FvH4_5g06630

Domain of unknown function (DUF1929)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000761
Physical Location & Seq
Forward (+)
3913289 .. 3915025
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06630.t1

Sequence Viewer

Length: 1737 bp
ATGGCGCGCTACAATCACTTCCTGATTGTATTAAATTCCATGATTGCTGCTTTGTTCTTCACATTGCATTGTATTGGATGTACTAATGCTAAGCATGCAGTTGAGGAGCCCTCGATTGCAGATGGGAAAGGGCAATGGCAACTGCTTCTGGACAACACTGAAGTGGTGGCAATGCATATGGCCCTAACTCACCGCAACACAGTCTTAATCTTTGATCAAACAGAAGCAGGCCCTTCCGGGCACCAGCTTCGAAGGCGCTACCATGGCACTAGGTGTACTCAAATGCGCAAGGACGTAGAAGACTCAGCATGCTATGCGCACTCGGTGGAGTACGACATTGCTAGTAACAAGCTGAGGCCTCTGACGCTTACGACTGATACTTGGTGCTCCTCGGGTTCTTTCTTGAGCAATGGGACACTTCTGCAAACCGGTGGACATGGAAGAGGAGGTCAGAGAGTTCGATACTTTAGTCCTTGTGGGGGAAATGATCACTGCGATTGGAGGGAATCGAAGCAGTCACTGACTAATGATCGTTGGTTTTCATCCAGTCTTAGACTCCCGGAGGAGGATTGGGTTATTGTTGTGGGTGGAAGAAAGGTATTTACTTATGAATTTATACCAAAGCAGTATTCCAATTACGAAGGCTCTTTTGACTTCCCATTCTTGCGCAACACATATCAAGAAGGAGAAGGAGGGAACAATCTATATCCATTCCTTCACATATGTCCGAATGGCAGATTGTTCATATTTGCCAACCGTGACTCGATTCTCTTTAACTACCGAGCAAACGTAGTGGTCAAGTACTTCCCACGCATACCCGGAGAAGGCTCTAGGAACTTCCCCAGCTCCGGCTCATCGGTCATTCTCCCTTTAGACTACACAAACGGCTTTGAAAAGGCTGAAGTCATGGTGTGCGGTGGTGCGGCCAATGGAGCTTATAGAGCCGCAAGAGAGGGGAGGTACTTGAAAGCCTTGAGCTCTTGTGGAAGAATGATGATCACAGGTAACAAACACAAGTGGAACATGGAGTACATGCCCGGTCCACGCCTCTTAAGCGACATGCTGCTACTCCCAACCGGAGATGTCCTGATCATCAATGGAGCTAAAAATGGTGCTGCAGGTTGGAACAATGCAAGAAATGCTTCACTCGAGCCTTACCTTTACCACCCAAGGAAGGCATTGAGCAGAAGGTTCTCAGTGCTCAAGGCCACAAGAATTCCTAGAATGTATCACTCCTCGGCGGCTCTTTTACCCGATGGGAGGGTTTTAGTCGCCGGCAGTAACCCTCACGATAGGTACATTTTCGAAAAGGTGGCATACCCAACGGAGCTTAGCCTACAAGCATTTGTTCCACCCTATATGGTTGGTCAGCACCATCAATCTAGACCTACTAATGTTACCGTACACTATGGTAAGTATGATTCTAATGGTGTGAGGTATGGGGGGGAGTTCAATGTGAGATTTTGGTTGGGGACTAATTCGACCAAATCGGTGGAGTTCACTGCTTATGCTCCGCCGTTCACGTCACACTCGATATCGATGAATCAGAGACTACTGAGGCTGAGGTGCAAGAGTTTGGTGAGGGATCAAGATGGATGGGTAGCTGCAGTGTTGGAGGCTCCTCCGTCTCCTTCTGTTGCGCCAGCTGGTTATTACATGCTCACTGTTGTTAATGGTGGAATTCCTAGCGTGTCTCAGTGGATGAGGATCCTAAATACTTCCGGAAATGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1929 Glyoxal_oxid_N

Protein Analysis

579

Amino Acids

64.69

Weight (kDa)

9.29

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxal_oxid_N PF07250 58 - 453 1.5e-166 Glyoxal oxidase N-terminus
GO-like_E_set PF09118 476 - 570 1.4e-22 Galactose oxidase-like, Early set domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 287, 318, 668
