Rorug06G0513200

E3 ubiquitin-protein ligase RGLG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000761
Physical Location & Seq
Reverse (-)
62790361 .. 62790744
384 bp
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UTR
Exon/CDS
Intron
Rorug06G0513200.1

Sequence Viewer

Length: 384 bp
ATGGGTTCAGATTCTCATTACCAGATTCCAGCCATAGCATTCACAATCAACTCGCGGGAGGTGGACCGAGGAACTGATGAATGGTACCATCTGTGCAATATGGTTCGAGAGGCTTGTGAAAATTATGGTTGCTTTGAAATAGTGTATGATAAGATACCTCCGCAGTTAAGAGCTGAAACCTTCTCTGTGTCAAGGCAACTTTTCAGCCTTCCACTTGAGAAAAAGAAGAAGAACCTTAATCCAAAGCCTTACCAGGGTTACCTTGCACCAAGTGTCCGGGCTCCTTTGTATGAGAGCTTTGGCCTAGAGAAAGCCTCCAACTATGAATCCCTTAGAAGCTTGACAGAAGAAATATCGCCTAATGGTCATGACCAGTTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Copine zf-C3HC4_3

Protein Analysis

127

Amino Acids

14.62

Weight (kDa)

5.6

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 25 - 90 8.5e-15 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 84
AccII CGCG 1 cut(s) 55
AciI CCGC 2 cut(s) 55, 161
AdeI CACNNNGTG 1 cut(s) 272
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 1 cut(s) 137
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 3 cut(s) 173, 297, 339
AluI AGCT 3 cut(s) 173, 297, 339
AoxI GGCC 1 cut(s) 301
Asp718I GGTACC 1 cut(s) 84
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 278
AvaII GGWCC 1 cut(s) 64
BanI GGYRCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 283
BccI CCATC 1 cut(s) 96
BciT130I CCWGG 1 cut(s) 254
BcnI CCSGG 1 cut(s) 278
BfaI CTAG 1 cut(s) 305
Bme1390I CCNGG 2 cut(s) 254, 278
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 2 cut(s) 86, 282
BmrFI CCNGG 2 cut(s) 254, 278
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 278
BsaJI CCNNGG 2 cut(s) 67, 253
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 373
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 67, 253
BseLI CCNNNNNNNGG 1 cut(s) 254
BseNI ACTGG 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 84
BsiSI CCGG 1 cut(s) 277
BslI CCNNNNNNNGG 1 cut(s) 254
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 283
BspACI CCGC 2 cut(s) 55, 161
BspANI GGCC 1 cut(s) 303
BspFNI CGCG 1 cut(s) 55
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 86, 282
BspT107I GGYRCC 1 cut(s) 84
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 2 cut(s) 67, 253
Bst2UI CCWGG 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 332
BstEII GGTNACC 1 cut(s) 257
BstFNI CGCG 1 cut(s) 55
BstNI CCWGG 1 cut(s) 254
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 2 cut(s) 252, 276
BstUI CGCG 1 cut(s) 55
BsuRI GGCC 1 cut(s) 303
CciI TCATGA 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 64
Csp6I GTAC 1 cut(s) 85
CviAII CATG 1 cut(s) 368
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 332
DraIII CACNNNGTG 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 64
Eco91I GGTNACC 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 283
FaeI CATG 1 cut(s) 371
FaiI YATR 7 cut(s) 35, 101, 126, 147, 291, 324, 369
FatI CATG 1 cut(s) 367
FauI CCCGC 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 283
FspBI CTAG 1 cut(s) 305
HaeIII GGCC 1 cut(s) 303
HapII CCGG 1 cut(s) 277
Hin1II CATG 1 cut(s) 371
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 3 cut(s) 11, 25, 326
HpaII CCGG 1 cut(s) 277
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 10
Hpy188III TCNNGA 2 cut(s) 107, 368
Hpy8I GTNNAC 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 190, 218
HpyCH4V TGCA 2 cut(s) 96, 266
HpyF3I CTNAG 1 cut(s) 332
Hsp92II CATG 1 cut(s) 371
KpnI GGTACC 1 cut(s) 88
LmnI GCTCC 1 cut(s) 286
LpnPI CCDG 5 cut(s) 35, 42, 239, 266, 290
MaeI CTAG 1 cut(s) 305
MaeIII GTNAC 1 cut(s) 257
MboII GAAGA 3 cut(s) 238, 241, 359
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 1 cut(s) 121
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 5 cut(s) 52, 62, 103, 168, 325
MseI TTAA 3 cut(s) 167, 237, 382
MspI CCGG 1 cut(s) 277
MspR9I CCNGG 2 cut(s) 254, 278
Mva1269I GAATGC 1 cut(s) 38
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 1 cut(s) 55
NciI CCSGG 1 cut(s) 278
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 86, 282
PagI TCATGA 1 cut(s) 367
PctI GAATGC 1 cut(s) 38
PfeI GAWTC 3 cut(s) 11, 25, 326
Psp6I CCWGG 1 cut(s) 252
PspEI GGTNACC 1 cut(s) 257
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 2 cut(s) 86, 282
PspPI GGNCC 1 cut(s) 64
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SaqAI TTAA 3 cut(s) 167, 237, 382
Sau96I GGNCC 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 254, 278
SduI GDGCHC 1 cut(s) 283
SetI ASST 8 cut(s) 63, 160, 175, 182, 237, 264, 299, 341
SinI GGWCC 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 1 cut(s) 121
SsiI CCGC 2 cut(s) 55, 161
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 2 cut(s) 252, 276
TaqI TCGA 1 cut(s) 106
TaqII GACCGA 1 cut(s) 81
TasI AATT 1 cut(s) 121
TfiI GAWTC 3 cut(s) 11, 25, 326
Tru1I TTAA 3 cut(s) 167, 237, 382
Tru9I TTAA 3 cut(s) 167, 237, 382
TspDTI ATGAA 2 cut(s) 93, 339
VpaK11BI GGWCC 1 cut(s) 64
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.