Prupe.5G163100_v2.0.a1

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000761
Physical Location & Seq
Reverse (-)
14245670 .. 14249855
4186 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G163100.2

Sequence Viewer

Length: 1323 bp
ATGGGTGGGATAATTTCGAAACGTGCTACTTCGAGACAGGCTTCTTTTGGGTCTGGATCGCATTCTTGGAGTCAACAAAGTTATCCAGAGGTACCATATGCACAACCATATGTACCACCAAGCCAAGACTATGGGCAACAGCAGCATTTTGCACCACCACCTCAAAGCCACGGTAGTGCTTGGCCAGGGTCGAAAAAGAGGTTGGAAAGGAAGTATTCAAAAATAGATGATGATTACAACAGCCTGGAGCAGGTCACAGATGCATTGGCACGTGCTGGTTTGGAGTCATCCAATCTTATTGTGGGCATTGATTTCACAAAGAGCAACGAGTGGACAGGTGCAAGGTCATTTCATCGGAAAAGCTTGCATCACATTGGAGAAGAGCAAAATCCCTATGAACAGGCAATATCAATTATAGGGAAAACATTGTCTTCCTTTGATGAAGATAACTTGATTCCCTGTTTTGGATTTGGAGATGCATCAACCCATGACCAAGAAGTTTTCAGTTTCTACCCAGATGAGGGATCTTTTTGCAATGGATTTGAAGAAGTATTAAGACGATATAGAGAATTAGTCCCTCAGCTACGACTTGCCGGGCCCACATCATTTGCCCCTATTATTGAAATGGCCATTACTATTGTTGAGCAAAGTGGTGGTCAGTACCATGTGTTGGTGATAATAGCTGATGGACAGGTGACCAGGAGTGTTGATACAGAGCGCGGCCAGTTAAGCCCACAGGAAAGGAAAACTGTTGAAGCAATTGTGAAAGCAAGTGAGTATCCCTTATCAATTATACTAGTTGGAGTTGGAGATGGGCCGTGGGATATGATGAAGGAATTTGATGATAACATCCCCGCTCGGGCCTTCGATAACTTTCAGTTTGTGAATTTTACAGAAATTATGACAAAGAATATGGATCGTTCGAGAAAAGAAGCAGAATTTGCTCTTGCAGCATTGATGGAAATACCCTCCCAGTATAAAGCAACCCTCGAGCTTAATATATTAGGTTCTACAAGAGGGAAGGCTGTAGATAGGGTACCTCTTCCCCCGCCTCATTATGGTTCGGCTTCTTTTAGCATGCCAAAACCGTCACAATCAAGTAATTTTCGTCCAAGTGCACCATCCAGGCAACGTGATGAGTTTGTTAGCACAGCACGTCCTGCAAGTTCAGCTTCTGATAATCATGTCTGTCCCATTTGCCTAACCGATCCGAAGAATATGGCATTTGGTTGTGGACATCAGACATGCTGTGACTGTGGACAAGATCTTCAATTATGCCCCATTTGCAGAAGCACCATTGAAACCCGAATAAAGCTCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

441

Amino Acids

48.86

Weight (kDa)

