pycom14g17950

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000995
Physical Location & Seq
Forward (+)
19739687 .. 19741124
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g17950.2

Sequence Viewer

Length: 909 bp
ATGGATGGCCATTCAACCGCGGTCTTCCAGCCTGTTTACAAGTCCTTCAAGTTCAAAAGTTCCGAAGCCACGAAGGGATTGAAGGTGGAGAAAAGTACTAGTTCGAACGATGATGATAATCTGTTCATTTCCGATTTGCTAAGTCAAGACGGTGAGGCGGTTCTAAAACCACATCCAGATGACAGTCGGAATATGCACGCTACAAGGGACGCCGTGACAGCTAGTGATTGCGCGGCTTGGACGAATTCTACTAGAACGGCTGCTATGGACTATGACTTGCCAGAGCTGGTTGCATTCCTTCAAGGGAGCAGCAACTACCAGTTCTTTAAGGACATTGGCATTGACAAGGAAGTTCGATGCGAGGACAAGTGTTTCGTAGAGCACTGTGAATCCTATCATAGTAGCTTTTCTTGCGTACTTGATTCTGATCCGGACAGCGAGGGCGAGTCAATTAGAGAATCTCTGGACTCGGAGTCATCCACTTCAACCGATGAGAACGACGGCAAAAAGGATGATCGATCAAATGATCATTCAATCAAGAAGGGTACAAGTAACGCAGCCTTGGCTGCAAACTCAGTAGGACTATCAATAACTAAGGCAATTGGCGACAACAGCCGGACAAGCAATGCCTCTTCAAAAGGTGACCTAGAAAATACAATCGGAGCGAAGTTTCATGCCCCAGAAACGGAAAACTTGTTGAGGCATGATTCGTCGAAAGATAGGATGTCAGATGGCCTAGCAGAATCGTGTCAACGACACCAGCATTGCCGCGGAGAATCCAGTTCCTCTGCATCCGAACTTCCTTCGGGCTCATTTTCTTACTTGGGAGGAATACCGGGTTTTGGCAGCATCTCTCATCGGTCGAGCAGCAGCACAACTAGCTCCGAGTCTTTCGCTTTTCCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

303

Amino Acids

32.6

Weight (kDa)

