Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001487

Physical Location & Seq

Genomic Coordinates Reverse (-)

29259198 .. 29261173

Gene Structure 1976 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1204800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 672 bp

ATGAGTGAGGTGTTTGAGGGGTACGAGCGCCAATACTGCGAGCTCTCAGCCAATCTCTCAAGCAAGTGCACGGCTGCCACCGCCGTGGATGGAGGATGTGAGAAAAAGAATCAGATAATTGCAGAGGTAAAAGCTGGATTTGAGGATGCAGATACTTTGCTTAGGAATATGGACCTTGAGGCAAGGAGTTTGCAGCCAAGTGTGAAGGCAGCTCTTCTTGCTAAGCTAAGAGGATACAAGGCTGATTTGAACAATTTGAAGAGCCAACTGAAGAGAATGGCATCACCTGTCGTGAATTTCGCAGCTCGGGATGAACTGTTGGAGCCCGGATCGGCAGATTCCTTGACGACATCGAATGATCAACGAGGAAGATTGTTGATGACGACTGACAGGTTAAATCAGTCGACGGACAGAATCAAGGAGAGTAGAAGAGCAATGCTGGAAACCGAGGAGCTTGGGGTCTCAATCCTCCAAGATTTGCACCAACAACGCCAGTCACTTCTACATGCTCACAATACACTCCGCGGGGCGGATGAAAGTATCAGCAAGAGCAAGAAGGTGCTGACCGCCATGACGAGGAGTATGAACAGGAACAAATGGATTGTCGGCTCCATCATCGGAGTTTTTGCCGTTGCAATCTTGTTTATCCTTTATCTTAAGATTACGCGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005783 GO:0005789 GO:0005794 GO:0005798 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006888 GO:0006892 GO:0006896 GO:0006906 GO:0006996 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0010008 GO:0012505 GO:0012506 GO:0012507 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016197 GO:0016482 GO:0030133 GO:0030134 GO:0030135 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0031090 GO:0031201 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042147 GO:0042175 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048280 GO:0048284 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090174 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko04130 ko04138 map04130 map04138

Pfam Domains

Protein Families

V-SNARE V-SNARE_C

Protein Analysis

224

Amino Acids

24.82

Weight (kDa)

9.13

Isoelectric Point (pI)

43.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
V-SNARE	PF05008	1 - 92	6.4e-28	Vesicle transport v-SNARE protein N-terminus
V-SNARE_C	PF12352	130 - 193	4.7e-20	Snare region anchored in the vesicle membrane C-terminus
Sec20	PF03908	133 - 220	3e-07	Sec20

