Rh7BG124800

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000761
Physical Location & Seq
Reverse (-)
9279509 .. 9282869
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG124800.1

Sequence Viewer

Length: 1341 bp
ATGGGTTGTCAAGCTTCAACATTCAGATTTCCTAGTAGAAGGTCTCGTCGGTTTGCGCCACGACGGTTTCCACCTCCAGCTCAAAGCCATAGGGCGCTTCATGCTCAGTCTAGGCAACAGCAGCGTGTTGCACCTCCGCCTCCACCTCCACATCCTCTTCCACTTCCACCTCCACCTCCGTCGCTCCCGCCCCTGTCTCCACCTAAAAGCTATGAGTTGGAGAACAAATACTCAAATACAGATGCTAATCTGGTCAGCCAGCTAGTTCCTGATGTAGATTGGAATTCTCCAGCAGTTTCTGATGTTTACCTTGATCAAGATGACAAGCCTGAGGGTAATGAGGTCAAGGAACAATATTTGAAGCATGCCGGTCTGGAGTCTGCCAAACTTATTTTTGGTATTGATTTCTCACAGAGCAATGAGCGGAGAGGTGCAAGGTCCTGTAACGGGAAGAGCTTGCATTACATTGGAGCAGAGCAAAATCCCTATGAACAAGCAATATCGATCACTGGGAAAATATTGCAGTACTTTGATAAGGATACGATGATTCCCTGTTTTGGATTTGGAGATGCATCAACCAATGACCAGGAAGTTTTCAGTTTCTATCCAGATGAGAGTGCTTGCTGCAATGGATTTGAAGAAGCAACAAAACGATATAGAGAAGTAGTCCCCCAGCTACAACTTGGAGGGCCTAAATCATTTGCCCCTATCATTGAAATGGCCATGACTCTTGTTGAACAGAGTGGTGGCCAGTACCATGTGTTGGTGATACTAGCTGATGGGCAGGTGCCTAGAGGAGAAGATACTGCTCTTAGCCAGTTAAGCCCACAGGAAAAGAAAACAGTTGAAGCAATTGTGAAAGCAAGCGAGTATCCCTTATCAATTGTGGTAGTTGGAGTTGGAGATGGACCATGGGATATGATGGAGAAATTTGAGGGTAAAATTCCTCGACGGGCCTTTGATAACTTTCGGTTTGTGAATGCTGCAGACATTTTCTCAAAGAATATGGACAGCTCAAGAAAAGATGTAAAATTTGCTTCTGCAGCCTTGCTGCAATTACCCTCTCAGTATAAAGCAACACAAGAGCTTAGCATATTGGGTGCTACAAGAGAGAATGCGGTGCGTAGGATTCCTCTTCCCCCTCCTCGTTACAGTTCAGGCTTTGTAGGCAGTGAACGAGCACCTCACGCTTCTGACAATCATCTCTGTCCCGTTTGCCTAACCAATGAGAAAAATATGGCATTTGGTTGCGGACATCAGACATGCTGTGACTGTGGACCAGATCTTGAGACGTGCCCGATTTGCAGAAGCGCCATCTACCTCAGGATAAAACTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

446

Amino Acids

49.47

Weight (kDa)

6.52

Isoelectric Point (pI)

