RLG00000004404

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000761
Physical Location & Seq
Forward (+)
59093647 .. 59096480
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004404

Sequence Viewer

Length: 1356 bp
ATGGGTTGTCAAGCTTCAACATTCAGATTTCCTAGTAGAAGGTCTCGTCGGTTTGCTCCACGACGGTTTCCACCTCCAGCTCAAAGCCATAGGGCGCTTCATGCTCAGTCTGGGCAACAGCAGCGTGTTGCACCTCCGCCTCCACCTCCACATCCTCTTCCACTTCCACCTCCACCCCCGTCGCTCCCGCCCCTGTCTTCACCTAAAAGCTATGAGTTGGAGAACAAATACTCAAATACAGATGCTAATCTGGTCTGCCAGCTGGTTCCTGATGTAGATTGGAATTCTCCAGCAGTTTCTGATGTCTACCTTGATCAAGATGTCAAGCCTGTGGGTAATGAGGTCACGGATCAATATTTGAAGCATGCCGGTCTGGAGTCTGCCAAACTTATTTTTGGTATTGATTTCTCACAGAGCAATGAGCGGAGAGGTGCAAGGTCCTGTAACGGGAAGAGCTTGCATTACATTGGAGCAGAGCAAAATCCCTATGAACAAGCAATATTGATCACTGGGAAAATATTGCAGTACTTTGATAAGGATACGATGATTCCCTGTTTTGGATTTGGAGATGTTAATTCATTCAAGGCATCAACAAATGACCAGGAAGTTTTCAGTTTCTATCCAGATGAGAGTGCTTGCTGCAATGGATTTGAAGAAGCAACAAAACGATATAGAGAAGTAGTCCCCCAGCTACAACTTGGAGGGCCTAAATCATTTGCCCCTATCATTGAAATGGCCATGACTCTTGTTGAACAGAGTGGTGGCCAGTACCATGTGTTGGTGATACTAGCTGATGGGCAGGTGATTAGAGGAGAAGATACTGCTCTTCGCCAGTTAAGCCCACAGGAAAAGAAAACAGTTGAAGCAATTGTGAAAGCCAGCGAGTATCCCTTATCAATTGTGGTAGTTGGAGTGGGAGATGGACCATGGGATATGATGGAGAAATTTGAGGGTAAAATTCCTCGACGGGCCTTTGATAACTTTCGGTTTGTGAATGCTGCAGACATTTTCTCAAAGAATATGGACAGCTCAAGAAGAGATGTAAAATTTGCTTCTGCAGCCTTGCTGCAATTACCCTCTCAGTATAAAGCAACACAAGAGCTTAGCATATTGGGTGCTACAAGAGAGAATGCGGTGCGCAGGATTCCTCTTCCCCCTCCTCGTTACAGTTCAGGCTTTGTTGGCAGTGAAAGAGCACCACATGCTTCTGACAATCATCTCTGTCCCGTTTGCCTAACATTGGAGAAAAATATGGCATTTGGTTGCGGACATCAGACATGCTGTGACTGTGGACCAGATCTTGAGACGTGCCCGATTTGCAGAAGCGCCATCTACCTCAGGATAAAACTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

452

Amino Acids

50.0

Weight (kDa)

7.08

Isoelectric Point (pI)

