pycom07g15460

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001398
Physical Location & Seq
Reverse (-)
17785743 .. 17787839
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g15460.2

Sequence Viewer

Length: 1401 bp
ATGCTTGCTGTGGCGGTGGCCACGGCGGAGGCGGCAAAGGCTACCGCCCACGCAGCCGCTGAGGTAGCTCGGCTGACTAGGCCTTCCAATCATGCCAGAGAACACTACTACGCCATTGTTATCCAGACAGCTTTCAGAGGATACTTGGCAAGGAGGGCGCTTCGTGCGCTTAAAGGGCTTGTGAAGTTGCAGGCCTTGGTGAGAGGCCACAATGTGAGGAAGCAAGCAAAGATGACGCTTAAGTGCATGCAGGCTCTGGTTCGGGTGCAGGCTCGGGTGCTCGATCAGCGCACCAGGCTTTCTCACGAAGGCAGCAGACAGTCTGCGTTTAGTGACACCACTAGTAGCGTTTGGGAATCGCGGTATCTTCAAGACATTTCGGATAGGAAATCCATGCAGTCTAGAGAGGGAAGTAGCATAGCAGATGATTGGGATGAACGTCCTCACACCATTGAGGAAGTAAAAGCCATGTTGCAGCAACGGAAAGAAGCTGCAATGAAGAGTGAGAAATCGTTGTCCCAAGCCTTTTCTCAACAGCTATGGAGGAACAGAAGGACCCCGTCTATGGGAAATGAGGATGAGCTTGAAGAGAGACCGAAATGGCTCGTGCCAAAGCCATGGGAGACCAGAGGAAGACCTTCAACTGACCGGAGAGACCCCATTAAAACAGTCGAAATCGACACCCCTCAGCCTTATGTTAATTTACCACCAAACTACAGAAGATCAAGTGCAAGCCAATACCACCAACACCAACACCAACAACAAAGACCCAGTTCACCAAAACAAAGACCTGGCTCACCAAAACAAAGACCCAGCTCACCACTCCATAGAGATCGAGCCTACCAAAACCAACCCTTTCATCATCACTCCCCTATCACACCCTCACCATCAAAAACCCAGCCTTTCCTAGTCCGGTCTGCAAGCCCTCGTTGCGTTAGAGAAGATAGAAGCTCCAATGCATCTCAAACTCCAAGCCTTAGGTCCAACTATTTCTACACTGGTGCTGTGCACCAACAACATAGCAGCAGGGCGGGTAGTACAAGTAATAGTAGCATTGCCAATTCTGGTGCTGCGTTGCCTAATTACATGGCTGCAACCGAGTCTGCCAAGGCTCGGATACGCTCTCAGAGCGCACCACGGCAAAGGCCATCTACACCAGACCGGGAACGAGCAGCTGGATCAGCAAAGAAAAGGCTCTCGTTTCCAGCTCCTGATCCTTACGGCCTGGCAATGGGGTGTGGAGGTTATGGATACAGCTTGAGGAGTCCAAGCTTTAAGAGTGTGAGTGCATTCCATTTCGGCGTGGAGCAACAGTCTAACTATTCTTCTTGTTGCACTGAGAGTCATGGTGGTGAGATTTCCCCTTCTTCAACCAGTGACCTTAGAAGGTGGTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

467

Amino Acids

52.32

Weight (kDa)

10.41

Isoelectric Point (pI)