57.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Copine PF07002 152 - 366 5.7e-63 Copine
zf-C3HC4_3 PF13920 400 - 439 4.6e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 775
Acc36I ACCTGC 1 cut(s) 775
AccB1I GGYRCC 1 cut(s) 787
AccB7I CCANNNNNTGG 1 cut(s) 763
AccBSI CCGCTC 1 cut(s) 424
AciI CCGC 5 cut(s) 137, 188, 424, 1118, 1251
AcoI YGGCCR 2 cut(s) 720, 748
AcsI RAATTY 4 cut(s) 283, 929, 942, 1031
AfaI GTAC 2 cut(s) 527, 755
AfiI CCNNNNNNNGG 3 cut(s) 447, 557, 763
AgsI TTSAA 6 cut(s) 18, 361, 638, 716, 737, 848
AjiI CACGTC 1 cut(s) 1293
AjnI CCWGG 1 cut(s) 585
AluBI AGCT 9 cut(s) 14, 80, 210, 262, 456, 676, 776, 1014, 1087
AluI AGCT 9 cut(s) 14, 80, 210, 262, 456, 676, 776, 1014, 1087
Alw21I GWGCWC 1 cut(s) 1183
Alw26I GTCTC 3 cut(s) 48, 201, 1283
AlwNI CAGNNNCTG 1 cut(s) 299
AoxI GGCC 4 cut(s) 689, 720, 748, 954
ApeKI GCWGC 5 cut(s) 121, 624, 983, 1043, 1051
ApoI RAATTY 4 cut(s) 283, 929, 942, 1031
AspLEI GCGC 3 cut(s) 58, 97, 1313
AspS9I GGNCC 5 cut(s) 438, 689, 908, 954, 1277
AsuHPI GGTGA 1 cut(s) 778
AvaII GGWCC 3 cut(s) 438, 908, 1277
AxyI CCTNAGG 2 cut(s) 330, 1322
BaeGI GKGCMC 1 cut(s) 1298
BalI TGGCCA 2 cut(s) 722, 750
BanI GGYRCC 1 cut(s) 787
Bbv12I GWGCWC 1 cut(s) 1183
BbvI GCAGC 5 cut(s) 133, 611, 970, 1038, 1055
BccI CCATC 4 cut(s) 773, 899, 916, 1322
BciT130I CCWGG 1 cut(s) 587
BciVI GTATCC 2 cut(s) 532, 882
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 3 cut(s) 48, 201, 1283
BfaI CTAG 5 cut(s) 33, 111, 263, 773, 792
BfmI CTRYAG 2 cut(s) 984, 1041
BfoI RGCGCY 2 cut(s) 98, 1314
BfuAI ACCTGC 1 cut(s) 775
BfuI GTATCC 2 cut(s) 532, 882
BglII AGATCT 1 cut(s) 1282
BisI GCNGC 5 cut(s) 122, 625, 984, 1044, 1052
BlpI GCTNAGC 1 cut(s) 1088
BlsI GCNGC 5 cut(s) 123, 626, 985, 1045, 1053
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 1 cut(s) 587
Bme18I GGWCC 3 cut(s) 438, 908, 1277
BmgBI CACGTC 1 cut(s) 1293
BmgT120I GGNCC 5 cut(s) 438, 689, 908, 954, 1277
BmiI GGNNCC 1 cut(s) 789
BmrFI CCNGG 1 cut(s) 587
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 3 cut(s) 232, 559, 581
BmuI ACTGGG 1 cut(s) 519
BpmI CTGGAG 3 cut(s) 60, 273, 395
Bpu1102I GCTNAGC 1 cut(s) 1088
BpuEI CTTGAG 2 cut(s) 1000, 1307
Bsa29I ATCGAT 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 911
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 557, 763
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 3 cut(s) 514, 751, 817
Bse21I CCTNAGG 2 cut(s) 330, 1322
Bse3DI GCAATG 2 cut(s) 424, 634
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 1 cut(s) 587
BseCI ATCGAT 1 cut(s) 503
BseDI CCNNGG 1 cut(s) 911
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 3 cut(s) 447, 557, 763
BseMI GCAATG 2 cut(s) 424, 634
BseMII CTCAG 4 cut(s) 119, 321, 1079, 1336
BseNI ACTGG 3 cut(s) 514, 751, 817
BseRI GAGGAG 2 cut(s) 810, 1134
BseSI GKGCMC 1 cut(s) 1298