57.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Copine PF07002 152 - 371 1.7e-63 Copine
zf-C3HC4_3 PF13920 405 - 444 3.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 790
Acc16I TGCGCA 1 cut(s) 1139
Acc36I ACCTGC 1 cut(s) 790
AccB7I CCANNNNNTGG 1 cut(s) 778
AccBSI CCGCTC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 306
AciI CCGC 5 cut(s) 137, 188, 424, 1133, 1266
AclWI GGATC 1 cut(s) 357
AcoI YGGCCR 2 cut(s) 735, 763
AcsI RAATTY 4 cut(s) 283, 944, 957, 1046
AfaI GTAC 2 cut(s) 527, 770
AfiI CCNNNNNNNGG 4 cut(s) 447, 557, 778, 1240
AgsI TTSAA 7 cut(s) 18, 361, 583, 653, 731, 752, 863
AjiI CACGTC 1 cut(s) 1308
AjnI CCWGG 1 cut(s) 600
AluBI AGCT 9 cut(s) 14, 80, 210, 262, 456, 691, 791, 1029, 1102
AluI AGCT 9 cut(s) 14, 80, 210, 262, 456, 691, 791, 1029, 1102
Alw21I GWGCWC 1 cut(s) 1198
Alw26I GTCTC 2 cut(s) 48, 1298
AlwI GGATC 1 cut(s) 357
AlwNI CAGNNNCTG 1 cut(s) 299
AoxI GGCC 4 cut(s) 704, 735, 763, 969
ApeKI GCWGC 5 cut(s) 121, 639, 998, 1058, 1066
ApoI RAATTY 4 cut(s) 283, 944, 957, 1046
AspLEI GCGC 3 cut(s) 97, 1140, 1328
AspS9I GGNCC 5 cut(s) 438, 704, 923, 969, 1292
AsuHPI GGTGA 3 cut(s) 192, 793, 814
AvaII GGWCC 3 cut(s) 438, 923, 1292
AxyI CCTNAGG 1 cut(s) 1337
BaeGI GKGCMC 1 cut(s) 1313
BalI TGGCCA 2 cut(s) 737, 765
BbsI GAAGAC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 1198
BbvI GCAGC 5 cut(s) 133, 626, 985, 1053, 1070
BccI CCATC 4 cut(s) 788, 914, 931, 1337
BciT130I CCWGG 1 cut(s) 602
BciVI GTATCC 2 cut(s) 532, 897
BclI TGATCA 2 cut(s) 313, 504
BcoDI GTCTC 2 cut(s) 48, 1298
BfaI CTAG 2 cut(s) 33, 788
BfmI CTRYAG 2 cut(s) 999, 1056
BfoI RGCGCY 2 cut(s) 98, 1329
BfuAI ACCTGC 1 cut(s) 790
BfuI GTATCC 2 cut(s) 532, 897
BglII AGATCT 1 cut(s) 1297
BisI GCNGC 5 cut(s) 122, 640, 999, 1059, 1067
BlpI GCTNAGC 1 cut(s) 1103
BlsI GCNGC 5 cut(s) 123, 641, 1000, 1060, 1068
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 1 cut(s) 602
Bme18I GGWCC 3 cut(s) 438, 923, 1292
BmgBI CACGTC 1 cut(s) 1308
BmgT120I GGNCC 5 cut(s) 438, 704, 923, 969, 1292
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 602
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 2 cut(s) 232, 596
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 189
BpmI CTGGAG 3 cut(s) 60, 273, 395
Bpu1102I GCTNAGC 1 cut(s) 1103
BpuEI CTTGAG 2 cut(s) 1015, 1322
BsaBI GATNNNNATC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 926
Bsc4I CCNNNNNNNGG 4 cut(s) 447, 557, 778, 1240
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 3 cut(s) 514, 766, 832
Bse21I CCTNAGG 1 cut(s) 1337
Bse3DI GCAATG 2 cut(s) 424, 649
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 1 cut(s) 926
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 4 cut(s) 447, 557, 778, 1240
BseMI GCAATG 2 cut(s) 424, 649
BseMII CTCAG 3 cut(s) 119, 1094, 1351
BseNI ACTGG 3 cut(s) 514, 766, 832
BseRI GAGGAG 2 cut(s) 825, 1149
