Rorug04G0299700

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000995
Physical Location & Seq
Forward (+)
47436187 .. 47436904
718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0299700.1

Sequence Viewer

Length: 627 bp
ATGTCAAGTATTCTATGTTGGAATGTTAGAGGTGCTGCAGACTCAAAACTGCCTGGTATTATCCAGGATCTGAGAAGAATTTATAGATTTAATATCTTGGTGATTTCTGAGCCTCGAATTAGTGGAGACAAGGCTAAAAATGTTGCAAATTGCTTGGGCTTTTCGTCTTTCGCTATTGAGGATGCTATTGGTTTTTCTGGTGGTCTGTGGCTCTTATGGGATGAACGACAAGTGAAGTTAGAAGTGGTTGATAGTAATTTTCAGTCTATTACCTCTCTTGTCACTGAATCTTCGCATAAGCAATGGTTCTTCACTGCTGTCTATGCAAGTCCTGATCATGTTTTACGTGAGGAGCTTTGGGGGGGACGAGCTGTTTTGGGTGGGGGTAGTTTTGCAAATTGGGTTGACAGAAACTTCATGATTGATATGGGCTACATTGGTGCTGATTTTACATGGAAGGCTAACAGAAATGGGGAAGAGATCTTAGAGAGACTAGATAGAGGCCTCTGTAATATTTCATGGAGACATGAGTTTCCAGATGCCTTTGTTGAACATGTGGCAAGACTAAAATCTGATCATTGTCCTATTTTTCTCAGTTTGAGTAGAGCTCAAACCCTTCCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

23.59

Weight (kDa)

