Rorug06G0513100

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000761
Physical Location & Seq
Reverse (-)
62789102 .. 62790081
980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0513100.1

Sequence Viewer

Length: 564 bp
ATGATGAAGCAGCTGGATGAGCTGAAAGATATGATTAACTTGATGATTCTTGATAGTTATGGGTTGGGAGAGAAATCAAATTCGACTTTGCAGTCTAAGACACTGCTAAGGATAATGAAATACCTGGCACCTCCTTCGAAGGACTACACTCTGGCGCTCCCGGCTCACACTGACAAGGGATTGGGCACAATTCTTTGTGATGATCATGTTTCAGGTCTTGAAGTTGAAACAAAGGATGGACAATGGGTCAAGTTGTCTCTATCTCCTCGTTCCTTCGTCTTTCTTGTTGCAGATTCCCTCATGGCATGGAGCAATGGAAGAATGCATTCTGTGAAGCATCGAGTGATGATGCGTGGAGAAAAAGAACGATATTCTTTAGGAGTATTCGCAGTTCCATTGGAGGGTTCCATCATTAAGGCACAAAAGGAGCTAGTAGATGAAGAACATCCCCAAATTCTTAAAGATTTTGATTATACAGATTTTAGCAAGTTCTTCGCTTCGGAAAAAGGAAGGGGTATTGACCCAGAAAAGCAAGTTTTCGCATATGCTGGAATTAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

187

Amino Acids

21.08

Weight (kDa)

6.21

Isoelectric Point (pI)

