Rroxscaffold_5G00378490

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000995
Physical Location & Seq
Reverse (-)
58940556 .. 58945272
4717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00378490.1

Sequence Viewer

Length: 1941 bp
ATGAAATTCGAGAGGGAACCGGTGTTTGGCCAATTAAGTTTAAGCCACAAGCCTGATTATAAACCTTTTGATCACAATGAGATTACTCTGAATTCTGGGGGATTACAATCAGCAAATTGGATTGGGAAGGAAAGTCAGACCAGGGTTTTGTTTGATTCGAAGGACAATGAGGAAGATGCTGTTCAAAATGATGGAATTGACTTGACTGCATCAAGATTTGATTGTTCAAAGAGTGTGGATGATTTGGAAACCTGTAATGAAGGTGAAGTTAAGGGTTTTGTGCCTGAAAAATTAGAATCTCTAGGGAATCAATCAGAATATTATATGGACAAAAGTGTTACAGAATGCGACTTGCCAGAACTGACAGTTTGTTACAAAGAGAGTAGTTTCAATACTATCAAGGATATCTGCATAGATGAGGGAGTGCCTTCCCAAGAGAAGATCCCGTTTGAGACAGACGTGGATGAGAAGGCATTTTGCACTTATTTGTCTCCTGATAAGGATCAGAAACACCTACTTCTTGAACAACAAATGGAAATTTTTATGACCATTCCAGATGATTTCAAGTCTTCTGCACAGAAATATGATGTAAAGAAGGATTTTGTCATTGCTTGTGATGCCAAGGATCTGGTGCAGAGAGAAGAAGCAATGTATGATGCATCAGAGAAAACTGCAATTGATGTGTCCAGAGAAATGATTTCCCCTGAAAACTTGCGAACACAGAATTTGAACTCCAAGTCCTCTAGTAAGGCCAGCAATGAAGCTGTACAATATACTGTTCAGATATCGAGTGACAAGGAAAGTGAAATAGCAGAAACTCACAGCAGTGCTTTGGTTTCAGCAAAGGAAGAATCTAAGGATATTGAGAATGGAGCGTTCTTGAAGCGAGATCCTGTTCCTTCAGCTGTAGAACTAAACTGCCATGTTGATAAGTTATCTGACATTAGTGAAGTGGAGAATGGAAGCATTACTGTTGGTTTGGACCGTTCAGCACCTGTATCGATTAGTAGATTTCCAAAACTGGATCCTGTCCCTTCCACTGATGGATCAAACTGTCTTCTTGATGAGTTGGCCATCAATTTTAAAGCGGACAAAGGAGGAAGCAGTTCTTGTGGTTTGAACTGTTCAGACATTCTCGGGGATGTCTGTCCCCGAAATGGGTTCAGCAAACATATTGAAACTCAAAATATTTCTGAAGATGATAGTGATGACATATGTGATTCAAAAACTATTTCAAGCAAAGTTCAGCCTTGTTTAGCACCTGTGACTATTGCCAGAGGGGATTGTCCTGAAAATGGTGCCTGTCAACGTCCTGAAACTTCAAATACGTCCATGGTTGATGAGGACATTTCTGATTCAGAGATTGTTTCAAGCCAAGTTCAACATTCTTTAGTCCCTGCGACTATTGCCAGAGACGAATATCCTAAAGAGGGGAATTCTAATGCACAGGATGTTCCGATCCAAGTTCAACATGAGGTGGGAGAGACTAGTTTCTCTGCAGGAGTTCCTTTCTCGACCCTTATAAATTATAAGGAACCAATACCTTATTCAGGTACACACAATGCTTTGAGTGAAGTGCAACATGATCAGGGGGAATCAAGTTTCTCTGCAGTAGGTAACTCATTGAGTCTTATAAATAGTTCGGAACCAATACCTTATTCTGATTCAGATAGATTCCTTCTTCAACGTGATCTGGGAGATTCGAGTTTTGCTGCAGTAGGTCATGTCTCGAGTCTCATAAATAGTTCGGGACCAATACCTTATTCTGGCAGTATCTCCCTTCGATCAGACAGCAGTGCAACCAGCACCCGCTCATTTGCCTTCCCTGTTATGCATCCGCATGAACCATTCAGCAGTCCAGTGAGAATGGCAAAAGCTGACCGCAAGCTTTTGCGTAAACATAGGGGTTGGAAGCGGGGTATTTTCTGCTGTAGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