72.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 361
AciI CCGC 7 cut(s) 14, 26, 32, 45, 57, 361, 1031
AclWI GGATC 2 cut(s) 1186, 1208
AcoI YGGCCR 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 1 cut(s) 1039
AfiI CCNNNNNNNGG 4 cut(s) 565, 566, 1113, 1231
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 4 cut(s) 371, 587, 642, 1371
AhlI ACTAGT 1 cut(s) 341
AjnI CCWGG 3 cut(s) 293, 790, 1224
Alw21I GWGCWC 2 cut(s) 282, 1011
Alw26I GTCTC 3 cut(s) 586, 617, 648
Alw44I GTGCAC 1 cut(s) 1007
AlwI GGATC 2 cut(s) 1186, 1208
AlwNI CAGNNNCTG 3 cut(s) 59, 256, 1211
Ama87I CYCGRG 1 cut(s) 273
AoxI GGCC 6 cut(s) 18, 80, 192, 205, 1145, 1222
ApaLI GTGCAC 1 cut(s) 1007
ApeKI GCWGC 8 cut(s) 53, 312, 475, 491, 1023, 1070, 1091, 1172
Asp700I GAANNNNTTC 1 cut(s) 637
AspLEI GCGC 4 cut(s) 160, 169, 291, 1133
AspS9I GGNCC 2 cut(s) 555, 981
AsuC2I CCSGG 1 cut(s) 1163
AsuHPI GGTGA 6 cut(s) 211, 768, 789, 810, 876, 1364
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 2 cut(s) 555, 981
AxyI CCTNAGG 1 cut(s) 977
BaeGI GKGCMC 1 cut(s) 1011
BalI TGGCCA 1 cut(s) 20
BauI CACGAG 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 640
Bbv12I GWGCWC 2 cut(s) 282, 1011
BbvCI CCTCAGC 2 cut(s) 60, 687
BbvI GCAGC 8 cut(s) 65, 324, 478, 487, 1035, 1057, 1078, 1184
BccI CCATC 2 cut(s) 895, 1156
BceAI ACGGC 3 cut(s) 39, 1154, 1237
BciT130I CCWGG 3 cut(s) 295, 792, 1226
BciVI GTATCC 3 cut(s) 134, 1110, 1244
BcnI CCSGG 1 cut(s) 1163
BcoDI GTCTC 3 cut(s) 586, 617, 648
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 4 cut(s) 78, 342, 402, 908
BfmI CTRYAG 1 cut(s) 715
BfoI RGCGCY 1 cut(s) 161
BfrI CTTAAG 1 cut(s) 239
BfuI GTATCC 3 cut(s) 134, 1110, 1244
Bme1390I CCNGG 4 cut(s) 295, 792, 1163, 1226
Bme18I GGWCC 2 cut(s) 555, 981
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 555, 981
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 4 cut(s) 295, 792, 1163, 1226
BmrI ACTGGG 1 cut(s) 765
BmsI GCATC 1 cut(s) 968
BmuI ACTGGG 1 cut(s) 765
BpiI GAAGAC 1 cut(s) 640
Bpu10I CCTNAGC 2 cut(s) 60, 687
BpuEI CTTGAG 1 cut(s) 1279
BpuMI CCSGG 1 cut(s) 1163
BsaI GGTCTC 3 cut(s) 586, 617, 648
BsaJI CCNNGG 5 cut(s) 21, 195, 617, 1107, 1136
BsaWI WCCGGW 2 cut(s) 648, 912
BsaXI ACNNNNNCTCC 2 cut(s) 1298, 1328
Bsc4I CCNNNNNNNGG 4 cut(s) 565, 566, 1113, 1231
Bse1I ACTGG 3 cut(s) 771, 1003, 1374
Bse21I CCTNAGG 1 cut(s) 977
Bse3DI GCAATG 3 cut(s) 501, 1053, 1236
BseBI CCWGG 3 cut(s) 295, 792, 1226
BseDI CCNNGG 5 cut(s) 21, 195, 617, 1107, 1136
BseGI GGATG 2 cut(s) 439, 583
BseLI CCNNNNNNNGG 4 cut(s) 565, 566, 1113, 1231
BseMI GCAATG 3 cut(s) 501, 1053, 1236
BseMII CTCAG 4 cut(s) 51, 701, 1139, 1329
BseNI ACTGG 3 cut(s) 771, 1003, 1374
BseRI GAGGAG 1 cut(s) 1276
BseSI GKGCMC 1 cut(s) 1011
BseXI GCAGC 8 cut(s) 65, 324, 478, 487, 1035, 1057, 1078, 1184
