MD01G1087900.v1.1

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001398
Physical Location & Seq
Reverse (-)
19776073 .. 19779183
3111 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1087900.v1.1.491

Sequence Viewer

Length: 1608 bp
ATGGGGAGGAAGGGCGGCACTTCCTGGTTGACTGCTGTCAAAAGGGCTTTCAGATCTCCCACTAAAGACGACGAAGATCAAAAGAAAAGAGAGAAACGAAGGTGGATTTTCAGAAAACCCACAAGTTTAAACCAACAAGAAACAGTGACCCAAGAAGCAGCCGCGGAGCAAAAGCATGTTCCAGCAGCTGCAGATCAAAAGCACGTGCTTTCTGTGGCGGTGGCCACGGCGGAGGCGGCAATGGCCACCGCCCACGCAGCCGCTGAAGTGGTTCGGCTGACTAGGCCATCCAATCACGCCAGAGAAAATTACTACGCCATTGTTATCCAGACAGCTTTCAGAGGATACTTGGCAAGGAGGGCGCTTCGTGCGCTTAAAGGGCTTGTGAAGTTGCAGGCATTGGTGAGAGGCCATAATGTCAGGAAACAAGCAAAGATGACGCTCAAGTGCATGCAGGCTCTGGTTCGAGTACAGTCTCGGGTGCTCGATCAGCGAACCAGGCTTTCTCATGAAGGCAGCAGAAAGTCTGCGTTTAGTGACACCACCAGTAGGGTTTGGGAATCGCAGTATCTTCAAGACATTTCGGATAGGAAGTCGCTGTCTAGAGAGGGAAGTAGCATAGCAGATGATTGGGACGAACGTCCTCACACCATCGAGGAAGTAAAAGCCATGTTGCAGCACAGGAAAGAAGCAGCCATGAAGCGTGAGAAATCGTTGTCCCAAGCCTTTTCTCAACAGATATGGAGGAACGGCAAGACCCCATCTATGGGAAATGAGGATGAGCTTGAAGAGAGACCGAAATGGCTAGATAGATGGATGGCCCCAAAGCCATGGGAGAGCAGAGGAAGAGCTTCAACTGACCTGAGAGACCATGTTAAAACAGTCGAAATGGACACTTCTCAGCCTTATTCAAGTTTAGCACCAAACTTCAGAAGATCAAGCGCAAGCCAATACCACCAAAACCAACAACAAAGACCCAGTTCACCAAAGCAAAGACCCGGCTCACCTAAACAAAGACCCAGCTCGCCACTCCATAGAGATAGAGCCTACCAAAGCCAACCCTTTCATCATCACTCCCCTATCACACCCTCCCCAACAAAAACCCGGCCTGTCCAAGTCCGGTCTGCCAGCCCTCTTTGCATTAGAGAAGATAGAAGCTCCAATGCATCTCAAACTCCAAGCCTTAGGTCCAACTATTTCTACACTGGTGCTCTGCACCAACAACATAGCAGCAGGGCTGGTAGTACAAGCAATAGTAGCATTGCCAATTCTGGTGTTGCATTGCCTAATTACATGGCTGCAACTGAGTCTGCCAAGGCTCGGATACGCTCTCAGAGTGCACCCCGGCAAAGGCCATCTACACCAGACCGGGAACGAGCAGCCGGATCAGCAAAGAAAAGGCTCTCGTTTCCAGCTCCTGATCCTCATGGCTTGGCAATTGGATATGGAGGTTATGGACACAGCTTGAGGAGTCCAAGCTTTAAGAGTGTGAGTGCATCGCATTTCGGCGTGGAGCAACAGTCTAACTATTCTTCTTGTTGCACTGAGAGTCATGGTGGTGAGGTTTCCCCTTGTTCAACCAGTGACCTTAGGAGGTGGCTAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

536

Amino Acids

60.09

Weight (kDa)

10.41

Isoelectric Point (pI)

69.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 377 - 491 2.2e-28 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 164
AciI CCGC 8 cut(s) 15, 162, 164, 218, 230, 236, 249, 261
