RLG00000004403

negative regulation of response to water deprivation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000761
Physical Location & Seq
Forward (+)
59081334 .. 59086590
5257 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004403

Sequence Viewer

Length: 2913 bp
ATGGGTGGGATAACTTCAAAAGCTAGTACATCGAGACAGTCTTCTTTTGCGTCTAGATCATCTTCTTGGAATCATCAGAATTATGAACAGGCGCCGTATGCACAACCATATGCACCTCCAAGCCATGACGCTGGGCAACAGCAGCATTATGCACCTCCAACTCAAAGCTATGGTGGTGGTCCTTGGGCATCAGAGTCAAAAAAGAAGTTGGAAAGAAAGTATTCGAAGATAGATGATAATTACAACAGCCTGGACCAGGTCACAGATGCATTGGCACGTGCTGGTCTAGAGTCATCAAATCTTATTGTTGGTATTGATTTCACAAAGAGCAATGAGTGGACAGGTGCTAGGTCATTTCACCGGAAAAGCTTGCATCACATTGGAGAAGAGCAAAATCCCTATGAACAAGCAATAGCAATTATTGGAAGAACATTATCTTCTTTCGATGAGGATAACATGATTCCTTGTTATGGATTTGGAGATGCATCAACCCACGACCAAGAAGTTTTCAGTTTCTACCCAGATGAGGACACTTATTGCAATGGATTTGAAGAAGTTTTAAGACGATATAGAGAATTAGTCCCTCAGCTACGACTTGCTGGGCCCACATCATTTGCCCCTATCGTTGAAATGGCCATCACTATAGTTGAACAAAGTGGTGGTCAGTATCATGTGTTAGTGATAATAGCTGATGGGCAGGTGACTAGAAGTGTTGAGACCGGTCGTGGCCAGTTAAGCCCACAGGAAAAGAAAACTGTTGACGCAATTGTCAAAGCAAGTGAGTACCCATTGTCAATTATAGTGGTTGGAGTTGGAGATGGACCATGGGACATGATGAAAGAATTTGATGATAACATTCCTGCACGAGCCTTTGATAACTTTCAGTTTGTGAATTTTACCGACATTATGTCAAAGAATACAGACCGCTCAAGAAAAGAAGCAGAATTTGCTCTTGCAGCCTTGATGGAAATACCCTCTCAGTATAAAGCGACGCTAGAGCTTGGTATATTGGGTGTTACAAGAGGGAAGGCTGTAGATAGGATCCCGCTTCCCCCTCCTCATTACGGTTCGGCTTCTTTTGGCACCTCAAAACCTTCACGTGCAAATAATTTTCGTCCAACTGCACCTTCCAGGCCACAACATCAGGGTGTTAGCAATAGCAGAGTGCCTCCTGCTGCTTCTGCTTCTGACAATCATGTCTGTCCCATTTGCCTAACCGATCCAAAGAATATGGCATTTGGTTGTGGACATCAGACATGCTGCGACTGCGGACAAGATCTTCAGGGGTCCTCGAACGGAGATGGGAAAGGGCAGTGGCAACTGCTTCTGGACAATACTGAAGTGGTGGCAATGCATATGACGTTAACTCACCACAACACGGTCATTATCTTTGATCAAACAGAAGCAGGTCCTTCTGGGCACCGGCTTAGAAGGCGCTACAATGGAACTAGGTGTACTCAATTCCGCAATGACTTGGCAGACTCATCATGCTATGTTCACTCGGTTGAGTATGACATTTCTGGTAACAAGCTGAGACCTCTGAGGCTTTCCACTGATACATGGTGCTCCTCAGGTTCCTTCTTGAGCAATGGGACACTTCTACAAACCGGTGGACATGGAAGCGGTGCTCAAGGAATTCGATACTTTAGTCCTTGTGGGGAAAATGATAACTGCGACTGGAGGGAATCGCGGCAATCACTAGCTGATGATCGTTGGTATGCTTCCAGTCTACTACTCCCAGACGAGGATCGGGTTATTGTGGTAGGTGGAAGAAGGGTCTTTACTTATGAATTTATACCAAAGCAGTATTCCGATGAAGGCTCTTTTGACTTTCCATTCTTGCGCAACACACATCAAAGAAGTGAAGGAGGGAACAACCTCTATCCCTTCCTTCACATTTGTCCAGATGGCAGATTGTTCATTTTTGCCAACCGAGACTCGATTCTCTTCAACTACAAAGAGAACAGGGTGGTCAAGCACTTCCCACGCATACCCGGAGATGACTCCAGGAACTACCCGAGCTCTGGCTCGTCAGTCATCCTCCCTTTACGTTACACAAACGGCTTTGAAAAGGCGGAAGTCATGGTGTGTGGTGGTGCGGCCAGTGGAGCTTACAGTGCAGCAGGAGATGGCAGGTTCTTGAAAGGTTTGAGCTCTTGTGGAAGAATGGTGATCACAGGTAACAGACACAAGTGGAACATGGAGAACATGCCCGGTCCACGCCTCCTATGCGACATGCTGCTCCTCCCAACCGGAGATGTCCTGATCATCAACGGGGCTAAACGTGGTGCTGGAGGTTGGAACAATGCAAGAAATGCTTCACTCGAGCCTTACCTTTACCACCCAAACAAGTCAGTAGGCAAAAGGTTCTCAGTGCTCAAGTCAACACGAATTCCTAGAATGTATCACTCCTCAGCCATTCTCTTACCTGATGGGAGGGTTTTAGTTGCCGGTGGTAACCCTCACAATAGGTACGTTTTCGAAAATGTGGCATACCCAACTGAGCTTAGGCTACAAGCATTTGTTCCAGACTATATGGGTAGTCAACACCATCAATTTAGACCCACTAATGTGACCATACACTATGGCCGTAATGATCTTTATGGTGTGAGGTATGGAGGAGAGTTCAATGTGAGCTTTTGGTTGGCGAGTAAGCCGAGCAATGCGGTGGAGTTCAGTGCTTATGCTCCGCCATTCACGTCACACTCGATATCGATGAATCAGAGGATGCTGAGGCTGAGGTGCAAGAGTTTGGTGAGGGGTCAAGATGGATGGGTAGTTGCAGTGTTGGAGGCTCCTCCCTCTCCTTCGGTTGCGCCAGCGGGTTATTACTTGCTTACTGTTTTGAACGGTGGAATTCCTAGTCTGTCTCAGTGGATTAGGATCCTACAGATTTCTGGAGGTCGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0001786 GO:0003674 GO:0005215 GO:0005488 GO:0005509 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0007154 GO:0007267 GO:0007268 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008289 GO:0008610 GO:0008654 GO:0009058 GO:0009987 GO:0010035 GO:0010038 GO:0010720 GO:0010975 GO:0010976 GO:0016020 GO:0016192 GO:0019637 GO:0022008 GO:0022603 GO:0022604 GO:0023052 GO:0030135 GO:0030136 GO:0030139 GO:0030154 GO:0030307 GO:0030424 GO:0030425 GO:0031344 GO:0031346 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0036477 GO:0040008 GO:0042221 GO:0042995 GO:0043005 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0045017 GO:0045334 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0046474 GO:0046486 GO:0046872 GO:0048518 GO:0048522 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051592 GO:0051716 GO:0051960 GO:0051962 GO:0060284 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071277 GO:0071704 GO:0071944 GO:0072341 GO:0090407 GO:0097447 GO:0097458 GO:0097708 GO:0098916 GO:0099536 GO:0099537 GO:0120025 GO:0120035 GO:0120038 GO:1901576 GO:1903859 GO:1903861 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine zf-C3HC4_3

Protein Analysis

971

Amino Acids

107.45

Weight (kDa)

8.0

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Copine PF07002 123 - 336 2.1e-73 Copine
Glyoxal_oxid_N PF07250 451 - 845 7.7e-169 Glyoxal oxidase N-terminus
GO-like_E_set PF09118 870 - 962 3.1e-21 Galactose oxidase-like, Early set domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 688
AasI GACNNNNNNGTC 2 cut(s) 767, 1196
Acc16I TGCGCA 1 cut(s) 1844
Acc36I ACCTGC 3 cut(s) 688, 1397, 2124
AccB1I GGYRCC 3 cut(s) 91, 1082, 1419
AccBSI CCGCTC 1 cut(s) 927
AccI GTMKAC 1 cut(s) 1729
AccII CGCG 1 cut(s) 1690
AclWI GGATC 6 cut(s) 1036, 1049, 1214, 1755, 2878, 2891
AcoI YGGCCR 4 cut(s) 633, 727, 2100, 2587
