RchiOBHm_Chr7g0192061

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000761
Physical Location & Seq
Reverse (-)
10667264 .. 10668518
1255 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17163

Sequence Viewer

Length: 639 bp
ATGTCAATAGTGTTGCCTCTGCTATGCGCTTCCAAGTTGCCTCGTCAAAAACTTTGCCAAGTTTTGGTGACTAGAGGAGAAGATACTGCTCTTAGCCAGTTAAGCCCACAGGAAAAGAAAACAGTTGAAGCAATTGTGAAAGCAAGCGAGTATCCCTTATCAATTGTGGTAGTTGGAGTTGGAGATGGACCATGGGATATGATGGAGAAATTTGAGGGTAAAATTCCTCGACGGGCCTTTGATAACTTTCGGGCAAGTACTTGTAGTCTTTTTGTGAATGCTGCAGACATTTTCTCAAAGAATATGGACAGCTCAAGAAAAGACGTAAAATTTGCTTCTGCAGCCTTGCTGCAATTACCCTCTCAGTATAAAGCAACACAAGAGCTCAGCATATTGGGTGCTACAAGAGAGAATGCGGTGCGCAGGATTCCTCTTCCCCCTCCTCGTTACAGTTCAGGCTTTGTTGGCAGTGAAAGAGCACCACATGCTTCTGACAATCATCTCTGTCCCGTTTGCCTAACCAATGAGAAAAATATGGCATTTGGTTGCGGACATCAGACATGCTGTGACTGTGGACCAGATCTTGAGACGTGCCCGATTTGCAGAAGCGCCATCTACCTCAGGATAAAACTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

212

Amino Acids

23.31

Weight (kDa)