646

Amino Acids

70.9

Weight (kDa)

4.63

Isoelectric Point (pI)

51.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 60, 1523, 1530, 1634
AccB1I GGYRCC 1 cut(s) 1298
AccBSI CCGCTC 1 cut(s) 1812
AciI CCGC 5 cut(s) 1088, 1810, 1838, 1882, 1915
AclWI GGATC 8 cut(s) 436, 510, 633, 884, 1019, 1032, 1054, 1453
AcoI YGGCCR 2 cut(s) 28, 1071
AcsI RAATTY 5 cut(s) 5, 91, 537, 724, 1435
AcuI CTGAAG 2 cut(s) 885, 1215
AfaI GTAC 2 cut(s) 768, 1555
AfiI CCNNNNNNNGG 7 cut(s) 26, 1157, 1158, 1295, 1430, 1550, 1766
AgeI ACCGGT 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 1487
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 1 cut(s) 140
AjuI GAANNNNNNNTTGG 8 cut(s) 9, 41, 1531, 1563, 1642, 1674, 1747, 1779
AleI CACNNNNGTG 1 cut(s) 825
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
AluBI AGCT 4 cut(s) 764, 905, 1877, 1888
AluI AGCT 4 cut(s) 764, 905, 1877, 1888
Alw26I GTCTC 6 cut(s) 446, 495, 1407, 1478, 1732, 1739
AlwI GGATC 8 cut(s) 436, 510, 633, 884, 1019, 1032, 1054, 1453
AlwNI CAGNNNCTG 1 cut(s) 995
Ama87I CYCGRG 2 cut(s) 1136, 1729
AoxI GGCC 3 cut(s) 28, 750, 1071
ApeKI GCWGC 1 cut(s) 1712
ApoI RAATTY 5 cut(s) 5, 91, 537, 724, 1435
ArsI GACNNNNNNTTYG 2 cut(s) 130, 162
AsiGI ACCGGT 1 cut(s) 19
Asp700I GAANNNNTTC 1 cut(s) 1105
AspS9I GGNCC 2 cut(s) 982, 1751
AsuHPI GGTGA 1 cut(s) 275
AsuII TTCGAA 1 cut(s) 158
AvaI CYCGRG 2 cut(s) 1136, 1729
AvaII GGWCC 2 cut(s) 982, 1751
BalI TGGCCA 2 cut(s) 30, 1073
BamHI GGATCC 1 cut(s) 1024
BanI GGYRCC 1 cut(s) 1298
BbsI GAAGAC 2 cut(s) 561, 1049
BbvI GCAGC 1 cut(s) 1699
BccI CCATC 3 cut(s) 185, 1037, 1082
BcgI CGANNNNNNTGC 2 cut(s) 981, 1015
BciT130I CCWGG 1 cut(s) 142
BclI TGATCA 2 cut(s) 70, 1585
BcoDI GTCTC 6 cut(s) 446, 495, 1407, 1478, 1732, 1739
BcuI ACTAGT 1 cut(s) 1487
BfaI CTAG 3 cut(s) 302, 744, 1488
BfmI CTRYAG 5 cut(s) 906, 1497, 1608, 1713, 1930
BisI GCNGC 1 cut(s) 1713
BlsI GCNGC 1 cut(s) 1714
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 2 cut(s) 982, 1751
BmeT110I CYCGRG 2 cut(s) 1136, 1729
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 982, 1751
BmiI GGNNCC 6 cut(s) 18, 1026, 1300, 1536, 1647, 1752
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 6 cut(s) 166, 218, 607, 646, 668, 1843
BpiI GAAGAC 2 cut(s) 561, 1049
Bpu14I TTCGAA 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 1001
BsaBI GATNNNNATC 2 cut(s) 106, 501
BsaJI CCNNGG 3 cut(s) 141, 621, 1332
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 1157, 1158, 1295, 1430, 1550, 1766
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 1026, 1859
Bse3DI GCAATG 3 cut(s) 606, 654, 763