BseYI CCCAGC 2 cut(s) 812, 897
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 361
BshFI GGCC 6 cut(s) 20, 82, 194, 207, 1147, 1224
BsiHKAI GWGCWC 2 cut(s) 282, 1011
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 3 cut(s) 649, 913, 1162
BslFI GGGAC 1 cut(s) 502
BslI CCNNNNNNNGG 4 cut(s) 565, 566, 1113, 1231
BsmAI GTCTC 3 cut(s) 586, 617, 648
BsmFI GGGAC 1 cut(s) 502
BsmI GAATGC 1 cut(s) 1289
BsnI GGCC 6 cut(s) 20, 82, 194, 207, 1147, 1224
Bso31I GGTCTC 3 cut(s) 586, 617, 648
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 2 cut(s) 282, 1011
Bsp143I GATC 5 cut(s) 283, 722, 832, 1178, 1213
Bsp19I CCATGG 1 cut(s) 617
BspACI CCGC 7 cut(s) 14, 26, 32, 45, 57, 361, 1031
BspANI GGCC 6 cut(s) 20, 82, 194, 207, 1147, 1224
BspCNI CTCAG 4 cut(s) 52, 700, 1138, 1330
BspFNI CGCG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 557
BspPI GGATC 2 cut(s) 1186, 1208
BspTI CTTAAG 1 cut(s) 239
BspTNI GGTCTC 3 cut(s) 586, 617, 648
BsrDI GCAATG 3 cut(s) 501, 1053, 1236
BsrI ACTGG 3 cut(s) 771, 1003, 1374
BssECI CCNNGG 5 cut(s) 21, 195, 617, 1107, 1136
BssMI GATC 5 cut(s) 283, 722, 832, 1178, 1213
BssSI CACGAG 1 cut(s) 605
BssT1I CCWWGG 3 cut(s) 195, 617, 1107
Bst2BI CACGAG 1 cut(s) 605
Bst2UI CCWGG 3 cut(s) 295, 792, 1226
Bst4CI ACNGT 3 cut(s) 321, 670, 1314
Bst6I CTCTTC 2 cut(s) 494, 582
BstAFI CTTAAG 1 cut(s) 239
BstC8I GCNNGC 8 cut(s) 6, 192, 225, 248, 252, 270, 733, 922
BstDEI CTNAG 6 cut(s) 60, 687, 977, 1125, 1338, 1382
BstDSI CCRYGG 3 cut(s) 21, 617, 1136
BstF5I GGATG 2 cut(s) 439, 583
BstFNI CGCG 1 cut(s) 361
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 4 cut(s) 160, 169, 291, 1133
BstKTI GATC 5 cut(s) 286, 725, 835, 1181, 1216
BstMAI GTCTC 3 cut(s) 586, 617, 648
BstMBI GATC 5 cut(s) 283, 722, 832, 1178, 1213
BstNI CCWGG 3 cut(s) 295, 792, 1226
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 4 cut(s) 293, 790, 1161, 1224
BstSFI CTRYAG 1 cut(s) 715
BstSLI GKGCMC 1 cut(s) 1011
BstUI CGCG 1 cut(s) 361
BstV1I GCAGC 8 cut(s) 65, 324, 478, 487, 1035, 1057, 1078, 1184
BstV2I GAAGAC 1 cut(s) 640
BstXI CCANNNNNNTGG 1 cut(s) 618
Bsu36I CCTNAGG 1 cut(s) 977
BsuI GTATCC 3 cut(s) 134, 1110, 1244
BsuRI GGCC 6 cut(s) 20, 82, 194, 207, 1147, 1224
BtgI CCRYGG 3 cut(s) 21, 617, 1136
BtsCI GGATG 2 cut(s) 439, 583
BtsIMutI CAGTG 3 cut(s) 996, 1335, 1381
Cac8I GCNNGC 8 cut(s) 6, 192, 225, 248, 252, 270, 733, 922
CaiI CAGNNNCTG 3 cut(s) 59, 256, 1211
CfoI GCGC 4 cut(s) 160, 169, 291, 1133
Cfr13I GGNCC 2 cut(s) 555, 981
CseI GACGC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 1038
CviAII CATG 7 cut(s) 92, 247, 394, 469, 618, 1087, 1346
CviQI GTAC 1 cut(s) 1038
DdeI CTNAG 6 cut(s) 60, 687, 977, 1125, 1338, 1382
DpnI GATC 5 cut(s) 285, 724, 834, 1180, 1215