AclWI GGATC 2 cut(s) 1393, 1415
AcoI YGGCCR 2 cut(s) 222, 243
AcuI CTGAAG 2 cut(s) 285, 913
AcvI CACGTG 1 cut(s) 205
AfaI GTAC 2 cut(s) 471, 1246
AfiI CCNNNNNNNGG 5 cut(s) 549, 766, 767, 1320, 1350
AgsI TTSAA 5 cut(s) 575, 788, 855, 912, 1578
AjnI CCWGG 2 cut(s) 23, 497
AluBI AGCT 9 cut(s) 188, 335, 784, 851, 1023, 1158, 1415, 1464, 1479
AluI AGCT 9 cut(s) 188, 335, 784, 851, 1023, 1158, 1415, 1464, 1479
Alw21I GWGCWC 3 cut(s) 486, 1213, 1342
Alw26I GTCTC 3 cut(s) 480, 787, 861
Alw44I GTGCAC 1 cut(s) 1338
AlwI GGATC 2 cut(s) 1393, 1415
AlwNI CAGNNNCTG 4 cut(s) 188, 263, 460, 1418
Ama87I CYCGRG 1 cut(s) 477
AoxI GGCC 7 cut(s) 222, 243, 284, 409, 819, 1106, 1352
ApaLI GTGCAC 1 cut(s) 1338
Asp700I GAANNNNTTC 2 cut(s) 270, 850
AspLEI GCGC 3 cut(s) 364, 373, 944
AspS9I GGNCC 2 cut(s) 820, 1188
AsuC2I CCSGG 4 cut(s) 999, 1105, 1345, 1370
AsuHPI GGTGA 4 cut(s) 415, 975, 996, 1571
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 1 cut(s) 1188
AxyI CCTNAGG 2 cut(s) 1184, 1589
BaeGI GKGCMC 1 cut(s) 1342
BalI TGGCCA 2 cut(s) 224, 245
BbrPI CACGTG 1 cut(s) 205
Bbv12I GWGCWC 3 cut(s) 486, 1213, 1342
BccI CCATC 6 cut(s) 295, 659, 769, 807, 811, 1363
BceAI ACGGC 2 cut(s) 243, 766
BciT130I CCWGG 2 cut(s) 25, 499
BciVI GTATCC 2 cut(s) 338, 1317
BcnI CCSGG 4 cut(s) 999, 1105, 1345, 1370
BcoDI GTCTC 3 cut(s) 480, 787, 861
BfaI CTAG 3 cut(s) 282, 603, 806
BfmI CTRYAG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 365
BfuI GTATCC 2 cut(s) 338, 1317
BglII AGATCT 1 cut(s) 53
Bme1390I CCNGG 6 cut(s) 25, 499, 999, 1105, 1345, 1370
Bme18I GGWCC 1 cut(s) 1188
BmeT110I CYCGRG 1 cut(s) 477
BmgT120I GGNCC 2 cut(s) 820, 1188
BmiI GGNNCC 1 cut(s) 822
BmrFI CCNGG 6 cut(s) 25, 499, 999, 1105, 1345, 1370
BmrI ACTGGG 1 cut(s) 972
BmsI GCATC 2 cut(s) 1175, 1505
BmuI ACTGGG 1 cut(s) 972
BoxI GACNNNNGTC 2 cut(s) 35, 639
Bpu10I CCTNAGC 1 cut(s) 1601
BpuEI CTTGAG 2 cut(s) 428, 1486
BpuMI CCSGG 4 cut(s) 999, 1105, 1345, 1370
BsaAI YACGTR 1 cut(s) 205
BsaI GGTCTC 2 cut(s) 787, 861
BsaJI CCNNGG 5 cut(s) 162, 225, 830, 1314, 1343
BsaWI WCCGGW 1 cut(s) 1119
BsaXI ACNNNNNCTCC 2 cut(s) 1505, 1535
Bsc4I CCNNNNNNNGG 5 cut(s) 549, 766, 767, 1320, 1350
Bse1I ACTGG 4 cut(s) 546, 978, 1210, 1581