BseXI GCAGC 5 cut(s) 133, 611, 970, 1038, 1055
BseYI CCCAGC 1 cut(s) 672
BshFI GGCC 4 cut(s) 691, 722, 750, 956
BshNI GGYRCC 1 cut(s) 787
BshVI ATCGAT 1 cut(s) 503
BsiHKAI GWGCWC 1 cut(s) 1183
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 2 cut(s) 653, 1194
BslI CCNNNNNNNGG 3 cut(s) 447, 557, 763
BsmAI GTCTC 3 cut(s) 48, 201, 1283
BsmBI CGTCTC 1 cut(s) 1283
BsmFI GGGAC 2 cut(s) 653, 1194
BsmI GAATGC 2 cut(s) 985, 1120
BsnI GGCC 4 cut(s) 691, 722, 750, 956
Bso31I GGTCTC 1 cut(s) 48
Bsp1286I GDGCHC 2 cut(s) 1183, 1298
Bsp143I GATC 3 cut(s) 313, 504, 1282
Bsp1720I GCTNAGC 1 cut(s) 1088
Bsp19I CCATGG 1 cut(s) 911
BspACI CCGC 5 cut(s) 137, 188, 424, 1118, 1251
BspANI GGCC 4 cut(s) 691, 722, 750, 956
BspCNI CTCAG 4 cut(s) 118, 322, 1078, 1335
BspDI ATCGAT 1 cut(s) 503
BspLI GGNNCC 1 cut(s) 789
BspMAI CTGCAG 2 cut(s) 988, 1045
BspMI ACCTGC 1 cut(s) 775
BspQI GCTCTTC 1 cut(s) 446
BspT107I GGYRCC 1 cut(s) 787
BspTNI GGTCTC 1 cut(s) 48
BsrBI CCGCTC 1 cut(s) 424
BsrDI GCAATG 2 cut(s) 424, 634
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 3 cut(s) 514, 751, 817
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 1 cut(s) 911
BssMI GATC 3 cut(s) 313, 504, 1282
BssT1I CCWWGG 1 cut(s) 911
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 4 cut(s) 66, 844, 1154, 1274
Bst6I CTCTTC 3 cut(s) 162, 446, 1140
BstC8I GCNNGC 5 cut(s) 260, 366, 458, 622, 865
BstDEI CTNAG 6 cut(s) 105, 330, 812, 1065, 1088, 1322
BstDSI CCRYGG 1 cut(s) 911
BstF5I GGATG 1 cut(s) 151
BstH2I RGCGCY 2 cut(s) 98, 1314
BstHHI GCGC 3 cut(s) 58, 97, 1313
BstKTI GATC 3 cut(s) 316, 507, 1285
BstMAI GTCTC 3 cut(s) 48, 201, 1283
BstMBI GATC 3 cut(s) 313, 504, 1282
BstMWI GCNNNNNNNGC 7 cut(s) 101, 121, 822, 1043, 1167, 1187, 1302
BstNI CCWGG 1 cut(s) 587
BstNSI RCATGY 2 cut(s) 368, 1266
BstSCI CCNGG 1 cut(s) 585
BstSFI CTRYAG 2 cut(s) 984, 1041
BstSLI GKGCMC 1 cut(s) 1298
BstV1I GCAGC 5 cut(s) 133, 611, 970, 1038, 1055
BstX2I RGATCY 1 cut(s) 1282
BstYI RGATCY 1 cut(s) 1282
Bsu15I ATCGAT 1 cut(s) 503
Bsu36I CCTNAGG 2 cut(s) 330, 1322
BsuI GTATCC 2 cut(s) 532, 882
BsuRI GGCC 4 cut(s) 691, 722, 750, 956
BsuTUI ATCGAT 1 cut(s) 503
BtgI CCRYGG 1 cut(s) 911
BtrI CACGTC 1 cut(s) 1293
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 1 cut(s) 1177
BtsIMutI CAGTG 2 cut(s) 507, 1177
BveI ACCTGC 1 cut(s) 775
Cac8I GCNNGC 5 cut(s) 260, 366, 458, 622, 865
CaiI CAGNNNCTG 1 cut(s) 299
CfoI GCGC 3 cut(s) 58, 97, 1313
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 5 cut(s) 438, 689, 908, 954, 1277
ClaI ATCGAT 1 cut(s) 503
Csp6I GTAC 2 cut(s) 526, 754
CviAII CATG 6 cut(s) 101, 365, 724, 758, 912, 1263
CviQI GTAC 2 cut(s) 526, 754
DdeI CTNAG 6 cut(s) 105, 330, 812, 1065, 1088, 1322
DpnI GATC 3 cut(s) 315, 506, 1284
DpnII GATC 3 cut(s) 313, 504, 1282
EaeI YGGCCR 2 cut(s) 720, 748
