Rh1DG290000

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001398
Physical Location & Seq
Reverse (-)
51307600 .. 51310509
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG290000.1

Sequence Viewer

Length: 1359 bp
ATGGCGACTGCTCAAGCGGCCGCCGTCGAAGTGGCTCGGCTGACCAAGCCATCTAACCATGCCAGAGAGCAACTTCAGTTTGCAGCCATTGTCATTCAGACCGCTTTTAGAGGTTACTTGGCAAGGAGGGCGCTTCGTGCGCTTAAAGGGCTTGTGAAGCTGCAAGCTTTGGTGAGAGGACATAATGTGAGGAAGCAAGCGAAGATGACTCTCAGGTGTATGCAAGCTCTGGTTCGAGTTCAAGCTCGGGTTCTTGATCAGAGAACTAGGCTTTCCCATGAAGGTAGTAGAAAATCGGCCTTTAGCGACACTAATAGCAATAGCGCTTGGGAATCGCGGTATCTTCAAGACTTTTCAGACCGAAAATCCATTCAGTCTAGAGAGGGAAGTAGTATAGCAGATGATTGGGATGAAAGGCCTCACACTATTGAGGAAGTAAAAGCAATGTTGCAACATAGAAAAGAAGCTGCAATGAAGCGTGAGAAAGCTTTATCCCACGCCTTTTCTCAACAGATATGGAGAAATGGGAGGAGCCCATCAATGGGGAATGAGGAAGAGCTTGAAGAGAAACCCAAATGGTTAGACAGATGGATGGCCCCAAAGCCATGGGAGAGCAGCAGAGGAAGAGCTTCTACTGACCACAAAGACCCCATCAAAACAGTTGAAATCGACACCTCGCAGCCTTATTCAAGTTTATCATCCAATTTCCGAAGATCGAATGCAAGCCAATACCACCAACAAAACCTACAGCAAAGACCTAGTTCACCACTCCATAGAGATAGATCCCACCAAAACCAACACTTCCACCACTCCCCCATCACACCCTCGCCATCAAAGAGCCGCCCTCCTCTCCAAGTGCGGTCTGCGAGCCCTCGTTGCGTTAGAGAAGACCGAAGCTCCAATGCGTCTCAAACCCCTAGCCTGAGGTCTAATTATTACTACAATGGTGGTGTTGTGCACCAACATAGCAGGGGCAGCACTAGCACTAGTGGCGGCAATGTTGGTGCTACATTGCCTAATTACATGGCAGCAACTGAATCTGCCAAGGCTCGAATTCGCTCCCAAAGTGCACCGAGGCAAAGGCCTTCGACACCTGACAGGGAGCGAGGAGGAGGAGTTGGATCGGTGAAGAAAAGACTTTCGTTTCCAGCTCCCGATCCTTATGGGGTTGTTGGAATGGGGGTTTATGCAGGAGGAGGTTATGGGCCTAGCTTGAGGAGTCCGAGCTTTAAGAGTGTGGTGAGTGGATCACACTTTGGGTTGGAGCAACAATCTAACTATTCTTCATGTTGTACTGAGAGTCATGGTGGTGAGGCCTCTCCTTCTTCAACTAGTGACCTTAGAAGGTGGTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

452

Amino Acids

50.43

Weight (kDa)

10.42

Isoelectric Point (pI)

