FvH4_5g06620

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000761
Physical Location & Seq
Forward (+)
3906722 .. 3909352
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06620.t1

Sequence Viewer

Length: 1347 bp
ATGGGCGGGATAACTTCAAGAAGTAGTTCGTCAAGACAGTCTTCTTTTGCGTCTAGATCATCTTCCTGGAATCATCAGAATTATGAACAGGCGCCGTATGCACAACCTTATGCACCTCCAAGTCATGACGGTGGGCAACAGCAGCATTATGCACCTGCATCTCAAAGCCATAGTGGTGGTGCTTGGGGATCAGAGTCTAAGAAGAGGTTGGAAAGAAAGTACTCAAAGATAGATGATAATTACAACAGCCTTGACCAGGTCACAGATGCATTGGCCCGCGCTGGTCTCGAGTCTTCTAATCTTATTGTTGGGATTGATTTCACTAAGAGCAATGAGTGGACAGGTGCTAGGTCATTTCACAGGAAAAGCTTGCATCACATTGGAGATGAGCAAAATCCCTATGAACAAGCAATAGCTATTATCGGGAAAACATTATCTTCTTTTGATGAGGATAACATGATTCCTTGCTATGGATTTGGAGATGCATCAACCCATGACCAAGAAGTTTTCAGTTTCTACCCTGATGAGGACACCTATTGTAACGGATTTGAAGAAGTTTTGAGCCGATATAGAGAATTAGTTCCTCAGCTACGACTTGCAGGACCCACATCATTTGCCCCTATCATTGAAATGGCCATGACCATAGTTGAGCAAAGTGGTGGTCAGTATCATGTATTAGTGATCATAGCTGATGGGCAGGTGACTAGAAGTGTTGAGACTGGTCGTGGCCAATTCAGCCCACAGGAAAAGAAAACTGTTGATGCAATTGTTAAGGCAAGTGAGTATCCCCTGTCAATTATAGTAGTTGGAGTTGGAGACGGACCATGGGATATGATGAAAGAATTTGATGATAACATTCCTGCACGAGCCTTTGATAACTTCCAGTTTGTGAATTTTACAGACATTATGTCAAAGAATACAGACCGCTCAAGAAAAGAAGCAGAATTTGCTCTTGCAGCCCTTATGGAAATACCCTCTCAGTATAAAGCGACACTAGAGCTCGGTATATTGGGTGTTACAAGAGGGAAGGCTGTAGATAGGATCCCGCTTCCCCCTCCTCATTATGGTTCGGCTTCTTTTGGCACCTCAAAAACTTCACGTGCAAATAGTTTTCGTCCAACTGCACCTTCAAGGCCACAACATCAGGATGTTAGCCATAGCAGAGCGCCTCCTGCTGGTTCTTCTTCCGCTTCTGCTTCTGACAATCATGTATGTCCCATTTGCTTAACCGATCCAAAAAATATGGCATTTGGTTGTGGACACCAGACATGCTGCGACTGTGGACAAGATCTTCAGTTATGCCCGATTTGCAGAAGCACCATCGAAACCAGGATTAAGCTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

449

Amino Acids

49.25

Weight (kDa)

