FvH4_5g10710

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001398
Physical Location & Seq
Forward (+)
6080524 .. 6083108
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10710.t1

Sequence Viewer

Length: 1467 bp
ATGGGAAAGAAAAGTGGTAGTACTTCATGGTTAACTATTGTCAAAAGGGCCTTCAGGTCTCCGTCTAAGGATCATGACCAAAAGAGCAGCAGAAGAAGAGAAGACCATGAACCAGAAGAGGAAGAGAAGAAAAGAGAGAAGAGAAGATGGCTATTTCGAAAGGCTACTTCACATATTCAACAATGTGAAGCAACAAAGACAGAAACAACCATATCTGTGACTCCTGTTTTGGCTGCTGACCAAAAACATGCATTTGCAGTGGCCGCAGCCACTGCAGCTGCGGCCGAAGCAGCTGTTGCTACTGCCCAAGCGGCAGTAGAAATTGTTCGGTTCACTAGGCCGAACAATTTTGCCAAAGAGCACTATGCGGCTACACTCATTCAAACAGCTTTCAGAGGTTACTTAGCGAGGAGAGCTCTGCGTGCGCTTAAGGGTCTCGTAAAGCTTCAGGCTTTAGTGAGAGGTCACAATGTTAGGAAGCAAGCAAAGCTGACACTTAAATGCATGCAAGCTCTGGTTCGAGTTCAAGATCAGGTTCGAAACGAAAGAGCCAGGCTTTCGCATGAAGGAGGCAGAAACTCCATGCTTTCCGAAACAGAAATGAGCTTATGGGAGTCCAGATACCTTCAAGACATTCGCGACAGAAAGTCGATATCCAGAGAAAAAGTGAGTGAAGGAGTTGATCAATTCTTGTTACAAAGTCGAAAGGAAGCTTCTTTAAAACGTGAAAAAGCCCTTGCTTATGCCTTTTCTCACCAGATATGGAGGCCTAGAAGACACCCCTCTATAGGGGAAGAAGCAGAGCTAGAAGAGAGAACAAAATGGCTTGATCGGTGGATGGCCACAAAGAACTGGGAAAATTACAGAGCTTCAACAGACAAAAGAGACTCCATAAAAACAGTGGAAATGGACACTTCTAGGCCTTACATTTCATCCCAACACCAAAAACAGCCAAATCCAAATGCTGCAATGGCTTCACCTTTGCACAAGTCACATTACAACAACATGAACATCTCTCTCGGCCACTCTCCTGCAACACCCTCTCCTTCAAAACCCAGAGCGCTAAAAGTGCGTTCCGCAAGTCCTAGATGCTCTTCAGCAGCACATACCCCCAATTTAGGCTCTGCTTACTGTTTCAGGGGTGGTATGTGTAGATATGGTGTCGGCTCAAATAATGTGATTGGTTCAGTGCCTAATTACATGGCTGCAACCGAGTCTGCAAAGGCTAAGGCGCGGTCTCTAAGTGCACCACGGTCAAGGCCAGCTACACCGGATAGAGACCGGGGTGGTTCGGCCAAGAAACGGCTGTCGTACCCGGCCCCTGAGTCACATAGCTTGGAAAGTCCTAGCTTCAAGAGTGTGCAAAATGGGTACTATGGAAGGGAGAACTTGTCTACTTACACTGATAGCCTAGGGGGAGAAATTTCTCCATGTTCAACCACTGACTTAAGGTGGTTACAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

489

Amino Acids

54.85

Weight (kDa)

10.12

Isoelectric Point (pI)