8.63

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Copine PF07002 22 - 132 8.5e-21 Copine
zf-C3HC4_3 PF13920 166 - 205 1.6e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 422
AccB7I CCANNNNNTGG 1 cut(s) 64
AciI CCGC 2 cut(s) 416, 549
AcsI RAATTY 3 cut(s) 209, 222, 329
AfaI GTAC 1 cut(s) 259
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 1 cut(s) 128
AjiI CACGTC 1 cut(s) 591
AluBI AGCT 2 cut(s) 312, 385
AluI AGCT 2 cut(s) 312, 385
Alw21I GWGCWC 2 cut(s) 387, 481
Alw26I GTCTC 1 cut(s) 581
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 3 cut(s) 281, 341, 349
ApoI RAATTY 3 cut(s) 209, 222, 329
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
AspLEI GCGC 3 cut(s) 29, 423, 611
AspS9I GGNCC 3 cut(s) 188, 234, 575
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 2 cut(s) 188, 575
AxyI CCTNAGG 1 cut(s) 620
BaeGI GKGCMC 1 cut(s) 596
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 2 cut(s) 387, 481
BbvI GCAGC 3 cut(s) 268, 336, 353
BccI CCATC 3 cut(s) 179, 196, 620
BciVI GTATCC 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 581
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 2 cut(s) 282, 339
BfoI RGCGCY 1 cut(s) 612
BfuI GTATCC 1 cut(s) 162
BglII AGATCT 1 cut(s) 580
BisI GCNGC 3 cut(s) 282, 342, 350
BlpI GCTNAGC 1 cut(s) 386
BlsI GCNGC 3 cut(s) 283, 343, 351
BmcAI AGTACT 1 cut(s) 259
Bme18I GGWCC 2 cut(s) 188, 575
BmgBI CACGTC 1 cut(s) 591
BmgT120I GGNCC 3 cut(s) 188, 234, 575
Bpu1102I GCTNAGC 1 cut(s) 386
BpuEI CTTGAG 2 cut(s) 298, 605
BsaJI CCNNGG 1 cut(s) 191
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 97
Bse21I CCTNAGG 1 cut(s) 620
BseDI CCNNGG 1 cut(s) 191
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMII CTCAG 3 cut(s) 377, 400, 634
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 90, 432
BseSI GKGCMC 1 cut(s) 596
BseXI GCAGC 3 cut(s) 268, 336, 353
BshFI GGCC 1 cut(s) 236
BsiHKAI GWGCWC 2 cut(s) 387, 481
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 581
BsmBI CGTCTC 1 cut(s) 581
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 2 cut(s) 283, 418
BsnI GGCC 1 cut(s) 236
Bsp1286I GDGCHC 3 cut(s) 387, 481, 596
Bsp143I GATC 1 cut(s) 580
Bsp1720I GCTNAGC 1 cut(s) 386
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 2 cut(s) 416, 549
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 3 cut(s) 376, 399, 633
BspMAI CTGCAG 2 cut(s) 286, 343
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 191
BssMI GATC 1 cut(s) 580
BssT1I CCWWGG 1 cut(s) 191
Bst4CI ACNGT 3 cut(s) 124, 452, 572
Bst6I CTCTTC 1 cut(s) 438
BstAPI GCANNNNNTGC 1 cut(s) 485
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 4 cut(s) 92, 363, 386, 620
BstDSI CCRYGG 1 cut(s) 191
BstH2I RGCGCY 1 cut(s) 612
BstHHI GCGC 3 cut(s) 29, 423, 611
BstKTI GATC 1 cut(s) 583
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 1 cut(s) 580
BstMWI GCNNNNNNNGC 5 cut(s) 102, 341, 465, 485, 600
BstNSI RCATGY 2 cut(s) 488, 564
BstSFI CTRYAG 2 cut(s) 282, 339
BstSLI GKGCMC 1 cut(s) 596
BstV1I GCAGC 3 cut(s) 268, 336, 353
BstX2I RGATCY 1 cut(s) 580
BstYI RGATCY 1 cut(s) 580
Bsu36I CCTNAGG 1 cut(s) 620
BsuI GTATCC 1 cut(s) 162
BsuRI GGCC 1 cut(s) 236
BtgI CCRYGG 1 cut(s) 191
BtrI CACGTC 1 cut(s) 591
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 145
CfoI GCGC 3 cut(s) 29, 423, 611
Cfr13I GGNCC 3 cut(s) 188, 234, 575
Csp6I GTAC 1 cut(s) 258
CviAII CATG 3 cut(s) 192, 485, 561
CviJI RGCY 7 cut(s) 96, 105, 236, 312, 344, 385, 459
CviKI_1 RGCY 7 cut(s) 96, 105, 236, 312, 344, 385, 459
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 4 cut(s) 92, 363, 386, 620
