Rmu_ssc0000421.1_g000038

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000421.1
OG0000761
Physical Location & Seq
Reverse (-)
154555 .. 157406
2852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000421.1_g000038.1.cds

Sequence Viewer

Length: 1434 bp
atgggttgtcaagcttcaacattcagattttctagaaggtctcgtcggcttgcgccacctccacccccgtcgctcccgcccctgcctccacctaaaagctatgagttggagaacaagtactcaaacacagatgctaatctgggcaggcagctggatgaggacgaggatgatgatgttaatgttgatgttgatgttgatgttggtgttggtgttggtgttggtgttgttgttgatgatgatgatgttgatattgttgttgatgttggtgttggtgttgttgttgatgatgatgatgatgttgatattgttgttgttgttgttgttgatattgatgttgttgatgatgatgatgctattgagtctgttattggtatagaatggaattctccagctgtttatgatgtttaccctgatcaagatgacaagcctgtgggtaatgaggtcacagatcaattgaagaatgccggtctggagtctgccaaacttattcttggtattgatttcacaaagagcaatgagctgagaggtgcaaggtcctgtaacgggaagagcttgcattacattggagcagagcaaaatccctatgaacaagcaatatcgatcactgggaaaacattgcagtcctttgataaggataggatgattccctgttttggatttggagatgcatcaaccaatgaccaggaagttttcagtttctatccagatgagagtgctcgctgcaatggatttgaagaagcaataaaacgatatagagaagtagtccaacagctacaacttggcgggcctaaatcatttgcccctatcattgaaatggccatgacactagttgaacagagtagtggccagtaccatgtgttggtgatactagctgatgggctggtgactggaggagatactgctcttacccagttaagcccacaggaaaagaaaacagttgaagcaattgtgaaagcaagcgagtatcccttatcaattgtggtagttggagttggagatggaccatgggatatgatgaagaaatttgagcgtaaaatccctcgacgggcctttgataactttcggtttgtgaatgctgcagacattttgtcaaagaatatggacagctcaaggaaagaagtaaaatttgcttctgcagccttgctgcaattagcctctcagtataaagcaacacaagagcttagcatattgggtgctacaacagagaatgcggtgcgtaggattcctcttccccctcctcgttacagttcaggctttgttggcagtgaacgagcacctcacgcttctgacaatcatctctgtcccgtttgcctatccaatgagaaaaatatggcatttcgttgcggacatcagacatgctgtgactgtggaccagatcttgcgaggtgcccgatttgcagaagcgccatcgacctcaggataaaactctattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

477

Amino Acids

52.18

Weight (kDa)