Acc36I ACCTGC 1 cut(s) 1109
AccB1I GGYRCC 1 cut(s) 240
AccII CGCG 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 1721
AciI CCGC 6 cut(s) 193, 915, 923, 945, 1241, 1514
AclWI GGATC 3 cut(s) 1593, 1702, 1715
AcoI YGGCCR 1 cut(s) 924
AcsI RAATTY 4 cut(s) 34, 611, 1215, 1680
AcuI CTGAAG 2 cut(s) 180, 921
AdeI CACNNNGTG 2 cut(s) 273, 325
AfaI GTAC 8 cut(s) 82, 277, 332, 803, 962, 1031, 1298, 1404
AfiI CCNNNNNNNGG 6 cut(s) 479, 565, 824, 848, 1174, 1260
AflII CTTAAG 1 cut(s) 1051
AgeI ACCGGT 1 cut(s) 428
AgsI TTSAA 3 cut(s) 893, 967, 1453
AjiI CACGTC 1 cut(s) 1524
AjuI GAANNNNNNNTTGG 2 cut(s) 613, 645
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 9 cut(s) 247, 352, 846, 935, 978, 1103, 1330, 1604, 1646
AluI AGCT 9 cut(s) 247, 352, 846, 935, 978, 1103, 1330, 1604, 1646
Alw21I GWGCWC 3 cut(s) 389, 980, 1203
Alw26I GTCTC 3 cut(s) 1543, 1632, 1698
AlwI GGATC 3 cut(s) 1593, 1702, 1715
AlwNI CAGNNNCTG 1 cut(s) 520
Ama87I CYCGRG 2 cut(s) 391, 1148
Aor13HI TCCGGA 1 cut(s) 1721
AoxI GGCC 5 cut(s) 180, 229, 356, 924, 1206
ApeKI GCWGC 4 cut(s) 47, 1063, 1115, 1604
ApoI RAATTY 4 cut(s) 34, 611, 1215, 1680
AsiGI ACCGGT 1 cut(s) 428
Asp700I GAANNNNTTC 2 cut(s) 1141, 1729
AspLEI GCGC 7 cut(s) 7, 9, 258, 288, 319, 669, 1642
AspS9I GGNCC 3 cut(s) 181, 230, 1040
AsuC2I CCSGG 4 cut(s) 238, 560, 819, 1038
AsuHPI GGTGA 2 cut(s) 182, 1591
AsuII TTCGAA 2 cut(s) 250, 1305
AvaI CYCGRG 2 cut(s) 391, 1148
AvaII GGWCC 1 cut(s) 1040
BaeGI GKGCMC 1 cut(s) 243
BaeI ACNNNNGTAYC 2 cut(s) 454, 487
BamHI GGATCC 1 cut(s) 1707
BanI GGYRCC 1 cut(s) 240
BanII GRGCYC 2 cut(s) 111, 980
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 3 cut(s) 389, 980, 1203
BbvCI CCTCAGC 2 cut(s) 353, 1562
BbvI GCAGC 4 cut(s) 34, 1050, 1102, 1591
BccI CCATC 5 cut(s) 116, 1250, 1383, 1586, 1590
BceAI ACGGC 2 cut(s) 901, 1501
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BclI TGATCA 4 cut(s) 214, 487, 996, 1089
BcnI CCSGG 4 cut(s) 238, 560, 819, 1038
BcoDI GTCTC 3 cut(s) 1543, 1632, 1698
BfaI CTAG 6 cut(s) 270, 342, 831, 1221, 1383, 1686
BfmI CTRYAG 2 cut(s) 1116, 1605
BfoI RGCGCY 1 cut(s) 259
BfrI CTTAAG 1 cut(s) 1051
BfuAI ACCTGC 1 cut(s) 1109
BisI GCNGC 7 cut(s) 48, 924, 945, 1064, 1116, 1242, 1605
BlpI GCTNAGC 2 cut(s) 90, 1331
BlsI GCNGC 7 cut(s) 49, 925, 946, 1065, 1117, 1243, 1606
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 4 cut(s) 238, 560, 819, 1038
