MD16G1055400.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000995
Physical Location & Seq
Reverse (-)
3898072 .. 3902822
4751 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1055400.v1.1.491

Sequence Viewer

Length: 1611 bp
ATGAAACTTGAGAGAGAACCAGTGTTTGGTCACTTACCTATAAGCCACATGCCTGATTCGAATCCTTTTAATTGTAATGACATTACTCTGGACTCCGCGGGATTACAGTCAACAAGTCAGAATAGGGAGAAGGAATCGGATTATTATATGGACAAAAGTGTTACGGAATGTGAACTGCCTGAATTGATAATTTGTTACAACGATAGCAGTTGTAATACAATTAAGGACATCTGTATTGATGAGGGATTGCCTTCCCAAGACAAGAATTGGGTTGAAACCGGGTTGGATGAGAAGGCATGCTGCACCTATTTACCTCCTGATGAGGATCAGAATAAGCAAATGCATGAAGAACAATTGGATATTGTTATTACCTTTCCAGATGATTTTAAATCTACTGCAAAAAATGATTTAGAGAAGGGGTTTTCCATTGCTTGCAATTCCAAGGAGCCAATGGAGAGAGGCGATGCTATAAATGATGGATCAGAGAGGACTGCAATTGACGTTTCCAAAGAAGATTCTTGCCCGGAAAATGTGCAAGGGTTTGGTGCAGGGAACCCTCACTCCAAGTCTTCTAATAAGGACAGCAATGAAGCCGAACAAGAGACTATTCAGATTTCAAGGGAAATAGCAAAGACTGAGAGCCCTGCTTTGGTTTCAGAAACTGAAGAGTCTAACCATATTGAGAAGGAAGCATTGTTTTCTGCAGCTGAAGAATCAAATTGCCTTCTCGGTGAGTTATCTGGCAATAGCAAGGCAGAGAGCGGAAACATTACTTCTGCTTTGGACGTTTCAGCACATGTATCCATTACCACAGATGTGTGTCCTCAAAATGGTGTATGCAATATGCCTATAGATGATGATAGTCATGATGAATGCCAATTTCATCATTGTCTAGCACCTGTGGTTACTGGAAGAGAAGAGTGTCCTGAGAATGGTGTACATCTCGATACTTCAAATACGTTCAACGTTGGTGGTGACATTTCTGATCCAATGATTGTTTCAAGCCAAGTTGAACATCGTTTAGCACCCGTGGTTACTGGAAGAGAGGAGTGTCCTGAGAATGGTGTCTGTCAACATCTTGATGCTTCAAACACGTCCAAGGTTGATGATGACATTTCTGATTCAAAGATTGTTTCGAGACAGGTTCAACATTATTTAGCTCCTTCGACTATTGCCAGAGATGAGTGTCCTCAGAATGGCGTCTGTCAAGGTCTCGAAACTACAAATACAACCAAGGTTAATGATGACACTTCTGATTCAAAGATTGTTTCAAGCCAGGTTCATCATTGGTTATCTCCTGAAACTTCATATATATCCATGGTTGAAGATGTGAATGCTGATGCACACAATGCTCCACTCCAATTTCAACGCAGTCTAGGAGAGTCAAGTTTCTCAGCAGCTGGTCACTTCTCGAGCCTCATAACCTCAGGTCCCTATTCCGGCAATGTCTCCCTCCGATCAGAAAGCAGCACCACCAGCACCCGCTCATTTGCCTTCCCTGTATTACAGTCCGAGTGGAACAGCAGTCCAGTTAGAATGGCGAAAGCTGACCGGAGTCATTTGCGGAAACATAGGGGTTGGAGGGGTTGTTTTCTTTGCTGTAGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

537

Amino Acids

58.72

Weight (kDa)

4.57

Isoelectric Point (pI)