4.9

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 20, 233, 771
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 6 cut(s) 18, 20, 158, 233, 769, 771
AclWI GGATC 1 cut(s) 422
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 3 cut(s) 97, 417, 547
AfiI CCNNNNNNNGG 2 cut(s) 685, 842
AgsI TTSAA 8 cut(s) 15, 49, 55, 82, 302, 486, 534, 636
AhdI GACNNNNNGTC 1 cut(s) 472
AhlI ACTAGT 1 cut(s) 98
AluBI AGCT 4 cut(s) 221, 286, 405, 882
AluI AGCT 4 cut(s) 221, 286, 405, 882
Alw21I GWGCWC 1 cut(s) 384
AlwI GGATC 1 cut(s) 422
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 2 cut(s) 7, 733
ApeKI GCWGC 7 cut(s) 260, 309, 557, 566, 846, 867, 870
ApoI RAATTY 1 cut(s) 244
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AspLEI GCGC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 837
AsuHPI GGTGA 2 cut(s) 164, 653
AsuII TTCGAA 1 cut(s) 104
BalI TGGCCA 1 cut(s) 9
BanII GRGCYC 1 cut(s) 812
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 7 cut(s) 247, 321, 553, 569, 858, 879, 882
BccI CCATC 1 cut(s) 725
BceAI ACGGC 3 cut(s) 197, 273, 517
BclI TGATCA 1 cut(s) 526
BcnI CCSGG 1 cut(s) 837
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 6 cut(s) 99, 222, 252, 647, 737, 879
BisI GCNGC 9 cut(s) 234, 261, 310, 558, 567, 769, 847, 868, 871
BlsI GCNGC 9 cut(s) 235, 262, 311, 559, 568, 770, 848, 869, 872
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 837
BmeRI GACNNNNNGTC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 837
BmsI GCATC 3 cut(s) 347, 800, 858
BpiI GAAGAC 1 cut(s) 16
Bpu14I TTCGAA 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 837
Bsa29I ATCGAT 1 cut(s) 517
BsaBI GATNNNNATC 2 cut(s) 117, 426
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 18, 561, 769
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 2 cut(s) 685, 842
Bse1I ACTGG 2 cut(s) 319, 780
Bse3DI GCAATG 2 cut(s) 631, 763
Bse8I GATNNNNATC 2 cut(s) 117, 426
BseAI TCCGGA 1 cut(s) 430
BseCI ATCGAT 1 cut(s) 517
BseDI CCNNGG 3 cut(s) 18, 561, 769
BseGI GGATG 6 cut(s) 10, 172, 476, 517, 729, 791
BseJI GATNNNNATC 2 cut(s) 117, 426
BseLI CCNNNNNNNGG 2 cut(s) 685, 842
BseMI GCAATG 2 cut(s) 631, 763
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 2 cut(s) 319, 780
BseXI GCAGC 7 cut(s) 247, 321, 553, 569, 858, 879, 882
Bsh1236I CGCG 3 cut(s) 20, 233, 771
Bsh1285I CGRYCG 1 cut(s) 863
BshFI GGCC 2 cut(s) 9, 735
BshVI ATCGAT 1 cut(s) 517
BsiEI CGRYCG 1 cut(s) 863
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 3 cut(s) 431, 616, 836
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 685, 842
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 2 cut(s) 9, 735
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 384, 812
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 4 cut(s) 427, 514, 518, 526
BspACI CCGC 6 cut(s) 18, 20, 158, 233, 769, 771
BspANI GGCC 2 cut(s) 9, 735
BspCNI CTCAG 1 cut(s) 587
BspDI ATCGAT 1 cut(s) 517
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 3 cut(s) 20, 233, 771
BspPI GGATC 1 cut(s) 422
BspT104I TTCGAA 1 cut(s) 104
BsrDI GCAATG 2 cut(s) 631, 763
BsrI ACTGG 2 cut(s) 319, 780
BssECI CCNNGG 3 cut(s) 18, 561, 769
BssMI GATC 4 cut(s) 427, 514, 518, 526
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 561
Bst4CI ACNGT 3 cut(s) 152, 185, 386
Bst6I CTCTTC 1 cut(s) 637
BstACI GRCGYC 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 140, 574, 594
BstDSI CCRYGG 2 cut(s) 18, 769
BstEII GGTNACC 1 cut(s) 641
BstF5I GGATG 6 cut(s) 10, 172, 476, 517, 729, 791
BstFNI CGCG 3 cut(s) 20, 233, 771
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 4 cut(s) 430, 517, 521, 529
BstMBI GATC 4 cut(s) 427, 514, 518, 526
BstMCI CGRYCG 1 cut(s) 863
BstMWI GCNNNNNNNGC 7 cut(s) 218, 411, 563, 566, 612, 621, 879
BstPI GGTNACC 1 cut(s) 641
BstSCI CCNGG 1 cut(s) 835
BstUI CGCG 3 cut(s) 20, 233, 771
BstV1I GCAGC 7 cut(s) 247, 321, 553, 569, 858, 879, 882
BstV2I GAAGAC 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 517
BsuRI GGCC 2 cut(s) 9, 735
BsuTUI ATCGAT 1 cut(s) 517
BtgI CCRYGG 2 cut(s) 18, 769
BtsCI GGATG 6 cut(s) 10, 172, 476, 517, 729, 791
BtsIMutI CAGTG 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 198
CfoI GCGC 1 cut(s) 233
Cfr42I CCGCGG 2 cut(s) 21, 772
ClaI ATCGAT 1 cut(s) 517
CseI GACGC 1 cut(s) 218
Csp6I GTAC 3 cut(s) 96, 416, 546
CviAII CATG 3 cut(s) 674, 704, 904
CviQI GTAC 3 cut(s) 96, 416, 546
DdeI CTNAG 3 cut(s) 140, 574, 594
DpnI GATC 4 cut(s) 429, 516, 520, 528
DpnII GATC 4 cut(s) 427, 514, 518, 526
DriI GACNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 637
Eam1105I GACNNNNNGTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 637