70.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 290 - 391 2.4e-30 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 337
AciI CCGC 7 cut(s) 17, 21, 102, 337, 841, 859, 993
AclWI GGATC 4 cut(s) 777, 1129, 1151, 1255
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 1 cut(s) 1053
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 1 cut(s) 1294
AfeI AGCGCT 1 cut(s) 325
AfiI CCNNNNNNNGG 2 cut(s) 541, 542
AgsI TTSAA 6 cut(s) 242, 347, 563, 665, 690, 1329
AhlI ACTAGT 2 cut(s) 986, 1331
Alw21I GWGCWC 2 cut(s) 960, 1072
Alw26I GTCTC 1 cut(s) 912
Alw44I GTGCAC 2 cut(s) 956, 1068
AlwI GGATC 4 cut(s) 777, 1129, 1151, 1255
AlwNI CAGNNNCTG 1 cut(s) 1034
Ama87I CYCGRG 1 cut(s) 246
Aor51HI AGCGCT 1 cut(s) 325
AoxI GGCC 7 cut(s) 18, 297, 416, 594, 1082, 1205, 1314
ApaLI GTGCAC 2 cut(s) 956, 1068
ApeKI GCWGC 7 cut(s) 83, 160, 467, 615, 679, 975, 1028
ApoI RAATTY 1 cut(s) 1053
Asp700I GAANNNNTTC 1 cut(s) 628
AspLEI GCGC 3 cut(s) 133, 142, 326
AspS9I GGNCC 2 cut(s) 595, 1205
AsuHPI GGTGA 5 cut(s) 184, 756, 1138, 1252, 1322
AvaI CYCGRG 1 cut(s) 246
AxyI CCTNAGG 1 cut(s) 923
BaeGI GKGCMC 2 cut(s) 960, 1072
BanII GRGCYC 2 cut(s) 536, 872
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
BbsI GAAGAC 1 cut(s) 894
Bbv12I GWGCWC 2 cut(s) 960, 1072
BbvI GCAGC 7 cut(s) 95, 147, 454, 627, 691, 987, 1040
BccI CCATC 7 cut(s) 58, 544, 582, 586, 659, 824, 838
BceAI ACGGC 1 cut(s) 8
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 912
BcuI ACTAGT 2 cut(s) 986, 1331
BfaI CTAG 8 cut(s) 267, 378, 759, 918, 981, 987, 1209, 1332
BfmI CTRYAG 1 cut(s) 746
BfoI RGCGCY 2 cut(s) 134, 327
BmeT110I CYCGRG 1 cut(s) 246
BmgT120I GGNCC 2 cut(s) 595, 1205
BmiI GGNNCC 2 cut(s) 533, 597
BpiI GAAGAC 1 cut(s) 894
BplI GAGNNNNNCTC 2 cut(s) 829, 861
BpuEI CTTGAG 1 cut(s) 1234
BsaJI CCNNGG 3 cut(s) 605, 1044, 1073
BsaXI ACNNNNNCTCC 4 cut(s) 881, 911, 1101, 1131
Bsc4I CCNNNNNNNGG 2 cut(s) 541, 542
Bse21I CCTNAGG 1 cut(s) 923
Bse3DI GCAATG 4 cut(s) 450, 477, 1003, 1010
BseDI CCNNGG 3 cut(s) 605, 1044, 1073
BseGI GGATG 3 cut(s) 415, 597, 698
BseLI CCNNNNNNNGG 2 cut(s) 541, 542
BseMI GCAATG 4 cut(s) 450, 477, 1003, 1010
BseMII CTCAG 3 cut(s) 226, 914, 1287
BseRI GAGGAG 7 cut(s) 544, 837, 1122, 1125, 1128, 1209, 1231
BseSI GKGCMC 2 cut(s) 960, 1072
BseX3I CGGCCG 1 cut(s) 18
BseXI GCAGC 7 cut(s) 95, 147, 454, 627, 691, 987, 1040
Bsh1236I CGCG 1 cut(s) 337
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 7 cut(s) 20, 299, 418, 596, 1084, 1207, 1316
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 2 cut(s) 960, 1072
BsiHKCI CYCGRG 1 cut(s) 246
BslI CCNNNNNNNGG 2 cut(s) 541, 542
BsmAI GTCTC 1 cut(s) 912
BsmBI CGTCTC 1 cut(s) 912
BsmI GAATGC 1 cut(s) 724
BsnI GGCC 7 cut(s) 20, 299, 418, 596, 1084, 1207, 1316
BsoBI CYCGRG 1 cut(s) 246
Bsp1286I GDGCHC 4 cut(s) 536, 872, 960, 1072
Bsp143I GATC 6 cut(s) 256, 713, 782, 1121, 1156, 1247
Bsp19I CCATGG 1 cut(s) 605
BspACI CCGC 7 cut(s) 17, 21, 102, 337, 841, 859, 993
BspANI GGCC 7 cut(s) 20, 299, 418, 596, 1084, 1207, 1316
BspCNI CTCAG 3 cut(s) 225, 915, 1288
BspFNI CGCG 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 533, 597