5.79

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Copine PF07002 123 - 336 1.6e-74 Copine
zf-C3HC4_3 PF13920 402 - 443 2.4e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 163, 688
Acc36I ACCTGC 2 cut(s) 163, 688
AccB1I GGYRCC 2 cut(s) 91, 1082
AccBSI CCGCTC 1 cut(s) 927
AccII CGCG 1 cut(s) 279
AciI CCGC 5 cut(s) 6, 277, 925, 1046, 1188
AclWI GGATC 4 cut(s) 196, 1036, 1049, 1226
AcoI YGGCCR 2 cut(s) 633, 727
AcsI RAATTY 3 cut(s) 842, 892, 944
AcuI CTGAAG 1 cut(s) 1277
AcvI CACGTG 1 cut(s) 1100
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 5 cut(s) 256, 470, 526, 1064, 1175
AgsI TTSAA 4 cut(s) 18, 551, 629, 1131
AhdI GACNNNNNGTC 1 cut(s) 907
AjnI CCWGG 3 cut(s) 65, 255, 1328
AjuI GAANNNNNNNTTGG 2 cut(s) 1111, 1143
AluBI AGCT 6 cut(s) 369, 416, 589, 689, 1000, 1339
AluI AGCT 6 cut(s) 369, 416, 589, 689, 1000, 1339
Alw21I GWGCWC 1 cut(s) 1002
Alw26I GTCTC 3 cut(s) 290, 710, 810
AlwI GGATC 4 cut(s) 196, 1036, 1049, 1226
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 4 cut(s) 273, 633, 727, 1133
ApeKI GCWGC 3 cut(s) 142, 956, 1272
ApoI RAATTY 3 cut(s) 842, 892, 944
ArsI GACNNNNNNTTYG 2 cut(s) 646, 678
Asp700I GAANNNNTTC 2 cut(s) 25, 579
AspLEI GCGC 3 cut(s) 94, 281, 1168
AspS9I GGNCC 3 cut(s) 274, 602, 821
AsuHPI GGTGA 1 cut(s) 712
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 2 cut(s) 602, 821
BalI TGGCCA 2 cut(s) 635, 729
BamHI GGATCC 1 cut(s) 1041
BanI GGYRCC 2 cut(s) 91, 1082
BanII GRGCYC 1 cut(s) 1002
BauI CACGAG 1 cut(s) 864
BbrPI CACGTG 1 cut(s) 1100
BbsI GAAGAC 2 cut(s) 33, 285
Bbv12I GWGCWC 1 cut(s) 1002
BbvCI CCTCAGC 1 cut(s) 585
BbvI GCAGC 3 cut(s) 154, 968, 1259
BccI CCATC 2 cut(s) 686, 1328
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 3 cut(s) 67, 257, 1330
BciVI GTATCC 1 cut(s) 795
BclI TGATCA 1 cut(s) 681
BcoDI GTCTC 3 cut(s) 290, 710, 810
BfaI CTAG 4 cut(s) 54, 348, 705, 995
BfmI CTRYAG 1 cut(s) 1032
BfoI RGCGCY 2 cut(s) 95, 1169
BfuAI ACCTGC 2 cut(s) 163, 688
BfuI GTATCC 1 cut(s) 795
BglII AGATCT 1 cut(s) 1288
BisI GCNGC 3 cut(s) 143, 957, 1273
BlsI GCNGC 3 cut(s) 144, 958, 1274
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 3 cut(s) 67, 257, 1330
Bme18I GGWCC 2 cut(s) 602, 821
BmeRI GACNNNNNGTC 1 cut(s) 907
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 274, 602, 821
BmiI GGNNCC 4 cut(s) 93, 604, 1043, 1084
BmrFI CCNGG 3 cut(s) 67, 257, 1330
BmsI GCATC 6 cut(s) 167, 256, 382, 472, 494, 751
BpiI GAAGAC 2 cut(s) 33, 285
Bpu10I CCTNAGC 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 913
BsaAI YACGTR 1 cut(s) 1100
BsaHI GRCGYC 1 cut(s) 92
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 824
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 470, 526, 1064, 1175
Bse1I ACTGG 2 cut(s) 724, 883
Bse3DI GCAATG 1 cut(s) 337
BseBI CCWGG 3 cut(s) 67, 257, 1330
BseDI CCNNGG 1 cut(s) 824
BseGI GGATG 1 cut(s) 1153
BseLI CCNNNNNNNGG 5 cut(s) 256, 470, 526, 1064, 1175
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 599, 992
BseNI ACTGG 2 cut(s) 724, 883
BseRI GAGGAG 1 cut(s) 1047
BseXI GCAGC 3 cut(s) 154, 968, 1259
BsgI GTGCAG 2 cut(s) 846, 1107