AcsI RAATTY 7 cut(s) 842, 892, 944, 1635, 1790, 2391, 2856
AcuI CTGAAG 2 cut(s) 1265, 1359
AcvI CACGTG 2 cut(s) 278, 1100
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 4 cut(s) 28, 785, 1456, 2474
AfiI CCNNNNNNNGG 7 cut(s) 256, 470, 526, 1064, 1378, 1744, 2024
AgeI ACCGGT 2 cut(s) 719, 1607
AgsI TTSAA 9 cut(s) 18, 551, 629, 650, 1951, 2069, 2143, 2629, 2848
AhdI GACNNNNNGTC 1 cut(s) 907
AjiI CACGTC 1 cut(s) 2700
AjnI CCWGG 4 cut(s) 249, 255, 1130, 2006
AjuI GAANNNNNNNTTGG 4 cut(s) 1111, 1143, 1792, 1824
AleI CACNNNNGTG 1 cut(s) 2570
Alw21I GWGCWC 5 cut(s) 1568, 1630, 2024, 2156, 2379
Alw26I GTCTC 5 cut(s) 28, 710, 1528, 1929, 2874
AlwI GGATC 6 cut(s) 1036, 1049, 1214, 1755, 2878, 2891
Ama87I CYCGRG 2 cut(s) 2017, 2324
AoxI GGCC 6 cut(s) 602, 633, 727, 1133, 2100, 2587
ApaI GGGCCC 1 cut(s) 606
ApeKI GCWGC 6 cut(s) 142, 956, 1175, 1260, 2120, 2239
ApoI RAATTY 7 cut(s) 842, 892, 944, 1635, 1790, 2391, 2856
ArsI GACNNNNNNTTYG 2 cut(s) 646, 678
AsiGI ACCGGT 2 cut(s) 719, 1607
Asp700I GAANNNNTTC 2 cut(s) 220, 2317
AspLEI GCGC 4 cut(s) 94, 1437, 1845, 2818
AspS9I GGNCC 8 cut(s) 179, 253, 602, 603, 821, 1287, 1409, 2216
AsuC2I CCSGG 2 cut(s) 1995, 2214
AsuHPI GGTGA 5 cut(s) 350, 712, 1361, 2182, 2767
AsuII TTCGAA 2 cut(s) 224, 2481
AvaI CYCGRG 2 cut(s) 2017, 2324
AvaII GGWCC 6 cut(s) 179, 253, 821, 1287, 1409, 2216
AxyI CCTNAGG 1 cut(s) 1570
BaeGI GKGCMC 2 cut(s) 606, 1422
BaeI ACNNNNGTAYC 2 cut(s) 1633, 1666
BalI TGGCCA 2 cut(s) 635, 729
BamHI GGATCC 2 cut(s) 1041, 2883
BanI GGYRCC 3 cut(s) 91, 1082, 1419
BanII GRGCYC 3 cut(s) 606, 2024, 2156
BarI GAAGNNNNNNTAC 2 cut(s) 1765, 1797
BauI CACGAG 1 cut(s) 864
BbrPI CACGTG 2 cut(s) 278, 1100
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 5 cut(s) 1568, 1630, 2024, 2156, 2379
BbvCI CCTCAGC 4 cut(s) 585, 2413, 2732, 2738
BbvI GCAGC 6 cut(s) 154, 968, 1162, 1247, 2132, 2226
BceAI ACGGC 3 cut(s) 79, 2077, 2574
BciT130I CCWGG 4 cut(s) 251, 257, 1132, 2008
BclI TGATCA 3 cut(s) 1393, 2172, 2265
BcnI CCSGG 2 cut(s) 1995, 2214
BcoDI GTCTC 5 cut(s) 28, 710, 1528, 1929, 2874
BfmI CTRYAG 3 cut(s) 642, 1032, 2888
BfoI RGCGCY 2 cut(s) 95, 1438
BfuAI ACCTGC 3 cut(s) 688, 1397, 2124
BglII AGATCT 1 cut(s) 1276
BisI GCNGC 8 cut(s) 143, 957, 1176, 1261, 1691, 2100, 2121, 2240
BlsI GCNGC 8 cut(s) 144, 958, 1177, 1262, 1692, 2101, 2122, 2241
Bme1390I CCNGG 6 cut(s) 251, 257, 1132, 1995, 2008, 2214
Bme18I GGWCC 6 cut(s) 179, 253, 821, 1287, 1409, 2216
BmeRI GACNNNNNGTC 1 cut(s) 907
BmeT110I CYCGRG 2 cut(s) 2017, 2324
BmgBI CACGTC 1 cut(s) 2700
BmgT120I GGNCC 8 cut(s) 179, 253, 602, 603, 821, 1287, 1409, 2216
