FvH4_4g30760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001487
Physical Location & Seq
Forward (+)
30232707 .. 30233414
708 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30760.t1

Sequence Viewer

Length: 519 bp
ATGGCTGGTGATCATCGTATACTCAACTCTTTGATTTTCACGATCATCTTCTCCCTCTTCTTATCACTCCCCATTTCTGTAGAAAGCTCATGGCGTTGCCTCGGCGCCCAGGAGTACAAAACCACCGTCCAGCAAGAGTTCGAGCAAGACTTCAGAAATGCACACCCAGACCATTACAACACCTTCACAGTTGAGTGTGAGGCTATGAACTCGTTAACAGTCTCCCTGGACTTGAAGGTGAAAGCTGAATCTAAAAACGGCATTGCAAAGGTGTCAACATGTGGGGGTTGGTTTGTTGATCCAAAGTACAGATGCCAAGTATATCTGAACGACGGTAGCTGGAATGGAATGTTCCTGCCATTGTTGAAAGAGTACGACTGTGTTGAAAAGAACCACCACTGCTGGTGGAAGCTGCATGACGGGTACTTACTTCGTCACTCGCCGTCGCGAAACGCCTGGCTTCGCCAGAACTATGAGCTCGGCTGGGTTCCGCAACCCACACCTGAAGACATACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

20.03

Weight (kDa)