58.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 26
AccBSI CCGCTC 2 cut(s) 762, 1485
AccII CGCG 1 cut(s) 98
AciI CCGC 5 cut(s) 96, 98, 762, 1483, 1564
AclI AACGTT 1 cut(s) 966
AclWI GGATC 3 cut(s) 333, 487, 980
AcuI CTGAAG 2 cut(s) 684, 729
AcyI GRCGYC 1 cut(s) 1200
AfaI GTAC 1 cut(s) 939
AfiI CCNNNNNNNGG 7 cut(s) 26, 649, 830, 932, 1061, 1196, 1439
AflIII ACRYGT 2 cut(s) 796, 1092
AjiI CACGTC 1 cut(s) 1095
AjnI CCWGG 1 cut(s) 1275
AjuI GAANNNNNNNTTGG 4 cut(s) 9, 41, 982, 1014
AleI CACNNNNGTG 1 cut(s) 815
AloI GAACNNNNNNTCC 2 cut(s) 545, 577
AluBI AGCT 5 cut(s) 707, 1160, 1400, 1547, 1605
AluI AGCT 5 cut(s) 707, 1160, 1400, 1547, 1605
Alw26I GTCTC 4 cut(s) 596, 1132, 1217, 1453
AlwI GGATC 3 cut(s) 333, 487, 980
AlwNI CAGNNNCTG 2 cut(s) 662, 1400
Ama87I CYCGRG 1 cut(s) 1411
ApeKI GCWGC 4 cut(s) 300, 704, 1397, 1467
AspS9I GGNCC 1 cut(s) 1430
AsuC2I CCSGG 2 cut(s) 280, 524
AsuHPI GGTGA 2 cut(s) 743, 986
AsuII TTCGAA 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 1411
AvaII GGWCC 1 cut(s) 1430
AxyI CCTNAGG 1 cut(s) 1426
BanII GRGCYC 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 4 cut(s) 287, 716, 1409, 1479
BccI CCATC 1 cut(s) 470
BciT130I CCWGG 1 cut(s) 1277
BciVI GTATCC 1 cut(s) 811
BcnI CCSGG 2 cut(s) 280, 524
BcoDI GTCTC 4 cut(s) 596, 1132, 1217, 1453
BfaI CTAG 2 cut(s) 893, 1376
BfmI CTRYAG 3 cut(s) 702, 849, 1600
BfuI GTATCC 1 cut(s) 811
BisI GCNGC 4 cut(s) 301, 705, 1398, 1468
BlsI GCNGC 4 cut(s) 302, 706, 1399, 1469
Bme1390I CCNGG 3 cut(s) 280, 524, 1277
Bme18I GGWCC 1 cut(s) 1430
BmeT110I CYCGRG 1 cut(s) 1411
BmgBI CACGTC 1 cut(s) 1095
BmgT120I GGNCC 1 cut(s) 1430
BmiI GGNNCC 3 cut(s) 447, 554, 1432
BmrFI CCNGG 3 cut(s) 280, 524, 1277
BmsI GCATC 3 cut(s) 454, 1072, 1330
BoxI GACNNNNGTC 1 cut(s) 1554
BpiI GAAGAC 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 280, 524
BsaBI GATNNNNATC 2 cut(s) 60, 324
BsaHI GRCGYC 1 cut(s) 1200
BsaI GGTCTC 1 cut(s) 1217
BsaJI CCNNGG 6 cut(s) 96, 441, 1029, 1098, 1233, 1317
BsaWI WCCGGW 1 cut(s) 1551
BsaXI ACNNNNNCTCC 4 cut(s) 545, 575, 1573, 1603
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 649, 830, 932, 1061, 1196, 1439
Bse1I ACTGG 4 cut(s) 20, 913, 1042, 1529
Bse21I CCTNAGG 1 cut(s) 1426
Bse3DI GCAATG 3 cut(s) 426, 592, 1450
Bse8I GATNNNNATC 2 cut(s) 60, 324
BseBI CCWGG 1 cut(s) 1277
BseDI CCNNGG 6 cut(s) 96, 441, 1029, 1098, 1233, 1317
BseGI GGATG 1 cut(s) 292
BseJI GATNNNNATC 2 cut(s) 60, 324
BseLI CCNNNNNNNGG 7 cut(s) 26, 649, 830, 932, 1061, 1196, 1439
BseMI GCAATG 3 cut(s) 426, 592, 1450
BseMII CTCAG 6 cut(s) 627, 918, 1047, 1205, 1407, 1440
BseNI ACTGG 4 cut(s) 20, 913, 1042, 1529
BseRI GAGGAG 1 cut(s) 1061
BseXI GCAGC 4 cut(s) 287, 716, 1409, 1479