Bme18I GGWCC 1 cut(s) 1040
BmeT110I CYCGRG 2 cut(s) 391, 1148
BmgBI CACGTC 1 cut(s) 1524
BmgT120I GGNCC 3 cut(s) 181, 230, 1040
BmiI GGNNCC 4 cut(s) 108, 242, 1620, 1709
BmrFI CCNGG 4 cut(s) 238, 560, 819, 1038
BpiI GAAGAC 1 cut(s) 306
BplI GAGNNNNNCTC 2 cut(s) 95, 127
Bpu10I CCTNAGC 2 cut(s) 353, 1562
Bpu1102I GCTNAGC 2 cut(s) 90, 1331
Bpu14I TTCGAA 2 cut(s) 250, 1305
BpuEI CTTGAG 3 cut(s) 424, 994, 1187
BpuMI CCSGG 4 cut(s) 238, 560, 819, 1038
Bsa29I ATCGAT 1 cut(s) 1538
BsaBI GATNNNNATC 1 cut(s) 1706
BsaJI CCNNGG 4 cut(s) 262, 390, 1169, 1236
BsaWI WCCGGW 3 cut(s) 428, 1076, 1721
Bsc4I CCNNNNNNNGG 6 cut(s) 479, 565, 824, 848, 1174, 1260
Bse118I RCCGGY 2 cut(s) 428, 1274
Bse1I ACTGG 1 cut(s) 546
Bse3DI GCAATG 5 cut(s) 62, 140, 177, 336, 415
Bse8I GATNNNNATC 1 cut(s) 1706
BseAI TCCGGA 1 cut(s) 1721
BseCI ATCGAT 1 cut(s) 1538
BseDI CCNNGG 4 cut(s) 262, 390, 1169, 1236
BseGI GGATG 4 cut(s) 83, 542, 1601, 1707
BseJI GATNNNNATC 1 cut(s) 1706
BseLI CCNNNNNNNGG 6 cut(s) 479, 565, 824, 848, 1174, 1260
BseMI GCAATG 5 cut(s) 62, 140, 177, 336, 415
BseMII CTCAG 6 cut(s) 318, 344, 1209, 1547, 1553, 1709
BseNI ACTGG 1 cut(s) 546
BsePI GCGCGC 1 cut(s) 5
BseRI GAGGAG 6 cut(s) 119, 379, 459, 578, 1225, 1611
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 4 cut(s) 34, 1050, 1102, 1591
BseYI CCCAGC 1 cut(s) 842
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 5 cut(s) 182, 231, 358, 926, 1208
BshNI GGYRCC 1 cut(s) 240
BshTI ACCGGT 1 cut(s) 428
BshVI ATCGAT 1 cut(s) 1538
BsiHKAI GWGCWC 3 cut(s) 389, 980, 1203
BsiHKCI CYCGRG 2 cut(s) 391, 1148
BsiSI CCGG 9 cut(s) 237, 429, 560, 819, 849, 1038, 1077, 1275, 1722
BslFI GGGAC 2 cut(s) 427, 1486
BslI CCNNNNNNNGG 6 cut(s) 479, 565, 824, 848, 1174, 1260
BsmAI GTCTC 3 cut(s) 1543, 1632, 1698
BsmBI CGTCTC 1 cut(s) 1632
BsmFI GGGAC 2 cut(s) 427, 1486
BsnI GGCC 5 cut(s) 182, 231, 358, 926, 1208
BsoBI CYCGRG 2 cut(s) 391, 1148
Bsp119I TTCGAA 2 cut(s) 250, 1305
Bsp1286I GDGCHC 5 cut(s) 111, 243, 389, 980, 1203
Bsp13I TCCGGA 1 cut(s) 1721
Bsp143I GATC 7 cut(s) 214, 487, 529, 996, 1089, 1585, 1707
Bsp1720I GCTNAGC 2 cut(s) 90, 1331
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 6 cut(s) 193, 915, 923, 945, 1241, 1514
BspANI GGCC 5 cut(s) 182, 231, 358, 926, 1208
BspCNI CTCAG 6 cut(s) 317, 345, 1208, 1548, 1554, 1708
BspDI ATCGAT 1 cut(s) 1538
BspEI TCCGGA 1 cut(s) 1721
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 4 cut(s) 108, 242, 1620, 1709