Bse21I CCTNAGG 2 cut(s) 1184, 1589
Bse3DI GCAATG 3 cut(s) 246, 1260, 1280
BseBI CCWGG 2 cut(s) 25, 499
BseDI CCNNGG 5 cut(s) 162, 225, 830, 1314, 1343
BseGI GGATG 3 cut(s) 287, 784, 822
BseLI CCNNNNNNNGG 5 cut(s) 549, 766, 767, 1320, 1350
BseMI GCAATG 3 cut(s) 246, 1260, 1280
BseMII CTCAG 5 cut(s) 854, 914, 1296, 1346, 1536
BseNI ACTGG 4 cut(s) 546, 978, 1210, 1581
BseRI GAGGAG 1 cut(s) 1483
BseSI GKGCMC 1 cut(s) 1342
BseYI CCCAGC 1 cut(s) 1019
BsgI GTGCAG 1 cut(s) 1199
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 7 cut(s) 224, 245, 286, 411, 821, 1108, 1354
BsiHKAI GWGCWC 3 cut(s) 486, 1213, 1342
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 6 cut(s) 999, 1105, 1120, 1345, 1369, 1383
BslFI GGGAC 2 cut(s) 647, 703
BslI CCNNNNNNNGG 5 cut(s) 549, 766, 767, 1320, 1350
BsmAI GTCTC 3 cut(s) 480, 787, 861
BsmFI GGGAC 2 cut(s) 647, 703
BsnI GGCC 7 cut(s) 224, 245, 286, 411, 821, 1108, 1354
Bso31I GGTCTC 2 cut(s) 787, 861
BsoBI CYCGRG 1 cut(s) 477
Bsp1286I GDGCHC 3 cut(s) 486, 1213, 1342
Bsp143I GATC 7 cut(s) 53, 76, 193, 487, 935, 1385, 1420
Bsp19I CCATGG 1 cut(s) 830
BspACI CCGC 8 cut(s) 15, 162, 164, 218, 230, 236, 249, 261
BspANI GGCC 7 cut(s) 224, 245, 286, 411, 821, 1108, 1354
BspCNI CTCAG 5 cut(s) 855, 913, 1297, 1345, 1537
BspFNI CGCG 1 cut(s) 164
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 1 cut(s) 822
BspMAI CTGCAG 1 cut(s) 193
BspPI GGATC 2 cut(s) 1393, 1415
BspQI GCTCTTC 1 cut(s) 841
BspTNI GGTCTC 2 cut(s) 787, 861
BsrDI GCAATG 3 cut(s) 246, 1260, 1280
BsrI ACTGG 4 cut(s) 546, 978, 1210, 1581
BssECI CCNNGG 5 cut(s) 162, 225, 830, 1314, 1343
BssMI GATC 7 cut(s) 53, 76, 193, 487, 935, 1385, 1420
BssT1I CCWWGG 2 cut(s) 830, 1314
Bst2UI CCWGG 2 cut(s) 25, 499
Bst4CI ACNGT 4 cut(s) 145, 474, 883, 1521
Bst6I CTCTTC 2 cut(s) 783, 841
BstBAI YACGTR 1 cut(s) 205
BstC8I GCNNGC 6 cut(s) 396, 452, 456, 946, 1025, 1129
BstDEI CTNAG 8 cut(s) 863, 900, 1184, 1305, 1332, 1545, 1589, 1601
BstDSI CCRYGG 3 cut(s) 162, 225, 830
BstF5I GGATG 3 cut(s) 287, 784, 822
BstFNI CGCG 1 cut(s) 164
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 3 cut(s) 364, 373, 944
BstKTI GATC 7 cut(s) 56, 79, 196, 490, 938, 1388, 1423
