Rw0G007530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00270
OG0001487
Physical Location & Seq
Forward (+)
315673 .. 317070
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007530.1

Sequence Viewer

Length: 447 bp
ATGAGTGAAAGCTCGTGGAGTTGCCTCGGTGCCCAGGAGTACAAAACCACCGTCCAACAAGAGTTCGAGCAAGACTTCAGAAATGCGCACCCAAATAATTACAATAGCTTCAGAGTTGAGTGTGAGGCTATGAACTCGTTAGCGCTCACCATGGACTTGAAGGTTACTGCGGAATCTAATTCCAACGGCATTGCGGAGTTGAAGACATGTGGGGGGTGGTTCGTTGACCCAAAGTACAGATGCCTAGTATATTTGGAAGACGGTACCTGGAATGGGTTGTTCCAGCCATTGCTGCCGGAGTACGACTGTGTCGAAAAGAACCACCACTGCTGGTGGAAGCTGCATGACGGGTATTTACTTCGCCACTCGCCCAAGCGAAACGGCTGGCTTCGTCAAAATTATGGCCTCGGCTGGGTTCCGCAACCCACAGCTGAAGACATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

17.22

Weight (kDa)

5.49

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 87
Acc65I GGTACC 1 cut(s) 263
AccB1I GGYRCC 2 cut(s) 29, 263
AciI CCGC 3 cut(s) 170, 194, 419
AcuI CTGAAG 2 cut(s) 61, 94
AfaI GTAC 4 cut(s) 41, 236, 265, 302
AfeI AGCGCT 1 cut(s) 144
AfiI CCNNNNNNNGG 2 cut(s) 273, 412
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 2 cut(s) 160, 202
AjnI CCWGG 2 cut(s) 33, 266
AluBI AGCT 4 cut(s) 12, 108, 340, 431
AluI AGCT 4 cut(s) 12, 108, 340, 431
Aor51HI AGCGCT 1 cut(s) 144
AoxI GGCC 1 cut(s) 403
ApeKI GCWGC 2 cut(s) 292, 340
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
Asp718I GGTACC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 88, 145
AsuHPI GGTGA 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 34
BanI GGYRCC 2 cut(s) 29, 263
BauI CACGAG 1 cut(s) 13
BbsI GAAGAC 3 cut(s) 209, 264, 441
BbvI GCAGC 2 cut(s) 279, 327
BceAI ACGGC 2 cut(s) 202, 397
BciT130I CCWGG 2 cut(s) 35, 268
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 146
BisI GCNGC 2 cut(s) 293, 341
BlsI GCNGC 2 cut(s) 294, 342
Bme1390I CCNGG 2 cut(s) 35, 268
BmiI GGNNCC 3 cut(s) 31, 265, 417
BmrFI CCNGG 2 cut(s) 35, 268
BmsI GCATC 1 cut(s) 230
BpiI GAAGAC 3 cut(s) 209, 264, 441
BsaJI CCNNGG 4 cut(s) 25, 33, 150, 406
Bsc4I CCNNNNNNNGG 2 cut(s) 273, 412
Bse3DI GCAATG 2 cut(s) 189, 287
BseBI CCWGG 2 cut(s) 35, 268
BseDI CCNNGG 4 cut(s) 25, 33, 150, 406
BseLI CCNNNNNNNGG 2 cut(s) 273, 412
BseMI GCAATG 2 cut(s) 189, 287
BseSI GKGCMC 1 cut(s) 34
BseXI GCAGC 2 cut(s) 279, 327
BseYI CCCAGC 1 cut(s) 411
BshFI GGCC 1 cut(s) 405
BshNI GGYRCC 2 cut(s) 29, 263
BsiSI CCGG 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 273, 412
BsnI GGCC 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 34
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 3 cut(s) 170, 194, 419
BspANI GGCC 1 cut(s) 405
BspLI GGNNCC 3 cut(s) 31, 265, 417
BspT107I GGYRCC 2 cut(s) 29, 263
BsrDI GCAATG 2 cut(s) 189, 287
BssECI CCNNGG 4 cut(s) 25, 33, 150, 406
BssSI CACGAG 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 13
Bst2UI CCWGG 2 cut(s) 35, 268
Bst4CI ACNGT 3 cut(s) 52, 263, 308
BstC8I GCNNGC 1 cut(s) 386
BstDSI CCRYGG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 2 cut(s) 88, 145
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstNI CCWGG 2 cut(s) 35, 268
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 2 cut(s) 33, 266
BstSLI GKGCMC 1 cut(s) 34
BstV1I GCAGC 2 cut(s) 279, 327