BsgI GTGCAG 2 cut(s) 286, 567
Bsh1236I CGCG 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 1411
BsiSI CCGG 4 cut(s) 279, 524, 1440, 1552
BslFI GGGAC 1 cut(s) 1416
BslI CCNNNNNNNGG 7 cut(s) 26, 649, 830, 932, 1061, 1196, 1439
BsmAI GTCTC 4 cut(s) 596, 1132, 1217, 1453
BsmFI GGGAC 1 cut(s) 1416
BsmI GAATGC 2 cut(s) 878, 1339
Bso31I GGTCTC 1 cut(s) 1217
BsoBI CYCGRG 1 cut(s) 1411
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 644
Bsp1407I TGTACA 1 cut(s) 937
Bsp143I GATC 4 cut(s) 325, 479, 985, 1457
Bsp19I CCATGG 1 cut(s) 1317
BspACI CCGC 5 cut(s) 96, 98, 762, 1483, 1564
BspCNI CTCAG 6 cut(s) 628, 919, 1048, 1204, 1406, 1439
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 865
BspLI GGNNCC 3 cut(s) 447, 554, 1432
BspMAI CTGCAG 1 cut(s) 706
BspPI GGATC 3 cut(s) 333, 487, 980
BspT104I TTCGAA 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 1217
BsrBI CCGCTC 2 cut(s) 762, 1485
BsrDI GCAATG 3 cut(s) 426, 592, 1450
BsrGI TGTACA 1 cut(s) 937
BsrI ACTGG 4 cut(s) 20, 913, 1042, 1529
BssECI CCNNGG 6 cut(s) 96, 441, 1029, 1098, 1233, 1317
BssMI GATC 4 cut(s) 325, 479, 985, 1457
BssNI GRCGYC 1 cut(s) 1200
BssT1I CCWWGG 4 cut(s) 441, 1098, 1233, 1317
Bst2UI CCWGG 1 cut(s) 1277
Bst4CI ACNGT 2 cut(s) 108, 1509
Bst6I CTCTTC 4 cut(s) 660, 907, 912, 1036
BstACI GRCGYC 1 cut(s) 1200
BstAPI GCANNNNNTGC 1 cut(s) 1349
BstAUI TGTACA 1 cut(s) 937
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 298, 433
BstDEI CTNAG 6 cut(s) 636, 927, 1056, 1191, 1393, 1426
BstDSI CCRYGG 3 cut(s) 96, 1029, 1317
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 4 cut(s) 328, 482, 988, 1460
BstMAI GTCTC 4 cut(s) 596, 1132, 1217, 1453
BstMBI GATC 4 cut(s) 325, 479, 985, 1457
BstMWI GCNNNNNNNGC 2 cut(s) 1349, 1476
BstNI CCWGG 1 cut(s) 1277
BstNSI RCATGY 3 cut(s) 52, 300, 800
BstPAI GACNNNNGTC 1 cut(s) 1554
BstSCI CCNGG 3 cut(s) 278, 522, 1275
BstSFI CTRYAG 3 cut(s) 702, 849, 1600
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 4 cut(s) 287, 716, 1409, 1479
BstV2I GAAGAC 1 cut(s) 561
Bsu36I CCTNAGG 1 cut(s) 1426
BsuI GTATCC 1 cut(s) 811
BtgI CCRYGG 3 cut(s) 96, 1029, 1317
BtgZI GCGATG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 1095
BtsCI GGATG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 298, 433
CaiI CAGNNNCTG 2 cut(s) 662, 1400
CciI TCATGA 1 cut(s) 865
Cfr13I GGNCC 1 cut(s) 1430
Cfr42I CCGCGG 1 cut(s) 99
CseI GACGC 1 cut(s) 1189
Csp6I GTAC 1 cut(s) 938
CspCI CAANNNNNGTGG 2 cut(s) 952, 987
CviAII CATG 6 cut(s) 49, 297, 344, 797, 866, 1318
CviQI GTAC 1 cut(s) 938
DdeI CTNAG 6 cut(s) 636, 927, 1056, 1191, 1393, 1426
DpnI GATC 4 cut(s) 327, 481, 987, 1459
DpnII GATC 4 cut(s) 325, 479, 985, 1457
DraI TTTAAA 1 cut(s) 388
Eam1104I CTCTTC 4 cut(s) 660, 907, 912, 1036