Eam1104I CTCTTC 3 cut(s) 162, 446, 1140
EarI CTCTTC 3 cut(s) 162, 446, 1140
EciI GGCGGA 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 911
Eco31I GGTCTC 1 cut(s) 48
Eco47I GGWCC 3 cut(s) 438, 908, 1277
Eco81I CCTNAGG 2 cut(s) 330, 1322
EcoO109I RGGNCCY 2 cut(s) 438, 689
EcoRI GAATTC 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 585
EcoT14I CCWWGG 1 cut(s) 911
EcoT22I ATGCAT 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 911
Esp3I CGTCTC 1 cut(s) 1283
FaeI CATG 6 cut(s) 104, 368, 727, 761, 915, 1266
FaqI GGGAC 2 cut(s) 653, 1194
FatI CATG 6 cut(s) 100, 364, 723, 757, 911, 1262
FauI CCCGC 1 cut(s) 195
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 5 cut(s) 122, 625, 984, 1044, 1052
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 5 cut(s) 122, 625, 984, 1044, 1052
FspBI CTAG 5 cut(s) 33, 111, 263, 773, 792
GlaI GCGC 3 cut(s) 57, 96, 1312
GluI GCNGC 5 cut(s) 122, 625, 984, 1044, 1052
GsaI CCCAGC 1 cut(s) 676
GsuI CTGGAG 3 cut(s) 60, 273, 395
HaeII RGCGCY 2 cut(s) 98, 1314
HaeIII GGCC 4 cut(s) 691, 722, 750, 956
HapII CCGG 1 cut(s) 369
HhaI GCGC 3 cut(s) 58, 97, 1313
Hin1II CATG 6 cut(s) 104, 368, 727, 761, 915, 1266
Hin6I GCGC 3 cut(s) 56, 95, 1311
HinP1I GCGC 3 cut(s) 56, 95, 1311
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 4 cut(s) 377, 547, 727, 1129
HpaII CCGG 1 cut(s) 369
HphI GGTGA 1 cut(s) 778
Hpy166II GTNNAC 3 cut(s) 307, 1175, 1277
Hpy188I TCNGA 4 cut(s) 26, 301, 1195, 1260
Hpy188III TCNNGA 7 cut(s) 269, 317, 374, 608, 1017, 1286, 1324
Hpy8I GTNNAC 3 cut(s) 307, 1175, 1277
Hpy99I CGWCG 4 cut(s) 51, 66, 184, 954
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 4 cut(s) 66, 844, 1154, 1274
HpyCH4IV ACGT 1 cut(s) 1292
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 121, 822, 1043, 1167, 1187, 1302
HpyF3I CTNAG 6 cut(s) 105, 330, 812, 1065, 1088, 1322
HpySE526I ACGT 1 cut(s) 1292
Hsp92II CATG 6 cut(s) 104, 368, 727, 761, 915, 1266
HspAI GCGC 3 cut(s) 56, 95, 1311
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 3 cut(s) 313, 504, 1282
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 2 cut(s) 189, 470
Lsp1109I GCAGC 5 cut(s) 133, 611, 970, 1038, 1055
LweI GCATC 3 cut(s) 232, 559, 581
MaeI CTAG 5 cut(s) 33, 111, 263, 773, 792
MaeII ACGT 1 cut(s) 1292
MaeIII GTNAC 3 cut(s) 443, 1148, 1268
MalI GATC 3 cut(s) 315, 506, 1284
MbiI CCGCTC 1 cut(s) 424
MboI GATC 3 cut(s) 313, 504, 1282
MboII GAAGA 5 cut(s) 149, 463, 650, 812, 1127
MfeI CAATTG 2 cut(s) 852, 882
MflI RGATCY 1 cut(s) 1282
MhlI GDGCHC 2 cut(s) 1183, 1298
MlsI TGGCCA 2 cut(s) 722, 750
MluCI AATT 7 cut(s) 283, 852, 882, 929, 942, 1031, 1055
MluNI TGGCCA 2 cut(s) 722, 750
MlyI GAGTC 2 cut(s) 386, 721
MmeI TCCRAC 3 cut(s) 198, 874, 880
Mox20I TGGCCA 2 cut(s) 722, 750
Mph1103I ATGCAT 1 cut(s) 574
MscI TGGCCA 2 cut(s) 722, 750
MseI TTAA 1 cut(s) 821