63.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 364 - 447 3.8e-20 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1394
AccII CGCG 2 cut(s) 639, 1234
AciI CCGC 6 cut(s) 264, 281, 311, 368, 1077, 1234
AclWI GGATC 1 cut(s) 78
AcoI YGGCCR 5 cut(s) 261, 282, 840, 1021, 1293
AcsI RAATTY 1 cut(s) 1422
AcuI CTGAAG 3 cut(s) 37, 431, 1080
AfaI GTAC 3 cut(s) 22, 1313, 1373
AfeI AGCGCT 1 cut(s) 1062
AfiI CCNNNNNNNGG 4 cut(s) 788, 789, 1118, 1302
AflII CTTAAG 2 cut(s) 428, 1447
AgsI TTSAA 8 cut(s) 179, 383, 527, 629, 873, 1050, 1354, 1437
AhdI GACNNNNNGTC 1 cut(s) 646
AjnI CCWGG 1 cut(s) 551
Alw21I GWGCWC 3 cut(s) 363, 418, 1249
Alw26I GTCTC 5 cut(s) 63, 440, 879, 1242, 1272
Alw44I GTGCAC 1 cut(s) 1245
AlwI GGATC 1 cut(s) 78
AlwNI CAGNNNCTG 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 1062
ApaLI GTGCAC 1 cut(s) 1245
ApeKI GCWGC 9 cut(s) 87, 233, 266, 275, 278, 290, 965, 1100, 1205
ApoI RAATTY 1 cut(s) 1422
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
Asp700I GAANNNNTTC 1 cut(s) 324
AspA2I CCTAGG 1 cut(s) 1411
AspLEI GCGC 3 cut(s) 427, 1063, 1234
AspS9I GGNCC 2 cut(s) 48, 1318
AsuC2I CCSGG 2 cut(s) 1283, 1316
AsuHPI GGTGA 2 cut(s) 746, 969
AsuII TTCGAA 2 cut(s) 157, 538
AvrII CCTAGG 1 cut(s) 1411
BaeGI GKGCMC 1 cut(s) 1249
BalI TGGCCA 1 cut(s) 842
BanII GRGCYC 1 cut(s) 418
BbsI GAAGAC 2 cut(s) 108, 781
Bbv12I GWGCWC 3 cut(s) 363, 418, 1249
BbvI GCAGC 9 cut(s) 99, 220, 265, 278, 287, 302, 952, 1112, 1192
BccI CCATC 2 cut(s) 141, 832
BceAI ACGGC 1 cut(s) 1319
BciT130I CCWGG 1 cut(s) 553
BclI TGATCA 1 cut(s) 682
BcnI CCSGG 2 cut(s) 1283, 1316
BcoDI GTCTC 5 cut(s) 63, 440, 879, 1242, 1272
BfaI CTAG 7 cut(s) 336, 771, 806, 918, 1086, 1347, 1412
BfmI CTRYAG 2 cut(s) 273, 786
BfoI RGCGCY 1 cut(s) 1064
BfrI CTTAAG 2 cut(s) 428, 1447
BglI GCCNNNNNGGC 1 cut(s) 311
BlnI CCTAGG 1 cut(s) 1411
BmcAI AGTACT 1 cut(s) 22
Bme1390I CCNGG 3 cut(s) 553, 1283, 1316
BmeRI GACNNNNNGTC 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 48, 1318
BmiI GGNNCC 1 cut(s) 1320
BmrFI CCNGG 3 cut(s) 553, 1283, 1316
BmrI ACTGGG 1 cut(s) 862
BmsI GCATC 1 cut(s) 1079
BmuI ACTGGG 1 cut(s) 862
BpiI GAAGAC 2 cut(s) 108, 781
BplI GAGNNNNNCTC 2 cut(s) 400, 432
Bpu10I CCTNAGC 1 cut(s) 1227
Bpu14I TTCGAA 2 cut(s) 157, 538
BpuMI CCSGG 2 cut(s) 1283, 1316
BsaI GGTCTC 4 cut(s) 63, 440, 1242, 1272
BsaJI CCNNGG 3 cut(s) 1250, 1282, 1411
BsaWI WCCGGW 1 cut(s) 1270
BsaXI ACNNNNNCTCC 4 cut(s) 1027, 1057, 1376, 1406
Bsc4I CCNNNNNNNGG 4 cut(s) 788, 789, 1118, 1302
Bse1I ACTGG 1 cut(s) 857
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 1 cut(s) 553
BseDI CCNNGG 3 cut(s) 1250, 1282, 1411
BseGI GGATG 2 cut(s) 843, 932
BseLI CCNNNNNNNGG 4 cut(s) 788, 789, 1118, 1302
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 1 cut(s) 1314
BseNI ACTGG 1 cut(s) 857
BseRI GAGGAG 1 cut(s) 424