EarI CTCTTC 4 cut(s) 660, 907, 912, 1036
Eco130I CCWWGG 4 cut(s) 441, 1098, 1233, 1317
Eco24I GRGCYC 1 cut(s) 644
Eco31I GGTCTC 1 cut(s) 1217
Eco47I GGWCC 1 cut(s) 1430
Eco57I CTGAAG 2 cut(s) 684, 729
Eco81I CCTNAGG 1 cut(s) 1426
Eco88I CYCGRG 1 cut(s) 1411
EcoO109I RGGNCCY 1 cut(s) 1430
EcoRII CCWGG 1 cut(s) 1275
EcoT14I CCWWGG 4 cut(s) 441, 1098, 1233, 1317
EcoT22I ATGCAT 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 644
ErhI CCWWGG 4 cut(s) 441, 1098, 1233, 1317
FaeI CATG 6 cut(s) 52, 300, 347, 800, 869, 1321
FaqI GGGAC 1 cut(s) 1416
FatI CATG 6 cut(s) 48, 296, 343, 796, 865, 1317
FauI CCCGC 2 cut(s) 91, 1490
Fnu4HI GCNGC 4 cut(s) 301, 705, 1398, 1468
FokI GGATG 1 cut(s) 299
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 4 cut(s) 301, 705, 1398, 1468
FspBI CTAG 2 cut(s) 893, 1376
GluI GCNGC 4 cut(s) 301, 705, 1398, 1468
HapII CCGG 4 cut(s) 279, 524, 1440, 1552
HgaI GACGC 1 cut(s) 1189
Hin1I GRCGYC 1 cut(s) 1200
Hin1II CATG 6 cut(s) 52, 300, 347, 800, 869, 1321
HincII GTYRAC 2 cut(s) 111, 1073
HindII GTYRAC 2 cut(s) 111, 1073
HpaII CCGG 4 cut(s) 279, 524, 1440, 1552
HphI GGTGA 2 cut(s) 743, 986
Hpy166II GTNNAC 4 cut(s) 111, 173, 938, 1073
Hpy8I GTNNAC 4 cut(s) 111, 173, 938, 1073
HpyAV CCTTC 8 cut(s) 124, 261, 286, 409, 679, 734, 1173, 1504
HpyCH4III ACNGT 2 cut(s) 108, 1509
HpyCH4IV ACGT 5 cut(s) 501, 786, 959, 966, 1094
HpyF10VI GCNNNNNNNGC 2 cut(s) 1349, 1476
HpyF3I CTNAG 6 cut(s) 636, 927, 1056, 1191, 1393, 1426
HpySE526I ACGT 5 cut(s) 501, 786, 959, 966, 1094
Hsp92I GRCGYC 1 cut(s) 1200
Hsp92II CATG 6 cut(s) 52, 300, 347, 800, 869, 1321
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 4 cut(s) 325, 479, 985, 1457
LmnI GCTCC 3 cut(s) 445, 1165, 1357
Lsp1109I GCAGC 4 cut(s) 287, 716, 1409, 1479
LweI GCATC 3 cut(s) 454, 1072, 1330
MaeI CTAG 2 cut(s) 893, 1376
MaeII ACGT 5 cut(s) 501, 786, 959, 966, 1094
MaeIII GTNAC 7 cut(s) 29, 160, 194, 904, 974, 1033, 1403
MalI GATC 4 cut(s) 327, 481, 987, 1459
MbiI CCGCTC 2 cut(s) 762, 1485
MboI GATC 4 cut(s) 325, 479, 985, 1457
MboII GAAGA 9 cut(s) 359, 524, 561, 677, 722, 924, 929, 1053, 1337
MfeI CAATTG 2 cut(s) 353, 495
MhlI GDGCHC 1 cut(s) 644
MlyI GAGTC 4 cut(s) 86, 677, 1391, 1564
MmeI TCCRAC 2 cut(s) 264, 1559
Mph1103I ATGCAT 1 cut(s) 345
MseI TTAA 4 cut(s) 69, 222, 387, 1239
MslI CAYNNNNRTG 2 cut(s) 815, 1080
MspA1I CMGCKG 3 cut(s) 98, 707, 1400
MspI CCGG 4 cut(s) 279, 524, 1440, 1552
MspR9I CCNGG 3 cut(s) 280, 524, 1277
MunI CAATTG 2 cut(s) 353, 495
Mva1269I GAATGC 2 cut(s) 878, 1339
MvaI CCWGG 1 cut(s) 1277
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 2 cut(s) 1349, 1476
NciI CCSGG 2 cut(s) 280, 524
NcoI CCATGG 1 cut(s) 1317
NdeII GATC 4 cut(s) 325, 479, 985, 1457