BmiI GGNNCC 9 cut(s) 93, 604, 1043, 1084, 1288, 1421, 1576, 2796, 2885
BmrFI CCNGG 6 cut(s) 251, 257, 1132, 1995, 2008, 2214
BmsI GCATC 6 cut(s) 197, 256, 382, 472, 494, 2718
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 3 cut(s) 1699, 1990, 2313
Bpu10I CCTNAGC 5 cut(s) 585, 2413, 2507, 2732, 2738
Bpu14I TTCGAA 2 cut(s) 224, 2481
BpuEI CTTGAG 4 cut(s) 913, 1603, 1614, 2363
BpuMI CCSGG 2 cut(s) 1995, 2214
Bsa29I ATCGAT 1 cut(s) 2714
BsaAI YACGTR 2 cut(s) 278, 1100
BsaBI GATNNNNATC 1 cut(s) 2882
BsaHI GRCGYC 1 cut(s) 92
BsaI GGTCTC 2 cut(s) 710, 1528
BsaJI CCNNGG 2 cut(s) 182, 824
BsaWI WCCGGW 4 cut(s) 360, 719, 1607, 2252
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 470, 526, 1064, 1378, 1744, 2024
Bse118I RCCGGY 4 cut(s) 719, 1422, 1607, 2450
Bse1I ACTGG 4 cut(s) 730, 1682, 1725, 2103
Bse21I CCTNAGG 1 cut(s) 1570
Bse3DI GCAATG 6 cut(s) 337, 547, 1356, 1474, 1594, 2668
Bse8I GATNNNNATC 1 cut(s) 2882
BseBI CCWGG 4 cut(s) 251, 257, 1132, 2008
BseCI ATCGAT 1 cut(s) 2714
BseDI CCNNGG 2 cut(s) 182, 824
BseGI GGATG 3 cut(s) 2037, 2733, 2777
BseJI GATNNNNATC 1 cut(s) 2882
BseLI CCNNNNNNNGG 7 cut(s) 256, 470, 526, 1064, 1378, 1744, 2024
BseMI GCAATG 6 cut(s) 337, 547, 1356, 1474, 1594, 2668
BseNI ACTGG 4 cut(s) 730, 1682, 1725, 2103
BseRI GAGGAG 6 cut(s) 1047, 1558, 2234, 2401, 2634, 2787
BseSI GKGCMC 2 cut(s) 606, 1422
BseXI GCAGC 6 cut(s) 154, 968, 1162, 1247, 2132, 2226
BseYI CCCAGC 2 cut(s) 131, 599
BsgI GTGCAG 3 cut(s) 846, 1107, 2139
Bsh1236I CGCG 1 cut(s) 1690
Bsh1285I CGRYCG 1 cut(s) 724
BshFI GGCC 6 cut(s) 604, 635, 729, 1135, 2102, 2589
BshNI GGYRCC 3 cut(s) 91, 1082, 1419
BshTI ACCGGT 2 cut(s) 719, 1607
BshVI ATCGAT 1 cut(s) 2714
BsiEI CGRYCG 1 cut(s) 724
BsiHKAI GWGCWC 5 cut(s) 1568, 1630, 2024, 2156, 2379
BsiHKCI CYCGRG 2 cut(s) 2017, 2324
BsiSI CCGG 8 cut(s) 361, 720, 1423, 1608, 1995, 2214, 2253, 2451
BslFI GGGAC 4 cut(s) 566, 842, 1188, 1606
BslI CCNNNNNNNGG 7 cut(s) 256, 470, 526, 1064, 1378, 1744, 2024
BsmAI GTCTC 5 cut(s) 28, 710, 1528, 1929, 2874
BsmFI GGGAC 4 cut(s) 566, 842, 1188, 1606
BsnI GGCC 6 cut(s) 604, 635, 729, 1135, 2102, 2589
Bso31I GGTCTC 2 cut(s) 710, 1528
BsoBI CYCGRG 2 cut(s) 2017, 2324
Bsp119I TTCGAA 2 cut(s) 224, 2481
Bsp120I GGGCCC 1 cut(s) 602
Bsp1286I GDGCHC 7 cut(s) 606, 1422, 1568, 1630, 2024, 2156, 2379
Bsp19I CCATGG 1 cut(s) 824
BspANI GGCC 6 cut(s) 604, 635, 729, 1135, 2102, 2589
BspDI ATCGAT 1 cut(s) 2714
BspFNI CGCG 1 cut(s) 1690
BspLI GGNNCC 9 cut(s) 93, 604, 1043, 1084, 1288, 1421, 1576, 2796, 2885
BspMI ACCTGC 3 cut(s) 688, 1397, 2124
