Rroxscaffold_3G00263320

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000761
Physical Location & Seq
Forward (+)
57287384 .. 57290382
2999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263320.1

Sequence Viewer

Length: 1137 bp
ATGGGTGGTCAAGCTTCAACATCCAGAAGTTCTAGGTGGTCTCGGCCACCTCCACCTCAAACCCATGGGCCTCTTTCTCGGTCTGGGCAACAGCAGCATGTTGCACCTCCACCTCTGCCGAGGCCGACGCCGACACCACAACCCCCGTTGCGGCCCCTGCCTCCACCTGAAAGCTATGAGGTTTCTTCTGTAGATTGGAATTATCCACCAGTTTATGATGTTTACCCTGATGAAGACGACGAGCCAGTGGGTAATGAGGTCACGGATCGATTGAAGCGTGCTGGTCTGGAGTCTACCAAACTTATTTTTGCTTTTGATTTCACAAAGAGCAATGAGCAGAGAGGTGCAAGGTCATTCAACGGGAAGAGCTTGCATTACATTGCAGCAGAGCGAAATCCCTATGAACAAGCAGTATCGATCATTGGGAAAACATTGGACTACTTCGATGAGGATGGGAAGATTCCCTGTTTTGGATTTGGAGATGCATCAACCGCTGACCAGGAAGTTTTCAGTTTCTATGAAGATAAGAGTGCTTGCTGCATTGGATTTGAAGAAGTAATAGAACGATATAGAGAAGTAGTCCCCCAACTACAACTTGGAGGGCCTAAATCACTTGCCCCCATCATTGAAATGGCCACAACTATTGTTGGACAGAGTGGTGGCCAGTACCATGTATTGGTGATACTAGCTGATGGGCAGGTGACTAGACCACAGGAAAAGAAAACAGTTGAAGCAATTGTGAAAGCAAGCGAGTATCCCTTATCAATTGTGGTAGTTGGAGTTGGAGATGGGCCATGGGATATGATGAAGAAATTTGAGAGTAAAATCCTTCCACGGAACTTTTTCGTGAATGCTGCAGACATTTTGTCAAAGAATATGGACAGCTCAAGAAAAGAAGAAGAATTTGCTTCTGCAGCCTTGCTCGAATTACCCTCTCAGTATAAAGCAATACAAGAGCTTGGCATATTGGGAGAGAGAATGCGGTGGGCTGGATTCCTCTTCCCCCTCCTCGTTATGGTTCAGGCTCTGATGGCAGTGACAGAGCACATCATGCCTCTGACAATCATACATGCTGTGACTGTGGACCACATCTTGAGAAGTGCCCGATTTGCAGAAGCGCCATCGACCTCAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

378

Amino Acids

41.81

Weight (kDa)

5.25

Isoelectric Point (pI)