BstMAI GTCTC 3 cut(s) 480, 787, 861
BstMBI GATC 7 cut(s) 53, 76, 193, 487, 935, 1385, 1420
BstNI CCWGG 2 cut(s) 25, 499
BstNSI RCATGY 2 cut(s) 179, 454
BstPAI GACNNNNGTC 2 cut(s) 35, 639
BstSCI CCNGG 6 cut(s) 23, 497, 997, 1103, 1343, 1368
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 1342
BstUI CGCG 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 53
BstXI CCANNNNNNTGG 1 cut(s) 831
BstYI RGATCY 1 cut(s) 53
Bsu36I CCTNAGG 2 cut(s) 1184, 1589
BsuI GTATCC 2 cut(s) 338, 1317
BsuRI GGCC 7 cut(s) 224, 245, 286, 411, 821, 1108, 1354
BtgI CCRYGG 3 cut(s) 162, 225, 830
BtgZI GCGATG 1 cut(s) 1482
BtsCI GGATG 3 cut(s) 287, 784, 822
BtsIMutI CAGTG 4 cut(s) 150, 1203, 1542, 1588
Cac8I GCNNGC 6 cut(s) 396, 452, 456, 946, 1025, 1129
CaiI CAGNNNCTG 4 cut(s) 188, 263, 460, 1418
CciI TCATGA 1 cut(s) 508
CfoI GCGC 3 cut(s) 364, 373, 944
Cfr13I GGNCC 2 cut(s) 820, 1188
Cfr42I CCGCGG 1 cut(s) 165
CseI GACGC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 470, 1245
CviQI GTAC 2 cut(s) 470, 1245
DdeI CTNAG 8 cut(s) 863, 900, 1184, 1305, 1332, 1545, 1589, 1601
DpnI GATC 7 cut(s) 55, 78, 195, 489, 937, 1387, 1422
DpnII GATC 7 cut(s) 53, 76, 193, 487, 935, 1385, 1420
DraI TTTAAA 1 cut(s) 129
EaeI YGGCCR 2 cut(s) 222, 243
Eam1104I CTCTTC 2 cut(s) 783, 841
EarI CTCTTC 2 cut(s) 783, 841
EciI GGCGGA 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 830, 1314
Eco31I GGTCTC 2 cut(s) 787, 861
Eco47I GGWCC 1 cut(s) 1188
Eco57I CTGAAG 2 cut(s) 285, 913
Eco72I CACGTG 1 cut(s) 205
Eco81I CCTNAGG 2 cut(s) 1184, 1589
Eco88I CYCGRG 1 cut(s) 477
EcoRII CCWGG 2 cut(s) 23, 497
EcoT14I CCWWGG 2 cut(s) 830, 1314
EcoT22I ATGCAT 1 cut(s) 1168
ErhI CCWWGG 2 cut(s) 830, 1314
FaqI GGGAC 2 cut(s) 647, 703
FokI GGATG 3 cut(s) 274, 791, 829
FspBI CTAG 3 cut(s) 282, 603, 806
GlaI GCGC 3 cut(s) 363, 372, 943
GsaI CCCAGC 1 cut(s) 1023
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 7 cut(s) 224, 245, 286, 411, 821, 1108, 1354
HapII CCGG 6 cut(s) 999, 1105, 1120, 1345, 1369, 1383
HgaI GACGC 1 cut(s) 448
HhaI GCGC 3 cut(s) 364, 373, 944
Hin6I GCGC 3 cut(s) 362, 371, 942
HinP1I GCGC 3 cut(s) 362, 371, 942
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HindIII AAGCTT 1 cut(s) 1477