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001487)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29100 AT3G29100 AT3G29100 AT3G29100 AT3G29100 AT5G39510
fragaria_vesca	FvH4_4g30760 FvH4_5g14550 FvH4_5g14550 FvH4_6g47910
malus_domestica	MD09G1055700.v1.1 MD14G1204800.v1.1 MD17G1053400.v1.1
prunus_persica	Prupe.3G263300_v2.0.a1 Prupe.5G198100_v2.0.a1
pyrus_communis	pycom111g04660 pycom14g16920 pycom17g05120
rosa_chinensis	RchiOBHm_Chr2g0167111 RchiOBHm_Chr7g0182011 RchiOBHm_Chr7g0182061
rosa_laevigata	RLG00000005160
rosa_multiflora	Rmu_co8218632.1_g000001 Rmu_co8397643.1_g000001 Rmu_sc0003511.1_g000026 Rmu_sc0003511.1_g000043 Rmu_ssc0000297.1_g000004
rosa_roxburghii	Rroxscaffold_2G00083710 Rroxscaffold_3G00271540 Rroxscaffold_3G00271610 Rroxscaffold_3G00271620
rosa_rugosa	Rorug02G0528500 Rorug06G0445400 Rorug06G0445400
rosa_samantha	Rh2AG595600 Rh2BG606600 Rh2CG577600 Rh2DG618300 Rh4BG378600 Rh4CG392900 Rh4DG373200 Rh7AG047600 Rh7BG047700 Rh7CG047800 Rh7DG046900
rosa_wichuraiana	Rw0G000450 Rw0G007530 Rw0G011700 Rw0G019700 Rw2G049580 Rw7G003940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	404
AccII	CGCG	2 cut(s)	525, 667
AciI	CCGC	5 cut(s)	81, 523, 525, 530, 567
AclWI	GGATC	1 cut(s)	337
AcsI	RAATTY	1 cut(s)	295
AcuI	CTGAAG	1 cut(s)	290
AfaI	GTAC	1 cut(s)	23
AfiI	CCNNNNNNNGG	2 cut(s)	529, 576
AflII	CTTAAG	1 cut(s)	656
AgsI	TTSAA	2 cut(s)	250, 259
AleI	CACNNNNGTG	1 cut(s)	83
AluBI	AGCT	6 cut(s)	43, 134, 212, 226, 305, 454
AluI	AGCT	6 cut(s)	43, 134, 212, 226, 305, 454
Alw21I	GWGCWC	2 cut(s)	45, 71
Alw26I	GTCTC	1 cut(s)	466
Alw44I	GTGCAC	1 cut(s)	67
AlwI	GGATC	1 cut(s)	337
Ama87I	CYCGRG	1 cut(s)	306
ApaLI	GTGCAC	1 cut(s)	67
ApeKI	GCWGC	4 cut(s)	74, 193, 209, 302
ApoI	RAATTY	1 cut(s)	295
ArsI	GACNNNNNNTTYG	2 cut(s)	588, 620
AspLEI	GCGC	2 cut(s)	30, 669
AspS9I	GGNCC	1 cut(s)	172
AsuC2I	CCSGG	1 cut(s)	327
AsuHPI	GGTGA	1 cut(s)	276
AvaI	CYCGRG	1 cut(s)	306
AvaII	GGWCC	1 cut(s)	172
BaeGI	GKGCMC	1 cut(s)	71
BanII	GRGCYC	2 cut(s)	45, 327
Bbv12I	GWGCWC	2 cut(s)	45, 71
BbvI	GCAGC	4 cut(s)	61, 205, 221, 314
BccI	CCATC	2 cut(s)	83, 620
BceAI	ACGGC	3 cut(s)	68, 87, 614
BciVI	GTATCC	1 cut(s)	227
BclI	TGATCA	1 cut(s)	358
BcnI	CCSGG	1 cut(s)	327