BstV2I GAAGAC 3 cut(s) 209, 264, 441
BsuRI GGCC 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 386
CfoI GCGC 2 cut(s) 88, 145
Csp6I GTAC 4 cut(s) 40, 235, 264, 301
CviAII CATG 3 cut(s) 151, 207, 344
CviQI GTAC 4 cut(s) 40, 235, 264, 301
Eco130I CCWWGG 1 cut(s) 150
Eco47III AGCGCT 1 cut(s) 144
Eco57I CTGAAG 2 cut(s) 61, 94
EcoRII CCWGG 2 cut(s) 33, 266
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 154, 210, 347
FaiI YATR 7 cut(s) 131, 152, 208, 250, 345, 402, 440
FatI CATG 3 cut(s) 150, 206, 343
Fnu4HI GCNGC 2 cut(s) 293, 341
Fsp4HI GCNGC 2 cut(s) 293, 341
FspAI RTGCGCAY 1 cut(s) 87
FspBI CTAG 1 cut(s) 245
FspI TGCGCA 1 cut(s) 87
GlaI GCGC 2 cut(s) 87, 144
GluI GCNGC 2 cut(s) 293, 341
GsaI CCCAGC 1 cut(s) 415
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 1 cut(s) 405
HapII CCGG 1 cut(s) 296
HhaI GCGC 2 cut(s) 88, 145
Hin1II CATG 3 cut(s) 154, 210, 347
Hin6I GCGC 2 cut(s) 86, 143
HinP1I GCGC 2 cut(s) 86, 143
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 1 cut(s) 173
HpaII CCGG 1 cut(s) 296
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 2 cut(s) 80, 113
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 3 cut(s) 52, 263, 308
HpyCH4V TGCA 1 cut(s) 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
Hsp92II CATG 3 cut(s) 154, 210, 347
HspAI GCGC 2 cut(s) 86, 143
KpnI GGTACC 1 cut(s) 267
LpnPI CCDG 9 cut(s) 20, 47, 253, 280, 296, 309, 316, 370, 397
Lsp1109I GCAGC 2 cut(s) 279, 327
LweI GCATC 1 cut(s) 230
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 163
MboII GAAGA 3 cut(s) 214, 269, 446
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 3 cut(s) 97, 178, 397
MmeI TCCRAC 2 cut(s) 79, 207
MnlI CCTC 3 cut(s) 35, 118, 416
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 35, 268
MvaI CCWGG 2 cut(s) 35, 268
MwoI GCNNNNNNNGC 1 cut(s) 292
NcoI CCATGG 1 cut(s) 150
NlaIII CATG 3 cut(s) 154, 210, 347
NlaIV GGNNCC 3 cut(s) 31, 265, 417
NmeAIII GCCGAG 1 cut(s) 387
NsbI TGCGCA 1 cut(s) 87
NspI RCATGY 1 cut(s) 210
PciI ACATGT 1 cut(s) 206
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PfeI GAWTC 1 cut(s) 173
PflFI GACNNNGTC 1 cut(s) 308
PkrI GCNGC 2 cut(s) 294, 342
PscI ACATGT 1 cut(s) 206
Psp6I CCWGG 2 cut(s) 33, 266
PspFI CCCAGC 1 cut(s) 411
PspGI CCWGG 2 cut(s) 33, 266
PspN4I GGNNCC 3 cut(s) 31, 265, 417
PsyI GACNNNGTC 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 4 cut(s) 41, 236, 265, 302
RsaNI GTAC 4 cut(s) 40, 235, 264, 301
SatI GCNGC 2 cut(s) 293, 341
ScrFI CCNGG 2 cut(s) 35, 268
SduI GDGCHC 1 cut(s) 34
SetI ASST 6 cut(s) 14, 110, 165, 269, 342, 433
SfaNI GCATC 1 cut(s) 230
Sse9I AATT 3 cut(s) 97, 178, 397
SsiI CCGC 3 cut(s) 170, 194, 419
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 2 cut(s) 33, 266
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 3 cut(s) 52, 263, 308
TaqI TCGA 2 cut(s) 66, 312
TasI AATT 3 cut(s) 97, 178, 397
TatI WGTACW 2 cut(s) 39, 234
TfiI GAWTC 1 cut(s) 173
TscAI CASTG 1 cut(s) 332
TseI GCWGC 2 cut(s) 292, 340
TspDTI ATGAA 1 cut(s) 146
TspRI CASTG 1 cut(s) 332
Tth111I GACNNNGTC 1 cut(s) 308
XceI RCATGY 1 cut(s) 210
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.