HinfI GANTC 4 cut(s) 560, 1307, 1471, 1549
HpaII CCGG 6 cut(s) 999, 1105, 1120, 1345, 1369, 1383
HphI GGTGA 4 cut(s) 415, 975, 996, 1571
Hpy166II GTNNAC 3 cut(s) 30, 983, 1340
Hpy188I TCNGA 7 cut(s) 53, 113, 341, 586, 932, 1323, 1335
Hpy188III TCNNGA 6 cut(s) 328, 421, 509, 575, 603, 1418
Hpy8I GTNNAC 3 cut(s) 30, 983, 1340
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 3 cut(s) 4, 93, 506
HpyCH4III ACNGT 4 cut(s) 145, 474, 883, 1521
HpyCH4IV ACGT 2 cut(s) 204, 640
HpyF3I CTNAG 8 cut(s) 863, 900, 1184, 1305, 1332, 1545, 1589, 1601
HpySE526I ACGT 2 cut(s) 204, 640
HspAI GCGC 3 cut(s) 362, 371, 942
KspI CCGCGG 1 cut(s) 165
Kzo9I GATC 7 cut(s) 53, 76, 193, 487, 935, 1385, 1420
LguI GCTCTTC 1 cut(s) 841
LmnI GCTCC 4 cut(s) 166, 1163, 1420, 1513
LweI GCATC 2 cut(s) 1175, 1505
MaeI CTAG 3 cut(s) 282, 603, 806
MaeII ACGT 2 cut(s) 204, 640
MaeIII GTNAC 3 cut(s) 145, 536, 1583
MalI GATC 7 cut(s) 55, 78, 195, 489, 937, 1387, 1422
MboI GATC 7 cut(s) 53, 76, 193, 487, 935, 1385, 1420
MboII GAAGA 7 cut(s) 86, 563, 800, 858, 945, 1160, 1524
MfeI CAATTG 1 cut(s) 1437
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 3 cut(s) 486, 1213, 1342
MlsI TGGCCA 2 cut(s) 224, 245
MluCI AATT 4 cut(s) 307, 1267, 1288, 1437
MluNI TGGCCA 2 cut(s) 224, 245
MlyI GAGTC 3 cut(s) 1316, 1480, 1558
MmeI TCCRAC 1 cut(s) 1215
Mox20I TGGCCA 2 cut(s) 224, 245
Mph1103I ATGCAT 1 cut(s) 1168
MroXI GAANNNNTTC 2 cut(s) 270, 850
MscI TGGCCA 2 cut(s) 224, 245
MseI TTAA 4 cut(s) 128, 375, 876, 1482
MslI CAYNNNNRTG 1 cut(s) 1557
Msp20I TGGCCA 2 cut(s) 224, 245
MspA1I CMGCKG 3 cut(s) 164, 188, 263
MspI CCGG 6 cut(s) 999, 1105, 1120, 1345, 1369, 1383
MspR9I CCNGG 6 cut(s) 25, 499, 999, 1105, 1345, 1370
MssI GTTTAAAC 1 cut(s) 129
MunI CAATTG 1 cut(s) 1437
MvaI CCWGG 2 cut(s) 25, 499
MvnI CGCG 1 cut(s) 164
NciI CCSGG 4 cut(s) 999, 1105, 1345, 1370
NcoI CCATGG 1 cut(s) 830
NdeII GATC 7 cut(s) 53, 76, 193, 487, 935, 1385, 1420
NlaIV GGNNCC 1 cut(s) 822
NmuCI GTSAC 3 cut(s) 145, 536, 1583
NsiI ATGCAT 1 cut(s) 1168
NspI RCATGY 2 cut(s) 179, 454
PaeI GCATGC 1 cut(s) 454
PagI TCATGA 1 cut(s) 508
PciSI GCTCTTC 1 cut(s) 841
PdmI GAANNNNTTC 2 cut(s) 270, 850
PfeI GAWTC 1 cut(s) 560