BspPI GGATC 4 cut(s) 777, 1129, 1151, 1255
BspQI GCTCTTC 2 cut(s) 549, 619
BsrDI GCAATG 4 cut(s) 450, 477, 1003, 1010
BssECI CCNNGG 3 cut(s) 605, 1044, 1073
BssMI GATC 6 cut(s) 256, 713, 782, 1121, 1156, 1247
BssT1I CCWWGG 2 cut(s) 605, 1044
Bst4CI ACNGT 1 cut(s) 661
Bst6I CTCTTC 3 cut(s) 549, 558, 619
BstC8I GCNNGC 5 cut(s) 165, 198, 225, 724, 868
BstDEI CTNAG 4 cut(s) 212, 923, 1296, 1340
BstDSI CCRYGG 1 cut(s) 605
BstF5I GGATG 3 cut(s) 415, 597, 698
BstFNI CGCG 1 cut(s) 337
BstH2I RGCGCY 2 cut(s) 134, 327
BstHHI GCGC 3 cut(s) 133, 142, 326
BstKTI GATC 6 cut(s) 259, 716, 785, 1124, 1159, 1250
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 6 cut(s) 256, 713, 782, 1121, 1156, 1247
BstMCI CGRYCG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 746
BstSLI GKGCMC 2 cut(s) 960, 1072
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 7 cut(s) 95, 147, 454, 627, 691, 987, 1040
BstV2I GAAGAC 1 cut(s) 894
BstX2I RGATCY 1 cut(s) 782
BstXI CCANNNNNNTGG 1 cut(s) 606
BstYI RGATCY 1 cut(s) 782
BstZI CGGCCG 1 cut(s) 18
Bsu36I CCTNAGG 1 cut(s) 923
BsuRI GGCC 7 cut(s) 20, 299, 418, 596, 1084, 1207, 1316
BtgI CCRYGG 1 cut(s) 605
BtsCI GGATG 3 cut(s) 415, 597, 698
Cac8I GCNNGC 5 cut(s) 165, 198, 225, 724, 868
CaiI CAGNNNCTG 1 cut(s) 1034
CciNI GCGGCCGC 1 cut(s) 18
CfoI GCGC 3 cut(s) 133, 142, 326
Cfr13I GGNCC 2 cut(s) 595, 1205
CseI GACGC 1 cut(s) 894
Csp6I GTAC 1 cut(s) 1293
CviAII CATG 6 cut(s) 59, 278, 606, 1024, 1287, 1304
CviQI GTAC 1 cut(s) 1293
DdeI CTNAG 4 cut(s) 212, 923, 1296, 1340
DpnI GATC 6 cut(s) 258, 715, 784, 1123, 1158, 1249
DpnII GATC 6 cut(s) 256, 713, 782, 1121, 1156, 1247
EaeI YGGCCR 1 cut(s) 18
EagI CGGCCG 1 cut(s) 18
Eam1104I CTCTTC 3 cut(s) 549, 558, 619
EarI CTCTTC 3 cut(s) 549, 558, 619
EclXI CGGCCG 1 cut(s) 18
Eco130I CCWWGG 2 cut(s) 605, 1044
Eco147I AGGCCT 3 cut(s) 418, 1084, 1316
Eco24I GRGCYC 2 cut(s) 536, 872
Eco47III AGCGCT 1 cut(s) 325
Eco52I CGGCCG 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 59
Eco81I CCTNAGG 1 cut(s) 923
Eco88I CYCGRG 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 1053
EcoT14I CCWWGG 2 cut(s) 605, 1044
EcoT38I GRGCYC 2 cut(s) 536, 872
ErhI CCWWGG 2 cut(s) 605, 1044
Esp3I CGTCTC 1 cut(s) 912
FaeI CATG 6 cut(s) 62, 281, 609, 1027, 1290, 1307
FatI CATG 6 cut(s) 58, 277, 605, 1023, 1286, 1303
FbaI TGATCA 1 cut(s) 256
FokI GGATG 3 cut(s) 422, 604, 685
FriOI GRGCYC 2 cut(s) 536, 872
FspBI CTAG 8 cut(s) 267, 378, 759, 918, 981, 987, 1209, 1332
GlaI GCGC 3 cut(s) 132, 141, 325
HaeII RGCGCY 2 cut(s) 134, 327
HaeIII GGCC 7 cut(s) 20, 299, 418, 596, 1084, 1207, 1316
HgaI GACGC 1 cut(s) 894
HhaI GCGC 3 cut(s) 133, 142, 326
Hin1II CATG 6 cut(s) 62, 281, 609, 1027, 1290, 1307
Hin6I GCGC 3 cut(s) 131, 140, 324
HinP1I GCGC 3 cut(s) 131, 140, 324
HindIII AAGCTT 2 cut(s) 165, 486
HinfI GANTC 5 cut(s) 208, 332, 1037, 1219, 1300
HphI GGTGA 5 cut(s) 184, 756, 1138, 1252, 1322
Hpy166II GTNNAC 3 cut(s) 764, 958, 1070
Hpy188I TCNGA 5 cut(s) 99, 261, 358, 710, 1224
Hpy188III TCNNGA 4 cut(s) 254, 347, 378, 1154
Hpy8I GTNNAC 3 cut(s) 764, 958, 1070