BspPI GGATC 6 cut(s) 1036, 1049, 1214, 1755, 2878, 2891
BspQI GCTCTTC 1 cut(s) 381
BspT104I TTCGAA 2 cut(s) 224, 2481
BspT107I GGYRCC 3 cut(s) 91, 1082, 1419
BspTNI GGTCTC 2 cut(s) 710, 1528
BsrBI CCGCTC 1 cut(s) 927
BsrDI GCAATG 6 cut(s) 337, 547, 1356, 1474, 1594, 2668
BsrFI RCCGGY 4 cut(s) 719, 1422, 1607, 2450
BsrI ACTGG 4 cut(s) 730, 1682, 1725, 2103
BssAI RCCGGY 4 cut(s) 719, 1422, 1607, 2450
BssECI CCNNGG 2 cut(s) 182, 824
BssNI GRCGYC 1 cut(s) 92
BssSI CACGAG 1 cut(s) 864
BssT1I CCWWGG 2 cut(s) 182, 824
Bst2BI CACGAG 1 cut(s) 864
Bst2UI CCWGG 4 cut(s) 251, 257, 1132, 2008
Bst4CI ACNGT 7 cut(s) 39, 757, 1067, 1381, 2117, 2842, 2852
Bst6I CTCTTC 2 cut(s) 381, 1952
BstACI GRCGYC 1 cut(s) 92
BstAPI GCANNNNNTGC 2 cut(s) 947, 2315
BstBAI YACGTR 2 cut(s) 278, 1100
BstBI TTCGAA 2 cut(s) 224, 2481
BstC8I GCNNGC 2 cut(s) 371, 2820
BstDSI CCRYGG 1 cut(s) 824
BstEII GGTNACC 1 cut(s) 2456
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 3 cut(s) 2037, 2733, 2777
BstFNI CGCG 1 cut(s) 1690
BstH2I RGCGCY 2 cut(s) 95, 1438
BstHHI GCGC 4 cut(s) 94, 1437, 1845, 2818
BstMAI GTCTC 5 cut(s) 28, 710, 1528, 1929, 2874
BstMCI CGRYCG 1 cut(s) 724
BstNI CCWGG 4 cut(s) 251, 257, 1132, 2008
BstNSI RCATGY 3 cut(s) 1260, 2212, 2239
BstPI GGTNACC 1 cut(s) 2456
BstSCI CCNGG 6 cut(s) 249, 255, 1130, 1993, 2006, 2212
BstSFI CTRYAG 3 cut(s) 642, 1032, 2888
BstSLI GKGCMC 2 cut(s) 606, 1422
BstUI CGCG 1 cut(s) 1690
BstV1I GCAGC 6 cut(s) 154, 968, 1162, 1247, 2132, 2226
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 3 cut(s) 1041, 1276, 2883
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 3 cut(s) 1041, 1276, 2883
Bsu15I ATCGAT 1 cut(s) 2714
Bsu36I CCTNAGG 1 cut(s) 1570
BsuRI GGCC 6 cut(s) 604, 635, 729, 1135, 2102, 2589
BsuTUI ATCGAT 1 cut(s) 2714
BtgI CCRYGG 1 cut(s) 824
BtrI CACGTC 1 cut(s) 2700
BtsCI GGATG 3 cut(s) 2037, 2733, 2777
BtsI GCAGTG 2 cut(s) 1319, 2790
BtsIMutI CAGTG 8 cut(s) 1319, 1551, 2110, 2122, 2379, 2683, 2790, 2879
BveI ACCTGC 3 cut(s) 688, 1397, 2124
Cac8I GCNNGC 2 cut(s) 371, 2820
CfoI GCGC 4 cut(s) 94, 1437, 1845, 2818
Cfr10I RCCGGY 4 cut(s) 719, 1422, 1607, 2450
Cfr13I GGNCC 8 cut(s) 179, 253, 602, 603, 821, 1287, 1409, 2216
ClaI ATCGAT 1 cut(s) 2714
CseI GACGC 4 cut(s) 39, 137, 770, 1000
CsiI ACCWGGT 1 cut(s) 255
Csp6I GTAC 4 cut(s) 27, 784, 1455, 2473
CspAI ACCGGT 2 cut(s) 719, 1607
CspCI CAANNNNNGTGG 4 cut(s) 595, 630, 783, 818
CviQI GTAC 4 cut(s) 27, 784, 1455, 2473
DinI GGCGCC 1 cut(s) 93
DrdI GACNNNNNNGTC 2 cut(s) 767, 1196
DriI GACNNNNNGTC 1 cut(s) 907
DseDI GACNNNNNNGTC 2 cut(s) 767, 1196