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 4 cut(s) 275, 635, 729, 1135
BshNI GGYRCC 2 cut(s) 91, 1082
BsiHKAI GWGCWC 1 cut(s) 1002
BsiHKCI CYCGRG 1 cut(s) 287
BslFI GGGAC 1 cut(s) 1200
BslI CCNNNNNNNGG 5 cut(s) 256, 470, 526, 1064, 1175
BsmAI GTCTC 3 cut(s) 290, 710, 810
BsmBI CGTCTC 1 cut(s) 810
BsmFI GGGAC 1 cut(s) 1200
BsnI GGCC 4 cut(s) 275, 635, 729, 1135
Bso31I GGTCTC 1 cut(s) 290
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 1002
Bsp143I GATC 6 cut(s) 56, 188, 681, 1041, 1231, 1288
Bsp19I CCATGG 1 cut(s) 824
BspACI CCGC 5 cut(s) 6, 277, 925, 1046, 1188
BspANI GGCC 4 cut(s) 275, 635, 729, 1135
BspCNI CTCAG 2 cut(s) 598, 991
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 4 cut(s) 93, 604, 1043, 1084
BspMI ACCTGC 2 cut(s) 163, 688
BspPI GGATC 4 cut(s) 196, 1036, 1049, 1226
BspT107I GGYRCC 2 cut(s) 91, 1082
BspTNI GGTCTC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 927
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 2 cut(s) 724, 883
BssECI CCNNGG 1 cut(s) 824
BssMI GATC 6 cut(s) 56, 188, 681, 1041, 1231, 1288
BssNI GRCGYC 1 cut(s) 92
BssSI CACGAG 1 cut(s) 864
BssT1I CCWWGG 1 cut(s) 824
Bst2BI CACGAG 1 cut(s) 864
Bst2UI CCWGG 3 cut(s) 67, 257, 1330
Bst4CI ACNGT 4 cut(s) 39, 131, 757, 1280
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 947
BstBAI YACGTR 1 cut(s) 1100
BstC8I GCNNGC 2 cut(s) 277, 371
BstDEI CTNAG 4 cut(s) 198, 324, 585, 978
BstDSI CCRYGG 1 cut(s) 824
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 1 cut(s) 1153
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 2 cut(s) 95, 1169
BstHHI GCGC 3 cut(s) 94, 281, 1168
BstKTI GATC 6 cut(s) 59, 191, 684, 1044, 1234, 1291
BstMAI GTCTC 3 cut(s) 290, 710, 810
BstMBI GATC 6 cut(s) 56, 188, 681, 1041, 1231, 1288
BstMWI GCNNNNNNNGC 7 cut(s) 98, 142, 735, 947, 956, 1172, 1308
BstNI CCWGG 3 cut(s) 67, 257, 1330
BstNSI RCATGY 1 cut(s) 1272
BstSCI CCNGG 3 cut(s) 65, 255, 1328
BstSFI CTRYAG 1 cut(s) 1032
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 3 cut(s) 154, 968, 1259
BstV2I GAAGAC 2 cut(s) 33, 285
BstX2I RGATCY 2 cut(s) 1041, 1288
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 2 cut(s) 1041, 1288
BsuI GTATCC 1 cut(s) 795
BsuRI GGCC 4 cut(s) 275, 635, 729, 1135
BtgI CCRYGG 1 cut(s) 824
BtsCI GGATG 1 cut(s) 1153
BveI ACCTGC 2 cut(s) 163, 688
Cac8I GCNNGC 2 cut(s) 277, 371
CciI TCATGA 1 cut(s) 124
CfoI GCGC 3 cut(s) 94, 281, 1168
Cfr13I GGNCC 3 cut(s) 274, 602, 821
CseI GACGC 1 cut(s) 39
CsiI ACCWGGT 1 cut(s) 255
Csp6I GTAC 1 cut(s) 220
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviAII CATG 8 cut(s) 125, 457, 494, 637, 671, 825, 1208, 1269
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 4 cut(s) 198, 324, 585, 978
DinI GGCGCC 1 cut(s) 93
DpnI GATC 6 cut(s) 58, 190, 683, 1043, 1233, 1290
DpnII GATC 6 cut(s) 56, 188, 681, 1041, 1231, 1288
DriI GACNNNNNGTC 1 cut(s) 907
EaeI YGGCCR 2 cut(s) 633, 727
Eam1104I CTCTTC 1 cut(s) 197
Eam1105I GACNNNNNGTC 1 cut(s) 907
EarI CTCTTC 1 cut(s) 197
Ecl136II GAGCTC 1 cut(s) 1000
Eco130I CCWWGG 1 cut(s) 824
Eco24I GRGCYC 1 cut(s) 1002