58.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Copine PF07002 123 - 323 1.6e-60 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 688
Acc36I ACCTGC 1 cut(s) 688
AccB7I CCANNNNNTGG 1 cut(s) 676
AccI GTMKAC 1 cut(s) 293
AciI CCGC 3 cut(s) 151, 492, 982
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 3 cut(s) 44, 633, 661
AcsI RAATTY 2 cut(s) 812, 902
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 1 cut(s) 668
AfiI CCNNNNNNNGG 4 cut(s) 150, 470, 676, 1015
AgsI TTSAA 6 cut(s) 18, 274, 358, 551, 629, 731
AhdI GACNNNNNGTC 1 cut(s) 865
AjnI CCWGG 1 cut(s) 498
AluBI AGCT 6 cut(s) 14, 174, 369, 689, 885, 958
AluI AGCT 6 cut(s) 14, 174, 369, 689, 885, 958
Alw21I GWGCWC 1 cut(s) 1047
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 1 cut(s) 273
AlwNI CAGNNNCTG 1 cut(s) 1027
AoxI GGCC 8 cut(s) 44, 68, 122, 152, 602, 633, 661, 791
ApeKI GCWGC 5 cut(s) 94, 383, 537, 854, 914
ApoI RAATTY 2 cut(s) 812, 902
Asp700I GAANNNNTTC 1 cut(s) 842
AspLEI GCGC 1 cut(s) 1120
AspS9I GGNCC 5 cut(s) 68, 153, 602, 791, 1084
AsuHPI GGTGA 2 cut(s) 691, 712
AvaII GGWCC 1 cut(s) 1084
AxyI CCTNAGG 1 cut(s) 1129
BaeGI GKGCMC 1 cut(s) 1105
BalI TGGCCA 2 cut(s) 635, 663
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 1047
BbvI GCAGC 5 cut(s) 106, 395, 524, 841, 926
BccI CCATC 6 cut(s) 446, 629, 686, 782, 1024, 1129
BciT130I CCWGG 1 cut(s) 500
BciVI GTATCC 1 cut(s) 765
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 3 cut(s) 33, 686, 705
BfmI CTRYAG 3 cut(s) 189, 855, 912
BfoI RGCGCY 1 cut(s) 1121
BfuAI ACCTGC 1 cut(s) 688
BfuI GTATCC 1 cut(s) 765
BisI GCNGC 6 cut(s) 95, 152, 384, 538, 855, 915
BlsI GCNGC 6 cut(s) 96, 153, 385, 539, 856, 916
Bme1390I CCNGG 1 cut(s) 500
Bme18I GGWCC 1 cut(s) 1084
BmeRI GACNNNNNGTC 1 cut(s) 865
BmgT120I GGNCC 5 cut(s) 68, 153, 602, 791, 1084
BmiI GGNNCC 1 cut(s) 155
BmrFI CCNGG 1 cut(s) 500
BmsI GCATC 2 cut(s) 472, 494
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 308
BpuEI CTTGAG 2 cut(s) 871, 1114
Bsa29I ATCGAT 2 cut(s) 268, 416
BsaHI GRCGYC 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 4 cut(s) 64, 119, 794, 833
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 470, 676, 1015
Bse1I ACTGG 3 cut(s) 209, 245, 664
Bse21I CCTNAGG 1 cut(s) 1129
Bse3DI GCAATG 2 cut(s) 337, 378
BseBI CCWGG 1 cut(s) 500
BseCI ATCGAT 2 cut(s) 268, 416
BseDI CCNNGG 4 cut(s) 64, 119, 794, 833
BseGI GGATG 2 cut(s) 20, 457
BseLI CCNNNNNNNGG 4 cut(s) 150, 470, 676, 1015
BseMI GCAATG 2 cut(s) 337, 378
BseMII CTCAG 1 cut(s) 950
BseNI ACTGG 3 cut(s) 209, 245, 664
BseRI GAGGAG 1 cut(s) 998
BseSI GKGCMC 1 cut(s) 1105
BseXI GCAGC 5 cut(s) 106, 395, 524, 841, 926
BshFI GGCC 8 cut(s) 46, 70, 124, 154, 604, 635, 663, 793
BshVI ATCGAT 2 cut(s) 268, 416
BsiHKAI GWGCWC 1 cut(s) 1047
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 4 cut(s) 150, 470, 676, 1015
BsmAI GTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 566
BsmI GAATGC 2 cut(s) 856, 984
BsnI GGCC 8 cut(s) 46, 70, 124, 154, 604, 635, 663, 793
Bso31I GGTCTC 1 cut(s) 45
Bsp1286I GDGCHC 2 cut(s) 1047, 1105
Bsp143I GATC 2 cut(s) 265, 417
Bsp19I CCATGG 2 cut(s) 64, 794
BspACI CCGC 3 cut(s) 151, 492, 982
BspANI GGCC 8 cut(s) 46, 70, 124, 154, 604, 635, 663, 793
BspCNI CTCAG 1 cut(s) 949
BspDI ATCGAT 2 cut(s) 268, 416
BspLI GGNNCC 1 cut(s) 155
BspMAI CTGCAG 2 cut(s) 859, 916
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 1 cut(s) 273