EaeI YGGCCR 4 cut(s) 633, 727, 2100, 2587
Eam1104I CTCTTC 2 cut(s) 381, 1952
Eam1105I GACNNNNNGTC 1 cut(s) 907
EarI CTCTTC 2 cut(s) 381, 1952
EciI GGCGGA 2 cut(s) 2090, 2679
Ecl136II GAGCTC 2 cut(s) 2022, 2154
Eco130I CCWWGG 2 cut(s) 182, 824
Eco24I GRGCYC 3 cut(s) 606, 2024, 2156
Eco31I GGTCTC 2 cut(s) 710, 1528
Eco32I GATATC 1 cut(s) 2712
Eco47I GGWCC 6 cut(s) 179, 253, 821, 1287, 1409, 2216
Eco53kI GAGCTC 2 cut(s) 2022, 2154
Eco57I CTGAAG 2 cut(s) 1265, 1359
Eco72I CACGTG 2 cut(s) 278, 1100
Eco81I CCTNAGG 1 cut(s) 1570
Eco88I CYCGRG 2 cut(s) 2017, 2324
Eco91I GGTNACC 1 cut(s) 2456
EcoICRI GAGCTC 2 cut(s) 2022, 2154
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoO109I RGGNCCY 2 cut(s) 1287, 1409
EcoO65I GGTNACC 1 cut(s) 2456
EcoRI GAATTC 3 cut(s) 1635, 2391, 2856
EcoRII CCWGG 4 cut(s) 249, 255, 1130, 2006
EcoRV GATATC 1 cut(s) 2712
EcoT14I CCWWGG 2 cut(s) 182, 824
EcoT22I ATGCAT 3 cut(s) 271, 487, 1356
EcoT38I GRGCYC 3 cut(s) 606, 2024, 2156
EgeI GGCGCC 1 cut(s) 93
EheI GGCGCC 1 cut(s) 93
ErhI CCWWGG 2 cut(s) 182, 824
FalI AAGNNNNNCTT 2 cut(s) 2302, 2334
FaqI GGGAC 4 cut(s) 566, 842, 1188, 1606
FauI CCCGC 2 cut(s) 1053, 2815
FauNDI CATATG 2 cut(s) 109, 1356
FbaI TGATCA 3 cut(s) 1393, 2172, 2265
FblI GTMKAC 1 cut(s) 1729
Fnu4HI GCNGC 8 cut(s) 143, 957, 1176, 1261, 1691, 2100, 2121, 2240
FokI GGATG 3 cut(s) 2024, 2740, 2784
FriOI GRGCYC 3 cut(s) 606, 2024, 2156
Fsp4HI GCNGC 8 cut(s) 143, 957, 1176, 1261, 1691, 2100, 2121, 2240
FspI TGCGCA 1 cut(s) 1844
GlaI GCGC 4 cut(s) 93, 1436, 1844, 2817
GluI GCNGC 8 cut(s) 143, 957, 1176, 1261, 1691, 2100, 2121, 2240
GsaI CCCAGC 2 cut(s) 135, 603
GsuI CTGGAG 3 cut(s) 1699, 1990, 2313
HaeII RGCGCY 2 cut(s) 95, 1438
HaeIII GGCC 6 cut(s) 604, 635, 729, 1135, 2102, 2589
HapII CCGG 8 cut(s) 361, 720, 1423, 1608, 1995, 2214, 2253, 2451
HgaI GACGC 4 cut(s) 39, 137, 770, 1000
HhaI GCGC 4 cut(s) 94, 1437, 1845, 2818
Hin1I GRCGYC 1 cut(s) 92
Hin6I GCGC 4 cut(s) 92, 1435, 1843, 2816
HinP1I GCGC 4 cut(s) 92, 1435, 1843, 2816
HincII GTYRAC 4 cut(s) 760, 1365, 2385, 2546
HindII GTYRAC 4 cut(s) 760, 1365, 2385, 2546
HindIII AAGCTT 1 cut(s) 367
HpaI GTTAAC 1 cut(s) 1365
HpaII CCGG 8 cut(s) 361, 720, 1423, 1608, 1995, 2214, 2253, 2451
HphI GGTGA 5 cut(s) 350, 712, 1361, 2182, 2767
Hpy188I TCNGA 7 cut(s) 78, 193, 1189, 1254, 1542, 1813, 2724
Hpy99I CGWCG 1 cut(s) 994
HpyCH4III ACNGT 7 cut(s) 39, 757, 1067, 1381, 2117, 2842, 2852
HpyCH4IV ACGT 7 cut(s) 277, 1099, 1361, 2050, 2284, 2475, 2699
HpySE526I ACGT 7 cut(s) 277, 1099, 1361, 2050, 2284, 2475, 2699
Hsp92I GRCGYC 1 cut(s) 92