5.96

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 75
AcsI RAATTY 1 cut(s) 78
AflIII ACRYGT 1 cut(s) 553
AgsI TTSAA 1 cut(s) 551
AjnI CCWGG 2 cut(s) 52, 63
AluBI AGCT 3 cut(s) 355, 371, 608
AluI AGCT 3 cut(s) 355, 371, 608
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 3 cut(s) 120, 484, 517
AlwI GGATC 1 cut(s) 75
AlwNI CAGNNNCTG 1 cut(s) 70
AoxI GGCC 1 cut(s) 502
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 1 cut(s) 78
AsuHPI GGTGA 1 cut(s) 112
BanII GRGCYC 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 1 cut(s) 22
BciT130I CCWGG 2 cut(s) 54, 65
BclI TGATCA 2 cut(s) 334, 574
BcoDI GTCTC 3 cut(s) 120, 484, 517
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 1 cut(s) 36
BglII AGATCT 1 cut(s) 480
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
Bme1390I CCNGG 2 cut(s) 54, 65
BmrFI CCNGG 2 cut(s) 54, 65
BmsI GCATC 2 cut(s) 172, 529
BplI GAGNNNNNCTC 2 cut(s) 592, 624
BsaAI YACGTR 1 cut(s) 347
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 2 cut(s) 54, 65
BseGI GGATG 2 cut(s) 187, 226
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 3 cut(s) 62, 99, 607
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 1 cut(s) 22
BshFI GGCC 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 610
BslFI GGGAC 1 cut(s) 378
BsmAI GTCTC 3 cut(s) 120, 484, 517
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 4 cut(s) 67, 334, 480, 574
BspANI GGCC 1 cut(s) 504
BspCNI CTCAG 3 cut(s) 63, 100, 606
BspHI TCATGA 1 cut(s) 417
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 308
BssMI GATC 4 cut(s) 67, 334, 480, 574
Bst2UI CCWGG 2 cut(s) 54, 65
Bst6I CTCTTC 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 347
BstDEI CTNAG 4 cut(s) 71, 108, 484, 593
BstF5I GGATG 2 cut(s) 187, 226
BstKTI GATC 4 cut(s) 70, 337, 483, 577
BstMAI GTCTC 3 cut(s) 120, 484, 517
BstMBI GATC 4 cut(s) 67, 334, 480, 574
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstNI CCWGG 2 cut(s) 54, 65
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 52, 63
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 2 cut(s) 67, 480
BstYI RGATCY 2 cut(s) 67, 480
BsuRI GGCC 1 cut(s) 504
BtsCI GGATG 2 cut(s) 187, 226
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 282, 312
CaiI CAGNNNCTG 1 cut(s) 70
CciI TCATGA 1 cut(s) 417
CviAII CATG 6 cut(s) 338, 418, 453, 519, 527, 554
DdeI CTNAG 4 cut(s) 71, 108, 484, 593
DpnI GATC 4 cut(s) 69, 336, 482, 576
DpnII GATC 4 cut(s) 67, 334, 480, 574
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Ecl136II GAGCTC 1 cut(s) 608
Eco147I AGGCCT 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 608
EcoICRI GAGCTC 1 cut(s) 608
EcoRII CCWGG 2 cut(s) 52, 63
EcoT38I GRGCYC 1 cut(s) 610
FaeI CATG 6 cut(s) 341, 421, 456, 522, 530, 557
FaqI GGGAC 1 cut(s) 378
FatI CATG 6 cut(s) 337, 417, 452, 518, 526, 553
FbaI TGATCA 2 cut(s) 334, 574
Fnu4HI GCNGC 1 cut(s) 36
FokI GGATG 2 cut(s) 194, 233
FriOI GRGCYC 1 cut(s) 610
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 1 cut(s) 494
GluI GCNGC 1 cut(s) 36
HaeIII GGCC 1 cut(s) 504
Hin1II CATG 6 cut(s) 341, 421, 456, 522, 530, 557
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HinfI GANTC 2 cut(s) 41, 287
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 3 cut(s) 72, 109, 574
Hpy188III TCNNGA 3 cut(s) 332, 418, 536
Hpy8I GTNNAC 1 cut(s) 406
HpyAV CCTTC 2 cut(s) 451, 626
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 4 cut(s) 38, 146, 326, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 4 cut(s) 71, 108, 484, 593
HpySE526I ACGT 1 cut(s) 346
Hsp92II CATG 6 cut(s) 341, 421, 456, 522, 530, 557
Ksp22I TGATCA 2 cut(s) 334, 574
Kzo9I GATC 4 cut(s) 67, 334, 480, 574
LmnI GCTCC 1 cut(s) 352
LpnPI CCDG 7 cut(s) 39, 50, 66, 77, 183, 345, 549
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 2 cut(s) 172, 529
MaeI CTAG 1 cut(s) 494
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 280
MalI GATC 4 cut(s) 69, 336, 482, 576
MboI GATC 4 cut(s) 67, 334, 480, 574
MboII GAAGA 4 cut(s) 87, 282, 301, 488
MflI RGATCY 2 cut(s) 67, 480
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 5 cut(s) 78, 117, 148, 256, 397
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 7 cut(s) 23, 123, 172, 283, 343, 494, 515
MseI TTAA 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 54, 65
MvaI CCWGG 2 cut(s) 54, 65
MwoI GCNNNNNNNGC 1 cut(s) 323
NdeII GATC 4 cut(s) 67, 334, 480, 574
NlaIII CATG 6 cut(s) 341, 421, 456, 522, 530, 557
NmuCI GTSAC 1 cut(s) 280
NspI RCATGY 1 cut(s) 557
PagI TCATGA 1 cut(s) 417
PceI AGGCCT 1 cut(s) 504
PciI ACATGT 1 cut(s) 553
PfeI GAWTC 1 cut(s) 287
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 1 cut(s) 37
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
Ppu21I YACGTR 1 cut(s) 347
PscI ACATGT 1 cut(s) 553
Psp124BI GAGCTC 1 cut(s) 610
Psp6I CCWGG 2 cut(s) 52, 63
PspGI CCWGG 2 cut(s) 52, 63
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 70
PsuI RGATCY 2 cut(s) 67, 480
SacI GAGCTC 1 cut(s) 610
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 4 cut(s) 67, 334, 480, 574
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 2 cut(s) 54, 65
SduI GDGCHC 1 cut(s) 610
SetI ASST 6 cut(s) 34, 275, 349, 357, 373, 610
SfaNI GCATC 2 cut(s) 172, 529
SfcI CTRYAG 1 cut(s) 36
Sse9I AATT 5 cut(s) 78, 117, 148, 256, 397
SseBI AGGCCT 1 cut(s) 504
SspI AATATT 1 cut(s) 514
SspMI CTAG 1 cut(s) 494
SstI GAGCTC 1 cut(s) 610
StuI AGGCCT 1 cut(s) 504
StyD4I CCNGG 2 cut(s) 52, 63
TaiI ACGT 1 cut(s) 349
TaqI TCGA 1 cut(s) 115
TasI AATT 5 cut(s) 78, 117, 148, 256, 397
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 2 cut(s) 289, 319
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 3 cut(s) 237, 406, 507
TspGWI ACGGA 1 cut(s) 609
TspRI CASTG 2 cut(s) 289, 319
XapI RAATTY 1 cut(s) 78
XceI RCATGY 1 cut(s) 557
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.