BspQI GCTCTTC 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 45
BsrDI GCAATG 2 cut(s) 337, 378
BsrI ACTGG 3 cut(s) 209, 245, 664
BssECI CCNNGG 4 cut(s) 64, 119, 794, 833
BssMI GATC 2 cut(s) 265, 417
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 2 cut(s) 64, 794
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 2 cut(s) 727, 1081
Bst6I CTCTTC 2 cut(s) 359, 1004
BstACI GRCGYC 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 1051
BstC8I GCNNGC 4 cut(s) 279, 371, 535, 748
BstDEI CTNAG 2 cut(s) 936, 1129
BstDSI CCRYGG 3 cut(s) 64, 794, 833
BstF5I GGATG 2 cut(s) 20, 457
BstH2I RGCGCY 1 cut(s) 1121
BstHHI GCGC 1 cut(s) 1120
BstKTI GATC 2 cut(s) 268, 420
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 2 cut(s) 265, 417
BstMWI GCNNNNNNNGC 7 cut(s) 94, 157, 491, 914, 1031, 1051, 1109
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 2 cut(s) 101, 1073
BstSCI CCNGG 1 cut(s) 498
BstSFI CTRYAG 3 cut(s) 189, 855, 912
BstSLI GKGCMC 1 cut(s) 1105
BstV1I GCAGC 5 cut(s) 106, 395, 524, 841, 926
BstV2I GAAGAC 1 cut(s) 240
Bsu15I ATCGAT 2 cut(s) 268, 416
Bsu36I CCTNAGG 1 cut(s) 1129
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 8 cut(s) 46, 70, 124, 154, 604, 635, 663, 793
BsuTUI ATCGAT 2 cut(s) 268, 416
BtgI CCRYGG 3 cut(s) 64, 794, 833
BtsCI GGATG 2 cut(s) 20, 457
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 2 cut(s) 252, 1041
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 4 cut(s) 279, 371, 535, 748
CaiI CAGNNNCTG 1 cut(s) 1027
CfoI GCGC 1 cut(s) 1120
Cfr13I GGNCC 5 cut(s) 68, 153, 602, 791, 1084
ClaI ATCGAT 2 cut(s) 268, 416
CseI GACGC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 667
CspCI CAANNNNNGTGG 2 cut(s) 625, 660
CviAII CATG 6 cut(s) 65, 98, 671, 795, 1051, 1070
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 2 cut(s) 936, 1129
DpnI GATC 2 cut(s) 267, 419
DpnII GATC 2 cut(s) 265, 417
DriI GACNNNNNGTC 1 cut(s) 865
EaeI YGGCCR 3 cut(s) 44, 633, 661
Eam1104I CTCTTC 2 cut(s) 359, 1004
Eam1105I GACNNNNNGTC 1 cut(s) 865
EarI CTCTTC 2 cut(s) 359, 1004
Eco130I CCWWGG 2 cut(s) 64, 794
Eco31I GGTCTC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 1084
Eco81I CCTNAGG 1 cut(s) 1129
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 498
EcoT14I CCWWGG 2 cut(s) 64, 794
EcoT22I ATGCAT 1 cut(s) 487
ErhI CCWWGG 2 cut(s) 64, 794
FaeI CATG 6 cut(s) 68, 101, 674, 798, 1054, 1073
FaqI GGGAC 1 cut(s) 566
FatI CATG 6 cut(s) 64, 97, 670, 794, 1050, 1069
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 6 cut(s) 95, 152, 384, 538, 855, 915
FokI GGATG 2 cut(s) 7, 464
Fsp4HI GCNGC 6 cut(s) 95, 152, 384, 538, 855, 915
FspBI CTAG 3 cut(s) 33, 686, 705
GlaI GCGC 1 cut(s) 1119
GluI GCNGC 6 cut(s) 95, 152, 384, 538, 855, 915
GsuI CTGGAG 1 cut(s) 308
HaeII RGCGCY 1 cut(s) 1121
HaeIII GGCC 8 cut(s) 46, 70, 124, 154, 604, 635, 663, 793
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 1120
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 6 cut(s) 68, 101, 674, 798, 1054, 1073
Hin6I GCGC 1 cut(s) 1118
HinP1I GCGC 1 cut(s) 1118
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 3 cut(s) 290, 460, 993
HphI GGTGA 2 cut(s) 691, 712
Hpy166II GTNNAC 3 cut(s) 223, 294, 1084
Hpy188I TCNGA 2 cut(s) 1029, 1059
Hpy188III TCNNGA 6 cut(s) 24, 287, 847, 888, 1093, 1131
Hpy8I GTNNAC 3 cut(s) 223, 294, 1084
Hpy99I CGWCG 2 cut(s) 130, 242
HpyAV CCTTC 1 cut(s) 839
HpyCH4III ACNGT 2 cut(s) 727, 1081
