498, 660
FaqI GGGAC 3 cut(s) 254, 299, 490
FatI CATG 3 cut(s) 179, 273, 496
FauI CCCGC 1 cut(s) 276
FokI GGATG 3 cut(s) 86, 452, 653
FriOI GRGCYC 1 cut(s) 52
FspBI CTAG 2 cut(s) 12, 624
GlaI GCGC 1 cut(s) 529
GsaI CCCAGC 1 cut(s) 281
HaeIII GGCC 3 cut(s) 10, 308, 394
HapII CCGG 2 cut(s) 84, 309
HhaI GCGC 1 cut(s) 530
Hin1II CATG 3 cut(s) 183, 277, 500
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HinfI GANTC 1 cut(s) 299
HpaII CCGG 2 cut(s) 84, 309
HphI GGTGA 2 cut(s) 130, 415
Hpy166II GTNNAC 2 cut(s) 124, 126
Hpy188III TCNNGA 1 cut(s) 511
Hpy8I GTNNAC 2 cut(s) 124, 126
Hpy99I CGWCG 2 cut(s) 388, 391
HpyAV CCTTC 2 cut(s) 37, 103
HpyCH4IV ACGT 2 cut(s) 248, 479
HpyCH4V TGCA 6 cut(s) 76, 156, 183, 268, 273, 561
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 274, 289, 301, 367, 620
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 2 cut(s) 248, 479
Hsp92II CATG 3 cut(s) 183, 277, 500
HspAI GCGC 1 cut(s) 528
KspI CCGCGG 1 cut(s) 8
Kzo9I GATC 1 cut(s) 579
LmnI GCTCC 4 cut(s) 43, 86, 525, 589
Lsp1109I GCAGC 5 cut(s) 146, 168, 279, 379, 410
MaeI CTAG 2 cut(s) 12, 624
MaeII ACGT 2 cut(s) 248, 479
MaeIII GTNAC 1 cut(s) 172
MalI GATC 1 cut(s) 581
MbiI CCGCTC 1 cut(s) 81
MboI GATC 1 cut(s) 579
MboII GAAGA 7 cut(s) 88, 369, 372, 422, 532, 547, 662
MhlI GDGCHC 1 cut(s) 52
MspA1I CMGCKG 3 cut(s) 7, 159, 340
MspI CCGG 2 cut(s) 84, 309
MspR9I CCNGG 1 cut(s) 310
Mva1269I GAATGC 1 cut(s) 509
MvnI CGCG 2 cut(s) 7, 530
MwoI GCNNNNNNNGC 6 cut(s) 114, 274, 289, 301, 367, 620
NciI CCSGG 1 cut(s) 310
NdeII GATC 1 cut(s) 579
NlaIII CATG 3 cut(s) 183, 277, 500
NlaIV GGNNCC 1 cut(s) 88
NmeAIII GCCGAG 1 cut(s) 84
NspI RCATGY 1 cut(s) 277
PaeI GCATGC 1 cut(s) 277
PctI GAATGC 1 cut(s) 509
PfeI GAWTC 1 cut(s) 299
PflMI CCANNNNNTGG 1 cut(s) 214
PspFI CCCAGC 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 88
PvuII CAGCTG 2 cut(s) 159, 340
RsaI GTAC 4 cut(s) 125, 418, 602, 628
RsaNI GTAC 4 cut(s) 124, 417, 601, 627
SacII CCGCGG 1 cut(s) 8
Sau3AI GATC 1 cut(s) 579
ScaI AGTACT 1 cut(s) 602
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 1 cut(s) 52
SetI ASST 7 cut(s) 95, 161, 251, 267, 342, 482, 547
Sfr303I CCGCGG 1 cut(s) 8
SgrBI CCGCGG 1 cut(s) 8
SphI GCATGC 1 cut(s) 277
SsiI CCGC 8 cut(s) 5, 7, 16, 37, 79, 283, 322, 614
SspMI CTAG 2 cut(s) 12, 624
StyD4I CCNGG 1 cut(s) 308
TaiI ACGT 2 cut(s) 251, 482
TaqI TCGA 2 cut(s) 389, 578
TatI WGTACW 2 cut(s) 123, 600
TauI GCSGC 5 cut(s) 7, 10, 18, 325, 617
TfiI GAWTC 1 cut(s) 299
TscAI CASTG 4 cut(s) 133, 263, 275, 332
TseI GCWGC 5 cut(s) 156, 159, 292, 367, 423
TspDTI ATGAA 2 cut(s) 88, 323
TspRI CASTG 4 cut(s) 133, 263, 275, 332
Van91I CCANNNNNTGG 1 cut(s) 214
XceI RCATGY 1 cut(s) 277
XspI CTAG 2 cut(s) 12, 624
ZrmI AGTACT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.