BseSI GKGCMC 1 cut(s) 1249
BseX3I CGGCCG 1 cut(s) 282
BseXI GCAGC 9 cut(s) 99, 220, 265, 278, 287, 302, 952, 1112, 1192
Bsh1236I CGCG 2 cut(s) 639, 1234
Bsh1285I CGRYCG 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 285
BsiHKAI GWGCWC 3 cut(s) 363, 418, 1249
BsiSI CCGG 3 cut(s) 1271, 1282, 1316
BslI CCNNNNNNNGG 4 cut(s) 788, 789, 1118, 1302
BsmAI GTCTC 5 cut(s) 63, 440, 879, 1242, 1272
Bso31I GGTCTC 4 cut(s) 63, 440, 1242, 1272
Bsp119I TTCGAA 2 cut(s) 157, 538
Bsp1286I GDGCHC 3 cut(s) 363, 418, 1249
Bsp143I GATC 4 cut(s) 70, 529, 682, 829
Bsp68I TCGCGA 1 cut(s) 639
BspACI CCGC 6 cut(s) 264, 281, 311, 368, 1077, 1234
BspCNI CTCAG 1 cut(s) 1315
BspFNI CGCG 2 cut(s) 639, 1234
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 1320
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 1099
BspT104I TTCGAA 2 cut(s) 157, 538
BspTI CTTAAG 2 cut(s) 428, 1447
BspTNI GGTCTC 4 cut(s) 63, 440, 1242, 1272
BsrDI GCAATG 1 cut(s) 975
BsrI ACTGG 1 cut(s) 857
BssECI CCNNGG 3 cut(s) 1250, 1282, 1411
BssMI GATC 4 cut(s) 70, 529, 682, 829
BssT1I CCWWGG 1 cut(s) 1411
Bst2UI CCWGG 1 cut(s) 553
Bst4CI ACNGT 3 cut(s) 901, 1133, 1254
Bst6I CTCTTC 6 cut(s) 91, 111, 117, 134, 804, 1099
BstAFI CTTAAG 2 cut(s) 428, 1447
BstAPI GCANNNNNTGC 2 cut(s) 272, 296
BstBI TTCGAA 2 cut(s) 157, 538
BstC8I GCNNGC 5 cut(s) 423, 483, 506, 510, 1263
BstDEI CTNAG 5 cut(s) 66, 403, 1227, 1241, 1323
BstDSI CCRYGG 1 cut(s) 1250
BstF5I GGATG 2 cut(s) 843, 932
BstFNI CGCG 2 cut(s) 639, 1234
BstH2I RGCGCY 1 cut(s) 1064
BstHHI GCGC 3 cut(s) 427, 1063, 1234
BstKTI GATC 4 cut(s) 73, 532, 685, 832
BstMAI GTCTC 5 cut(s) 63, 440, 879, 1242, 1272
BstMBI GATC 4 cut(s) 70, 529, 682, 829
BstMCI CGRYCG 1 cut(s) 285
BstNI CCWGG 1 cut(s) 553
BstNSI RCATGY 2 cut(s) 251, 508
BstSCI CCNGG 3 cut(s) 551, 1281, 1314
BstSFI CTRYAG 2 cut(s) 273, 786
BstSLI GKGCMC 1 cut(s) 1249
BstUI CGCG 2 cut(s) 639, 1234
BstV1I GCAGC 9 cut(s) 99, 220, 265, 278, 287, 302, 952, 1112, 1192
BstV2I GAAGAC 2 cut(s) 108, 781
BstZI CGGCCG 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 1250
BtsCI GGATG 2 cut(s) 843, 932
BtsI GCAGTG 2 cut(s) 264, 270
BtsIMutI CAGTG 6 cut(s) 264, 270, 906, 1194, 1401, 1440
BtuMI TCGCGA 1 cut(s) 639
Cac8I GCNNGC 5 cut(s) 423, 483, 506, 510, 1263
CaiI CAGNNNCTG 1 cut(s) 272
CciI TCATGA 1 cut(s) 73
CfoI GCGC 3 cut(s) 427, 1063, 1234
Cfr13I GGNCC 2 cut(s) 48, 1318
Csp6I GTAC 3 cut(s) 21, 1312, 1372
CviQI GTAC 3 cut(s) 21, 1312, 1372
DdeI CTNAG 5 cut(s) 66, 403, 1227, 1241, 1323
DpnI GATC 4 cut(s) 72, 531, 684, 831
DpnII GATC 4 cut(s) 70, 529, 682, 829
DraI TTTAAA 1 cut(s) 720
DriI GACNNNNNGTC 1 cut(s) 646
EaeI YGGCCR 5 cut(s) 261, 282, 840, 1021, 1293
EagI CGGCCG 1 cut(s) 282
Eam1104I CTCTTC 6 cut(s) 91, 111, 117, 134, 804, 1099
Eam1105I GACNNNNNGTC 1 cut(s) 646