5.62

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 241
Acc65I GGTACC 2 cut(s) 91, 1036
AccB1I GGYRCC 2 cut(s) 91, 1036
AccB7I CCANNNNNTGG 1 cut(s) 670
AccBSI CCGCTC 1 cut(s) 857
AccII CGCG 1 cut(s) 720
AciI CCGC 3 cut(s) 720, 855, 1049
AclWI GGATC 4 cut(s) 64, 532, 924, 1202
AcoI YGGCCR 3 cut(s) 182, 627, 721
AcsI RAATTY 3 cut(s) 836, 886, 938
AcvI CACGTG 1 cut(s) 272
AfaI GTAC 4 cut(s) 93, 114, 662, 1038
AfiI CCNNNNNNNGG 7 cut(s) 250, 464, 520, 521, 670, 859, 1058
AgsI TTSAA 6 cut(s) 219, 545, 623, 755, 1271, 1301
AhlI ACTAGT 1 cut(s) 796
AjiI CACGTC 1 cut(s) 1157
AjnI CCWGG 4 cut(s) 184, 243, 698, 1124
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AleI CACNNNNGTG 1 cut(s) 174
AluBI AGCT 6 cut(s) 363, 583, 683, 994, 1172, 1315
AluI AGCT 6 cut(s) 363, 583, 683, 994, 1172, 1315
Alw21I GWGCWC 1 cut(s) 1120
Alw26I GTCTC 1 cut(s) 28
Alw44I GTGCAC 1 cut(s) 1116
AlwI GGATC 4 cut(s) 64, 532, 924, 1202
AlwNI CAGNNNCTG 1 cut(s) 1175
Ama87I CYCGRG 2 cut(s) 858, 989
AoxI GGCC 6 cut(s) 182, 596, 627, 721, 815, 861
ApaI GGGCCC 1 cut(s) 600
ApaLI GTGCAC 1 cut(s) 1116
ApeKI GCWGC 2 cut(s) 142, 950
ApoI RAATTY 3 cut(s) 836, 886, 938
ArsI GACNNNNNNTTYG 2 cut(s) 640, 672
Asp718I GGTACC 2 cut(s) 91, 1036
AspLEI GCGC 1 cut(s) 720
AspS9I GGNCC 4 cut(s) 596, 597, 815, 861
AsuC2I CCSGG 1 cut(s) 595
AsuHPI GGTGA 2 cut(s) 685, 706
AsuII TTCGAA 1 cut(s) 17
AvaI CYCGRG 2 cut(s) 858, 989
BaeGI GKGCMC 2 cut(s) 600, 1120
BalI TGGCCA 2 cut(s) 184, 629
BanI GGYRCC 2 cut(s) 91, 1036
BanII GRGCYC 1 cut(s) 600
BbrPI CACGTG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 1120
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 2 cut(s) 154, 962
BccI CCATC 4 cut(s) 680, 806, 952, 1129
BceAI ACGGC 1 cut(s) 802
BciT130I CCWGG 4 cut(s) 186, 245, 700, 1126
BciVI GTATCC 1 cut(s) 789
BcnI CCSGG 1 cut(s) 595
BcoDI GTCTC 1 cut(s) 28
BcuI ACTAGT 1 cut(s) 796
BfaI CTAG 1 cut(s) 797
BfmI CTRYAG 1 cut(s) 1026
BfuAI ACCTGC 1 cut(s) 241
BfuI GTATCC 1 cut(s) 789
BglII AGATCT 1 cut(s) 1264
BisI GCNGC 3 cut(s) 143, 721, 951
BlsI GCNGC 3 cut(s) 144, 722, 952
Bme1390I CCNGG 5 cut(s) 186, 245, 595, 700, 1126
BmeT110I CYCGRG 2 cut(s) 858, 989
BmgBI CACGTC 1 cut(s) 1157
BmgT120I GGNCC 4 cut(s) 596, 597, 815, 861
BmiI GGNNCC 3 cut(s) 93, 598, 1038
BmrFI CCNGG 5 cut(s) 186, 245, 595, 700, 1126
BmrI ACTGGG 1 cut(s) 967
BmsI GCATC 4 cut(s) 250, 376, 466, 488
BmuI ACTGGG 1 cut(s) 967
BpiI GAAGAC 1 cut(s) 423
BpmI CTGGAG 1 cut(s) 266
Bpu10I CCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 272
BsaJI CCNNGG 3 cut(s) 169, 185, 818
Bsc4I CCNNNNNNNGG 7 cut(s) 250, 464, 520, 521, 670, 859, 1058
Bse1I ACTGG 2 cut(s) 724, 973
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 4 cut(s) 186, 245, 700, 1126
BseDI CCNNGG 3 cut(s) 169, 185, 818
BseGI GGATG 3 cut(s) 287, 849, 1121