Eco31I GGTCTC 1 cut(s) 290
Eco47I GGWCC 2 cut(s) 602, 821
Eco53kI GAGCTC 1 cut(s) 1000
Eco57I CTGAAG 1 cut(s) 1277
Eco72I CACGTG 1 cut(s) 1100
Eco88I CYCGRG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 1000
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 3 cut(s) 65, 255, 1328
EcoT14I CCWWGG 1 cut(s) 824
EcoT22I ATGCAT 2 cut(s) 271, 487
EcoT38I GRGCYC 1 cut(s) 1002
EgeI GGCGCC 1 cut(s) 93
EheI GGCGCC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 824
Esp3I CGTCTC 1 cut(s) 810
FaeI CATG 8 cut(s) 128, 460, 497, 640, 674, 828, 1211, 1272
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FaqI GGGAC 1 cut(s) 1200
FatI CATG 8 cut(s) 124, 456, 493, 636, 670, 824, 1207, 1268
FauI CCCGC 2 cut(s) 284, 1053
FbaI TGATCA 1 cut(s) 681
Fnu4HI GCNGC 3 cut(s) 143, 957, 1273
FokI GGATG 1 cut(s) 1160
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 3 cut(s) 143, 957, 1273
FspBI CTAG 4 cut(s) 54, 348, 705, 995
GlaI GCGC 3 cut(s) 93, 280, 1167
GluI GCNGC 3 cut(s) 143, 957, 1273
HaeII RGCGCY 2 cut(s) 95, 1169
HaeIII GGCC 4 cut(s) 275, 635, 729, 1135
HgaI GACGC 1 cut(s) 39
HhaI GCGC 3 cut(s) 94, 281, 1168
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 8 cut(s) 128, 460, 497, 640, 674, 828, 1211, 1272
Hin6I GCGC 3 cut(s) 92, 279, 1166
HinP1I GCGC 3 cut(s) 92, 279, 1166
HindIII AAGCTT 2 cut(s) 367, 1337
HinfI GANTC 4 cut(s) 70, 194, 290, 460
HphI GGTGA 1 cut(s) 712
Hpy166II GTNNAC 3 cut(s) 339, 1259, 1283
Hpy188I TCNGA 3 cut(s) 78, 193, 1201
Hpy188III TCNNGA 8 cut(s) 18, 33, 54, 125, 287, 424, 930, 1145
Hpy8I GTNNAC 3 cut(s) 339, 1259, 1283
HpyAV CCTTC 2 cut(s) 1021, 1137
HpyCH4III ACNGT 4 cut(s) 39, 131, 757, 1280
HpyCH4IV ACGT 1 cut(s) 1099
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 142, 735, 947, 956, 1172, 1308
HpyF3I CTNAG 4 cut(s) 198, 324, 585, 978
HpySE526I ACGT 1 cut(s) 1099
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 8 cut(s) 128, 460, 497, 640, 674, 828, 1211, 1272
HspAI GCGC 3 cut(s) 92, 279, 1166
KasI GGCGCC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 6 cut(s) 56, 188, 681, 1041, 1231, 1288
Lsp1109I GCAGC 3 cut(s) 154, 968, 1259
LweI GCATC 6 cut(s) 167, 256, 382, 472, 494, 751
MabI ACCWGGT 1 cut(s) 255
MaeI CTAG 4 cut(s) 54, 348, 705, 995
MaeII ACGT 1 cut(s) 1099
MaeIII GTNAC 4 cut(s) 259, 539, 700, 1015
MalI GATC 6 cut(s) 58, 190, 683, 1043, 1233, 1290
MbiI CCGCTC 1 cut(s) 927
MboI GATC 6 cut(s) 56, 188, 681, 1041, 1231, 1288
MboII GAAGA 9 cut(s) 33, 54, 214, 285, 429, 563, 1173, 1176, 1283
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 2 cut(s) 1041, 1288
MhlI GDGCHC 1 cut(s) 1002
MlsI TGGCCA 2 cut(s) 635, 729
MluCI AATT 9 cut(s) 79, 238, 575, 731, 765, 795, 842, 892, 944
MluNI TGGCCA 2 cut(s) 635, 729
Mly113I GGCGCC 1 cut(s) 92
MlyI GAGTC 2 cut(s) 203, 299
MmeI TCCRAC 4 cut(s) 189, 787, 793, 1142
Mox20I TGGCCA 2 cut(s) 635, 729
Mph1103I ATGCAT 2 cut(s) 271, 487
MroXI GAANNNNTTC 2 cut(s) 25, 579
MscI TGGCCA 2 cut(s) 635, 729
MseI TTAA 4 cut(s) 771, 1226, 1335, 1345
MslI CAYNNNNRTG 4 cut(s) 129, 174, 629, 1146
Msp20I TGGCCA 2 cut(s) 635, 729
MspR9I CCNGG 3 cut(s) 67, 257, 1330