30.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 38 - 130 5.3e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 91
AccB1I GGYRCC 1 cut(s) 127
AcsI RAATTY 2 cut(s) 79, 453
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 2 cut(s) 221, 227
AhdI GACNNNNNGTC 1 cut(s) 245
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 3 cut(s) 13, 22, 430
AluI AGCT 3 cut(s) 13, 22, 430
Alw26I GTCTC 1 cut(s) 261
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 79, 453
Asp700I GAANNNNTTC 1 cut(s) 325
AspLEI GCGC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 161
AsuII TTCGAA 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 188
BanI GGYRCC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 22
BccI CCATC 2 cut(s) 230, 416
BcgI CGANNNNNNTGC 4 cut(s) 117, 151, 475, 509
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 1 cut(s) 202
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 261
BfaI CTAG 1 cut(s) 431
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 1 cut(s) 11
BlsI GCNGC 1 cut(s) 12
Bme1390I CCNGG 2 cut(s) 125, 161
BmeRI GACNNNNNGTC 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 129, 406
BmrFI CCNGG 2 cut(s) 125, 161
BmsI GCATC 2 cut(s) 339, 346
Bpu10I CCTNAGC 1 cut(s) 107
Bpu14I TTCGAA 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 161
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 125
BseGI GGATG 3 cut(s) 22, 241, 445
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 319
BseRI GAGGAG 1 cut(s) 255
BseSI GKGCMC 1 cut(s) 188
BseXI GCAGC 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 127
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 261
BsmI GAATGC 2 cut(s) 325, 327
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 1 cut(s) 202
BspLI GGNNCC 2 cut(s) 129, 406
BspT104I TTCGAA 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 319
BssMI GATC 1 cut(s) 202
Bst2UI CCWGG 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 96, 107
BstF5I GGATG 3 cut(s) 22, 241, 445
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 2 cut(s) 19, 161
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 123, 159
BstSLI GKGCMC 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 22
BtsCI GGATG 3 cut(s) 22, 241, 445
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 2 cut(s) 101, 168
CfoI GCGC 1 cut(s) 157
CviAII CATG 3 cut(s) 206, 301, 306
CviJI RGCY 4 cut(s) 13, 22, 164, 430
CviKI_1 RGCY 4 cut(s) 13, 22, 164, 430
DdeI CTNAG 2 cut(s) 96, 107
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
DrdI GACNNNNNNGTC 1 cut(s) 91
DriI GACNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 91
Eam1105I GACNNNNNGTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 123
EcoT22I ATGCAT 1 cut(s) 327
FaeI CATG 3 cut(s) 209, 304, 309
FaiI YATR 8 cut(s) 32, 60, 207, 302, 307, 474, 544, 546
FatI CATG 3 cut(s) 205, 300, 305
FauNDI CATATG 1 cut(s) 544
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 1 cut(s) 11
FokI GGATG 3 cut(s) 29, 248, 432
Fsp4HI GCNGC 1 cut(s) 11
FspBI CTAG 1 cut(s) 431
GlaI GCGC 1 cut(s) 156
GluI GCNGC 1 cut(s) 11
HaeII RGCGCY 1 cut(s) 158
HapII CCGG 1 cut(s) 161
HhaI GCGC 1 cut(s) 157
Hin1II CATG 3 cut(s) 209, 304, 309
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 2 cut(s) 46, 293
HpaII CCGG 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 502
Hpy188III TCNNGA 2 cut(s) 50, 218
HpyAV CCTTC 4 cut(s) 133, 144, 283, 504
HpyCH4V TGCA 3 cut(s) 91, 290, 325
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 161
HpyF3I CTNAG 2 cut(s) 96, 107
Hsp92II CATG 3 cut(s) 209, 304, 309
HspAI GCGC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 3 cut(s) 162, 309, 427
LpnPI CCDG 7 cut(s) 110, 137, 137, 174, 198, 534, 537
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 2 cut(s) 339, 346
MaeI CTAG 1 cut(s) 431
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 3 cut(s) 330, 452, 484
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 4 cut(s) 79, 189, 453, 552
MnlI CCTC 4 cut(s) 141, 276, 308, 394
Mph1103I ATGCAT 1 cut(s) 327
MroXI GAANNNNTTC 1 cut(s) 325
MseI TTAA 3 cut(s) 36, 414, 459
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 125, 161
Mva1269I GAATGC 2 cut(s) 325, 327
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 19, 161
NciI CCSGG 1 cut(s) 161
NdeI CATATG 1 cut(s) 544
NdeII GATC 1 cut(s) 202
NlaIII CATG 3 cut(s) 209, 304, 309
NlaIV GGNNCC 2 cut(s) 129, 406
NsiI ATGCAT 1 cut(s) 327
NspV TTCGAA 1 cut(s) 137
PctI GAATGC 2 cut(s) 325, 327
PdmI GAANNNNTTC 1 cut(s) 325
PfeI GAWTC 2 cut(s) 46, 293
PkrI GCNGC 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 2 cut(s) 129, 406
PsrI GAACNNNNNNTAC 2 cut(s) 375, 407
PvuII CAGCTG 1 cut(s) 13
SaqAI TTAA 3 cut(s) 36, 414, 459
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 1 cut(s) 202
ScrFI CCNGG 2 cut(s) 125, 161
SduI GDGCHC 1 cut(s) 188
SetI ASST 6 cut(s) 15, 24, 126, 133, 217, 432
SfaNI GCATC 2 cut(s) 339, 346
SfuI TTCGAA 1 cut(s) 137
Sse9I AATT 4 cut(s) 79, 189, 453, 552
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 123, 159
TaqI TCGA 3 cut(s) 83, 137, 340
TasI AATT 4 cut(s) 79, 189, 453, 552
TfiI GAWTC 2 cut(s) 46, 293
Tru1I TTAA 3 cut(s) 36, 414, 459
Tru9I TTAA 3 cut(s) 36, 414, 459
TscAI CASTG 2 cut(s) 108, 175
TseI GCWGC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 20, 131, 453
TspRI CASTG 2 cut(s) 108, 175
XapI RAATTY 2 cut(s) 79, 453
XmnI GAANNNNTTC 1 cut(s) 325
XspI CTAG 1 cut(s) 431
Zsp2I ATGCAT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.