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 2 cut(s) 722, 750
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 1 cut(s) 587
MunI CAATTG 2 cut(s) 852, 882
Mva1269I GAATGC 2 cut(s) 985, 1120
MvaI CCWGG 1 cut(s) 587
MwoI GCNNNNNNNGC 7 cut(s) 101, 121, 822, 1043, 1167, 1187, 1302
NcoI CCATGG 1 cut(s) 911
NdeII GATC 3 cut(s) 313, 504, 1282
NlaIII CATG 6 cut(s) 104, 368, 727, 761, 915, 1266
NlaIV GGNNCC 1 cut(s) 789
NmuCI GTSAC 1 cut(s) 1268
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 2 cut(s) 368, 1266
PaeI GCATGC 1 cut(s) 368
PaqCI CACCTGC 1 cut(s) 775
PciSI GCTCTTC 1 cut(s) 446
PctI GAATGC 2 cut(s) 985, 1120
PfeI GAWTC 2 cut(s) 547, 1129
PflMI CCANNNNNTGG 1 cut(s) 763
PkrI GCNGC 5 cut(s) 123, 626, 985, 1045, 1053
PleI GAGTC 2 cut(s) 385, 721
PpsI GAGTC 2 cut(s) 385, 721
PpuMI RGGWCCY 1 cut(s) 438
Psp5II RGGWCCY 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 585
PspFI CCCAGC 1 cut(s) 672
PspGI CCWGG 1 cut(s) 585
PspN4I GGNNCC 1 cut(s) 789
PspPI GGNCC 5 cut(s) 438, 689, 908, 954, 1277
PspPPI RGGWCCY 1 cut(s) 438
PstI CTGCAG 2 cut(s) 988, 1045
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 1 cut(s) 1282
RsaI GTAC 2 cut(s) 527, 755
RsaNI GTAC 2 cut(s) 526, 754
RseI CAYNNNNRTG 1 cut(s) 716
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 1 cut(s) 821
SatI GCNGC 5 cut(s) 122, 625, 984, 1044, 1052
Sau3AI GATC 3 cut(s) 313, 504, 1282
Sau96I GGNCC 5 cut(s) 438, 689, 908, 954, 1277
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 2 cut(s) 386, 721
ScrFI CCNGG 1 cut(s) 587
SduI GDGCHC 2 cut(s) 1183, 1298
SfaNI GCATC 3 cut(s) 232, 559, 581
SfcI CTRYAG 2 cut(s) 984, 1041
SinI GGWCC 3 cut(s) 438, 908, 1277
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 2 cut(s) 1015, 1286
SmoI CTYRAG 2 cut(s) 1015, 1286
SphI GCATGC 1 cut(s) 368
Sse9I AATT 7 cut(s) 283, 852, 882, 929, 942, 1031, 1055
SsiI CCGC 5 cut(s) 137, 188, 424, 1118, 1251
SspI AATATT 2 cut(s) 356, 519
SspMI CTAG 5 cut(s) 33, 111, 263, 773, 792
StyD4I CCNGG 1 cut(s) 585
StyI CCWWGG 1 cut(s) 911
TaaI ACNGT 4 cut(s) 66, 844, 1154, 1274
TaiI ACGT 1 cut(s) 1295
TaqI TCGA 2 cut(s) 503, 949
TasI AATT 7 cut(s) 283, 852, 882, 929, 942, 1031, 1055
TatI WGTACW 1 cut(s) 525
TfiI GAWTC 2 cut(s) 547, 1129
Tru1I TTAA 1 cut(s) 821
Tru9I TTAA 1 cut(s) 821
TscAI CASTG 2 cut(s) 514, 1177
TseFI GTSAC 1 cut(s) 1268
TseI GCWGC 5 cut(s) 121, 624, 983, 1043, 1051
Tsp45I GTSAC 1 cut(s) 1268
TspDTI ATGAA 2 cut(s) 89, 504
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 2 cut(s) 514, 1177
Van91I CCANNNNNTGG 1 cut(s) 763
VpaK11BI GGWCC 3 cut(s) 438, 908, 1277
XapI RAATTY 4 cut(s) 283, 929, 942, 1031
XceI RCATGY 2 cut(s) 368, 1266
XcmI CCANNNNNNNNNTGG 1 cut(s) 680
XspI CTAG 5 cut(s) 33, 111, 263, 773, 792
ZrmI AGTACT 1 cut(s) 527
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.