BseLI CCNNNNNNNGG 7 cut(s) 250, 464, 520, 521, 670, 859, 1058
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 1 cut(s) 593
BseNI ACTGG 2 cut(s) 724, 973
BseSI GKGCMC 2 cut(s) 600, 1120
BseXI GCAGC 2 cut(s) 154, 962
Bsh1236I CGCG 1 cut(s) 720
BshFI GGCC 6 cut(s) 184, 598, 629, 723, 817, 863
BshNI GGYRCC 2 cut(s) 91, 1036
BsiHKAI GWGCWC 1 cut(s) 1120
BsiHKCI CYCGRG 2 cut(s) 858, 989
BsiSI CCGG 1 cut(s) 594
BslFI GGGAC 2 cut(s) 560, 1176
BslI CCNNNNNNNGG 7 cut(s) 250, 464, 520, 521, 670, 859, 1058
BsmAI GTCTC 1 cut(s) 28
BsmFI GGGAC 2 cut(s) 560, 1176
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 6 cut(s) 184, 598, 629, 723, 817, 863
BsoBI CYCGRG 2 cut(s) 858, 989
Bsp119I TTCGAA 1 cut(s) 17
Bsp120I GGGCCC 1 cut(s) 596
Bsp1286I GDGCHC 2 cut(s) 600, 1120
Bsp143I GATC 5 cut(s) 56, 524, 916, 1207, 1264
BspACI CCGC 3 cut(s) 720, 855, 1049
BspANI GGCC 6 cut(s) 184, 598, 629, 723, 817, 863
BspCNI CTCAG 1 cut(s) 592
BspFNI CGCG 1 cut(s) 720
BspLI GGNNCC 3 cut(s) 93, 598, 1038
BspMI ACCTGC 1 cut(s) 241
BspPI GGATC 4 cut(s) 64, 532, 924, 1202
BspQI GCTCTTC 1 cut(s) 375
BspT104I TTCGAA 1 cut(s) 17
BspT107I GGYRCC 2 cut(s) 91, 1036
BsrBI CCGCTC 1 cut(s) 857
BsrDI GCAATG 1 cut(s) 541
BsrI ACTGG 2 cut(s) 724, 973
BssECI CCNNGG 3 cut(s) 169, 185, 818
BssMI GATC 5 cut(s) 56, 524, 916, 1207, 1264
Bst2UI CCWGG 4 cut(s) 186, 245, 700, 1126
Bst4CI ACNGT 4 cut(s) 173, 751, 1089, 1256
Bst6I CTCTTC 2 cut(s) 375, 1047
BstAPI GCANNNNNTGC 2 cut(s) 941, 1160
BstBAI YACGTR 1 cut(s) 272
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 365, 1079
BstDEI CTNAG 1 cut(s) 579
BstDSI CCRYGG 2 cut(s) 169, 818
BstEII GGTNACC 1 cut(s) 694
BstENI CCTNNNNNAGG 1 cut(s) 248
BstF5I GGATG 3 cut(s) 287, 849, 1121
BstFNI CGCG 1 cut(s) 720
BstHHI GCGC 1 cut(s) 720
BstKTI GATC 5 cut(s) 59, 527, 919, 1210, 1267
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 5 cut(s) 56, 524, 916, 1207, 1264
BstMWI GCNNNNNNNGC 7 cut(s) 142, 729, 941, 950, 1160, 1169, 1284
BstNI CCWGG 4 cut(s) 186, 245, 700, 1126
BstNSI RCATGY 2 cut(s) 1081, 1248
BstPI GGTNACC 1 cut(s) 694
BstSCI CCNGG 5 cut(s) 184, 243, 593, 698, 1124
BstSFI CTRYAG 1 cut(s) 1026
BstSLI GKGCMC 2 cut(s) 600, 1120
BstUI CGCG 1 cut(s) 720
BstV1I GCAGC 2 cut(s) 154, 962
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 2 cut(s) 524, 1264
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 2 cut(s) 524, 1264
BsuI GTATCC 1 cut(s) 789
BsuRI GGCC 6 cut(s) 184, 598, 629, 723, 817, 863
BtgI CCRYGG 2 cut(s) 169, 818
BtrI CACGTC 1 cut(s) 1157
BtsCI GGATG 3 cut(s) 287, 849, 1121
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 2 cut(s) 365, 1079
CaiI CAGNNNCTG 1 cut(s) 1175
CfoI GCGC 1 cut(s) 720
Cfr13I GGNCC 4 cut(s) 596, 597, 815, 861
Csp6I GTAC 4 cut(s) 92, 113, 661, 1037
CspCI CAANNNNNGTGG 2 cut(s) 589, 624
