Rorug06G0513000

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000761
Physical Location & Seq
Reverse (-)
62776183 .. 62779615
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0513000.1

Sequence Viewer

Length: 789 bp
ATGTTACAAGACAACCTGAATGAATTGATTGAGAGAAAGGTCACTGTTATGAATGAATTGCAAAGCTTAAGAGAGAAAATTGATAATGAAGGTGCTAAAGCCGTGGAGGACAAGTTGGTATCGCTTAAGAAGTCATTGAAGGAGCTGGAGAGGGAAGAAAATGAAGTTGAGTCTGTAAGTAATTCTGAATTGGATGCTGAAGTTCGTGAATTGGAGGAGCAAATAGAAAATGGGTATGATAGTCAATCACTCTCTGGTAAATTGAATCGGATGTTGAGTGAATCAGAGGAGAAAATTGATTTAGCTAAAAAGGAACTTGCAGCTAGATTAAGGGCAGTGTTGGCAGTACAGCGCAAGATTGATGATGTTCCATGCCAATCAGAATTTGTCCAGTATGATCACCGGCTTTCAGAATTGAATGCTCAAATTCAAGGAAAACTTCGACAGACTCGTAAATACTATGCCACATACAATGCACTTTTGGAGATAAAAGAAATGATGCTAAAGGAAGCATCTTTGCTGAATTCAATAAGTTCGCAGTTTCAAGATGCATTCACTAGCATGGATGGTCGCATGAAACTCATCAATTCGATGGAGGGAATTATGAAGGGCAGTCAACAGAAGCTGAAAAAGGTAGAACAAGGGCTTCAAGAAGAGCAAAAAGTTTGTGATGCTCTCAAAGCAAGGCATGTTGCAGCAACTGCAGAGCAAAGACGCTGTTATTCCCTCTTAAAAGCTTTTCAGGAGGAATGTGCAAAGAACGAGCTACTCCAAAAAAGTTCCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

262

Amino Acids

30.04

Weight (kDa)