BcoDI	GTCTC	1 cut(s)	466
BfaI	CTAG	1 cut(s)	670
BfoI	RGCGCY	1 cut(s)	31
BfrI	CTTAAG	1 cut(s)	656
BfuI	GTATCC	1 cut(s)	227
BisI	GCNGC	4 cut(s)	75, 194, 210, 303
BlpI	GCTNAGC	1 cut(s)	222
BlsI	GCNGC	4 cut(s)	76, 195, 211, 304
Bme1390I	CCNGG	1 cut(s)	327
Bme18I	GGWCC	1 cut(s)	172
BmeT110I	CYCGRG	1 cut(s)	306
BmgT120I	GGNCC	1 cut(s)	172
BmiI	GGNNCC	2 cut(s)	324, 610
BmrFI	CCNGG	1 cut(s)	327
BmsI	GCATC	2 cut(s)	136, 290
Bpu10I	CCTNAGC	1 cut(s)	161
Bpu1102I	GCTNAGC	1 cut(s)	222
BpuEI	CTTGAG	2 cut(s)	43, 197
BpuMI	CCSGG	1 cut(s)	327
BsaI	GGTCTC	1 cut(s)	466
BsaJI	CCNNGG	3 cut(s)	84, 447, 523
Bsc4I	CCNNNNNNNGG	2 cut(s)	529, 576
Bse1I	ACTGG	1 cut(s)	493
Bse3DI	GCAATG	1 cut(s)	441
BseDI	CCNNGG	3 cut(s)	84, 447, 523
BseGI	GGATG	5 cut(s)	94, 101, 151, 316, 538
BseLI	CCNNNNNNNGG	2 cut(s)	529, 576
BseMI	GCAATG	1 cut(s)	441
BseMII	CTCAG	1 cut(s)	60
BseNI	ACTGG	1 cut(s)	493
BseRI	GAGGAG	2 cut(s)	464, 592
BseSI	GKGCMC	1 cut(s)	71
BseXI	GCAGC	4 cut(s)	61, 205, 221, 314
Bsh1236I	CGCG	2 cut(s)	525, 667
BsiHKAI	GWGCWC	2 cut(s)	45, 71
BsiHKCI	CYCGRG	1 cut(s)	306
BsiSI	CCGG	1 cut(s)	327
BslI	CCNNNNNNNGG	2 cut(s)	529, 576
BsmAI	GTCTC	1 cut(s)	466
Bso31I	GGTCTC	1 cut(s)	466
BsoBI	CYCGRG	1 cut(s)	306
Bsp1286I	GDGCHC	3 cut(s)	45, 71, 327
Bsp143I	GATC	2 cut(s)	329, 358
Bsp1720I	GCTNAGC	1 cut(s)	222
BspACI	CCGC	5 cut(s)	81, 523, 525, 530, 567
BspCNI	CTCAG	1 cut(s)	59
BspFNI	CGCG	2 cut(s)	525, 667
BspLI	GGNNCC	2 cut(s)	324, 610
BspPI	GGATC	1 cut(s)	337
BspQI	GCTCTTC	3 cut(s)	219, 254, 424
BspTI	CTTAAG	1 cut(s)	656
BspTNI	GGTCTC	1 cut(s)	466
BsrDI	GCAATG	1 cut(s)	441
BsrI	ACTGG	1 cut(s)	493
BssECI	CCNNGG	3 cut(s)	84, 447, 523
BssMI	GATC	2 cut(s)	329, 358
Bst4CI	ACNGT	1 cut(s)	318
Bst6I	CTCTTC	4 cut(s)	219, 254, 266, 424
BstAFI	CTTAAG	1 cut(s)	656
BstC8I	GCNNGC	1 cut(s)	41
BstDEI	CTNAG	4 cut(s)	46, 161, 222, 227
BstDSI	CCRYGG	2 cut(s)	84, 523
BstF5I	GGATG	5 cut(s)	94, 101, 151, 316, 538
BstFNI	CGCG	2 cut(s)	525, 667
BstH2I	RGCGCY	1 cut(s)	31
BstHHI	GCGC	2 cut(s)	30, 669
BstKTI	GATC	2 cut(s)	332, 361
BstMAI	GTCTC	1 cut(s)	466
BstMBI	GATC	2 cut(s)	329, 358
BstMWI	GCNNNNNNNGC	3 cut(s)	36, 80, 218
BstNSI	RCATGY	1 cut(s)	509
BstSCI	CCNGG	1 cut(s)	325
BstSLI	GKGCMC	1 cut(s)	71