EarI CTCTTC 6 cut(s) 91, 111, 117, 134, 804, 1099
Ecl136II GAGCTC 1 cut(s) 416
EclXI CGGCCG 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 1411
Eco147I AGGCCT 2 cut(s) 769, 922
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 4 cut(s) 63, 440, 1242, 1272
Eco32I GATATC 1 cut(s) 654
Eco47III AGCGCT 1 cut(s) 1062
Eco52I CGGCCG 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 3 cut(s) 37, 431, 1080
EcoICRI GAGCTC 1 cut(s) 416
EcoO109I RGGNCCY 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 551
EcoRV GATATC 1 cut(s) 654
EcoT14I CCWWGG 1 cut(s) 1411
EcoT22I ATGCAT 2 cut(s) 253, 506
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 1411
FbaI TGATCA 1 cut(s) 682
FblI GTMKAC 1 cut(s) 1394
FokI GGATG 2 cut(s) 850, 919
FriOI GRGCYC 1 cut(s) 418
FspBI CTAG 7 cut(s) 336, 771, 806, 918, 1086, 1347, 1412
GlaI GCGC 3 cut(s) 426, 1062, 1233
HaeII RGCGCY 1 cut(s) 1064
HapII CCGG 3 cut(s) 1271, 1282, 1316
HhaI GCGC 3 cut(s) 427, 1063, 1234
Hin6I GCGC 3 cut(s) 425, 1061, 1232
HinP1I GCGC 3 cut(s) 425, 1061, 1232
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 2 cut(s) 443, 711
HinfI GANTC 5 cut(s) 220, 614, 887, 1214, 1325
HpaI GTTAAC 1 cut(s) 33
HpaII CCGG 3 cut(s) 1271, 1282, 1316
HphI GGTGA 2 cut(s) 746, 969
Hpy166II GTNNAC 4 cut(s) 33, 333, 1247, 1395
Hpy188I TCNGA 2 cut(s) 395, 592
Hpy188III TCNNGA 7 cut(s) 74, 527, 618, 629, 638, 657, 1354
Hpy8I GTNNAC 4 cut(s) 33, 333, 1247, 1395
HpyAV CCTTC 6 cut(s) 61, 560, 635, 668, 1056, 1374
HpyCH4III ACNGT 3 cut(s) 901, 1133, 1254
HpyCH4IV ACGT 1 cut(s) 724
HpyF3I CTNAG 5 cut(s) 66, 403, 1227, 1241, 1323
HpySE526I ACGT 1 cut(s) 724
HspAI GCGC 3 cut(s) 425, 1061, 1232
Ksp22I TGATCA 1 cut(s) 682
KspAI GTTAAC 1 cut(s) 33
Kzo9I GATC 4 cut(s) 70, 529, 682, 829
LguI GCTCTTC 1 cut(s) 1099
Lsp1109I GCAGC 9 cut(s) 99, 220, 265, 278, 287, 302, 952, 1112, 1192
LweI GCATC 1 cut(s) 1079
MaeI CTAG 7 cut(s) 336, 771, 806, 918, 1086, 1347, 1412
MaeII ACGT 1 cut(s) 724
MaeIII GTNAC 7 cut(s) 217, 398, 464, 693, 990, 1326, 1455
MalI GATC 4 cut(s) 72, 531, 684, 831
MboI GATC 4 cut(s) 70, 529, 682, 829
MhlI GDGCHC 3 cut(s) 363, 418, 1249
MlsI TGGCCA 1 cut(s) 842
MluCI AATT 7 cut(s) 321, 346, 686, 859, 1114, 1195, 1422
MluNI TGGCCA 1 cut(s) 842
MlyI GAGTC 5 cut(s) 214, 623, 881, 1223, 1334
MnlI CCTC 8 cut(s) 112, 389, 402, 455, 563, 759, 793, 1051
Mox20I TGGCCA 1 cut(s) 842
Mph1103I ATGCAT 2 cut(s) 253, 506
MroXI GAANNNNTTC 1 cut(s) 324
MscI TGGCCA 1 cut(s) 842
MseI TTAA 5 cut(s) 32, 429, 498, 719, 1448
MslI CAYNNNNRTG 2 cut(s) 215, 499
Msp20I TGGCCA 1 cut(s) 842
MspA1I CMGCKG 2 cut(s) 278, 293
MspCI CTTAAG 2 cut(s) 428, 1447
MspI CCGG 3 cut(s) 1271, 1282, 1316
MspR9I CCNGG 3 cut(s) 553, 1283, 1316
MvaI CCWGG 1 cut(s) 553
MvnI CGCG 2 cut(s) 639, 1234
NciI CCSGG 2 cut(s) 1283, 1316
NdeII GATC 4 cut(s) 70, 529, 682, 829