BseSI GKGCMC 1 cut(s) 1313
BseXI GCAGC 5 cut(s) 133, 626, 985, 1053, 1070
BseYI CCCAGC 1 cut(s) 687
BshFI GGCC 4 cut(s) 706, 737, 765, 971
BsiHKAI GWGCWC 1 cut(s) 1198
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 2 cut(s) 668, 1209
BslI CCNNNNNNNGG 4 cut(s) 447, 557, 778, 1240
BsmAI GTCTC 2 cut(s) 48, 1298
BsmBI CGTCTC 1 cut(s) 1298
BsmFI GGGAC 2 cut(s) 668, 1209
BsmI GAATGC 2 cut(s) 1000, 1135
BsnI GGCC 4 cut(s) 706, 737, 765, 971
Bso31I GGTCTC 1 cut(s) 48
Bsp1286I GDGCHC 2 cut(s) 1198, 1313
Bsp143I GATC 4 cut(s) 313, 349, 504, 1297
Bsp1720I GCTNAGC 1 cut(s) 1103
Bsp19I CCATGG 1 cut(s) 926
BspACI CCGC 5 cut(s) 137, 188, 424, 1133, 1266
BspANI GGCC 4 cut(s) 706, 737, 765, 971
BspCNI CTCAG 3 cut(s) 118, 1093, 1350
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 2 cut(s) 1003, 1060
BspMI ACCTGC 1 cut(s) 790
BspPI GGATC 1 cut(s) 357
BspQI GCTCTTC 2 cut(s) 446, 831
BspTNI GGTCTC 1 cut(s) 48
BsrBI CCGCTC 1 cut(s) 424
BsrDI GCAATG 2 cut(s) 424, 649
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 3 cut(s) 514, 766, 832
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 1 cut(s) 926
BssMI GATC 4 cut(s) 313, 349, 504, 1297
BssT1I CCWWGG 1 cut(s) 926
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 4 cut(s) 66, 859, 1169, 1289
Bst6I CTCTTC 5 cut(s) 162, 446, 831, 1030, 1155
BstAPI GCANNNNNTGC 1 cut(s) 1202
BstC8I GCNNGC 5 cut(s) 260, 366, 458, 637, 880
BstDEI CTNAG 4 cut(s) 105, 1080, 1103, 1337
BstDSI CCRYGG 1 cut(s) 926
BstF5I GGATG 1 cut(s) 151
BstH2I RGCGCY 2 cut(s) 98, 1329
BstHHI GCGC 3 cut(s) 97, 1140, 1328
BstKTI GATC 4 cut(s) 316, 352, 507, 1300
BstMAI GTCTC 2 cut(s) 48, 1298
BstMBI GATC 4 cut(s) 313, 349, 504, 1297
BstMWI GCNNNNNNNGC 7 cut(s) 101, 121, 837, 1058, 1182, 1202, 1317
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 3 cut(s) 368, 1205, 1281
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 2 cut(s) 999, 1056
BstSLI GKGCMC 1 cut(s) 1313
BstV1I GCAGC 5 cut(s) 133, 626, 985, 1053, 1070
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 1297
BstYI RGATCY 1 cut(s) 1297
Bsu36I CCTNAGG 1 cut(s) 1337
BsuI GTATCC 2 cut(s) 532, 897
BsuRI GGCC 4 cut(s) 706, 737, 765, 971
BtgI CCRYGG 1 cut(s) 926
BtrI CACGTC 1 cut(s) 1308
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 1 cut(s) 1192
BtsIMutI CAGTG 2 cut(s) 507, 1192
BveI ACCTGC 1 cut(s) 790
Cac8I GCNNGC 5 cut(s) 260, 366, 458, 637, 880
CaiI CAGNNNCTG 1 cut(s) 299
CfoI GCGC 3 cut(s) 97, 1140, 1328
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 5 cut(s) 438, 704, 923, 969, 1292
Csp6I GTAC 2 cut(s) 526, 769
CviAII CATG 7 cut(s) 101, 365, 739, 773, 927, 1202, 1278
CviQI GTAC 2 cut(s) 526, 769
DdeI CTNAG 4 cut(s) 105, 1080, 1103, 1337
DpnI GATC 4 cut(s) 315, 351, 506, 1299
DpnII GATC 4 cut(s) 313, 349, 504, 1297
EaeI YGGCCR 2 cut(s) 735, 763
Eam1104I CTCTTC 5 cut(s) 162, 446, 831, 1030, 1155