BstUI	CGCG	2 cut(s)	525, 667
BstV1I	GCAGC	4 cut(s)	61, 205, 221, 314
BstXI	CCANNNNNNTGG	1 cut(s)	85
BsuI	GTATCC	1 cut(s)	227
BtgI	CCRYGG	2 cut(s)	84, 523
BtsCI	GGATG	5 cut(s)	94, 101, 151, 316, 538
Cac8I	GCNNGC	1 cut(s)	41
CfoI	GCGC	2 cut(s)	30, 669
Cfr13I	GGNCC	1 cut(s)	172
Cfr42I	CCGCGG	1 cut(s)	526
Csp6I	GTAC	1 cut(s)	22
CviAII	CATG	2 cut(s)	506, 571
CviQI	GTAC	1 cut(s)	22
DdeI	CTNAG	4 cut(s)	46, 161, 222, 227
DpnI	GATC	2 cut(s)	331, 360
DpnII	GATC	2 cut(s)	329, 358
Eam1104I	CTCTTC	4 cut(s)	219, 254, 266, 424
EarI	CTCTTC	4 cut(s)	219, 254, 266, 424
EciI	GGCGGA	1 cut(s)	545
Ecl136II	GAGCTC	1 cut(s)	43
Eco24I	GRGCYC	2 cut(s)	45, 327
Eco31I	GGTCTC	1 cut(s)	466
Eco47I	GGWCC	1 cut(s)	172
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	1 cut(s)	290
Eco88I	CYCGRG	1 cut(s)	306
EcoICRI	GAGCTC	1 cut(s)	43
EcoT38I	GRGCYC	2 cut(s)	45, 327
FaeI	CATG	2 cut(s)	509, 574
FaiI	YATR	4 cut(s)	170, 507, 572, 584
FatI	CATG	2 cut(s)	505, 570
FauI	CCCGC	1 cut(s)	518
FbaI	TGATCA	1 cut(s)	358
FblI	GTMKAC	1 cut(s)	404
Fnu4HI	GCNGC	4 cut(s)	75, 194, 210, 303
FokI	GGATG	5 cut(s)	101, 108, 158, 323, 545
FriOI	GRGCYC	2 cut(s)	45, 327
Fsp4HI	GCNGC	4 cut(s)	75, 194, 210, 303
FspBI	CTAG	1 cut(s)	670
GlaI	GCGC	2 cut(s)	29, 668
GluI	GCNGC	4 cut(s)	75, 194, 210, 303
HaeII	RGCGCY	1 cut(s)	31
HapII	CCGG	1 cut(s)	327
HhaI	GCGC	2 cut(s)	30, 669
Hin1II	CATG	2 cut(s)	509, 574
Hin6I	GCGC	2 cut(s)	28, 667
HinP1I	GCGC	2 cut(s)	28, 667
HincII	GTYRAC	1 cut(s)	405
HindII	GTYRAC	1 cut(s)	405
HinfI	GANTC	3 cut(s)	109, 338, 414
HpaII	CCGG	1 cut(s)	327
HphI	GGTGA	1 cut(s)	276
Hpy166II	GTNNAC	2 cut(s)	69, 405
Hpy188I	TCNGA	2 cut(s)	114, 620
Hpy188III	TCNNGA	2 cut(s)	292, 308
Hpy8I	GTNNAC	2 cut(s)	69, 405
Hpy99I	CGWCG	1 cut(s)	409
HpyAV	CCTTC	2 cut(s)	199, 550
HpyCH4III	ACNGT	1 cut(s)	318
HpyCH4V	TGCA	6 cut(s)	69, 122, 149, 193, 481, 635
HpyF10VI	GCNNNNNNNGC	3 cut(s)	36, 80, 218
HpyF3I	CTNAG	4 cut(s)	46, 161, 222, 227
Hsp92II	CATG	2 cut(s)	509, 574
HspAI	GCGC	2 cut(s)	28, 667
Ksp22I	TGATCA	1 cut(s)	358
KspI	CCGCGG	1 cut(s)	526
Kzo9I	GATC	2 cut(s)	329, 358
LguI	GCTCTTC	3 cut(s)	219, 254, 424
LmnI	GCTCC	3 cut(s)	322, 451, 614
LpnPI	CCDG	7 cut(s)	120, 300, 340, 376, 425, 506, 574