DpnII GATC 5 cut(s) 283, 722, 832, 1178, 1213
DraIII CACNNNGTG 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 2 cut(s) 494, 582
EarI CTCTTC 2 cut(s) 494, 582
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 3 cut(s) 195, 617, 1107
Eco147I AGGCCT 2 cut(s) 82, 194
Eco31I GGTCTC 3 cut(s) 586, 617, 648
Eco47I GGWCC 2 cut(s) 555, 981
Eco81I CCTNAGG 1 cut(s) 977
Eco88I CYCGRG 1 cut(s) 273
EcoO109I RGGNCCY 1 cut(s) 555
EcoRII CCWGG 3 cut(s) 293, 790, 1224
EcoT14I CCWWGG 3 cut(s) 195, 617, 1107
EcoT22I ATGCAT 1 cut(s) 961
ErhI CCWWGG 3 cut(s) 195, 617, 1107
FaeI CATG 7 cut(s) 95, 250, 397, 472, 621, 1090, 1349
FaqI GGGAC 1 cut(s) 502
FatI CATG 7 cut(s) 91, 246, 393, 468, 617, 1086, 1345
FauI CCCGC 1 cut(s) 1024
FokI GGATG 2 cut(s) 446, 590
FspBI CTAG 4 cut(s) 78, 342, 402, 908
GlaI GCGC 4 cut(s) 159, 168, 290, 1132
GsaI CCCAGC 2 cut(s) 816, 901
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 6 cut(s) 20, 82, 194, 207, 1147, 1224
HapII CCGG 3 cut(s) 649, 913, 1162
HgaI GACGC 1 cut(s) 244
HhaI GCGC 4 cut(s) 160, 169, 291, 1133
Hin1II CATG 7 cut(s) 95, 250, 397, 472, 621, 1090, 1349
Hin6I GCGC 4 cut(s) 158, 167, 289, 1131
HinP1I GCGC 4 cut(s) 158, 167, 289, 1131
HindIII AAGCTT 1 cut(s) 1270
HinfI GANTC 4 cut(s) 356, 1100, 1264, 1342
HpaII CCGG 3 cut(s) 649, 913, 1162
HphI GGTGA 6 cut(s) 211, 768, 789, 810, 876, 1364
Hpy166II GTNNAC 2 cut(s) 776, 1009
Hpy188I TCNGA 4 cut(s) 137, 382, 1116, 1128
Hpy188III TCNNGA 5 cut(s) 124, 305, 371, 402, 1211
Hpy8I GTNNAC 2 cut(s) 776, 1009
HpyAV CCTTC 6 cut(s) 93, 302, 546, 648, 1374, 1380
HpyCH4III ACNGT 3 cut(s) 321, 670, 1314
HpyCH4IV ACGT 1 cut(s) 439
HpyF3I CTNAG 6 cut(s) 60, 687, 977, 1125, 1338, 1382
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 7 cut(s) 95, 250, 397, 472, 621, 1090, 1349
HspAI GCGC 4 cut(s) 158, 167, 289, 1131
Kzo9I GATC 5 cut(s) 283, 722, 832, 1178, 1213
LmnI GCTCC 3 cut(s) 956, 1213, 1306
Lsp1109I GCAGC 8 cut(s) 65, 324, 478, 487, 1035, 1057, 1078, 1184
LweI GCATC 1 cut(s) 968
MaeI CTAG 4 cut(s) 78, 342, 402, 908
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 2 cut(s) 332, 1376
MalI GATC 5 cut(s) 285, 724, 834, 1180, 1215
MboI GATC 5 cut(s) 283, 722, 832, 1178, 1213
MboII GAAGA 8 cut(s) 359, 511, 599, 645, 732, 953, 1317, 1359
MhlI GDGCHC 2 cut(s) 282, 1011
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 3 cut(s) 700, 1060, 1081
MluNI TGGCCA 1 cut(s) 20
MlyI GAGTC 3 cut(s) 1109, 1273, 1351
MmeI TCCRAC 1 cut(s) 1008
Mox20I TGGCCA 1 cut(s) 20
Mph1103I ATGCAT 1 cut(s) 961
MroXI GAANNNNTTC 1 cut(s) 637
MscI TGGCCA 1 cut(s) 20
MseI TTAA 5 cut(s) 171, 240, 663, 699, 1275
MslI CAYNNNNRTG 1 cut(s) 1350
Msp20I TGGCCA 1 cut(s) 20
MspA1I CMGCKG 2 cut(s) 59, 1175
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 3 cut(s) 649, 913, 1162