4.57

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1386
AccB7I CCANNNNNTGG 1 cut(s) 859
AciI CCGC 4 cut(s) 77, 783, 1211, 1344
AcoI YGGCCR 2 cut(s) 816, 844
AcsI RAATTY 3 cut(s) 382, 1022, 1124
AfaI GTAC 2 cut(s) 119, 851
AfiI CCNNNNNNNGG 4 cut(s) 543, 653, 859, 1045
AgsI TTSAA 6 cut(s) 18, 457, 734, 812, 833, 941
AhdI GACNNNNNGTC 1 cut(s) 1087
AhlI ACTAGT 1 cut(s) 826
AjnI CCWGG 1 cut(s) 681
Alw21I GWGCWC 2 cut(s) 718, 1276
Alw26I GTCTC 1 cut(s) 45
AoxI GGCC 4 cut(s) 785, 816, 844, 1047
ApeKI GCWGC 5 cut(s) 148, 720, 1076, 1136, 1144
ApoI RAATTY 3 cut(s) 382, 1022, 1124
AspLEI GCGC 2 cut(s) 55, 1406
AspS9I GGNCC 5 cut(s) 534, 785, 1001, 1047, 1370
AsuHPI GGTGA 2 cut(s) 874, 895
AvaII GGWCC 3 cut(s) 534, 1001, 1370
AxyI CCTNAGG 1 cut(s) 1415
BaeGI GKGCMC 1 cut(s) 1391
BalI TGGCCA 2 cut(s) 818, 846
BanI GGYRCC 1 cut(s) 1386
Bbv12I GWGCWC 2 cut(s) 718, 1276
BbvI GCAGC 5 cut(s) 160, 707, 1063, 1131, 1148
BccI CCATC 3 cut(s) 869, 992, 1415
BciT130I CCWGG 1 cut(s) 683
BciVI GTATCC 1 cut(s) 975
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 1 cut(s) 45
BcuI ACTAGT 1 cut(s) 826
BfaI CTAG 3 cut(s) 33, 827, 869
BfmI CTRYAG 2 cut(s) 1077, 1134
BfoI RGCGCY 1 cut(s) 1407
BfuI GTATCC 1 cut(s) 975
BglII AGATCT 1 cut(s) 1375
BisI GCNGC 5 cut(s) 149, 721, 1077, 1137, 1145
BlpI GCTNAGC 1 cut(s) 1181
BlsI GCNGC 5 cut(s) 150, 722, 1078, 1138, 1146
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 683
Bme18I GGWCC 3 cut(s) 534, 1001, 1370
BmeRI GACNNNNNGTC 1 cut(s) 1087
BmgT120I GGNCC 5 cut(s) 534, 785, 1001, 1047, 1370
BmiI GGNNCC 1 cut(s) 1388
BmrFI CCNGG 1 cut(s) 683
BmrI ACTGGG 2 cut(s) 615, 904
BmsI GCATC 4 cut(s) 121, 340, 655, 677
BmuI ACTGGG 2 cut(s) 615, 904
BpmI CTGGAG 3 cut(s) 372, 491, 909
Bpu1102I GCTNAGC 1 cut(s) 1181
BpuEI CTTGAG 1 cut(s) 1093
Bsa29I ATCGAT 1 cut(s) 599
BsaBI GATNNNNATC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 1004
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 4 cut(s) 543, 653, 859, 1045
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 4 cut(s) 610, 847, 892, 910
Bse21I CCTNAGG 1 cut(s) 1415
Bse3DI GCAATG 3 cut(s) 520, 614, 730
Bse8I GATNNNNATC 1 cut(s) 135
BseBI CCWGG 1 cut(s) 683
BseCI ATCGAT 1 cut(s) 599
BseDI CCNNGG 1 cut(s) 1004
BseGI GGATG 3 cut(s) 160, 172, 645
BseJI GATNNNNATC 1 cut(s) 135
BseLI CCNNNNNNNGG 4 cut(s) 543, 653, 859, 1045
BseMI GCAATG 3 cut(s) 520, 614, 730
BseMII CTCAG 3 cut(s) 512, 1172, 1429
BseNI ACTGG 4 cut(s) 610, 847, 892, 910
BseRI GAGGAG 2 cut(s) 906, 1227
BseSI GKGCMC 1 cut(s) 1391
BseXI GCAGC 5 cut(s) 160, 707, 1063, 1131, 1148