5.36

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC93_CC PF09762 5 - 259 1.3e-44 CCDC93 coiled-coil domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 383, 426, 523
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 1 cut(s) 348
AflII CTTAAG 2 cut(s) 67, 125
AgsI TTSAA 7 cut(s) 139, 265, 418, 431, 528, 545, 650
AluBI AGCT 7 cut(s) 66, 145, 305, 323, 625, 737, 766
AluI AGCT 7 cut(s) 66, 145, 305, 323, 625, 737, 766
AlwNI CAGNNNCTG 2 cut(s) 625, 701
ApeKI GCWGC 2 cut(s) 320, 695
ApoI RAATTY 3 cut(s) 383, 426, 523
AspLEI GCGC 1 cut(s) 354
AsuHPI GGTGA 1 cut(s) 392
BbvI GCAGC 2 cut(s) 332, 707
BccI CCATC 2 cut(s) 560, 586
BceAI ACGGC 1 cut(s) 86
BclI TGATCA 1 cut(s) 397
BfaI CTAG 2 cut(s) 324, 558
BfmI CTRYAG 1 cut(s) 702
BfrI CTTAAG 2 cut(s) 67, 125
BisI GCNGC 2 cut(s) 321, 696
BlsI GCNGC 2 cut(s) 322, 697
BmsI GCATC 5 cut(s) 184, 489, 521, 538, 661
BpmI CTGGAG 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bse118I RCCGGY 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 391
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 3 cut(s) 199, 276, 571
BseNI ACTGG 1 cut(s) 391
BseRI GAGGAG 2 cut(s) 230, 302
BseXI GCAGC 2 cut(s) 332, 707
BsiSI CCGG 1 cut(s) 403
BsmI GAATGC 2 cut(s) 424, 551
Bsp143I GATC 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 706
BspQI GCTCTTC 1 cut(s) 648
BspTI CTTAAG 2 cut(s) 67, 125
BsrFI RCCGGY 1 cut(s) 402
BsrI ACTGG 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 402
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 1 cut(s) 397
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 1 cut(s) 648
BstAFI CTTAAG 2 cut(s) 67, 125
BstAPI GCANNNNNTGC 1 cut(s) 701
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 3 cut(s) 199, 276, 571
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 1 cut(s) 400
BstMBI GATC 1 cut(s) 397
BstMWI GCNNNNNNNGC 3 cut(s) 341, 680, 701
BstNSI RCATGY 1 cut(s) 692
BstSFI CTRYAG 1 cut(s) 702
BstV1I GCAGC 2 cut(s) 332, 707
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 199, 276, 571
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 42, 342
CaiI CAGNNNCTG 2 cut(s) 625, 701
CfoI GCGC 1 cut(s) 354
Cfr10I RCCGGY 1 cut(s) 402
CseI GACGC 1 cut(s) 723
Csp6I GTAC 1 cut(s) 347
CviAII CATG 4 cut(s) 372, 562, 574, 689
CviQI GTAC 1 cut(s) 347
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 219
EcoRI GAATTC 1 cut(s) 523
EcoT22I ATGCAT 1 cut(s) 553
FaeI CATG 4 cut(s) 375, 565, 577, 692
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FatI CATG 4 cut(s) 371, 561, 573, 688
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 321, 696
FokI GGATG 3 cut(s) 206, 283, 578
Fsp4HI GCNGC 2 cut(s) 321, 696
FspBI CTAG 2 cut(s) 324, 558
GlaI GCGC 1 cut(s) 353
GluI GCNGC 2 cut(s) 321, 696
GsuI CTGGAG 1 cut(s) 167
HapII CCGG 1 cut(s) 403
HgaI GACGC 1 cut(s) 723
HhaI GCGC 1 cut(s) 354
Hin1II CATG 4 cut(s) 375, 565, 577, 692
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HincII GTYRAC 1 cut(s) 617
HindII GTYRAC 1 cut(s) 617
HindIII AAGCTT 2 cut(s) 64, 735
HinfI GANTC 4 cut(s) 170, 265, 281, 448
HpaII CCGG 1 cut(s) 403
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 1 cut(s) 617
Hpy188I TCNGA 5 cut(s) 187, 270, 286, 382, 412
Hpy188III TCNNGA 4 cut(s) 206, 545, 650, 743
Hpy8I GTNNAC 1 cut(s) 617
HpyAV CCTTC 3 cut(s) 83, 133, 601
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4V TGCA 7 cut(s) 61, 320, 476, 551, 695, 704, 755
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 680, 701
Hsp92II CATG 4 cut(s) 375, 565, 577, 692
HspAI GCGC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 1 cut(s) 397
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 2 cut(s) 142, 217
LpnPI CCDG 6 cut(s) 29, 131, 240, 404, 416, 728
Lsp1109I GCAGC 2 cut(s) 332, 707
LweI GCATC 5 cut(s) 184, 489, 521, 538, 661
MaeI CTAG 2 cut(s) 324, 558
MaeIII GTNAC 2 cut(s) 3, 40
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 2 cut(s) 167, 665
MlyI GAGTC 2 cut(s) 179, 442
MnlI CCTC 7 cut(s) 100, 144, 208, 280, 589, 737, 739
Mph1103I ATGCAT 1 cut(s) 553
MseI TTAA 4 cut(s) 68, 126, 329, 731
MslI CAYNNNNRTG 2 cut(s) 47, 560
MspCI CTTAAG 2 cut(s) 67, 125
MspI CCGG 1 cut(s) 403
Mva1269I GAATGC 2 cut(s) 424, 551
MwoI GCNNNNNNNGC 3 cut(s) 341, 680, 701
NdeII GATC 1 cut(s) 397
NlaIII CATG 4 cut(s) 375, 565, 577, 692
NmuCI GTSAC 1 cut(s) 40
NsiI ATGCAT 1 cut(s) 553
NspI RCATGY 1 cut(s) 692
PciSI GCTCTTC 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 448
PctI GAATGC 2 cut(s) 424, 551
PfeI GAWTC 2 cut(s) 265, 281
PkrI GCNGC 2 cut(s) 322, 697
PleI GAGTC 2 cut(s) 178, 442
PpsI GAGTC 2 cut(s) 178, 442
PstI CTGCAG 1 cut(s) 706
PstNI CAGNNNCTG 2 cut(s) 625, 701
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
RseI CAYNNNNRTG 2 cut(s) 47, 560
SapI GCTCTTC 1 cut(s) 648
SaqAI TTAA 4 cut(s) 68, 126, 329, 731
SatI GCNGC 2 cut(s) 321, 696
Sau3AI GATC 1 cut(s) 397
SchI GAGTC 2 cut(s) 179, 442
SfaNI GCATC 5 cut(s) 184, 489, 521, 538, 661
SfcI CTRYAG 1 cut(s) 702
SmiMI CAYNNNNRTG 2 cut(s) 47, 560
SmlI CTYRAG 2 cut(s) 67, 125
SmoI CTYRAG 2 cut(s) 67, 125
SspMI CTAG 2 cut(s) 324, 558
TaaI ACNGT 1 cut(s) 46
TaqI TCGA 2 cut(s) 442, 590
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 2 cut(s) 265, 281
Tru1I TTAA 4 cut(s) 68, 126, 329, 731
Tru9I TTAA 4 cut(s) 68, 126, 329, 731
TscAI CASTG 2 cut(s) 49, 342
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 2 cut(s) 320, 695
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 7 cut(s) 36, 65, 69, 102, 177, 590, 620
TspRI CASTG 2 cut(s) 49, 342
Vha464I CTTAAG 2 cut(s) 67, 125
XapI RAATTY 3 cut(s) 383, 426, 523
XceI RCATGY 1 cut(s) 692
XspI CTAG 2 cut(s) 324, 558
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.