DpnI GATC 1 cut(s) 582
DpnII GATC 1 cut(s) 580
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 188, 575
Eco53kI GAGCTC 1 cut(s) 385
Eco81I CCTNAGG 1 cut(s) 620
EcoICRI GAGCTC 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 191
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 191
Esp3I CGTCTC 1 cut(s) 581
FaeI CATG 3 cut(s) 195, 488, 564
FaiI YATR 9 cut(s) 25, 193, 200, 305, 369, 392, 486, 536, 562
FaqI GGGAC 1 cut(s) 492
FatI CATG 3 cut(s) 191, 484, 560
Fnu4HI GCNGC 3 cut(s) 282, 342, 350
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 3 cut(s) 282, 342, 350
FspBI CTAG 1 cut(s) 72
FspI TGCGCA 1 cut(s) 422
GlaI GCGC 3 cut(s) 28, 422, 610
GluI GCNGC 3 cut(s) 282, 342, 350
HaeII RGCGCY 1 cut(s) 612
HaeIII GGCC 1 cut(s) 236
HhaI GCGC 3 cut(s) 29, 423, 611
Hin1II CATG 3 cut(s) 195, 488, 564
Hin6I GCGC 3 cut(s) 27, 421, 609
HinP1I GCGC 3 cut(s) 27, 421, 609
HinfI GANTC 1 cut(s) 427
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 1 cut(s) 575
Hpy188I TCNGA 2 cut(s) 493, 558
Hpy188III TCNNGA 3 cut(s) 315, 584, 622
Hpy8I GTNNAC 1 cut(s) 575
Hpy99I CGWCG 1 cut(s) 234
HpyCH4III ACNGT 3 cut(s) 124, 452, 572
HpyCH4IV ACGT 2 cut(s) 324, 590
HpyCH4V TGCA 4 cut(s) 284, 341, 352, 603
HpyF10VI GCNNNNNNNGC 5 cut(s) 102, 341, 465, 485, 600
HpyF3I CTNAG 4 cut(s) 92, 363, 386, 620
HpySE526I ACGT 2 cut(s) 324, 590
Hsp92II CATG 3 cut(s) 195, 488, 564
HspAI GCGC 3 cut(s) 27, 421, 609
Kzo9I GATC 1 cut(s) 580
LpnPI CCDG 6 cut(s) 95, 110, 409, 441, 591, 607
Lsp1109I GCAGC 3 cut(s) 268, 336, 353
MaeI CTAG 1 cut(s) 72
MaeII ACGT 2 cut(s) 324, 590
MaeIII GTNAC 3 cut(s) 67, 446, 566
MalI GATC 1 cut(s) 582
MboI GATC 1 cut(s) 580
MboII GAAGA 2 cut(s) 92, 425
MfeI CAATTG 2 cut(s) 132, 162
MflI RGATCY 1 cut(s) 580
MhlI GDGCHC 3 cut(s) 387, 481, 596
MluCI AATT 6 cut(s) 132, 162, 209, 222, 329, 353
MmeI TCCRAC 2 cut(s) 154, 160
MseI TTAA 1 cut(s) 101
MunI CAATTG 2 cut(s) 132, 162
Mva1269I GAATGC 2 cut(s) 283, 418
MwoI GCNNNNNNNGC 5 cut(s) 102, 341, 465, 485, 600
NcoI CCATGG 1 cut(s) 191
NdeII GATC 1 cut(s) 580
NlaIII CATG 3 cut(s) 195, 488, 564
NmuCI GTSAC 2 cut(s) 67, 566
NsbI TGCGCA 1 cut(s) 422
NspI RCATGY 2 cut(s) 488, 564
PctI GAATGC 2 cut(s) 283, 418
PfeI GAWTC 1 cut(s) 427
PflMI CCANNNNNTGG 1 cut(s) 64
PkrI GCNGC 3 cut(s) 283, 343, 351
Psp124BI GAGCTC 1 cut(s) 387
PspPI GGNCC 3 cut(s) 188, 234, 575
PstI CTGCAG 2 cut(s) 286, 343
PsuI RGATCY 1 cut(s) 580
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 3 cut(s) 282, 342, 350
Sau3AI GATC 1 cut(s) 580
Sau96I GGNCC 3 cut(s) 188, 234, 575
ScaI AGTACT 1 cut(s) 259
SduI GDGCHC 3 cut(s) 387, 481, 596
SetI ASST 5 cut(s) 314, 327, 387, 593, 621
SfcI CTRYAG 2 cut(s) 282, 339
SinI GGWCC 2 cut(s) 188, 575
SmlI CTYRAG 2 cut(s) 313, 584
SmoI CTYRAG 2 cut(s) 313, 584
Sse9I AATT 6 cut(s) 132, 162, 209, 222, 329, 353
SsiI CCGC 2 cut(s) 416, 549
SspMI CTAG 1 cut(s) 72
SstI GAGCTC 1 cut(s) 387
StyI CCWWGG 1 cut(s) 191
TaaI ACNGT 3 cut(s) 124, 452, 572
TaiI ACGT 2 cut(s) 327, 593
TaqI TCGA 1 cut(s) 229
TasI AATT 6 cut(s) 132, 162, 209, 222, 329, 353
TatI WGTACW 1 cut(s) 257
TfiI GAWTC 1 cut(s) 427
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 1 cut(s) 475
TseFI GTSAC 2 cut(s) 67, 566
TseI GCWGC 3 cut(s) 281, 341, 349
Tsp45I GTSAC 2 cut(s) 67, 566
TspRI CASTG 1 cut(s) 475
Van91I CCANNNNNTGG 1 cut(s) 64
VpaK11BI GGWCC 2 cut(s) 188, 575
XapI RAATTY 3 cut(s) 209, 222, 329
XceI RCATGY 2 cut(s) 488, 564
XspI CTAG 1 cut(s) 72
ZrmI AGTACT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.