Rh4DG373200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001487
Physical Location & Seq
Forward (+)
59106866 .. 59107487
622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG373200.1

Sequence Viewer

Length: 528 bp
ATGGGTGGTGATCATTTTCATTTAGTTAATTACTATCTGATTTGTACGATCATCTTCTCCCTCTTCTTATCATTCCCCATATCTGTAGAAAGCTCGTGGAGTTGCCTCGGTGCCCAGGAGTACAAAACCACCGTCCAACAAGAGTTCGAGCAAGACTTCAGAAATGCACACCCAAATAATTACAATAGCTTCAGAGTTGAGTGTGAGGCTATGAACTCGTTAGCGGTCACCCTGGACTTGAAGGTTACTGCGGAATCTAATTCCAACGGCATTGCGGAGTTGAAGACATGTGGGGGGTGGTTCGTTGACCCAAAGTACAGATGCCTAGTATATTTGGAAGACGGTACCTGGAATGGGTTGTTCCAGCCATTGCTGCCGGAGTACGACTGTGTCGAAAAGAACCACCACTGCTGGTGGAAGCTGCATGACGGGTATTTACTTCGCCACTCGCCCAAGCGAAACGGCTGGCTTCGTCAAAATTATGGCCTCGGCTGGGTTCCGCAACCCACAGCTGAAGACATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

20.3

Weight (kDa)