BshFI GGCC 4 cut(s) 787, 818, 846, 1049
BshNI GGYRCC 1 cut(s) 1386
BshVI ATCGAT 1 cut(s) 599
BsiHKAI GWGCWC 2 cut(s) 718, 1276
BsiSI CCGG 1 cut(s) 465
BslFI GGGAC 1 cut(s) 1287
BslI CCNNNNNNNGG 4 cut(s) 543, 653, 859, 1045
BsmAI GTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 1287
BsmI GAATGC 3 cut(s) 466, 1078, 1213
BsnI GGCC 4 cut(s) 787, 818, 846, 1049
Bso31I GGTCTC 1 cut(s) 45
Bsp1286I GDGCHC 3 cut(s) 718, 1276, 1391
Bsp143I GATC 4 cut(s) 412, 448, 600, 1375
Bsp1720I GCTNAGC 1 cut(s) 1181
Bsp19I CCATGG 1 cut(s) 1004
BspACI CCGC 4 cut(s) 77, 783, 1211, 1344
BspANI GGCC 4 cut(s) 787, 818, 846, 1049
BspCNI CTCAG 3 cut(s) 513, 1171, 1428
BspDI ATCGAT 1 cut(s) 599
BspLI GGNNCC 1 cut(s) 1388
BspMAI CTGCAG 2 cut(s) 1081, 1138
BspQI GCTCTTC 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 1386
BspTNI GGTCTC 1 cut(s) 45
BsrDI GCAATG 3 cut(s) 520, 614, 730
BsrFI RCCGGY 1 cut(s) 464
BsrI ACTGG 4 cut(s) 610, 847, 892, 910
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 1 cut(s) 1004
BssMI GATC 4 cut(s) 412, 448, 600, 1375
BssT1I CCWWGG 1 cut(s) 1004
Bst2UI CCWGG 1 cut(s) 683
Bst4CI ACNGT 3 cut(s) 937, 1247, 1367
Bst6I CTCTTC 2 cut(s) 542, 1233
BstC8I GCNNGC 6 cut(s) 51, 146, 554, 718, 785, 958
BstDEI CTNAG 4 cut(s) 521, 1158, 1181, 1415
BstDSI CCRYGG 1 cut(s) 1004
BstF5I GGATG 3 cut(s) 160, 172, 645
BstH2I RGCGCY 1 cut(s) 1407
BstHHI GCGC 2 cut(s) 55, 1406
BstKTI GATC 4 cut(s) 415, 451, 603, 1378
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 4 cut(s) 412, 448, 600, 1375
BstMWI GCNNNNNNNGC 4 cut(s) 1136, 1260, 1280, 1395
BstNI CCWGG 1 cut(s) 683
BstNSI RCATGY 1 cut(s) 1359
BstSCI CCNGG 1 cut(s) 681
BstSFI CTRYAG 2 cut(s) 1077, 1134
BstSLI GKGCMC 1 cut(s) 1391
BstV1I GCAGC 5 cut(s) 160, 707, 1063, 1131, 1148
BstX2I RGATCY 1 cut(s) 1375
BstYI RGATCY 1 cut(s) 1375
Bsu15I ATCGAT 1 cut(s) 599
Bsu36I CCTNAGG 1 cut(s) 1415
BsuI GTATCC 1 cut(s) 975
BsuRI GGCC 4 cut(s) 787, 818, 846, 1049
BsuTUI ATCGAT 1 cut(s) 599
BtgI CCRYGG 1 cut(s) 1004
BtsCI GGATG 3 cut(s) 160, 172, 645
BtsI GCAGTG 1 cut(s) 1270
BtsIMutI CAGTG 2 cut(s) 603, 1270
Cac8I GCNNGC 6 cut(s) 51, 146, 554, 718, 785, 958
CfoI GCGC 2 cut(s) 55, 1406
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 5 cut(s) 534, 785, 1001, 1047, 1370
ClaI ATCGAT 1 cut(s) 599
Csp6I GTAC 2 cut(s) 118, 850
CviAII CATG 4 cut(s) 820, 854, 1005, 1356
CviQI GTAC 2 cut(s) 118, 850
DdeI CTNAG 4 cut(s) 521, 1158, 1181, 1415
DpnI GATC 4 cut(s) 414, 450, 602, 1377
DpnII GATC 4 cut(s) 412, 448, 600, 1375
DriI GACNNNNNGTC 1 cut(s) 1087
EaeI YGGCCR 2 cut(s) 816, 844
Eam1104I CTCTTC 2 cut(s) 542, 1233