MunI CAATTG 1 cut(s) 765
MvaI CCWGG 3 cut(s) 67, 257, 1330
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 7 cut(s) 98, 142, 735, 947, 956, 1172, 1308
NarI GGCGCC 1 cut(s) 92
NcoI CCATGG 1 cut(s) 824
NdeII GATC 6 cut(s) 56, 188, 681, 1041, 1231, 1288
NlaIII CATG 8 cut(s) 128, 460, 497, 640, 674, 828, 1211, 1272
NlaIV GGNNCC 4 cut(s) 93, 604, 1043, 1084
NmuCI GTSAC 2 cut(s) 259, 700
NsiI ATGCAT 2 cut(s) 271, 487
NspI RCATGY 1 cut(s) 1272
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 1 cut(s) 124
PaqCI CACCTGC 2 cut(s) 163, 688
PdmI GAANNNNTTC 2 cut(s) 25, 579
PfeI GAWTC 2 cut(s) 70, 460
PflFI GACNNNGTC 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 65
PkrI GCNGC 3 cut(s) 144, 958, 1274
PleI GAGTC 2 cut(s) 202, 298
PluTI GGCGCC 1 cut(s) 95
PmaCI CACGTG 1 cut(s) 1100
PmlI CACGTG 1 cut(s) 1100
PpsI GAGTC 2 cut(s) 202, 298
Ppu21I YACGTR 1 cut(s) 1100
PpuMI RGGWCCY 1 cut(s) 602
Psp124BI GAGCTC 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 3 cut(s) 65, 255, 1328
PspCI CACGTG 1 cut(s) 1100
PspGI CCWGG 3 cut(s) 65, 255, 1328
PspN4I GGNNCC 4 cut(s) 93, 604, 1043, 1084
PspPI GGNCC 3 cut(s) 274, 602, 821
PspPPI RGGWCCY 1 cut(s) 602
PsuI RGATCY 2 cut(s) 1041, 1288
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 4 cut(s) 129, 174, 629, 1146
SacI GAGCTC 1 cut(s) 1002
SaqAI TTAA 4 cut(s) 771, 1226, 1335, 1345
SatI GCNGC 3 cut(s) 143, 957, 1273
Sau3AI GATC 6 cut(s) 56, 188, 681, 1041, 1231, 1288
Sau96I GGNCC 3 cut(s) 274, 602, 821
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 2 cut(s) 203, 299
ScrFI CCNGG 3 cut(s) 67, 257, 1330
SduI GDGCHC 1 cut(s) 1002
SexAI ACCWGGT 1 cut(s) 255
SfaNI GCATC 6 cut(s) 167, 256, 382, 472, 494, 751
SfcI CTRYAG 1 cut(s) 1032
SfoI GGCGCC 1 cut(s) 93
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 2 cut(s) 602, 821
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 4 cut(s) 129, 174, 629, 1146
SmlI CTYRAG 2 cut(s) 287, 928
SmoI CTYRAG 2 cut(s) 287, 928
Sse9I AATT 9 cut(s) 79, 238, 575, 731, 765, 795, 842, 892, 944
SsiI CCGC 5 cut(s) 6, 277, 925, 1046, 1188
SspDI GGCGCC 1 cut(s) 91
SspMI CTAG 4 cut(s) 54, 348, 705, 995
SstI GAGCTC 1 cut(s) 1002
StyD4I CCNGG 3 cut(s) 65, 255, 1328
StyI CCWWGG 1 cut(s) 824
TaaI ACNGT 4 cut(s) 39, 131, 757, 1280
TaiI ACGT 1 cut(s) 1102
TaqI TCGA 2 cut(s) 288, 1323
TasI AATT 9 cut(s) 79, 238, 575, 731, 765, 795, 842, 892, 944
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 2 cut(s) 70, 460
Tru1I TTAA 4 cut(s) 771, 1226, 1335, 1345
Tru9I TTAA 4 cut(s) 771, 1226, 1335, 1345
TseFI GTSAC 2 cut(s) 259, 700
TseI GCWGC 3 cut(s) 142, 956, 1272
Tsp45I GTSAC 2 cut(s) 259, 700
TspDTI ATGAA 3 cut(s) 99, 417, 851
TspGWI ACGGA 2 cut(s) 558, 834
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 602, 821
XagI CCTNNNNNAGG 1 cut(s) 254
XapI RAATTY 3 cut(s) 842, 892, 944
XbaI TCTAGA 1 cut(s) 53
XceI RCATGY 1 cut(s) 1272
XhoI CTCGAG 1 cut(s) 287
XmnI GAANNNNTTC 2 cut(s) 25, 579
XspI CTAG 4 cut(s) 54, 348, 705, 995
ZrmI AGTACT 1 cut(s) 221
Zsp2I ATGCAT 2 cut(s) 271, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.