5.59

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 344
AccB1I GGYRCC 2 cut(s) 110, 344
AciI CCGC 4 cut(s) 224, 251, 275, 500
AcuI CTGAAG 2 cut(s) 142, 175
AfaI GTAC 5 cut(s) 46, 122, 317, 346, 383
AfiI CCNNNNNNNGG 2 cut(s) 354, 493
AflIII ACRYGT 1 cut(s) 287
AgsI TTSAA 2 cut(s) 241, 283
AjnI CCWGG 3 cut(s) 114, 231, 347
AluBI AGCT 4 cut(s) 93, 189, 421, 512
AluI AGCT 4 cut(s) 93, 189, 421, 512
AoxI GGCC 1 cut(s) 484
ApeKI GCWGC 2 cut(s) 373, 421
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
Asp718I GGTACC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 20, 220
BaeGI GKGCMC 1 cut(s) 115
BanI GGYRCC 2 cut(s) 110, 344
BauI CACGAG 1 cut(s) 94
BbsI GAAGAC 3 cut(s) 290, 345, 522
BbvI GCAGC 2 cut(s) 360, 408
BceAI ACGGC 2 cut(s) 283, 478
BciT130I CCWGG 3 cut(s) 116, 233, 349
BclI TGATCA 1 cut(s) 10
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 84
BisI GCNGC 2 cut(s) 374, 422
BlsI GCNGC 2 cut(s) 375, 423
Bme1390I CCNGG 3 cut(s) 116, 233, 349
BmiI GGNNCC 3 cut(s) 112, 346, 498
BmrFI CCNGG 3 cut(s) 116, 233, 349
BmsI GCATC 1 cut(s) 311
BpiI GAAGAC 3 cut(s) 290, 345, 522
BsaJI CCNNGG 4 cut(s) 106, 114, 231, 487
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 493
Bse3DI GCAATG 2 cut(s) 270, 368
BseBI CCWGG 3 cut(s) 116, 233, 349
BseDI CCNNGG 4 cut(s) 106, 114, 231, 487
BseLI CCNNNNNNNGG 2 cut(s) 354, 493
BseMI GCAATG 2 cut(s) 270, 368
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 2 cut(s) 360, 408
BseYI CCCAGC 1 cut(s) 492
BshFI GGCC 1 cut(s) 486
BshNI GGYRCC 2 cut(s) 110, 344
BsiSI CCGG 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 354, 493
BsnI GGCC 1 cut(s) 486
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 10, 48
BspACI CCGC 4 cut(s) 224, 251, 275, 500
BspANI GGCC 1 cut(s) 486
BspLI GGNNCC 3 cut(s) 112, 346, 498
BspT107I GGYRCC 2 cut(s) 110, 344
BsrDI GCAATG 2 cut(s) 270, 368
BssECI CCNNGG 4 cut(s) 106, 114, 231, 487
BssMI GATC 2 cut(s) 10, 48
BssSI CACGAG 1 cut(s) 94
Bst2BI CACGAG 1 cut(s) 94
Bst2UI CCWGG 3 cut(s) 116, 233, 349
Bst4CI ACNGT 3 cut(s) 133, 344, 389
Bst6I CTCTTC 1 cut(s) 68
BstC8I GCNNGC 1 cut(s) 467
BstEII GGTNACC 1 cut(s) 226
BstKTI GATC 2 cut(s) 13, 51
BstMBI GATC 2 cut(s) 10, 48
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstNI CCWGG 3 cut(s) 116, 233, 349
BstNSI RCATGY 1 cut(s) 291
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 3 cut(s) 114, 231, 347
BstSFI CTRYAG 1 cut(s) 84
BstSLI GKGCMC 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 360, 408
BstV2I GAAGAC 3 cut(s) 290, 345, 522
BsuRI GGCC 1 cut(s) 486
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 1 cut(s) 467
Csp6I GTAC 5 cut(s) 45, 121, 316, 345, 382
CviAII CATG 2 cut(s) 288, 425
CviQI GTAC 5 cut(s) 45, 121, 316, 345, 382
DpnI GATC 2 cut(s) 12, 50
DpnII GATC 2 cut(s) 10, 48
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 142, 175
Eco91I GGTNACC 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 226
EcoRII CCWGG 3 cut(s) 114, 231, 347
FaeI CATG 2 cut(s) 291, 428
FaiI YATR 7 cut(s) 80, 212, 289, 331, 426, 483, 521
FatI CATG 2 cut(s) 287, 424
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 2 cut(s) 374, 422
Fsp4HI GCNGC 2 cut(s) 374, 422
FspBI CTAG 1 cut(s) 326
GluI GCNGC 2 cut(s) 374, 422
GsaI CCCAGC 1 cut(s) 496
HaeIII GGCC 1 cut(s) 486
HapII CCGG 1 cut(s) 377
Hin1II CATG 2 cut(s) 291, 428
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 1 cut(s) 254
HpaII CCGG 1 cut(s) 377
HphI GGTGA 2 cut(s) 20, 220
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 3 cut(s) 39, 161, 194
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 3 cut(s) 133, 344, 389
HpyCH4V TGCA 2 cut(s) 167, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
Hsp92II CATG 2 cut(s) 291, 428
KpnI GGTACC 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 2 cut(s) 10, 48
Lsp1109I GCAGC 2 cut(s) 360, 408
LweI GCATC 1 cut(s) 311
MaeI CTAG 1 cut(s) 326
MaeIII GTNAC 2 cut(s) 226, 244
MalI GATC 2 cut(s) 12, 50
MboI GATC 2 cut(s) 10, 48
MboII GAAGA 5 cut(s) 46, 55, 295, 350, 527
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 4 cut(s) 28, 178, 259, 478
MmeI TCCRAC 2 cut(s) 160, 288
MnlI CCTC 4 cut(s) 71, 116, 199, 497
MseI TTAA 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 1 cut(s) 377
MspR9I CCNGG 3 cut(s) 116, 233, 349
MvaI CCWGG 3 cut(s) 116, 233, 349
MwoI GCNNNNNNNGC 1 cut(s) 373
NdeII GATC 2 cut(s) 10, 48
NlaIII CATG 2 cut(s) 291, 428
NlaIV GGNNCC 3 cut(s) 112, 346, 498
NmeAIII GCCGAG 1 cut(s) 468
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 1 cut(s) 291
PciI ACATGT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PfeI GAWTC 1 cut(s) 254
PflFI GACNNNGTC 1 cut(s) 389
PkrI GCNGC 2 cut(s) 375, 423
PscI ACATGT 1 cut(s) 287
Psp6I CCWGG 3 cut(s) 114, 231, 347
PspEI GGTNACC 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 492
PspGI CCWGG 3 cut(s) 114, 231, 347
PspN4I GGNNCC 3 cut(s) 112, 346, 498
PsyI GACNNNGTC 1 cut(s) 389
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 5 cut(s) 46, 122, 317, 346, 383
RsaNI GTAC 5 cut(s) 45, 121, 316, 345, 382
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 2 cut(s) 374, 422
Sau3AI GATC 2 cut(s) 10, 48
ScrFI CCNGG 3 cut(s) 116, 233, 349
SduI GDGCHC 1 cut(s) 115
SetI ASST 6 cut(s) 95, 191, 246, 350, 423, 514
SfaNI GCATC 1 cut(s) 311
SfcI CTRYAG 1 cut(s) 84
Sse9I AATT 4 cut(s) 28, 178, 259, 478
SsiI CCGC 4 cut(s) 224, 251, 275, 500
SspMI CTAG 1 cut(s) 326
StyD4I CCNGG 3 cut(s) 114, 231, 347
TaaI ACNGT 3 cut(s) 133, 344, 389
TaqI TCGA 2 cut(s) 147, 393
TasI AATT 4 cut(s) 28, 178, 259, 478
TatI WGTACW 2 cut(s) 120, 315
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 413
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 373, 421
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 2 cut(s) 8, 227
TspRI CASTG 1 cut(s) 413
Tth111I GACNNNGTC 1 cut(s) 389
XceI RCATGY 1 cut(s) 291
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.