NlaIII CATG 6 cut(s) 52, 300, 347, 800, 869, 1321
NlaIV GGNNCC 3 cut(s) 447, 554, 1432
NmuCI GTSAC 3 cut(s) 29, 974, 1403
NsiI ATGCAT 1 cut(s) 345
NspI RCATGY 3 cut(s) 52, 300, 800
NspV TTCGAA 1 cut(s) 59
OliI CACNNNNGTG 1 cut(s) 815
PaeI GCATGC 1 cut(s) 300
PaeR7I CTCGAG 1 cut(s) 1411
PagI TCATGA 1 cut(s) 865
PciI ACATGT 1 cut(s) 796
PctI GAATGC 2 cut(s) 878, 1339
PfeI GAWTC 7 cut(s) 56, 61, 134, 515, 713, 1121, 1256
PflMI CCANNNNNTGG 1 cut(s) 26
PkrI GCNGC 4 cut(s) 302, 706, 1399, 1469
PleI GAGTC 4 cut(s) 86, 676, 1390, 1563
PpsI GAGTC 4 cut(s) 86, 676, 1390, 1563
PpuMI RGGWCCY 1 cut(s) 1430
PscI ACATGT 1 cut(s) 796
PshAI GACNNNNGTC 1 cut(s) 1554
Psp1406I AACGTT 1 cut(s) 966
Psp5II RGGWCCY 1 cut(s) 1430
Psp6I CCWGG 1 cut(s) 1275
PspGI CCWGG 1 cut(s) 1275
PspN4I GGNNCC 3 cut(s) 447, 554, 1432
PspPI GGNCC 1 cut(s) 1430
PspPPI RGGWCCY 1 cut(s) 1430
PstI CTGCAG 1 cut(s) 706
PstNI CAGNNNCTG 2 cut(s) 662, 1400
PvuII CAGCTG 2 cut(s) 707, 1400
RsaI GTAC 1 cut(s) 939
RsaNI GTAC 1 cut(s) 938
RseI CAYNNNNRTG 2 cut(s) 815, 1080
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 4 cut(s) 69, 222, 387, 1239
SatI GCNGC 4 cut(s) 301, 705, 1398, 1468
Sau3AI GATC 4 cut(s) 325, 479, 985, 1457
Sau96I GGNCC 1 cut(s) 1430
SchI GAGTC 4 cut(s) 86, 677, 1391, 1564
ScrFI CCNGG 3 cut(s) 280, 524, 1277
SduI GDGCHC 1 cut(s) 644
SfaNI GCATC 3 cut(s) 454, 1072, 1330
SfcI CTRYAG 3 cut(s) 702, 849, 1600
Sfr274I CTCGAG 1 cut(s) 1411
Sfr303I CCGCGG 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 59
SgrBI CCGCGG 1 cut(s) 99
SinI GGWCC 1 cut(s) 1430
SlaI CTCGAG 1 cut(s) 1411
SmiMI CAYNNNNRTG 2 cut(s) 815, 1080
SmlI CTYRAG 2 cut(s) 8, 1411
SmoI CTYRAG 2 cut(s) 8, 1411
SphI GCATGC 1 cut(s) 300
SsiI CCGC 5 cut(s) 96, 98, 762, 1483, 1564
SspMI CTAG 2 cut(s) 893, 1376
StyD4I CCNGG 3 cut(s) 278, 522, 1275
StyI CCWWGG 4 cut(s) 441, 1098, 1233, 1317
TaaI ACNGT 2 cut(s) 108, 1509
TaiI ACGT 5 cut(s) 504, 789, 962, 969, 1097
TaqI TCGA 6 cut(s) 59, 945, 1136, 1166, 1215, 1412
TatI WGTACW 1 cut(s) 937
TfiI GAWTC 7 cut(s) 56, 61, 134, 515, 713, 1121, 1256
Tru1I TTAA 4 cut(s) 69, 222, 387, 1239
Tru9I TTAA 4 cut(s) 69, 222, 387, 1239
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 3 cut(s) 29, 974, 1403
TseI GCWGC 4 cut(s) 300, 704, 1397, 1467
Tsp45I GTSAC 3 cut(s) 29, 974, 1403
TspDTI ATGAA 7 cut(s) 17, 360, 603, 872, 885, 1271, 1296
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 1 cut(s) 27
Van91I CCANNNNNTGG 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 1430
XceI RCATGY 3 cut(s) 52, 300, 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XhoI CTCGAG 1 cut(s) 1411
XspI CTAG 2 cut(s) 893, 1376
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.