HspAI GCGC 4 cut(s) 92, 1435, 1843, 2816
KasI GGCGCC 1 cut(s) 91
Ksp22I TGATCA 3 cut(s) 1393, 2172, 2265
KspAI GTTAAC 1 cut(s) 1365
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 5 cut(s) 1571, 2108, 2247, 2692, 2800
Lsp1109I GCAGC 6 cut(s) 154, 968, 1162, 1247, 2132, 2226
LweI GCATC 6 cut(s) 197, 256, 382, 472, 494, 2718
MabI ACCWGGT 1 cut(s) 255
MaeII ACGT 7 cut(s) 277, 1099, 1361, 2050, 2284, 2475, 2699
MaeIII GTNAC 9 cut(s) 259, 700, 1015, 1523, 2051, 2180, 2456, 2572, 2700
MbiI CCGCTC 1 cut(s) 927
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 3 cut(s) 1041, 1276, 2883
MhlI GDGCHC 7 cut(s) 606, 1422, 1568, 1630, 2024, 2156, 2379
MlsI TGGCCA 2 cut(s) 635, 729
MluNI TGGCCA 2 cut(s) 635, 729
Mly113I GGCGCC 1 cut(s) 92
MlyI GAGTC 5 cut(s) 203, 299, 1475, 1931, 1997
MmeI TCCRAC 7 cut(s) 182, 189, 787, 793, 1142, 2279, 2769
Mox20I TGGCCA 2 cut(s) 635, 729
Mph1103I ATGCAT 3 cut(s) 271, 487, 1356
MroXI GAANNNNTTC 2 cut(s) 220, 2317
MscI TGGCCA 2 cut(s) 635, 729
MseI TTAA 3 cut(s) 560, 734, 1364
MslI CAYNNNNRTG 2 cut(s) 1146, 2570
Msp20I TGGCCA 2 cut(s) 635, 729
MspA1I CMGCKG 1 cut(s) 2822
MspI CCGG 8 cut(s) 361, 720, 1423, 1608, 1995, 2214, 2253, 2451
MspR9I CCNGG 6 cut(s) 251, 257, 1132, 1995, 2008, 2214
MunI CAATTG 1 cut(s) 765
MvaI CCWGG 4 cut(s) 251, 257, 1132, 2008
MvnI CGCG 1 cut(s) 1690
NarI GGCGCC 1 cut(s) 92
NciI CCSGG 2 cut(s) 1995, 2214
NcoI CCATGG 1 cut(s) 824
NdeI CATATG 2 cut(s) 109, 1356
NlaIV GGNNCC 9 cut(s) 93, 604, 1043, 1084, 1288, 1421, 1576, 2796, 2885
NmeAIII GCCGAG 1 cut(s) 2682
NmuCI GTSAC 4 cut(s) 259, 700, 2572, 2700
NsbI TGCGCA 1 cut(s) 1844
NsiI ATGCAT 3 cut(s) 271, 487, 1356
NspI RCATGY 3 cut(s) 1260, 2212, 2239
NspV TTCGAA 2 cut(s) 224, 2481
OliI CACNNNNGTG 1 cut(s) 2570
PaeR7I CTCGAG 1 cut(s) 2324
PaqCI CACCTGC 1 cut(s) 688
PciSI GCTCTTC 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 2705
PdmI GAANNNNTTC 2 cut(s) 220, 2317
PfeI GAWTC 5 cut(s) 70, 460, 1685, 1942, 2719
PflFI GACNNNGTC 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 2006
PinAI ACCGGT 2 cut(s) 719, 1607
PkrI GCNGC 8 cut(s) 144, 958, 1177, 1262, 1692, 2101, 2122, 2241
PleI GAGTC 5 cut(s) 202, 298, 1475, 1931, 1997
PluTI GGCGCC 1 cut(s) 95
PmaCI CACGTG 2 cut(s) 278, 1100
PmlI CACGTG 2 cut(s) 278, 1100
PpsI GAGTC 5 cut(s) 202, 298, 1475, 1931, 1997
Ppu21I YACGTR 2 cut(s) 278, 1100
PpuMI RGGWCCY 2 cut(s) 1287, 1409
Psp124BI GAGCTC 2 cut(s) 2024, 2156
Psp5II RGGWCCY 2 cut(s) 1287, 1409
Psp6I CCWGG 4 cut(s) 249, 255, 1130, 2006
PspCI CACGTG 2 cut(s) 278, 1100
PspEI GGTNACC 1 cut(s) 2456
PspFI CCCAGC 2 cut(s) 131, 599