HpyCH4V TGCA 9 cut(s) 104, 347, 373, 383, 485, 540, 857, 914, 1112
HpyF10VI GCNNNNNNNGC 7 cut(s) 94, 157, 491, 914, 1031, 1051, 1109
HpyF3I CTNAG 2 cut(s) 936, 1129
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 6 cut(s) 68, 101, 674, 798, 1054, 1073
HspAI GCGC 1 cut(s) 1118
Kzo9I GATC 2 cut(s) 265, 417
LguI GCTCTTC 1 cut(s) 359
Lsp1109I GCAGC 5 cut(s) 106, 395, 524, 841, 926
LweI GCATC 2 cut(s) 472, 494
MaeI CTAG 3 cut(s) 33, 686, 705
MaeIII GTNAC 4 cut(s) 259, 700, 1036, 1075
MalI GATC 2 cut(s) 267, 419
MboI GATC 2 cut(s) 265, 417
MfeI CAATTG 2 cut(s) 735, 765
MhlI GDGCHC 2 cut(s) 1047, 1105
MlsI TGGCCA 2 cut(s) 635, 663
MluCI AATT 6 cut(s) 199, 735, 765, 812, 902, 926
MluNI TGGCCA 2 cut(s) 635, 663
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 3 cut(s) 628, 757, 763
Mox20I TGGCCA 2 cut(s) 635, 663
Mph1103I ATGCAT 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 842
MscI TGGCCA 2 cut(s) 635, 663
MslI CAYNNNNRTG 1 cut(s) 629
Msp20I TGGCCA 2 cut(s) 635, 663
MspA1I CMGCKG 1 cut(s) 494
MspR9I CCNGG 1 cut(s) 500
MunI CAATTG 2 cut(s) 735, 765
Mva1269I GAATGC 2 cut(s) 856, 984
MvaI CCWGG 1 cut(s) 500
MwoI GCNNNNNNNGC 7 cut(s) 94, 157, 491, 914, 1031, 1051, 1109
NcoI CCATGG 2 cut(s) 64, 794
NdeII GATC 2 cut(s) 265, 417
NlaIII CATG 6 cut(s) 68, 101, 674, 798, 1054, 1073
NlaIV GGNNCC 1 cut(s) 155
NmeAIII GCCGAG 2 cut(s) 22, 144
NmuCI GTSAC 4 cut(s) 259, 700, 1036, 1075
NsiI ATGCAT 1 cut(s) 487
NspI RCATGY 2 cut(s) 101, 1073
PaqCI CACCTGC 1 cut(s) 688
PciSI GCTCTTC 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 274
PctI GAATGC 2 cut(s) 856, 984
PdmI GAANNNNTTC 1 cut(s) 842
PfeI GAWTC 2 cut(s) 460, 993
PflMI CCANNNNNTGG 1 cut(s) 676
PkrI GCNGC 6 cut(s) 96, 153, 385, 539, 856, 916
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 498
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 1 cut(s) 155
PspPI GGNCC 5 cut(s) 68, 153, 602, 791, 1084
PstI CTGCAG 2 cut(s) 859, 916
PstNI CAGNNNCTG 1 cut(s) 1027
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
RseI CAYNNNNRTG 1 cut(s) 629
SapI GCTCTTC 1 cut(s) 359
SatI GCNGC 6 cut(s) 95, 152, 384, 538, 855, 915
Sau3AI GATC 2 cut(s) 265, 417
Sau96I GGNCC 5 cut(s) 68, 153, 602, 791, 1084
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 500
SduI GDGCHC 2 cut(s) 1047, 1105
SfaNI GCATC 2 cut(s) 472, 494
SfcI CTRYAG 3 cut(s) 189, 855, 912
SinI GGWCC 1 cut(s) 1084
SmiMI CAYNNNNRTG 1 cut(s) 629
SmlI CTYRAG 2 cut(s) 886, 1093
SmoI CTYRAG 2 cut(s) 886, 1093
Sse9I AATT 6 cut(s) 199, 735, 765, 812, 902, 926
SsiI CCGC 3 cut(s) 151, 492, 982
SspMI CTAG 3 cut(s) 33, 686, 705
StyD4I CCNGG 1 cut(s) 498
StyI CCWWGG 2 cut(s) 64, 794
TaaI ACNGT 2 cut(s) 727, 1081
TaqI TCGA 5 cut(s) 268, 416, 444, 924, 1124
TaqII GACCGA 1 cut(s) 69
TasI AATT 6 cut(s) 199, 735, 765, 812, 902, 926
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 2 cut(s) 460, 993
TscAI CASTG 2 cut(s) 252, 1041
TseFI GTSAC 4 cut(s) 259, 700, 1036, 1075
TseI GCWGC 5 cut(s) 94, 383, 537, 854, 914
Tsp45I GTSAC 4 cut(s) 259, 700, 1036, 1075
TspDTI ATGAA 4 cut(s) 246, 417, 534, 821
TspGWI ACGGA 2 cut(s) 278, 850
TspRI CASTG 2 cut(s) 252, 1041
Van91I CCANNNNNTGG 1 cut(s) 676
VpaK11BI GGWCC 1 cut(s) 1084
XapI RAATTY 2 cut(s) 812, 902
XceI RCATGY 2 cut(s) 101, 1073
XcmI CCANNNNNNNNNTGG 2 cut(s) 593, 628
XmiI GTMKAC 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 842
XspI CTAG 3 cut(s) 33, 686, 705
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.