Eam1105I GACNNNNNGTC 1 cut(s) 1087
EarI CTCTTC 2 cut(s) 542, 1233
Eco130I CCWWGG 1 cut(s) 1004
Eco31I GGTCTC 1 cut(s) 45
Eco47I GGWCC 3 cut(s) 534, 1001, 1370
Eco81I CCTNAGG 1 cut(s) 1415
EcoO109I RGGNCCY 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 681
EcoT14I CCWWGG 1 cut(s) 1004
EcoT22I ATGCAT 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 1004
FaeI CATG 4 cut(s) 823, 857, 1008, 1359
FaqI GGGAC 1 cut(s) 1287
FatI CATG 4 cut(s) 819, 853, 1004, 1355
FauI CCCGC 2 cut(s) 84, 776
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 5 cut(s) 149, 721, 1077, 1137, 1145
FokI GGATG 3 cut(s) 167, 179, 652
Fsp4HI GCNGC 5 cut(s) 149, 721, 1077, 1137, 1145
FspBI CTAG 3 cut(s) 33, 827, 869
GlaI GCGC 2 cut(s) 54, 1405
GluI GCNGC 5 cut(s) 149, 721, 1077, 1137, 1145
GsuI CTGGAG 3 cut(s) 372, 491, 909
HaeII RGCGCY 1 cut(s) 1407
HaeIII GGCC 4 cut(s) 787, 818, 846, 1049
HapII CCGG 1 cut(s) 465
HhaI GCGC 2 cut(s) 55, 1406
Hin1II CATG 4 cut(s) 823, 857, 1008, 1359
Hin6I GCGC 2 cut(s) 53, 1404
HinP1I GCGC 2 cut(s) 53, 1404
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 4 cut(s) 359, 473, 643, 1222
HpaII CCGG 1 cut(s) 465
HphI GGTGA 2 cut(s) 874, 895
Hpy166II GTNNAC 3 cut(s) 406, 1268, 1370
Hpy188I TCNGA 3 cut(s) 26, 1288, 1353
Hpy188III TCNNGA 5 cut(s) 33, 416, 470, 704, 1417
Hpy8I GTNNAC 3 cut(s) 406, 1268, 1370
Hpy99I CGWCG 3 cut(s) 48, 73, 1047
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 3 cut(s) 937, 1247, 1367
HpyCH4V TGCA 9 cut(s) 530, 556, 619, 668, 723, 1079, 1136, 1147, 1398
HpyF10VI GCNNNNNNNGC 4 cut(s) 1136, 1260, 1280, 1395
HpyF3I CTNAG 4 cut(s) 521, 1158, 1181, 1415
Hsp92II CATG 4 cut(s) 823, 857, 1008, 1359
HspAI GCGC 2 cut(s) 53, 1404
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 4 cut(s) 412, 448, 600, 1375
LguI GCTCTTC 1 cut(s) 542
LmnI GCTCC 2 cut(s) 78, 566
Lsp1109I GCAGC 5 cut(s) 160, 707, 1063, 1131, 1148
LweI GCATC 4 cut(s) 121, 340, 655, 677
MaeI CTAG 3 cut(s) 33, 827, 869
MaeIII GTNAC 5 cut(s) 442, 539, 883, 1241, 1361
MalI GATC 4 cut(s) 414, 450, 602, 1377
MboI GATC 4 cut(s) 412, 448, 600, 1375
MboII GAAGA 5 cut(s) 469, 559, 746, 1030, 1220
MfeI CAATTG 3 cut(s) 452, 945, 975
MflI RGATCY 1 cut(s) 1375
MhlI GDGCHC 3 cut(s) 718, 1276, 1391
MlsI TGGCCA 2 cut(s) 818, 846
MluCI AATT 7 cut(s) 382, 452, 945, 975, 1022, 1124, 1148
MluNI TGGCCA 2 cut(s) 818, 846
MlyI GAGTC 2 cut(s) 368, 482
MmeI TCCRAC 4 cut(s) 87, 790, 967, 973
Mox20I TGGCCA 2 cut(s) 818, 846
Mph1103I ATGCAT 1 cut(s) 670
MscI TGGCCA 2 cut(s) 818, 846
MseI TTAA 2 cut(s) 177, 914
MslI CAYNNNNRTG 1 cut(s) 812