CviAII CATG 5 cut(s) 488, 665, 1078, 1184, 1245
CviQI GTAC 4 cut(s) 92, 113, 661, 1037
DdeI CTNAG 1 cut(s) 579
DpnI GATC 5 cut(s) 58, 526, 918, 1209, 1266
DpnII GATC 5 cut(s) 56, 524, 916, 1207, 1264
EaeI YGGCCR 3 cut(s) 182, 627, 721
Eam1104I CTCTTC 2 cut(s) 375, 1047
EarI CTCTTC 2 cut(s) 375, 1047
Eco24I GRGCYC 1 cut(s) 600
Eco72I CACGTG 1 cut(s) 272
Eco88I CYCGRG 2 cut(s) 858, 989
Eco91I GGTNACC 1 cut(s) 694
EcoNI CCTNNNNNAGG 1 cut(s) 248
EcoO65I GGTNACC 1 cut(s) 694
EcoRII CCWGG 4 cut(s) 184, 243, 698, 1124
EcoT22I ATGCAT 2 cut(s) 265, 481
EcoT38I GRGCYC 1 cut(s) 600
FaeI CATG 5 cut(s) 491, 668, 1081, 1187, 1248
FalI AAGNNNNNCTT 2 cut(s) 1156, 1188
FaqI GGGAC 2 cut(s) 560, 1176
FatI CATG 5 cut(s) 487, 664, 1077, 1183, 1244
FauI CCCGC 2 cut(s) 862, 1056
FauNDI CATATG 2 cut(s) 97, 109
Fnu4HI GCNGC 3 cut(s) 143, 721, 951
FokI GGATG 3 cut(s) 274, 836, 1108
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 3 cut(s) 143, 721, 951
FspBI CTAG 1 cut(s) 797
GlaI GCGC 1 cut(s) 719
GluI GCNGC 3 cut(s) 143, 721, 951
GsuI CTGGAG 1 cut(s) 266
HaeIII GGCC 6 cut(s) 184, 598, 629, 723, 817, 863
HapII CCGG 1 cut(s) 594
HhaI GCGC 1 cut(s) 720
Hin1II CATG 5 cut(s) 491, 668, 1081, 1187, 1248
Hin6I GCGC 1 cut(s) 718
HinP1I GCGC 1 cut(s) 718
HincII GTYRAC 1 cut(s) 74
HindII GTYRAC 1 cut(s) 74
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 3 cut(s) 70, 284, 454
HpaII CCGG 1 cut(s) 594
HphI GGTGA 2 cut(s) 685, 706
Hpy166II GTNNAC 5 cut(s) 74, 333, 1118, 1235, 1259
Hpy188I TCNGA 4 cut(s) 357, 1177, 1212, 1242
Hpy188III TCNNGA 4 cut(s) 33, 54, 86, 924
Hpy8I GTNNAC 5 cut(s) 74, 333, 1118, 1235, 1259
HpyAV CCTTC 3 cut(s) 826, 874, 1015
HpyCH4III ACNGT 4 cut(s) 173, 751, 1089, 1256
HpyCH4IV ACGT 4 cut(s) 22, 271, 1132, 1156
HpyF10VI GCNNNNNNNGC 7 cut(s) 142, 729, 941, 950, 1160, 1169, 1284
HpyF3I CTNAG 1 cut(s) 579
HpySE526I ACGT 4 cut(s) 22, 271, 1132, 1156
Hsp92II CATG 5 cut(s) 491, 668, 1081, 1187, 1248
HspAI GCGC 1 cut(s) 718
KpnI GGTACC 2 cut(s) 95, 1040
Kzo9I GATC 5 cut(s) 56, 524, 916, 1207, 1264
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 2 cut(s) 154, 962
LweI GCATC 4 cut(s) 250, 376, 466, 488
MaeI CTAG 1 cut(s) 797
MaeII ACGT 4 cut(s) 22, 271, 1132, 1156
MaeIII GTNAC 4 cut(s) 253, 694, 1089, 1250
MalI GATC 5 cut(s) 58, 526, 918, 1209, 1266
MbiI CCGCTC 1 cut(s) 857
MboI GATC 5 cut(s) 56, 524, 916, 1207, 1264
MboII GAAGA 7 cut(s) 392, 423, 455, 557, 1034, 1225, 1259
MfeI CAATTG 1 cut(s) 759
MflI RGATCY 2 cut(s) 524, 1264
MhlI GDGCHC 2 cut(s) 600, 1120
MlsI TGGCCA 2 cut(s) 184, 629
MluNI TGGCCA 2 cut(s) 184, 629
MlyI GAGTC 2 cut(s) 79, 293
MmeI TCCRAC 3 cut(s) 183, 781, 787
Mox20I TGGCCA 2 cut(s) 184, 629
Mph1103I ATGCAT 2 cut(s) 265, 481
MscI TGGCCA 2 cut(s) 184, 629
MseI TTAA 4 cut(s) 554, 728, 996, 1321
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 2 cut(s) 184, 629