5.95

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 19
AccII CGCG 1 cut(s) 448
AciI CCGC 1 cut(s) 491
AclWI GGATC 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 4 cut(s) 116, 308, 374, 425
AflIII ACRYGT 1 cut(s) 278
AgsI TTSAA 3 cut(s) 235, 367, 386
AjnI CCWGG 3 cut(s) 108, 225, 455
AluBI AGCT 5 cut(s) 87, 245, 339, 412, 478
AluI AGCT 5 cut(s) 87, 245, 339, 412, 478
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 1 cut(s) 226
AlwI GGATC 1 cut(s) 293
ApeKI GCWGC 1 cut(s) 412
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
AspLEI GCGC 1 cut(s) 107
AsuHPI GGTGA 2 cut(s) 20, 250
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 1 cut(s) 399
BceAI ACGGC 2 cut(s) 274, 427
BciT130I CCWGG 3 cut(s) 110, 227, 457
BclI TGATCA 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 226
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 1 cut(s) 413
BlsI GCNGC 1 cut(s) 414
Bme1390I CCNGG 3 cut(s) 110, 227, 457
BmiI GGNNCC 2 cut(s) 106, 489
BmrFI CCNGG 3 cut(s) 110, 227, 457
BmsI GCATC 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 100, 108, 225
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 3 cut(s) 110, 227, 457
BseDI CCNNGG 3 cut(s) 100, 108, 225
BseMI GCAATG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 399
BseYI CCCAGC 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 448
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 480
BsmAI GTCTC 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 3 cut(s) 10, 42, 298
Bsp68I TCGCGA 1 cut(s) 448
BspACI CCGC 1 cut(s) 491
BspFNI CGCG 1 cut(s) 448
BspLI GGNNCC 2 cut(s) 106, 489
BspPI GGATC 1 cut(s) 293
BspT107I GGYRCC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 261
BssECI CCNNGG 3 cut(s) 100, 108, 225
BssMI GATC 3 cut(s) 10, 42, 298
BssNAI GTATAC 1 cut(s) 20
BssNI GRCGYC 1 cut(s) 105
Bst1107I GTATAC 1 cut(s) 20
Bst2UI CCWGG 3 cut(s) 110, 227, 457
Bst4CI ACNGT 6 cut(s) 127, 190, 220, 335, 380, 516
Bst6I CTCTTC 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 105
BstFNI CGCG 1 cut(s) 448
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 3 cut(s) 13, 45, 301
BstMAI GTCTC 1 cut(s) 226
BstMBI GATC 3 cut(s) 10, 42, 298
BstNI CCWGG 3 cut(s) 110, 227, 457
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 3 cut(s) 108, 225, 455
BstSFI CTRYAG 1 cut(s) 78
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 1 cut(s) 399
BstV2I GAAGAC 1 cut(s) 513
BstZ17I GTATAC 1 cut(s) 20
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 397
BtuMI TCGCGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 107
Csp6I GTAC 4 cut(s) 115, 307, 373, 424
CviAII CATG 3 cut(s) 90, 279, 416
CviJI RGCY 9 cut(s) 5, 87, 203, 245, 339, 412, 460, 478, 483
CviKI_1 RGCY 9 cut(s) 5, 87, 203, 245, 339, 412, 460, 478, 483
CviQI GTAC 4 cut(s) 115, 307, 373, 424
DinI GGCGCC 1 cut(s) 106
DpnI GATC 3 cut(s) 12, 44, 300
DpnII GATC 3 cut(s) 10, 42, 298
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 478
Eco24I GRGCYC 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 478
EcoRII CCWGG 3 cut(s) 108, 225, 455
EcoT38I GRGCYC 1 cut(s) 480
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
FaeI CATG 3 cut(s) 93, 282, 419
FaiI YATR 8 cut(s) 20, 91, 206, 280, 322, 417, 474, 512
FatI CATG 3 cut(s) 89, 278, 415
FbaI TGATCA 1 cut(s) 10
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 413
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 1 cut(s) 413
GlaI GCGC 1 cut(s) 106
GluI GCNGC 1 cut(s) 413
GsaI CCCAGC 1 cut(s) 487
HaeII RGCGCY 1 cut(s) 108
HhaI GCGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 3 cut(s) 93, 282, 419
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 2 cut(s) 216, 276
HindII GTYRAC 2 cut(s) 216, 276
HinfI GANTC 1 cut(s) 248
HpaI GTTAAC 1 cut(s) 216
HphI GGTGA 2 cut(s) 20, 250
Hpy166II GTNNAC 3 cut(s) 20, 216, 276
Hpy188I TCNGA 2 cut(s) 155, 327
Hpy188III TCNNGA 2 cut(s) 40, 447
Hpy8I GTNNAC 3 cut(s) 20, 216, 276
Hpy99I CGWCG 2 cut(s) 335, 448
HpyAV CCTTC 2 cut(s) 193, 229
HpyCH4III ACNGT 6 cut(s) 127, 190, 220, 335, 380, 516
HpyCH4V TGCA 3 cut(s) 161, 266, 415
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 3 cut(s) 93, 282, 419
HspAI GCGC 1 cut(s) 105
KasI GGCGCC 1 cut(s) 104
Ksp22I TGATCA 1 cut(s) 10
KspAI GTTAAC 1 cut(s) 216
Kzo9I GATC 3 cut(s) 10, 42, 298
Lsp1109I GCAGC 1 cut(s) 399
LweI GCATC 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 434
MalI GATC 3 cut(s) 12, 44, 300
MboI GATC 3 cut(s) 10, 42, 298
MboII GAAGA 3 cut(s) 40, 49, 518
MhlI GDGCHC 1 cut(s) 480
Mly113I GGCGCC 1 cut(s) 105
MnlI CCTC 3 cut(s) 65, 110, 193
MseI TTAA 1 cut(s) 215
MspR9I CCNGG 3 cut(s) 110, 227, 457
MvaI CCWGG 3 cut(s) 110, 227, 457
MvnI CGCG 1 cut(s) 448
NarI GGCGCC 1 cut(s) 105
NdeII GATC 3 cut(s) 10, 42, 298
NlaIII CATG 3 cut(s) 93, 282, 419
NlaIV GGNNCC 2 cut(s) 106, 489
NmeAIII GCCGAG 2 cut(s) 81, 459
NmuCI GTSAC 1 cut(s) 434
NruI TCGCGA 1 cut(s) 448
NspI RCATGY 1 cut(s) 282
PciI ACATGT 1 cut(s) 278
PfeI GAWTC 1 cut(s) 248
PkrI GCNGC 1 cut(s) 414
PluTI GGCGCC 1 cut(s) 108
PscI ACATGT 1 cut(s) 278
Psp124BI GAGCTC 1 cut(s) 480
Psp6I CCWGG 3 cut(s) 108, 225, 455
PspFI CCCAGC 1 cut(s) 483
PspGI CCWGG 3 cut(s) 108, 225, 455
PspN4I GGNNCC 2 cut(s) 106, 489
RruI TCGCGA 1 cut(s) 448
RsaI GTAC 4 cut(s) 116, 308, 374, 425
RsaNI GTAC 4 cut(s) 115, 307, 373, 424
SacI GAGCTC 1 cut(s) 480
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 1 cut(s) 413
Sau3AI GATC 3 cut(s) 10, 42, 298
ScrFI CCNGG 3 cut(s) 110, 227, 457
SduI GDGCHC 1 cut(s) 480
SetI ASST 9 cut(s) 89, 185, 240, 247, 273, 341, 414, 480, 505
SfaNI GCATC 1 cut(s) 302
SfcI CTRYAG 1 cut(s) 78
SfoI GGCGCC 1 cut(s) 106
SsiI CCGC 1 cut(s) 491
SspDI GGCGCC 1 cut(s) 104
SstI GAGCTC 1 cut(s) 480
StyD4I CCNGG 3 cut(s) 108, 225, 455
TaaI ACNGT 6 cut(s) 127, 190, 220, 335, 380, 516
TaqI TCGA 1 cut(s) 141
TatI WGTACW 2 cut(s) 114, 306
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 1 cut(s) 404
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 1 cut(s) 221
TspRI CASTG 1 cut(s) 404
XceI RCATGY 1 cut(s) 282
XmiI GTMKAC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.