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 812
Eco91I GGTNACC 1 cut(s) 641
EcoO65I GGTNACC 1 cut(s) 641
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 812
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 3 cut(s) 677, 707, 907
FaiI YATR 7 cut(s) 194, 266, 273, 399, 675, 705, 905
FaqI GGGAC 1 cut(s) 221
FatI CATG 3 cut(s) 673, 703, 903
FbaI TGATCA 1 cut(s) 526
Fnu4HI GCNGC 9 cut(s) 234, 261, 310, 558, 567, 769, 847, 868, 871
FokI GGATG 6 cut(s) 17, 159, 463, 524, 736, 778
FriOI GRGCYC 1 cut(s) 812
Fsp4HI GCNGC 9 cut(s) 234, 261, 310, 558, 567, 769, 847, 868, 871
FspBI CTAG 6 cut(s) 99, 222, 252, 647, 737, 879
GlaI GCGC 1 cut(s) 232
GluI GCNGC 9 cut(s) 234, 261, 310, 558, 567, 769, 847, 868, 871
HaeIII GGCC 2 cut(s) 9, 735
HapII CCGG 3 cut(s) 431, 616, 836
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 3 cut(s) 677, 707, 907
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 1 cut(s) 752
HindII GTYRAC 1 cut(s) 752
HpaII CCGG 3 cut(s) 431, 616, 836
HphI GGTGA 2 cut(s) 164, 653
Hpy166II GTNNAC 2 cut(s) 37, 752
Hpy188I TCNGA 9 cut(s) 64, 133, 189, 427, 472, 662, 730, 796, 886
Hpy188III TCNNGA 5 cut(s) 146, 176, 431, 464, 538
Hpy8I GTNNAC 2 cut(s) 37, 752
Hpy99I CGWCG 2 cut(s) 503, 715
HpyAV CCTTC 6 cut(s) 55, 67, 76, 308, 535, 813
HpyCH4III ACNGT 3 cut(s) 152, 185, 386
HpyCH4V TGCA 4 cut(s) 196, 293, 569, 791
HpyF10VI GCNNNNNNNGC 7 cut(s) 218, 411, 563, 566, 612, 621, 879
HpyF3I CTNAG 3 cut(s) 140, 574, 594
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 3 cut(s) 677, 707, 907
HspAI GCGC 1 cut(s) 231
Kpn2I TCCGGA 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 526
KspI CCGCGG 2 cut(s) 21, 772
Kzo9I GATC 4 cut(s) 427, 514, 518, 526
LmnI GCTCC 3 cut(s) 306, 662, 887
Lsp1109I GCAGC 7 cut(s) 247, 321, 553, 569, 858, 879, 882
LweI GCATC 3 cut(s) 347, 800, 858
MaeI CTAG 6 cut(s) 99, 222, 252, 647, 737, 879
MaeIII GTNAC 3 cut(s) 214, 551, 641
MalI GATC 4 cut(s) 429, 516, 520, 528
MboI GATC 4 cut(s) 427, 514, 518, 526
MboII GAAGA 2 cut(s) 16, 624
MfeI CAATTG 1 cut(s) 600
MhlI GDGCHC 2 cut(s) 384, 812
MlsI TGGCCA 1 cut(s) 9
MluCI AATT 3 cut(s) 244, 450, 600
MluNI TGGCCA 1 cut(s) 9
MlyI GAGTC 4 cut(s) 455, 461, 482, 896
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 7 cut(s) 148, 355, 433, 640, 693, 796, 821
Mox20I TGGCCA 1 cut(s) 9
MroI TCCGGA 1 cut(s) 430
MscI TGGCCA 1 cut(s) 9
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 177
Msp20I TGGCCA 1 cut(s) 9
MspA1I CMGCKG 2 cut(s) 20, 771
MspI CCGG 3 cut(s) 431, 616, 836
MspR9I CCNGG 1 cut(s) 837
MunI CAATTG 1 cut(s) 600
Mva1269I GAATGC 1 cut(s) 293
MvnI CGCG 3 cut(s) 20, 233, 771
MwoI GCNNNNNNNGC 7 cut(s) 218, 411, 563, 566, 612, 621, 879
NciI CCSGG 1 cut(s) 837
NdeII GATC 4 cut(s) 427, 514, 518, 526
NlaIII CATG 3 cut(s) 677, 707, 907
NmuCI GTSAC 2 cut(s) 214, 641
NspV TTCGAA 1 cut(s) 104
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 6 cut(s) 389, 422, 458, 707, 743, 776
PkrI GCNGC 9 cut(s) 235, 262, 311, 559, 568, 770, 848, 869, 872
PleI GAGTC 4 cut(s) 454, 461, 481, 895
PpsI GAGTC 4 cut(s) 454, 461, 481, 895
PspEI GGTNACC 1 cut(s) 641
RsaI GTAC 3 cut(s) 97, 417, 547
RsaNI GTAC 3 cut(s) 96, 416, 546
RseI CAYNNNNRTG 1 cut(s) 177
SacII CCGCGG 2 cut(s) 21, 772
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 9 cut(s) 234, 261, 310, 558, 567, 769, 847, 868, 871
Sau3AI GATC 4 cut(s) 427, 514, 518, 526
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 4 cut(s) 455, 461, 482, 896
ScrFI CCNGG 1 cut(s) 837
SduI GDGCHC 2 cut(s) 384, 812
SetI ASST 7 cut(s) 87, 223, 288, 407, 643, 648, 884
SfaNI GCATC 3 cut(s) 347, 800, 858
Sfr303I CCGCGG 2 cut(s) 21, 772
SfuI TTCGAA 1 cut(s) 104
SgrBI CCGCGG 2 cut(s) 21, 772
SmiMI CAYNNNNRTG 1 cut(s) 177
SpeI ACTAGT 1 cut(s) 98
Sse9I AATT 3 cut(s) 244, 450, 600
SsiI CCGC 6 cut(s) 18, 20, 158, 233, 769, 771
SspMI CTAG 6 cut(s) 99, 222, 252, 647, 737, 879
StyD4I CCNGG 1 cut(s) 835
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 3 cut(s) 152, 185, 386
TaqI TCGA 5 cut(s) 104, 355, 517, 713, 863
TaqII GACCGA 1 cut(s) 849
TasI AATT 3 cut(s) 244, 450, 600
TatI WGTACW 1 cut(s) 95
TauI GCSGC 2 cut(s) 236, 771
TfiI GAWTC 6 cut(s) 389, 422, 458, 707, 743, 776
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 2 cut(s) 214, 641
TseI GCWGC 7 cut(s) 260, 309, 557, 566, 846, 867, 870
Tsp45I GTSAC 2 cut(s) 214, 641
TspDTI ATGAA 2 cut(s) 115, 662
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 1 cut(s) 389
XapI RAATTY 1 cut(s) 244
XspI CTAG 6 cut(s) 99, 222, 252, 647, 737, 879
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.