PspGI CCWGG 4 cut(s) 249, 255, 1130, 2006
PspN4I GGNNCC 9 cut(s) 93, 604, 1043, 1084, 1288, 1421, 1576, 2796, 2885
PspOMI GGGCCC 1 cut(s) 602
PspPI GGNCC 8 cut(s) 179, 253, 602, 603, 821, 1287, 1409, 2216
PspPPI RGGWCCY 2 cut(s) 1287, 1409
PspXI VCTCGAGB 1 cut(s) 2324
PsrI GAACNNNNNNTAC 2 cut(s) 1438, 1470
PsuI RGATCY 3 cut(s) 1041, 1276, 2883
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 4 cut(s) 28, 785, 1456, 2474
RsaNI GTAC 4 cut(s) 27, 784, 1455, 2473
RseI CAYNNNNRTG 2 cut(s) 1146, 2570
SacI GAGCTC 2 cut(s) 2024, 2156
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 3 cut(s) 560, 734, 1364
SatI GCNGC 8 cut(s) 143, 957, 1176, 1261, 1691, 2100, 2121, 2240
Sau96I GGNCC 8 cut(s) 179, 253, 602, 603, 821, 1287, 1409, 2216
SchI GAGTC 5 cut(s) 203, 299, 1475, 1931, 1997
ScrFI CCNGG 6 cut(s) 251, 257, 1132, 1995, 2008, 2214
SduI GDGCHC 7 cut(s) 606, 1422, 1568, 1630, 2024, 2156, 2379
SexAI ACCWGGT 1 cut(s) 255
SfaNI GCATC 6 cut(s) 197, 256, 382, 472, 494, 2718
SfcI CTRYAG 3 cut(s) 642, 1032, 2888
SfoI GGCGCC 1 cut(s) 93
Sfr274I CTCGAG 1 cut(s) 2324
SfuI TTCGAA 2 cut(s) 224, 2481
SinI GGWCC 6 cut(s) 179, 253, 821, 1287, 1409, 2216
SlaI CTCGAG 1 cut(s) 2324
SmiMI CAYNNNNRTG 2 cut(s) 1146, 2570
SmlI CTYRAG 5 cut(s) 928, 1582, 1629, 2324, 2378
SmoI CTYRAG 5 cut(s) 928, 1582, 1629, 2324, 2378
SspDI GGCGCC 1 cut(s) 91
SstI GAGCTC 2 cut(s) 2024, 2156
StyD4I CCNGG 6 cut(s) 249, 255, 1130, 1993, 2006, 2212
StyI CCWWGG 2 cut(s) 182, 824
TaaI ACNGT 7 cut(s) 39, 757, 1067, 1381, 2117, 2842, 2852
TaiI ACGT 7 cut(s) 280, 1102, 1364, 2053, 2287, 2478, 2702
TatI WGTACW 2 cut(s) 26, 1454
TauI GCSGC 2 cut(s) 1693, 2102
TfiI GAWTC 5 cut(s) 70, 460, 1685, 1942, 2719
Tru1I TTAA 3 cut(s) 560, 734, 1364
Tru9I TTAA 3 cut(s) 560, 734, 1364
TscAI CASTG 8 cut(s) 1319, 1558, 2110, 2122, 2379, 2683, 2790, 2879
TseFI GTSAC 4 cut(s) 259, 700, 2572, 2700
TseI GCWGC 6 cut(s) 142, 956, 1175, 1260, 2120, 2239
Tsp45I GTSAC 4 cut(s) 259, 700, 2572, 2700
TspDTI ATGAA 7 cut(s) 99, 417, 851, 1803, 1830, 1909, 2732
TspGWI ACGGA 1 cut(s) 1311
TspRI CASTG 8 cut(s) 1319, 1558, 2110, 2122, 2379, 2683, 2790, 2879
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 6 cut(s) 179, 253, 821, 1287, 1409, 2216
XagI CCTNNNNNAGG 1 cut(s) 254
XapI RAATTY 7 cut(s) 842, 892, 944, 1635, 1790, 2391, 2856
XbaI TCTAGA 2 cut(s) 53, 286
XceI RCATGY 3 cut(s) 1260, 2212, 2239
XhoI CTCGAG 1 cut(s) 2324
XmiI GTMKAC 1 cut(s) 1729
XmnI GAANNNNTTC 2 cut(s) 220, 2317
Zsp2I ATGCAT 3 cut(s) 271, 487, 1356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.