EarI CTCTTC 5 cut(s) 162, 446, 831, 1030, 1155
EciI GGCGGA 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 926
Eco31I GGTCTC 1 cut(s) 48
Eco47I GGWCC 3 cut(s) 438, 923, 1292
Eco81I CCTNAGG 1 cut(s) 1337
EcoO109I RGGNCCY 2 cut(s) 438, 704
EcoRI GAATTC 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 926
ErhI CCWWGG 1 cut(s) 926
Esp3I CGTCTC 1 cut(s) 1298
FaeI CATG 7 cut(s) 104, 368, 742, 776, 930, 1205, 1281
FaqI GGGAC 2 cut(s) 668, 1209
FatI CATG 7 cut(s) 100, 364, 738, 772, 926, 1201, 1277
FauI CCCGC 1 cut(s) 195
FbaI TGATCA 2 cut(s) 313, 504
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 5 cut(s) 122, 640, 999, 1059, 1067
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 5 cut(s) 122, 640, 999, 1059, 1067
FspBI CTAG 2 cut(s) 33, 788
FspI TGCGCA 1 cut(s) 1139
GlaI GCGC 3 cut(s) 96, 1139, 1327
GluI GCNGC 5 cut(s) 122, 640, 999, 1059, 1067
GsaI CCCAGC 1 cut(s) 691
GsuI CTGGAG 3 cut(s) 60, 273, 395
HaeII RGCGCY 2 cut(s) 98, 1329
HaeIII GGCC 4 cut(s) 706, 737, 765, 971
HapII CCGG 1 cut(s) 369
HhaI GCGC 3 cut(s) 97, 1140, 1328
Hin1II CATG 7 cut(s) 104, 368, 742, 776, 930, 1205, 1281
Hin6I GCGC 3 cut(s) 95, 1138, 1326
HinP1I GCGC 3 cut(s) 95, 1138, 1326
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 4 cut(s) 377, 547, 742, 1144
HpaII CCGG 1 cut(s) 369
HphI GGTGA 3 cut(s) 192, 793, 814
Hpy166II GTNNAC 2 cut(s) 307, 1292
Hpy188I TCNGA 4 cut(s) 26, 301, 1210, 1275
Hpy188III TCNNGA 7 cut(s) 269, 317, 374, 623, 1032, 1301, 1339
Hpy8I GTNNAC 2 cut(s) 307, 1292
Hpy99I CGWCG 4 cut(s) 51, 66, 184, 969
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 4 cut(s) 66, 859, 1169, 1289
HpyCH4IV ACGT 1 cut(s) 1307
HpyCH4V TGCA 9 cut(s) 131, 434, 460, 523, 642, 1001, 1058, 1069, 1320
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 121, 837, 1058, 1182, 1202, 1317
HpyF3I CTNAG 4 cut(s) 105, 1080, 1103, 1337
HpySE526I ACGT 1 cut(s) 1307
Hsp92II CATG 7 cut(s) 104, 368, 742, 776, 930, 1205, 1281
HspAI GCGC 3 cut(s) 95, 1138, 1326
Ksp22I TGATCA 2 cut(s) 313, 504
Kzo9I GATC 4 cut(s) 313, 349, 504, 1297
LguI GCTCTTC 2 cut(s) 446, 831
LmnI GCTCC 3 cut(s) 61, 189, 470
Lsp1109I GCAGC 5 cut(s) 133, 626, 985, 1053, 1070
LweI GCATC 2 cut(s) 232, 596
MaeI CTAG 2 cut(s) 33, 788
MaeII ACGT 1 cut(s) 1307
MaeIII GTNAC 4 cut(s) 343, 443, 1163, 1283
MalI GATC 4 cut(s) 315, 351, 506, 1299
MbiI CCGCTC 1 cut(s) 424
MboI GATC 4 cut(s) 313, 349, 504, 1297
MboII GAAGA 8 cut(s) 149, 189, 463, 665, 818, 827, 1047, 1142
MfeI CAATTG 2 cut(s) 867, 897
MflI RGATCY 1 cut(s) 1297
MhlI GDGCHC 2 cut(s) 1198, 1313
MlsI TGGCCA 2 cut(s) 737, 765
MluCI AATT 8 cut(s) 283, 574, 867, 897, 944, 957, 1046, 1070
MluNI TGGCCA 2 cut(s) 737, 765
MlyI GAGTC 2 cut(s) 386, 736
MmeI TCCRAC 2 cut(s) 198, 889
Mox20I TGGCCA 2 cut(s) 737, 765
MscI TGGCCA 2 cut(s) 737, 765
MseI TTAA 2 cut(s) 573, 836
MslI CAYNNNNRTG 1 cut(s) 731