MspR9I CCNGG 4 cut(s) 295, 792, 1163, 1226
Mva1269I GAATGC 1 cut(s) 1289
MvaI CCWGG 3 cut(s) 295, 792, 1226
MvnI CGCG 1 cut(s) 361
NciI CCSGG 1 cut(s) 1163
NcoI CCATGG 1 cut(s) 617
NdeII GATC 5 cut(s) 283, 722, 832, 1178, 1213
NlaIII CATG 7 cut(s) 95, 250, 397, 472, 621, 1090, 1349
NlaIV GGNNCC 1 cut(s) 557
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 2 cut(s) 332, 1376
NsiI ATGCAT 1 cut(s) 961
NspI RCATGY 1 cut(s) 250
PaeI GCATGC 1 cut(s) 250
PceI AGGCCT 2 cut(s) 82, 194
PctI GAATGC 1 cut(s) 1289
PdmI GAANNNNTTC 1 cut(s) 637
PfeI GAWTC 1 cut(s) 356
PflFI GACNNNGTC 1 cut(s) 559
PleI GAGTC 3 cut(s) 1108, 1272, 1350
PpsI GAGTC 3 cut(s) 1108, 1272, 1350
PpuMI RGGWCCY 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 3 cut(s) 293, 790, 1224
PspFI CCCAGC 2 cut(s) 812, 897
PspGI CCWGG 3 cut(s) 293, 790, 1224
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 2 cut(s) 555, 981
PspPPI RGGWCCY 1 cut(s) 555
PstNI CAGNNNCTG 3 cut(s) 59, 256, 1211
PsyI GACNNNGTC 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 1175
RsaI GTAC 1 cut(s) 1039
RsaNI GTAC 1 cut(s) 1038
RseI CAYNNNNRTG 1 cut(s) 1350
SaqAI TTAA 5 cut(s) 171, 240, 663, 699, 1275
Sau3AI GATC 5 cut(s) 283, 722, 832, 1178, 1213
Sau96I GGNCC 2 cut(s) 555, 981
SchI GAGTC 3 cut(s) 1109, 1273, 1351
ScrFI CCNGG 4 cut(s) 295, 792, 1163, 1226
SduI GDGCHC 2 cut(s) 282, 1011
SfaNI GCATC 1 cut(s) 968
SfcI CTRYAG 1 cut(s) 715
SinI GGWCC 2 cut(s) 555, 981
SmiMI CAYNNNNRTG 1 cut(s) 1350
SmlI CTYRAG 2 cut(s) 239, 1258
SmoI CTYRAG 2 cut(s) 239, 1258
SpeI ACTAGT 1 cut(s) 341
SphI GCATGC 1 cut(s) 250
Sse9I AATT 3 cut(s) 700, 1060, 1081
SseBI AGGCCT 2 cut(s) 82, 194
SsiI CCGC 7 cut(s) 14, 26, 32, 45, 57, 361, 1031
SspMI CTAG 4 cut(s) 78, 342, 402, 908
StuI AGGCCT 2 cut(s) 82, 194
StyD4I CCNGG 4 cut(s) 293, 790, 1161, 1224
StyI CCWWGG 3 cut(s) 195, 617, 1107
TaaI ACNGT 3 cut(s) 321, 670, 1314
TaiI ACGT 1 cut(s) 442
TaqI TCGA 4 cut(s) 282, 672, 678, 835
TaqII GACCGA 1 cut(s) 610
TasI AATT 3 cut(s) 700, 1060, 1081
TatI WGTACW 1 cut(s) 1037
TauI GCSGC 2 cut(s) 35, 59
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 5 cut(s) 171, 240, 663, 699, 1275
Tru9I TTAA 5 cut(s) 171, 240, 663, 699, 1275
TscAI CASTG 3 cut(s) 1003, 1342, 1381
TseFI GTSAC 2 cut(s) 332, 1376
TseI GCWGC 8 cut(s) 53, 312, 475, 491, 1023, 1070, 1091, 1172
Tsp45I GTSAC 2 cut(s) 332, 1376
TspDTI ATGAA 3 cut(s) 450, 512, 848
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 3 cut(s) 1003, 1342, 1381
Tth111I GACNNNGTC 1 cut(s) 559
Vha464I CTTAAG 1 cut(s) 239
VneI GTGCAC 1 cut(s) 1007
VpaK11BI GGWCC 2 cut(s) 555, 981
XbaI TCTAGA 1 cut(s) 401
XceI RCATGY 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 637
XspI CTAG 4 cut(s) 78, 342, 402, 908
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.