BspMAI CTGCAG 2 cut(s) 1120, 1609
BspMI ACCTGC 1 cut(s) 1109
BspPI GGATC 3 cut(s) 1593, 1702, 1715
BspT104I TTCGAA 2 cut(s) 250, 1305
BspT107I GGYRCC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 1051
BsrDI GCAATG 5 cut(s) 62, 140, 177, 336, 415
BsrFI RCCGGY 2 cut(s) 428, 1274
BsrI ACTGG 1 cut(s) 546
BssAI RCCGGY 2 cut(s) 428, 1274
BssECI CCNNGG 4 cut(s) 262, 390, 1169, 1236
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 7 cut(s) 214, 487, 529, 996, 1089, 1585, 1707
BssT1I CCWWGG 2 cut(s) 262, 1169
Bst4CI ACNGT 4 cut(s) 202, 758, 1402, 1666
Bst6I CTCTTC 1 cut(s) 436
BstAFI CTTAAG 1 cut(s) 1051
BstAPI GCANNNNNTGC 2 cut(s) 314, 1139
BstBI TTCGAA 2 cut(s) 250, 1305
BstC8I GCNNGC 6 cut(s) 7, 96, 229, 310, 1276, 1644
BstDEI CTNAG 9 cut(s) 90, 304, 353, 551, 1195, 1331, 1556, 1562, 1695
BstDSI CCRYGG 1 cut(s) 262
BstF5I GGATG 4 cut(s) 83, 542, 1601, 1707
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 7 cut(s) 7, 9, 258, 288, 319, 669, 1642
BstKTI GATC 7 cut(s) 217, 490, 532, 999, 1092, 1588, 1710
BstMAI GTCTC 3 cut(s) 1543, 1632, 1698
BstMBI GATC 7 cut(s) 214, 487, 529, 996, 1089, 1585, 1707
BstMWI GCNNNNNNNGC 9 cut(s) 95, 253, 264, 314, 364, 932, 941, 1053, 1139
BstNSI RCATGY 5 cut(s) 98, 312, 1036, 1063, 1660
BstSCI CCNGG 4 cut(s) 236, 558, 817, 1036
BstSFI CTRYAG 2 cut(s) 1116, 1605
BstSLI GKGCMC 1 cut(s) 243
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 4 cut(s) 34, 1050, 1102, 1591
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 1707
BstXI CCANNNNNNTGG 1 cut(s) 1492
BstYI RGATCY 1 cut(s) 1707
Bsu15I ATCGAT 1 cut(s) 1538
BsuRI GGCC 5 cut(s) 182, 231, 358, 926, 1208
BsuTUI ATCGAT 1 cut(s) 1538
BtgI CCRYGG 1 cut(s) 262
BtrI CACGTC 1 cut(s) 1524
BtsCI GGATG 4 cut(s) 83, 542, 1601, 1707
BtsI GCAGTG 3 cut(s) 490, 1500, 1614
BtsIMutI CAGTG 8 cut(s) 156, 490, 518, 1203, 1500, 1614, 1662, 1703
BveI ACCTGC 1 cut(s) 1109
Cac8I GCNNGC 6 cut(s) 7, 96, 229, 310, 1276, 1644
CaiI CAGNNNCTG 1 cut(s) 520
CfoI GCGC 7 cut(s) 7, 9, 258, 288, 319, 669, 1642
Cfr10I RCCGGY 2 cut(s) 428, 1274
Cfr13I GGNCC 3 cut(s) 181, 230, 1040
ClaI ATCGAT 1 cut(s) 1538
CseI GACGC 1 cut(s) 373
Csp6I GTAC 8 cut(s) 81, 276, 331, 802, 961, 1030, 1297, 1403
CspAI ACCGGT 1 cut(s) 428
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviQI GTAC 8 cut(s) 81, 276, 331, 802, 961, 1030, 1297, 1403