Msp20I TGGCCA 2 cut(s) 737, 765
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 1 cut(s) 602
MunI CAATTG 2 cut(s) 867, 897
Mva1269I GAATGC 2 cut(s) 1000, 1135
MvaI CCWGG 1 cut(s) 602
MwoI GCNNNNNNNGC 7 cut(s) 101, 121, 837, 1058, 1182, 1202, 1317
NcoI CCATGG 1 cut(s) 926
NdeII GATC 4 cut(s) 313, 349, 504, 1297
NlaIII CATG 7 cut(s) 104, 368, 742, 776, 930, 1205, 1281
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 2 cut(s) 343, 1283
NsbI TGCGCA 1 cut(s) 1139
NspI RCATGY 3 cut(s) 368, 1205, 1281
PaeI GCATGC 1 cut(s) 368
PaqCI CACCTGC 1 cut(s) 790
PciSI GCTCTTC 2 cut(s) 446, 831
PctI GAATGC 2 cut(s) 1000, 1135
PfeI GAWTC 2 cut(s) 547, 1144
PflMI CCANNNNNTGG 1 cut(s) 778
PkrI GCNGC 5 cut(s) 123, 641, 1000, 1060, 1068
PleI GAGTC 2 cut(s) 385, 736
PpsI GAGTC 2 cut(s) 385, 736
PpuMI RGGWCCY 1 cut(s) 438
Psp5II RGGWCCY 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 687
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 5 cut(s) 438, 704, 923, 969, 1292
PspPPI RGGWCCY 1 cut(s) 438
PstI CTGCAG 2 cut(s) 1003, 1060
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 1 cut(s) 1297
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 2 cut(s) 527, 770
RsaNI GTAC 2 cut(s) 526, 769
RseI CAYNNNNRTG 1 cut(s) 731
SapI GCTCTTC 2 cut(s) 446, 831
SaqAI TTAA 2 cut(s) 573, 836
SatI GCNGC 5 cut(s) 122, 640, 999, 1059, 1067
Sau3AI GATC 4 cut(s) 313, 349, 504, 1297
Sau96I GGNCC 5 cut(s) 438, 704, 923, 969, 1292
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 2 cut(s) 386, 736
ScrFI CCNGG 1 cut(s) 602
SduI GDGCHC 2 cut(s) 1198, 1313
SfaNI GCATC 2 cut(s) 232, 596
SfcI CTRYAG 2 cut(s) 999, 1056
SinI GGWCC 3 cut(s) 438, 923, 1292
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 2 cut(s) 1030, 1301
SmoI CTYRAG 2 cut(s) 1030, 1301
SphI GCATGC 1 cut(s) 368
Sse9I AATT 8 cut(s) 283, 574, 867, 897, 944, 957, 1046, 1070
SsiI CCGC 5 cut(s) 137, 188, 424, 1133, 1266
SspI AATATT 3 cut(s) 356, 501, 519
SspMI CTAG 2 cut(s) 33, 788
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 1 cut(s) 926
TaaI ACNGT 4 cut(s) 66, 859, 1169, 1289
TaiI ACGT 1 cut(s) 1310
TaqI TCGA 1 cut(s) 964
TasI AATT 8 cut(s) 283, 574, 867, 897, 944, 957, 1046, 1070
TatI WGTACW 1 cut(s) 525
TfiI GAWTC 2 cut(s) 547, 1144
Tru1I TTAA 2 cut(s) 573, 836
Tru9I TTAA 2 cut(s) 573, 836
TscAI CASTG 2 cut(s) 514, 1192
TseFI GTSAC 2 cut(s) 343, 1283
TseI GCWGC 5 cut(s) 121, 639, 998, 1058, 1066
Tsp45I GTSAC 2 cut(s) 343, 1283
TspDTI ATGAA 3 cut(s) 89, 504, 567
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 2 cut(s) 514, 1192
Van91I CCANNNNNTGG 1 cut(s) 778
VpaK11BI GGWCC 3 cut(s) 438, 923, 1292
XapI RAATTY 4 cut(s) 283, 944, 957, 1046
XceI RCATGY 3 cut(s) 368, 1205, 1281
XcmI CCANNNNNNNNNTGG 1 cut(s) 695
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 2 cut(s) 33, 788
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.