Msp20I TGGCCA 2 cut(s) 818, 846
MspA1I CMGCKG 2 cut(s) 151, 392
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 1 cut(s) 683
MunI CAATTG 3 cut(s) 452, 945, 975
Mva1269I GAATGC 3 cut(s) 466, 1078, 1213
MvaI CCWGG 1 cut(s) 683
MwoI GCNNNNNNNGC 4 cut(s) 1136, 1260, 1280, 1395
NcoI CCATGG 1 cut(s) 1004
NdeII GATC 4 cut(s) 412, 448, 600, 1375
NlaIII CATG 4 cut(s) 823, 857, 1008, 1359
NlaIV GGNNCC 1 cut(s) 1388
NmuCI GTSAC 3 cut(s) 442, 883, 1361
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 1 cut(s) 1359
PciSI GCTCTTC 1 cut(s) 542
PctI GAATGC 3 cut(s) 466, 1078, 1213
PfeI GAWTC 2 cut(s) 643, 1222
PflMI CCANNNNNTGG 1 cut(s) 859
PkrI GCNGC 5 cut(s) 150, 722, 1078, 1138, 1146
PleI GAGTC 2 cut(s) 367, 481
PpsI GAGTC 2 cut(s) 367, 481
PpuMI RGGWCCY 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 681
PspGI CCWGG 1 cut(s) 681
PspN4I GGNNCC 1 cut(s) 1388
PspPI GGNCC 5 cut(s) 534, 785, 1001, 1047, 1370
PspPPI RGGWCCY 1 cut(s) 534
PstI CTGCAG 2 cut(s) 1081, 1138
PsuI RGATCY 1 cut(s) 1375
PvuII CAGCTG 2 cut(s) 151, 392
RsaI GTAC 2 cut(s) 119, 851
RsaNI GTAC 2 cut(s) 118, 850
RseI CAYNNNNRTG 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 542
SaqAI TTAA 2 cut(s) 177, 914
SatI GCNGC 5 cut(s) 149, 721, 1077, 1137, 1145
Sau3AI GATC 4 cut(s) 412, 448, 600, 1375
Sau96I GGNCC 5 cut(s) 534, 785, 1001, 1047, 1370
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 2 cut(s) 368, 482
ScrFI CCNGG 1 cut(s) 683
SduI GDGCHC 3 cut(s) 718, 1276, 1391
SfaNI GCATC 4 cut(s) 121, 340, 655, 677
SfcI CTRYAG 2 cut(s) 1077, 1134
SinI GGWCC 3 cut(s) 534, 1001, 1370
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 1 cut(s) 1108
SmoI CTYRAG 1 cut(s) 1108
SpeI ACTAGT 1 cut(s) 826
Sse9I AATT 7 cut(s) 382, 452, 945, 975, 1022, 1124, 1148
SsiI CCGC 4 cut(s) 77, 783, 1211, 1344
SspMI CTAG 3 cut(s) 33, 827, 869
StyD4I CCNGG 1 cut(s) 681
StyI CCWWGG 1 cut(s) 1004
TaaI ACNGT 3 cut(s) 937, 1247, 1367
TaqI TCGA 3 cut(s) 599, 1042, 1410
TasI AATT 7 cut(s) 382, 452, 945, 975, 1022, 1124, 1148
TatI WGTACW 1 cut(s) 117
TfiI GAWTC 2 cut(s) 643, 1222
Tru1I TTAA 2 cut(s) 177, 914
Tru9I TTAA 2 cut(s) 177, 914
TscAI CASTG 2 cut(s) 610, 1270
TseFI GTSAC 3 cut(s) 442, 883, 1361
TseI GCWGC 5 cut(s) 148, 720, 1076, 1136, 1144
Tsp45I GTSAC 3 cut(s) 442, 883, 1361
TspDTI ATGAA 2 cut(s) 600, 1031
TspRI CASTG 2 cut(s) 610, 1270
Van91I CCANNNNNTGG 1 cut(s) 859
VpaK11BI GGWCC 3 cut(s) 534, 1001, 1370
XapI RAATTY 3 cut(s) 382, 1022, 1124
XbaI TCTAGA 1 cut(s) 32
XceI RCATGY 1 cut(s) 1359
XspI CTAG 3 cut(s) 33, 827, 869
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.