DdeI CTNAG 9 cut(s) 90, 304, 353, 551, 1195, 1331, 1556, 1562, 1695
DpnI GATC 7 cut(s) 216, 489, 531, 998, 1091, 1587, 1709
DpnII GATC 7 cut(s) 214, 487, 529, 996, 1089, 1585, 1707
DraIII CACNNNGTG 2 cut(s) 273, 325
EaeI YGGCCR 1 cut(s) 924
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
EciI GGCGGA 1 cut(s) 1503
Ecl136II GAGCTC 1 cut(s) 978
Eco130I CCWWGG 2 cut(s) 262, 1169
Eco147I AGGCCT 1 cut(s) 358
Eco24I GRGCYC 2 cut(s) 111, 980
Eco32I GATATC 1 cut(s) 1536
Eco47I GGWCC 1 cut(s) 1040
Eco53kI GAGCTC 1 cut(s) 978
Eco57I CTGAAG 2 cut(s) 180, 921
Eco88I CYCGRG 2 cut(s) 391, 1148
EcoICRI GAGCTC 1 cut(s) 978
EcoO109I RGGNCCY 1 cut(s) 230
EcoRI GAATTC 2 cut(s) 1215, 1680
EcoRV GATATC 1 cut(s) 1536
EcoT14I CCWWGG 2 cut(s) 262, 1169
EcoT22I ATGCAT 1 cut(s) 177
EcoT38I GRGCYC 2 cut(s) 111, 980
ErhI CCWWGG 2 cut(s) 262, 1169
Esp3I CGTCTC 1 cut(s) 1632
FalI AAGNNNNNCTT 2 cut(s) 1126, 1158
FaqI GGGAC 2 cut(s) 427, 1486
FauNDI CATATG 2 cut(s) 177, 722
FbaI TGATCA 4 cut(s) 214, 487, 996, 1089
Fnu4HI GCNGC 7 cut(s) 48, 924, 945, 1064, 1116, 1242, 1605
FokI GGATG 4 cut(s) 90, 529, 1608, 1714
FriOI GRGCYC 2 cut(s) 111, 980
Fsp4HI GCNGC 7 cut(s) 48, 924, 945, 1064, 1116, 1242, 1605
FspAI RTGCGCAY 1 cut(s) 318
FspBI CTAG 6 cut(s) 270, 342, 831, 1221, 1383, 1686
FspI TGCGCA 3 cut(s) 287, 318, 668
GlaI GCGC 7 cut(s) 6, 8, 257, 287, 318, 668, 1641
GluI GCNGC 7 cut(s) 48, 924, 945, 1064, 1116, 1242, 1605
GsaI CCCAGC 1 cut(s) 846
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 5 cut(s) 182, 231, 358, 926, 1208
HapII CCGG 9 cut(s) 237, 429, 560, 819, 849, 1038, 1077, 1275, 1722
HgaI GACGC 1 cut(s) 373
HhaI GCGC 7 cut(s) 7, 9, 258, 288, 319, 669, 1642
Hin6I GCGC 7 cut(s) 5, 7, 256, 286, 317, 667, 1640
HinP1I GCGC 7 cut(s) 5, 7, 256, 286, 317, 667, 1640
HinfI GANTC 7 cut(s) 302, 506, 555, 761, 766, 1421, 1543
HpaII CCGG 9 cut(s) 237, 429, 560, 819, 849, 1038, 1077, 1275, 1722
HphI GGTGA 2 cut(s) 182, 1591
Hpy166II GTNNAC 6 cut(s) 276, 434, 1043, 1405, 1500, 1521
Hpy188I TCNGA 4 cut(s) 363, 453, 729, 1548
Hpy8I GTNNAC 6 cut(s) 276, 434, 1043, 1405, 1500, 1521
HpyCH4III ACNGT 4 cut(s) 202, 758, 1402, 1666
HpyCH4IV ACGT 3 cut(s) 294, 789, 1523
HpyCH4V TGCA 9 cut(s) 67, 98, 119, 175, 424, 1118, 1133, 1569, 1607
HpyF10VI GCNNNNNNNGC 9 cut(s) 95, 253, 264, 314, 364, 932, 941, 1053, 1139
HpyF3I CTNAG 9 cut(s) 90, 304, 353, 551, 1195, 1331, 1556, 1562, 1695
HpySE526I ACGT 3 cut(s) 294, 789, 1523