Bse8I GATNNNNATC 2 cut(s) 106, 501
BseBI CCWGG 1 cut(s) 142
BseCI ATCGAT 1 cut(s) 1001
BseDI CCNNGG 3 cut(s) 141, 621, 1332
BseGI GGATG 5 cut(s) 244, 469, 1147, 1456, 1834
BseJI GATNNNNATC 2 cut(s) 106, 501
BseLI CCNNNNNNNGG 7 cut(s) 26, 1157, 1158, 1295, 1430, 1550, 1766
BseMI GCAATG 3 cut(s) 606, 654, 763
BseNI ACTGG 2 cut(s) 1026, 1859
BseXI GCAGC 1 cut(s) 1699
BsgI GTGCAG 2 cut(s) 558, 653
BshFI GGCC 3 cut(s) 30, 752, 1073
BshNI GGYRCC 1 cut(s) 1298
BshTI ACCGGT 1 cut(s) 19
BshVI ATCGAT 1 cut(s) 1001
BsiHKCI CYCGRG 2 cut(s) 1136, 1729
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 4 cut(s) 1016, 1134, 1379, 1764
BslI CCNNNNNNNGG 7 cut(s) 26, 1157, 1158, 1295, 1430, 1550, 1766
BsmAI GTCTC 6 cut(s) 446, 495, 1407, 1478, 1732, 1739
BsmBI CGTCTC 1 cut(s) 1407
BsmFI GGGAC 4 cut(s) 1016, 1134, 1379, 1764
BsmI GAATGC 1 cut(s) 350
BsnI GGCC 3 cut(s) 30, 752, 1073
BsoBI CYCGRG 2 cut(s) 1136, 1729
Bsp119I TTCGAA 1 cut(s) 158
Bsp1407I TGTACA 1 cut(s) 766
Bsp19I CCATGG 1 cut(s) 1332
BspACI CCGC 5 cut(s) 1088, 1810, 1838, 1882, 1915
BspANI GGCC 3 cut(s) 30, 752, 1073
BspDI ATCGAT 1 cut(s) 1001
BspLI GGNNCC 6 cut(s) 18, 1026, 1300, 1536, 1647, 1752
BspMAI CTGCAG 3 cut(s) 1501, 1612, 1717
BspPI GGATC 8 cut(s) 436, 510, 633, 884, 1019, 1032, 1054, 1453
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 1298
BsrBI CCGCTC 1 cut(s) 1812
BsrDI GCAATG 3 cut(s) 606, 654, 763
BsrFI RCCGGY 1 cut(s) 19
BsrGI TGTACA 1 cut(s) 766
BsrI ACTGG 2 cut(s) 1026, 1859
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 3 cut(s) 141, 621, 1332
BssT1I CCWWGG 2 cut(s) 621, 1332
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 6 cut(s) 367, 778, 973, 986, 1055, 1124
BstAUI TGTACA 1 cut(s) 766
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 754, 1886
BstDEI CTNAG 1 cut(s) 855
BstDSI CCRYGG 1 cut(s) 1332
BstENI CCTNNNNNAGG 1 cut(s) 1548
BstF5I GGATG 5 cut(s) 244, 469, 1147, 1456, 1834
BstMAI GTCTC 6 cut(s) 446, 495, 1407, 1478, 1732, 1739
BstMWI GCNNNNNNNGC 2 cut(s) 617, 1406
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 5 cut(s) 906, 1497, 1608, 1713, 1930
BstV1I GCAGC 1 cut(s) 1699
BstV2I GAAGAC 2 cut(s) 561, 1049
BstX2I RGATCY 4 cut(s) 441, 625, 889, 1024
BstXI CCANNNNNNTGG 1 cut(s) 628
BstYI RGATCY 4 cut(s) 441, 625, 889, 1024
Bsu15I ATCGAT 1 cut(s) 1001
BsuRI GGCC 3 cut(s) 30, 752, 1073
BsuTUI ATCGAT 1 cut(s) 1001
BtgI CCRYGG 1 cut(s) 1332
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 5 cut(s) 244, 469, 1147, 1456, 1834
BtsI GCAGTG 2 cut(s) 832, 1801
BtsIMutI CAGTG 4 cut(s) 832, 1038, 1801, 1866
Cac8I GCNNGC 2 cut(s) 754, 1886