MspI CCGG 1 cut(s) 594
MspR9I CCNGG 5 cut(s) 186, 245, 595, 700, 1126
MunI CAATTG 1 cut(s) 759
Mva1269I GAATGC 1 cut(s) 61
MvaI CCWGG 4 cut(s) 186, 245, 700, 1126
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 7 cut(s) 142, 729, 941, 950, 1160, 1169, 1284
NciI CCSGG 1 cut(s) 595
NdeI CATATG 2 cut(s) 97, 109
NdeII GATC 5 cut(s) 56, 524, 916, 1207, 1264
NlaIII CATG 5 cut(s) 491, 668, 1081, 1187, 1248
NlaIV GGNNCC 3 cut(s) 93, 598, 1038
NmuCI GTSAC 4 cut(s) 253, 694, 1089, 1250
NsiI ATGCAT 2 cut(s) 265, 481
NspI RCATGY 2 cut(s) 1081, 1248
NspV TTCGAA 1 cut(s) 17
OliI CACNNNNGTG 1 cut(s) 174
PaeI GCATGC 1 cut(s) 1081
PaeR7I CTCGAG 1 cut(s) 989
PciSI GCTCTTC 1 cut(s) 375
PctI GAATGC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 454
PflMI CCANNNNNTGG 1 cut(s) 670
PkrI GCNGC 3 cut(s) 144, 722, 952
PleI GAGTC 2 cut(s) 78, 292
PmaCI CACGTG 1 cut(s) 272
PmlI CACGTG 1 cut(s) 272
PpsI GAGTC 2 cut(s) 78, 292
Ppu21I YACGTR 1 cut(s) 272
Psp6I CCWGG 4 cut(s) 184, 243, 698, 1124
PspCI CACGTG 1 cut(s) 272
PspEI GGTNACC 1 cut(s) 694
PspGI CCWGG 4 cut(s) 184, 243, 698, 1124
PspN4I GGNNCC 3 cut(s) 93, 598, 1038
PspOMI GGGCCC 1 cut(s) 596
PspPI GGNCC 4 cut(s) 596, 597, 815, 861
PspXI VCTCGAGB 1 cut(s) 989
PstNI CAGNNNCTG 1 cut(s) 1175
PsuI RGATCY 2 cut(s) 524, 1264
RsaI GTAC 4 cut(s) 93, 114, 662, 1038
RsaNI GTAC 4 cut(s) 92, 113, 661, 1037
RseI CAYNNNNRTG 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 4 cut(s) 554, 728, 996, 1321
SatI GCNGC 3 cut(s) 143, 721, 951
Sau3AI GATC 5 cut(s) 56, 524, 916, 1207, 1264
Sau96I GGNCC 4 cut(s) 596, 597, 815, 861
SchI GAGTC 2 cut(s) 79, 293
ScrFI CCNGG 5 cut(s) 186, 245, 595, 700, 1126
SduI GDGCHC 2 cut(s) 600, 1120
SfaNI GCATC 4 cut(s) 250, 376, 466, 488
SfcI CTRYAG 1 cut(s) 1026
Sfr274I CTCGAG 1 cut(s) 989
SfuI TTCGAA 1 cut(s) 17
SlaI CTCGAG 1 cut(s) 989
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
SpeI ACTAGT 1 cut(s) 796
SphI GCATGC 1 cut(s) 1081
SsiI CCGC 3 cut(s) 720, 855, 1049
SspMI CTAG 1 cut(s) 797
StyD4I CCNGG 5 cut(s) 184, 243, 593, 698, 1124
TaaI ACNGT 4 cut(s) 173, 751, 1089, 1256
TaiI ACGT 4 cut(s) 25, 274, 1135, 1159
TaqI TCGA 6 cut(s) 17, 32, 191, 867, 923, 990
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 1 cut(s) 454
Tru1I TTAA 4 cut(s) 554, 728, 996, 1321
Tru9I TTAA 4 cut(s) 554, 728, 996, 1321
TseFI GTSAC 4 cut(s) 253, 694, 1089, 1250
TseI GCWGC 2 cut(s) 142, 950
Tsp45I GTSAC 4 cut(s) 253, 694, 1089, 1250
TspDTI ATGAA 4 cut(s) 341, 411, 456, 845
Van91I CCANNNNNTGG 1 cut(s) 670
VneI GTGCAC 1 cut(s) 1116
XagI CCTNNNNNAGG 1 cut(s) 248
XapI RAATTY 3 cut(s) 836, 886, 938
XceI RCATGY 2 cut(s) 1081, 1248
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 989
XspI CTAG 1 cut(s) 797
Zsp2I ATGCAT 2 cut(s) 265, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.