PleI GAGTC 3 cut(s) 1315, 1479, 1557
PmaCI CACGTG 1 cut(s) 205
PmeI GTTTAAAC 1 cut(s) 129
PmlI CACGTG 1 cut(s) 205
PpsI GAGTC 3 cut(s) 1315, 1479, 1557
Ppu21I YACGTR 1 cut(s) 205
PshAI GACNNNNGTC 2 cut(s) 35, 639
Psp6I CCWGG 2 cut(s) 23, 497
PspCI CACGTG 1 cut(s) 205
PspFI CCCAGC 1 cut(s) 1019
PspGI CCWGG 2 cut(s) 23, 497
PspN4I GGNNCC 1 cut(s) 822
PspPI GGNCC 2 cut(s) 820, 1188
PstI CTGCAG 1 cut(s) 193
PstNI CAGNNNCTG 4 cut(s) 188, 263, 460, 1418
PsuI RGATCY 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 471, 1246
RsaNI GTAC 2 cut(s) 470, 1245
RseI CAYNNNNRTG 1 cut(s) 1557
SacII CCGCGG 1 cut(s) 165
SapI GCTCTTC 1 cut(s) 841
SaqAI TTAA 4 cut(s) 128, 375, 876, 1482
Sau3AI GATC 7 cut(s) 53, 76, 193, 487, 935, 1385, 1420
Sau96I GGNCC 2 cut(s) 820, 1188
SchI GAGTC 3 cut(s) 1316, 1480, 1558
ScrFI CCNGG 6 cut(s) 25, 499, 999, 1105, 1345, 1370
SduI GDGCHC 3 cut(s) 486, 1213, 1342
SfaNI GCATC 2 cut(s) 1175, 1505
SfcI CTRYAG 1 cut(s) 189
Sfr303I CCGCGG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 165
SinI GGWCC 1 cut(s) 1188
SmiMI CAYNNNNRTG 1 cut(s) 1557
SmlI CTYRAG 2 cut(s) 443, 1465
SmoI CTYRAG 2 cut(s) 443, 1465
SphI GCATGC 1 cut(s) 454
Sse9I AATT 4 cut(s) 307, 1267, 1288, 1437
SsiI CCGC 8 cut(s) 15, 162, 164, 218, 230, 236, 249, 261
SspMI CTAG 3 cut(s) 282, 603, 806
StyD4I CCNGG 6 cut(s) 23, 497, 997, 1103, 1343, 1368
StyI CCWWGG 2 cut(s) 830, 1314
TaaI ACNGT 4 cut(s) 145, 474, 883, 1521
TaiI ACGT 2 cut(s) 207, 643
TaqI TCGA 4 cut(s) 466, 486, 654, 885
TaqII GACCGA 1 cut(s) 811
TasI AATT 4 cut(s) 307, 1267, 1288, 1437
TatI WGTACW 2 cut(s) 469, 1244
TauI GCSGC 4 cut(s) 18, 164, 239, 263
TfiI GAWTC 1 cut(s) 560
Tru1I TTAA 4 cut(s) 128, 375, 876, 1482
Tru9I TTAA 4 cut(s) 128, 375, 876, 1482
TscAI CASTG 4 cut(s) 150, 1210, 1549, 1588
TseFI GTSAC 3 cut(s) 145, 536, 1583
Tsp45I GTSAC 3 cut(s) 145, 536, 1583
TspDTI ATGAA 3 cut(s) 525, 713, 1055
TspRI CASTG 4 cut(s) 150, 1210, 1549, 1588
VneI GTGCAC 1 cut(s) 1338
VpaK11BI GGWCC 1 cut(s) 1188
XbaI TCTAGA 1 cut(s) 602
XceI RCATGY 2 cut(s) 179, 454
XmnI GAANNNNTTC 2 cut(s) 270, 850
XspI CTAG 3 cut(s) 282, 603, 806
Zsp2I ATGCAT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.