CaiI CAGNNNCTG 1 cut(s) 995
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 982, 1751
ClaI ATCGAT 1 cut(s) 1001
Csp6I GTAC 2 cut(s) 767, 1554
CspAI ACCGGT 1 cut(s) 19
CspCI CAANNNNNGTGG 2 cut(s) 216, 251
CviAII CATG 6 cut(s) 923, 1333, 1472, 1583, 1724, 1841
CviQI GTAC 2 cut(s) 767, 1554
DdeI CTNAG 1 cut(s) 855
DraI TTTAAA 1 cut(s) 1084
EaeI YGGCCR 2 cut(s) 28, 1071
Eco130I CCWWGG 2 cut(s) 621, 1332
Eco32I GATATC 2 cut(s) 406, 786
Eco47I GGWCC 2 cut(s) 982, 1751
Eco57I CTGAAG 2 cut(s) 885, 1215
Eco88I CYCGRG 2 cut(s) 1136, 1729
EcoNI CCTNNNNNAGG 1 cut(s) 1548
EcoRI GAATTC 2 cut(s) 91, 1435
EcoRII CCWGG 1 cut(s) 140
EcoRV GATATC 2 cut(s) 406, 786
EcoT14I CCWWGG 2 cut(s) 621, 1332
EcoT22I ATGCAT 2 cut(s) 661, 1836
ErhI CCWWGG 2 cut(s) 621, 1332
Esp3I CGTCTC 1 cut(s) 1407
FaeI CATG 6 cut(s) 926, 1336, 1475, 1586, 1727, 1844
FalI AAGNNNNNCTT 2 cut(s) 1093, 1125
FaqI GGGAC 4 cut(s) 1016, 1134, 1379, 1764
FatI CATG 6 cut(s) 922, 1332, 1471, 1582, 1723, 1840
FauI CCCGC 2 cut(s) 1817, 1908
FauNDI CATATG 1 cut(s) 1214
FbaI TGATCA 2 cut(s) 70, 1585
Fnu4HI GCNGC 1 cut(s) 1713
FokI GGATG 5 cut(s) 251, 476, 1154, 1463, 1821
Fsp4HI GCNGC 1 cut(s) 1713
FspBI CTAG 3 cut(s) 302, 744, 1488
GluI GCNGC 1 cut(s) 1713
HaeIII GGCC 3 cut(s) 30, 752, 1073
HapII CCGG 1 cut(s) 20
Hin1II CATG 6 cut(s) 926, 1336, 1475, 1586, 1727, 1844
HincII GTYRAC 1 cut(s) 1307
HindII GTYRAC 1 cut(s) 1307
HindIII AAGCTT 1 cut(s) 1886
HpaII CCGG 1 cut(s) 20
HphI GGTGA 1 cut(s) 275
Hpy166II GTNNAC 3 cut(s) 1307, 1556, 1898
Hpy8I GTNNAC 3 cut(s) 1307, 1556, 1898
HpyCH4III ACNGT 6 cut(s) 367, 778, 973, 986, 1055, 1124
HpyCH4IV ACGT 4 cut(s) 459, 1309, 1328, 1687
HpyF10VI GCNNNNNNNGC 2 cut(s) 617, 1406
HpyF3I CTNAG 1 cut(s) 855
HpySE526I ACGT 4 cut(s) 459, 1309, 1328, 1687
Hsp92II CATG 6 cut(s) 926, 1336, 1475, 1586, 1727, 1844
Ksp22I TGATCA 2 cut(s) 70, 1585
LmnI GCTCC 1 cut(s) 872
Lsp1109I GCAGC 1 cut(s) 1699
LweI GCATC 6 cut(s) 166, 218, 607, 646, 668, 1843
MaeI CTAG 3 cut(s) 302, 744, 1488
MaeII ACGT 4 cut(s) 459, 1309, 1328, 1687
MaeIII GTNAC 5 cut(s) 337, 371, 791, 1264, 1616
MbiI CCGCTC 1 cut(s) 1812
MboII GAAGA 8 cut(s) 185, 451, 561, 653, 860, 1049, 1208, 1673
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 4 cut(s) 441, 625, 889, 1024
MlsI TGGCCA 2 cut(s) 30, 1073
MluNI TGGCCA 2 cut(s) 30, 1073
MlyI GAGTC 2 cut(s) 1636, 1741
MmeI TCCRAC 1 cut(s) 1889
MnlI CCTC 9 cut(s) 6, 163, 412, 751, 1091, 1271, 1336, 1423, 1468
Mox20I TGGCCA 2 cut(s) 30, 1073
Mph1103I ATGCAT 2 cut(s) 661, 1836
MroXI GAANNNNTTC 1 cut(s) 1105
MscI TGGCCA 2 cut(s) 30, 1073