HspAI GCGC 7 cut(s) 5, 7, 256, 286, 317, 667, 1640
Kpn2I TCCGGA 1 cut(s) 1721
KroI GCCGGC 1 cut(s) 1274
KroNI GCCGGC 1 cut(s) 1276
Ksp22I TGATCA 4 cut(s) 214, 487, 996, 1089
Kzo9I GATC 7 cut(s) 214, 487, 529, 996, 1089, 1585, 1707
LmnI GCTCC 8 cut(s) 106, 392, 851, 932, 1100, 1327, 1516, 1624
Lsp1109I GCAGC 4 cut(s) 34, 1050, 1102, 1591
MaeI CTAG 6 cut(s) 270, 342, 831, 1221, 1383, 1686
MaeII ACGT 3 cut(s) 294, 789, 1523
MaeIII GTNAC 7 cut(s) 344, 516, 758, 1004, 1280, 1396, 1524
MalI GATC 7 cut(s) 216, 489, 531, 998, 1091, 1587, 1709
MboI GATC 7 cut(s) 214, 487, 529, 996, 1089, 1585, 1707
MboII GAAGA 5 cut(s) 49, 311, 453, 603, 999
MflI RGATCY 1 cut(s) 1707
MhlI GDGCHC 5 cut(s) 111, 243, 389, 980, 1203
MluCI AATT 6 cut(s) 34, 611, 634, 1215, 1477, 1680
MlyI GAGTC 3 cut(s) 296, 549, 755
MmeI TCCRAC 2 cut(s) 1103, 1593
Mph1103I ATGCAT 1 cut(s) 177
MroI TCCGGA 1 cut(s) 1721
MroNI GCCGGC 1 cut(s) 1274
MroXI GAANNNNTTC 2 cut(s) 1141, 1729
MseI TTAA 5 cut(s) 32, 206, 774, 1052, 1671
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 1646
MspCI CTTAAG 1 cut(s) 1051
MspI CCGG 9 cut(s) 237, 429, 560, 819, 849, 1038, 1077, 1275, 1722
MspR9I CCNGG 4 cut(s) 238, 560, 819, 1038
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 9 cut(s) 95, 253, 264, 314, 364, 932, 941, 1053, 1139
NaeI GCCGGC 1 cut(s) 1276
NciI CCSGG 4 cut(s) 238, 560, 819, 1038
NcoI CCATGG 1 cut(s) 262
NdeI CATATG 2 cut(s) 177, 722
NdeII GATC 7 cut(s) 214, 487, 529, 996, 1089, 1585, 1707
NgoMIV GCCGGC 1 cut(s) 1274
NlaIV GGNNCC 4 cut(s) 108, 242, 1620, 1709
NmeAIII GCCGAG 1 cut(s) 1217
NmuCI GTSAC 3 cut(s) 516, 758, 1524
NsbI TGCGCA 3 cut(s) 287, 318, 668
NsiI ATGCAT 1 cut(s) 177
NspI RCATGY 5 cut(s) 98, 312, 1036, 1063, 1660
NspV TTCGAA 2 cut(s) 250, 1305
OliI CACNNNNGTG 1 cut(s) 161
PaeI GCATGC 2 cut(s) 98, 312
PaeR7I CTCGAG 1 cut(s) 1148
PauI GCGCGC 1 cut(s) 5
PceI AGGCCT 1 cut(s) 358
PcsI WCGNNNNNNNCGW 1 cut(s) 1529
PdiI GCCGGC 1 cut(s) 1276
PdmI GAANNNNTTC 2 cut(s) 1141, 1729
PfeI GAWTC 4 cut(s) 506, 766, 1421, 1543
PfoI TCCNGGA 1 cut(s) 558
PinAI ACCGGT 1 cut(s) 428
PkrI GCNGC 7 cut(s) 49, 925, 946, 1065, 1117, 1243, 1606
PleI GAGTC 3 cut(s) 296, 549, 755
PpsI GAGTC 3 cut(s) 296, 549, 755
Psp124BI GAGCTC 1 cut(s) 980
PspFI CCCAGC 1 cut(s) 842
PspN4I GGNNCC 4 cut(s) 108, 242, 1620, 1709
PspPI GGNCC 3 cut(s) 181, 230, 1040
PspXI VCTCGAGB 1 cut(s) 1148
PsrI GAACNNNNNNTAC 2 cut(s) 1013, 1045