Lsp1109I	GCAGC	4 cut(s)	61, 205, 221, 314
LweI	GCATC	2 cut(s)	136, 290
MaeI	CTAG	1 cut(s)	670
MaeIII	GTNAC	1 cut(s)	495
MalI	GATC	2 cut(s)	331, 360
MboI	GATC	2 cut(s)	329, 358
MboII	GAAGA	5 cut(s)	206, 271, 283, 381, 441
MhlI	GDGCHC	3 cut(s)	45, 71, 327
MluCI	AATT	3 cut(s)	117, 253, 295
MmeI	TCCRAC	1 cut(s)	300
MseI	TTAA	2 cut(s)	395, 657
MslI	CAYNNNNRTG	1 cut(s)	83
MspA1I	CMGCKG	1 cut(s)	525
MspCI	CTTAAG	1 cut(s)	656
MspI	CCGG	1 cut(s)	327
MspR9I	CCNGG	1 cut(s)	327
MvnI	CGCG	2 cut(s)	525, 667
MwoI	GCNNNNNNNGC	3 cut(s)	36, 80, 218
NciI	CCSGG	1 cut(s)	327
NdeII	GATC	2 cut(s)	329, 358
NlaIII	CATG	2 cut(s)	509, 574
NlaIV	GGNNCC	2 cut(s)	324, 610
NmuCI	GTSAC	1 cut(s)	495
NspI	RCATGY	1 cut(s)	509
OliI	CACNNNNGTG	1 cut(s)	83
PciSI	GCTCTTC	3 cut(s)	219, 254, 424
PfeI	GAWTC	3 cut(s)	109, 338, 414
PkrI	GCNGC	4 cut(s)	76, 195, 211, 304
Psp124BI	GAGCTC	1 cut(s)	45
PspN4I	GGNNCC	2 cut(s)	324, 610
PspPI	GGNCC	1 cut(s)	172
RsaI	GTAC	1 cut(s)	23
RsaNI	GTAC	1 cut(s)	22
RseI	CAYNNNNRTG	1 cut(s)	83
SacI	GAGCTC	1 cut(s)	45
SacII	CCGCGG	1 cut(s)	526
SalI	GTCGAC	1 cut(s)	403
SapI	GCTCTTC	3 cut(s)	219, 254, 424
SaqAI	TTAA	2 cut(s)	395, 657
SatI	GCNGC	4 cut(s)	75, 194, 210, 303
Sau3AI	GATC	2 cut(s)	329, 358
Sau96I	GGNCC	1 cut(s)	172
ScrFI	CCNGG	1 cut(s)	327
SduI	GDGCHC	3 cut(s)	45, 71, 327
SfaNI	GCATC	2 cut(s)	136, 290
Sfr303I	CCGCGG	1 cut(s)	526
SgrBI	CCGCGG	1 cut(s)	526
SinI	GGWCC	1 cut(s)	172
SmiMI	CAYNNNNRTG	1 cut(s)	83
SmlI	CTYRAG	3 cut(s)	58, 176, 656
SmoI	CTYRAG	3 cut(s)	58, 176, 656
Sse9I	AATT	3 cut(s)	117, 253, 295
SsiI	CCGC	5 cut(s)	81, 523, 525, 530, 567
SspMI	CTAG	1 cut(s)	670
SstI	GAGCTC	1 cut(s)	45
StyD4I	CCNGG	1 cut(s)	325
TaaI	ACNGT	1 cut(s)	318
TaqI	TCGA	2 cut(s)	353, 404
TasI	AATT	3 cut(s)	117, 253, 295
TfiI	GAWTC	3 cut(s)	109, 338, 414
Tru1I	TTAA	2 cut(s)	395, 657
Tru9I	TTAA	2 cut(s)	395, 657
TseFI	GTSAC	1 cut(s)	495
TseI	GCWGC	4 cut(s)	74, 193, 209, 302
Tsp45I	GTSAC	1 cut(s)	495
TspDTI	ATGAA	3 cut(s)	327, 549, 599
TspGWI	ACGGA	1 cut(s)	422
Vha464I	CTTAAG	1 cut(s)	656
VneI	GTGCAC	1 cut(s)	67
VpaK11BI	GGWCC	1 cut(s)	172
XapI	RAATTY	1 cut(s)	295
XceI	RCATGY	1 cut(s)	509
XmiI	GTMKAC	1 cut(s)	404
XspI	CTAG	1 cut(s)	670

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.