MseI TTAA 4 cut(s) 35, 41, 270, 1083
MslI CAYNNNNRTG 2 cut(s) 825, 1839
Msp20I TGGCCA 2 cut(s) 30, 1073
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 142
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 350
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 617, 1406
NcoI CCATGG 1 cut(s) 1332
NdeI CATATG 1 cut(s) 1214
NlaIII CATG 6 cut(s) 926, 1336, 1475, 1586, 1727, 1844
NlaIV GGNNCC 6 cut(s) 18, 1026, 1300, 1536, 1647, 1752
NmuCI GTSAC 2 cut(s) 791, 1264
NsiI ATGCAT 2 cut(s) 661, 1836
NspV TTCGAA 1 cut(s) 158
OliI CACNNNNGTG 1 cut(s) 825
PaeR7I CTCGAG 1 cut(s) 1729
PctI GAATGC 1 cut(s) 350
PdmI GAANNNNTTC 1 cut(s) 1105
PinAI ACCGGT 1 cut(s) 19
PkrI GCNGC 1 cut(s) 1714
PleI GAGTC 2 cut(s) 1635, 1740
PpsI GAGTC 2 cut(s) 1635, 1740
PsiI TTATAA 4 cut(s) 60, 1523, 1530, 1634
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 6 cut(s) 18, 1026, 1300, 1536, 1647, 1752
PspPI GGNCC 2 cut(s) 982, 1751
PstI CTGCAG 3 cut(s) 1501, 1612, 1717
PstNI CAGNNNCTG 1 cut(s) 995
PsuI RGATCY 4 cut(s) 441, 625, 889, 1024
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 2 cut(s) 768, 1555
RsaNI GTAC 2 cut(s) 767, 1554
RseI CAYNNNNRTG 2 cut(s) 825, 1839
SaqAI TTAA 4 cut(s) 35, 41, 270, 1083
SatI GCNGC 1 cut(s) 1713
Sau96I GGNCC 2 cut(s) 982, 1751
SchI GAGTC 2 cut(s) 1636, 1741
ScrFI CCNGG 1 cut(s) 142
SfaNI GCATC 6 cut(s) 166, 218, 607, 646, 668, 1843
SfcI CTRYAG 5 cut(s) 906, 1497, 1608, 1713, 1930
Sfr274I CTCGAG 1 cut(s) 1729
SfuI TTCGAA 1 cut(s) 158
SinI GGWCC 2 cut(s) 982, 1751
SlaI CTCGAG 1 cut(s) 1729
SmiMI CAYNNNNRTG 2 cut(s) 825, 1839
SmlI CTYRAG 1 cut(s) 1729
SmoI CTYRAG 1 cut(s) 1729
SpeI ACTAGT 1 cut(s) 1487
SsiI CCGC 5 cut(s) 1088, 1810, 1838, 1882, 1915
SspI AATATT 2 cut(s) 320, 1189
SspMI CTAG 3 cut(s) 302, 744, 1488
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 2 cut(s) 621, 1332
TaaI ACNGT 6 cut(s) 367, 778, 973, 986, 1055, 1124
TaiI ACGT 4 cut(s) 462, 1312, 1331, 1690
TaqI TCGA 8 cut(s) 9, 158, 788, 1001, 1514, 1703, 1730, 1783
TatI WGTACW 1 cut(s) 766
Tru1I TTAA 4 cut(s) 35, 41, 270, 1083
Tru9I TTAA 4 cut(s) 35, 41, 270, 1083
TscAI CASTG 4 cut(s) 832, 1045, 1801, 1866
TseFI GTSAC 2 cut(s) 791, 1264
TseI GCWGC 1 cut(s) 1712
Tsp45I GTSAC 2 cut(s) 791, 1264
TspDTI ATGAA 4 cut(s) 17, 273, 774, 1857
TspRI CASTG 4 cut(s) 832, 1045, 1801, 1866
VpaK11BI GGWCC 2 cut(s) 982, 1751
XagI CCTNNNNNAGG 1 cut(s) 1548
XapI RAATTY 5 cut(s) 5, 91, 537, 724, 1435
XhoI CTCGAG 1 cut(s) 1729
XmnI GAANNNNTTC 1 cut(s) 1105
XspI CTAG 3 cut(s) 302, 744, 1488
Zsp2I ATGCAT 2 cut(s) 661, 1836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.