PstI CTGCAG 2 cut(s) 1120, 1609
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 1 cut(s) 1707
PteI GCGCGC 1 cut(s) 5
PvuII CAGCTG 1 cut(s) 1646
RsaI GTAC 8 cut(s) 82, 277, 332, 803, 962, 1031, 1298, 1404
RsaNI GTAC 8 cut(s) 81, 276, 331, 802, 961, 1030, 1297, 1403
RseI CAYNNNNRTG 1 cut(s) 161
SacI GAGCTC 1 cut(s) 980
SaqAI TTAA 5 cut(s) 32, 206, 774, 1052, 1671
SatI GCNGC 7 cut(s) 48, 924, 945, 1064, 1116, 1242, 1605
Sau3AI GATC 7 cut(s) 214, 487, 529, 996, 1089, 1585, 1707
Sau96I GGNCC 3 cut(s) 181, 230, 1040
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 3 cut(s) 296, 549, 755
ScrFI CCNGG 4 cut(s) 238, 560, 819, 1038
SduI GDGCHC 5 cut(s) 111, 243, 389, 980, 1203
SfcI CTRYAG 2 cut(s) 1116, 1605
Sfr274I CTCGAG 1 cut(s) 1148
SfuI TTCGAA 2 cut(s) 250, 1305
SinI GGWCC 1 cut(s) 1040
SlaI CTCGAG 1 cut(s) 1148
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 5 cut(s) 403, 973, 1051, 1148, 1202
SmoI CTYRAG 5 cut(s) 403, 973, 1051, 1148, 1202
SphI GCATGC 2 cut(s) 98, 312
Sse9I AATT 6 cut(s) 34, 611, 634, 1215, 1477, 1680
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 6 cut(s) 193, 915, 923, 945, 1241, 1514
SspMI CTAG 6 cut(s) 270, 342, 831, 1221, 1383, 1686
SstI GAGCTC 1 cut(s) 980
StuI AGGCCT 1 cut(s) 358
StyD4I CCNGG 4 cut(s) 236, 558, 817, 1036
StyI CCWWGG 2 cut(s) 262, 1169
TaaI ACNGT 4 cut(s) 202, 758, 1402, 1666
TaiI ACGT 3 cut(s) 297, 792, 1526
TaqII GACCGA 1 cut(s) 847
TasI AATT 6 cut(s) 34, 611, 634, 1215, 1477, 1680
TatI WGTACW 4 cut(s) 80, 275, 801, 1029
TauI GCSGC 3 cut(s) 926, 947, 1244
TfiI GAWTC 4 cut(s) 506, 766, 1421, 1543
Tru1I TTAA 5 cut(s) 32, 206, 774, 1052, 1671
Tru9I TTAA 5 cut(s) 32, 206, 774, 1052, 1671
TscAI CASTG 8 cut(s) 163, 497, 525, 1203, 1507, 1614, 1669, 1703
TseFI GTSAC 3 cut(s) 516, 758, 1524
TseI GCWGC 4 cut(s) 47, 1063, 1115, 1604
Tsp45I GTSAC 3 cut(s) 516, 758, 1524
TspDTI ATGAA 4 cut(s) 531, 624, 733, 1556
TspGWI ACGGA 2 cut(s) 1340, 1614
TspRI CASTG 8 cut(s) 163, 497, 525, 1203, 1507, 1614, 1669, 1703
Vha464I CTTAAG 1 cut(s) 1051
VpaK11BI GGWCC 1 cut(s) 1040
XapI RAATTY 4 cut(s) 34, 611, 1215, 1680
XbaI TCTAGA 1 cut(s) 1382
XceI RCATGY 5 cut(s) 98, 312, 1036, 1063, 1660
XhoI CTCGAG 1 cut(s) 1148
XmnI GAANNNNTTC 2 cut(s) 1141, 1729
XspI CTAG 6 cut(s) 270, 342, 831, 1221, 1383, 1686
ZrmI AGTACT 1 cut(s) 803
Zsp2I ATGCAT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.