NlaIV GGNNCC 1 cut(s) 1320
NmeAIII GCCGAG 1 cut(s) 999
NmuCI GTSAC 4 cut(s) 217, 464, 990, 1326
NruI TCGCGA 1 cut(s) 639
NsiI ATGCAT 2 cut(s) 253, 506
NspI RCATGY 2 cut(s) 251, 508
NspV TTCGAA 2 cut(s) 157, 538
PaeI GCATGC 1 cut(s) 508
PagI TCATGA 1 cut(s) 73
PceI AGGCCT 2 cut(s) 769, 922
PciSI GCTCTTC 1 cut(s) 1099
PdmI GAANNNNTTC 1 cut(s) 324
PleI GAGTC 5 cut(s) 214, 622, 881, 1222, 1333
PpsI GAGTC 5 cut(s) 214, 622, 881, 1222, 1333
Psp124BI GAGCTC 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 551
PspGI CCWGG 1 cut(s) 551
PspN4I GGNNCC 1 cut(s) 1320
PspPI GGNCC 2 cut(s) 48, 1318
PsrI GAACNNNNNNTAC 2 cut(s) 309, 341
PstI CTGCAG 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 272
PvuII CAGCTG 2 cut(s) 278, 293
RruI TCGCGA 1 cut(s) 639
RsaI GTAC 3 cut(s) 22, 1313, 1373
RsaNI GTAC 3 cut(s) 21, 1312, 1372
RseI CAYNNNNRTG 2 cut(s) 215, 499
SacI GAGCTC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 1099
SaqAI TTAA 5 cut(s) 32, 429, 498, 719, 1448
Sau3AI GATC 4 cut(s) 70, 529, 682, 829
Sau96I GGNCC 2 cut(s) 48, 1318
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 5 cut(s) 214, 623, 881, 1223, 1334
ScrFI CCNGG 3 cut(s) 553, 1283, 1316
SduI GDGCHC 3 cut(s) 363, 418, 1249
SfaNI GCATC 1 cut(s) 1079
SfcI CTRYAG 2 cut(s) 273, 786
SfuI TTCGAA 2 cut(s) 157, 538
SmiMI CAYNNNNRTG 2 cut(s) 215, 499
SmlI CTYRAG 2 cut(s) 428, 1447
SmoI CTYRAG 2 cut(s) 428, 1447
SphI GCATGC 1 cut(s) 508
Sse9I AATT 7 cut(s) 321, 346, 686, 859, 1114, 1195, 1422
SseBI AGGCCT 2 cut(s) 769, 922
SsiI CCGC 6 cut(s) 264, 281, 311, 368, 1077, 1234
SspMI CTAG 7 cut(s) 336, 771, 806, 918, 1086, 1347, 1412
SstI GAGCTC 1 cut(s) 418
StuI AGGCCT 2 cut(s) 769, 922
StyD4I CCNGG 3 cut(s) 551, 1281, 1314
StyI CCWWGG 1 cut(s) 1411
TaaI ACNGT 3 cut(s) 901, 1133, 1254
TaiI ACGT 1 cut(s) 727
TaqI TCGA 5 cut(s) 157, 520, 538, 650, 703
TasI AATT 7 cut(s) 321, 346, 686, 859, 1114, 1195, 1422
TatI WGTACW 1 cut(s) 20
TauI GCSGC 4 cut(s) 266, 284, 314, 371
Tru1I TTAA 5 cut(s) 32, 429, 498, 719, 1448
Tru9I TTAA 5 cut(s) 32, 429, 498, 719, 1448
TscAI CASTG 6 cut(s) 264, 277, 906, 1194, 1408, 1447
TseFI GTSAC 4 cut(s) 217, 464, 990, 1326
TseI GCWGC 9 cut(s) 87, 233, 266, 275, 278, 290, 965, 1100, 1205
Tsp45I GTSAC 4 cut(s) 217, 464, 990, 1326
TspDTI ATGAA 5 cut(s) 15, 123, 579, 921, 1022
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 6 cut(s) 264, 277, 906, 1194, 1408, 1447
Vha464I CTTAAG 2 cut(s) 428, 1447
VneI GTGCAC 1 cut(s) 1245
XapI RAATTY 1 cut(s) 1422
XceI RCATGY 2 cut(s) 251, 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XmaJI CCTAGG 1 cut(s) 1411
XmiI GTMKAC 1 cut(s) 1394
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 7 cut(s) 336, 771, 806, 918, 1086, 1347, 1412
ZrmI AGTACT 1 cut(s) 22
Zsp2I ATGCAT 2 cut(s) 253, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.