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 4 cut(s) 275, 1095, 1332, 1338
HpyCH4III ACNGT 1 cut(s) 661
HpyCH4V TGCA 9 cut(s) 83, 163, 223, 451, 470, 722, 958, 1070, 1190
HpyF3I CTNAG 4 cut(s) 212, 923, 1296, 1340
Hsp92II CATG 6 cut(s) 62, 281, 609, 1027, 1290, 1307
HspAI GCGC 3 cut(s) 131, 140, 324
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 6 cut(s) 256, 713, 782, 1121, 1156, 1247
LguI GCTCTTC 2 cut(s) 549, 619
LmnI GCTCC 6 cut(s) 531, 902, 1064, 1102, 1156, 1264
LpnPI CCDG 9 cut(s) 76, 199, 215, 935, 955, 1084, 1107, 1161, 1176
Lsp1109I GCAGC 7 cut(s) 95, 147, 454, 627, 691, 987, 1040
MaeI CTAG 8 cut(s) 267, 378, 759, 918, 981, 987, 1209, 1332
MaeIII GTNAC 2 cut(s) 113, 1334
MalI GATC 6 cut(s) 258, 715, 784, 1123, 1158, 1249
MboI GATC 6 cut(s) 256, 713, 782, 1121, 1156, 1247
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 4 cut(s) 536, 872, 960, 1072
MluCI AATT 4 cut(s) 703, 931, 1018, 1053
MlyI GAGTC 3 cut(s) 202, 1228, 1309
MmeI TCCRAC 3 cut(s) 1099, 1153, 1242
MroXI GAANNNNTTC 1 cut(s) 628
MseI TTAA 2 cut(s) 144, 1230
MslI CAYNNNNRTG 1 cut(s) 1308
Mva1269I GAATGC 1 cut(s) 724
MvnI CGCG 1 cut(s) 337
NcoI CCATGG 1 cut(s) 605
NdeII GATC 6 cut(s) 256, 713, 782, 1121, 1156, 1247
NlaIII CATG 6 cut(s) 62, 281, 609, 1027, 1290, 1307
NlaIV GGNNCC 2 cut(s) 533, 597
NmeAIII GCCGAG 1 cut(s) 16
NmuCI GTSAC 1 cut(s) 1334
NotI GCGGCCGC 1 cut(s) 18
PceI AGGCCT 3 cut(s) 418, 1084, 1316
PciSI GCTCTTC 2 cut(s) 549, 619
PctI GAATGC 1 cut(s) 724
PdmI GAANNNNTTC 1 cut(s) 628
PfeI GAWTC 2 cut(s) 332, 1037
PleI GAGTC 3 cut(s) 202, 1227, 1308
PpsI GAGTC 3 cut(s) 202, 1227, 1308
PspN4I GGNNCC 2 cut(s) 533, 597
PspPI GGNCC 2 cut(s) 595, 1205
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 1 cut(s) 782
RsaI GTAC 1 cut(s) 1294
RsaNI GTAC 1 cut(s) 1293
RseI CAYNNNNRTG 1 cut(s) 1308
SapI GCTCTTC 2 cut(s) 549, 619
SaqAI TTAA 2 cut(s) 144, 1230
Sau3AI GATC 6 cut(s) 256, 713, 782, 1121, 1156, 1247
Sau96I GGNCC 2 cut(s) 595, 1205
SchI GAGTC 3 cut(s) 202, 1228, 1309
SduI GDGCHC 4 cut(s) 536, 872, 960, 1072
SfcI CTRYAG 1 cut(s) 746
SmiMI CAYNNNNRTG 1 cut(s) 1308
SmlI CTYRAG 2 cut(s) 12, 1213
SmoI CTYRAG 2 cut(s) 12, 1213
SpeI ACTAGT 2 cut(s) 986, 1331
Sse9I AATT 4 cut(s) 703, 931, 1018, 1053
SseBI AGGCCT 3 cut(s) 418, 1084, 1316
SsiI CCGC 7 cut(s) 17, 21, 102, 337, 841, 859, 993
SspMI CTAG 8 cut(s) 267, 378, 759, 918, 981, 987, 1209, 1332
StuI AGGCCT 3 cut(s) 418, 1084, 1316
StyI CCWWGG 2 cut(s) 605, 1044
TaaI ACNGT 1 cut(s) 661
TaqI TCGA 6 cut(s) 27, 235, 669, 716, 1051, 1088
TaqII GACCGA 2 cut(s) 375, 906
TasI AATT 4 cut(s) 703, 931, 1018, 1053
TatI WGTACW 1 cut(s) 1292
TauI GCSGC 4 cut(s) 20, 23, 843, 996
TfiI GAWTC 2 cut(s) 332, 1037
Tru1I TTAA 2 cut(s) 144, 1230
Tru9I TTAA 2 cut(s) 144, 1230
TseFI GTSAC 1 cut(s) 1334
TseI GCWGC 7 cut(s) 83, 160, 467, 615, 679, 975, 1028
Tsp45I GTSAC 1 cut(s) 1334
TspDTI ATGAA 4 cut(s) 294, 426, 488, 1275
VneI GTGCAC 2 cut(s) 956, 1068
XapI RAATTY 1 cut(s) 1053
XbaI TCTAGA 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 628
XspI CTAG 8 cut(s) 267, 378, 759, 918, 981, 987, 1209, 1332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.